pycom07g00250

homeobox-leucine zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0018502
Physical Location & Seq
Forward (+)
268336 .. 270086
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGAGCTTTGTTTGAGCTTAGGAGGTCATGAGGAACCTGCAGAGCGATCAGATCAGAACACCACCACCACCACCAAAACGACAGAGTTTTGCATGAGCTTATCAATCGGTCCAAGCTGTGCTGCTGGTACTACTAGTACTCCTCATCCTCCTCCAAGACATGAAGCAGCTGCAGCAGATAAAAAACAAGATGATGATGATGGTGATCATATTCATGTAGATGATCGTGTACCACTTCAACTTGATCTTCTCCTTTGGCCTACTTCTCCTTGTAATCGGATTCAAAACGCTGGTGTGCTGTCCATGAACGTGATCAACAGAGTTGAGGAAGTGGTGGCTGCTGCGTCGTCAACGCACACGAACAGTGGTTCGTCGTCGTTTCAGATCATGGATATCGGCAGTAATAATCACAGCAAGGGCAGTGCAGGTGATGATCTGAATAATAATTACCACAACGTCTACAACTACAAGAGGAGGGCTCACTCTGCAGAAGCAGGAGATGATGAGGGAGAAAGGGCATCTAATTCCAGGGTGGGCAGCGATGAGGAGGAGGACGACATTATCAATGGTAACACTAACACCTCTAGAAAGAAGCTCAGGCTCTCCAAGGAACAATCTGCTTTTCTTGAAGAAAGTTTCAAAGAACACCATACTCTCAATCCTGTAAGTGTAAAGACAAAGTTGAAGCAAACGGAGGTTGACTGTGAGTTCCTGAAGAGATGTTGTGAGACACTGACGGAAGAGAACAGAAGGCTAAACAAAGAGCTACAAGAGTTGAGGGCTTTGAAGGCTACTCATCACTCAAACCCATTCTCCATGCAATCACCAGCCACCACCCTCACCATGTGTCCTTCCTGCGAGCGCACAGCCACCACCGCTAACCCCGCCAGCAAAACCACCAACAATACTAATAACAATAATAATAATAAAGTTGAGTCAACTAGTGCTACAAAAGTCCAAