Rh7DG488400

Beta-hexosaminidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009312
Physical Location & Seq
Forward (+)
69314220 .. 69337836
23617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG488400.1

Sequence Viewer

Length: 327 bp
ATGTTCTGGTGGCCAGCAGGAGTTGATTGGGGTGACCGACTCGCGTCCGAACCAGGAACAGGTCATCTAAATCCTTTGAACCCCAAGACCTATCAAGTGCTCAAAAACGTAATCCATGATGTGGCGACATTATTCCCTGAACCATTTTACCACGGGGGTGCTGATGAGATCGTACCAGGTTGTTGGAAAGCGGATCCAACCATTCAATCATTCTTATCGAGGGGAGGAACTCTGACTTTCCTACCAAAGCTTCCTCCCGCATCTCACCCTGCTCAACGCAGGGAGGAGACTACCGCGGGAAGGAGAAGGAGGAGATCTCGAAGCTAG

Protein Analysis

108

Amino Acids

12.12

Weight (kDa)

9.73

Isoelectric Point (pI)

77.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_20 PF00728 9 - 77 1e-14 Glycosyl hydrolase family 20, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 121
AccII CGCG 2 cut(s) 44, 296
AciI CCGC 4 cut(s) 191, 258, 294, 296
AclWI GGATC 2 cut(s) 188, 201
AcoI YGGCCR 1 cut(s) 11
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 3 cut(s) 59, 121, 300
AgsI TTSAA 2 cut(s) 79, 206
AjnI CCWGG 2 cut(s) 52, 175
AleI CACNNNNGTG 1 cut(s) 156
AluBI AGCT 2 cut(s) 250, 324
AluI AGCT 2 cut(s) 250, 324
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 2 cut(s) 188, 201
AoxI GGCC 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 44, 257
BalI TGGCCA 1 cut(s) 13
BamHI GGATCC 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 102
BciT130I CCWGG 2 cut(s) 54, 177
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 1 cut(s) 325
BglII AGATCT 1 cut(s) 314
Bme1390I CCNGG 2 cut(s) 54, 177
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 2 cut(s) 54, 177
BmsI GCATC 1 cut(s) 269
BoxI GACNNNNGTC 1 cut(s) 43
BplI GAGNNNNNCTC 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 151, 294
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 121, 300
BseBI CCWGG 2 cut(s) 54, 177
BseDI CCNNGG 2 cut(s) 151, 294
BseLI CCNNNNNNNGG 3 cut(s) 59, 121, 300
BseRI GAGGAG 2 cut(s) 299, 325
Bsh1236I CGCG 2 cut(s) 44, 296
BshFI GGCC 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 102
BslI CCNNNNNNNGG 3 cut(s) 59, 121, 300
BsmAI GTCTC 1 cut(s) 281
BsnI GGCC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 3 cut(s) 168, 193, 314
BspACI CCGC 4 cut(s) 191, 258, 294, 296
BspANI GGCC 1 cut(s) 13
BspFNI CGCG 2 cut(s) 44, 296
BspLI GGNNCC 1 cut(s) 195
BspPI GGATC 2 cut(s) 188, 201
BssECI CCNNGG 2 cut(s) 151, 294
BssMI GATC 3 cut(s) 168, 193, 314
Bst2UI CCWGG 2 cut(s) 54, 177
BstC8I GCNNGC 1 cut(s) 15
BstDSI CCRYGG 2 cut(s) 151, 294
BstEII GGTNACC 1 cut(s) 32
BstFNI CGCG 2 cut(s) 44, 296
BstKTI GATC 3 cut(s) 171, 196, 317
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 3 cut(s) 168, 193, 314
BstNI CCWGG 2 cut(s) 54, 177
BstPAI GACNNNNGTC 1 cut(s) 43
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 2 cut(s) 52, 175
BstUI CGCG 2 cut(s) 44, 296
BstX2I RGATCY 2 cut(s) 193, 314
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 2 cut(s) 193, 314
BsuRI GGCC 1 cut(s) 13
BtgI CCRYGG 2 cut(s) 151, 294
Cac8I GCNNGC 1 cut(s) 15
Cfr42I CCGCGG 1 cut(s) 297
CseI GACGC 1 cut(s) 33
CsiI ACCWGGT 1 cut(s) 175
Csp6I GTAC 1 cut(s) 173
CviAII CATG 1 cut(s) 116
CviJI RGCY 3 cut(s) 13, 250, 324
CviKI_1 RGCY 3 cut(s) 13, 250, 324
CviQI GTAC 1 cut(s) 173
DpnI GATC 3 cut(s) 170, 195, 316
DpnII GATC 3 cut(s) 168, 193, 314
EaeI YGGCCR 1 cut(s) 11
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 52, 175
FaeI CATG 1 cut(s) 119
FaiI YATR 1 cut(s) 117
FatI CATG 1 cut(s) 115
FauI CCCGC 2 cut(s) 265, 289
FspBI CTAG 1 cut(s) 325
HaeIII GGCC 1 cut(s) 13
HgaI GACGC 1 cut(s) 33
Hin1II CATG 1 cut(s) 119
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 1 cut(s) 39
HphI GGTGA 2 cut(s) 44, 257
Hpy188I TCNGA 2 cut(s) 49, 234
Hpy188III TCNNGA 1 cut(s) 318
HpyAV CCTTC 2 cut(s) 294, 300
HpyCH4IV ACGT 1 cut(s) 108
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 1 cut(s) 119
KspI CCGCGG 1 cut(s) 297
Kzo9I GATC 3 cut(s) 168, 193, 314
LweI GCATC 1 cut(s) 269
MabI ACCWGGT 1 cut(s) 175
MaeI CTAG 1 cut(s) 325
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 32
MalI GATC 3 cut(s) 170, 195, 316
MboI GATC 3 cut(s) 168, 193, 314
MflI RGATCY 2 cut(s) 193, 314
MhlI GDGCHC 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 13
MluNI TGGCCA 1 cut(s) 13
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 2 cut(s) 164, 221
MnlI CCTC 5 cut(s) 213, 218, 264, 277, 303
Mox20I TGGCCA 1 cut(s) 13
MscI TGGCCA 1 cut(s) 13
MslI CAYNNNNRTG 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 13
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 54, 177
MvaI CCWGG 2 cut(s) 54, 177
MvnI CGCG 2 cut(s) 44, 296
NdeII GATC 3 cut(s) 168, 193, 314
NlaIII CATG 1 cut(s) 119
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 32
OliI CACNNNNGTG 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 121
PleI GAGTC 1 cut(s) 33
PpsI GAGTC 1 cut(s) 33
PshAI GACNNNNGTC 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 52, 175
PspEI GGTNACC 1 cut(s) 32
PspGI CCWGG 2 cut(s) 52, 175
PspN4I GGNNCC 1 cut(s) 195
PsuI RGATCY 2 cut(s) 193, 314
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 156
SacII CCGCGG 1 cut(s) 297
Sau3AI GATC 3 cut(s) 168, 193, 314
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 54, 177
SduI GDGCHC 1 cut(s) 102
SetI ASST 6 cut(s) 64, 92, 111, 181, 252, 326
SexAI ACCWGGT 1 cut(s) 175
SfaNI GCATC 1 cut(s) 269
Sfr303I CCGCGG 1 cut(s) 297
SgrBI CCGCGG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 156
SsiI CCGC 4 cut(s) 191, 258, 294, 296
SspMI CTAG 1 cut(s) 325
StyD4I CCNGG 2 cut(s) 52, 175
TaiI ACGT 1 cut(s) 111
TaqI TCGA 2 cut(s) 218, 319
TaqII GACCGA 1 cut(s) 51
TseFI GTSAC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 32
Van91I CCANNNNNTGG 1 cut(s) 121
XspI CTAG 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.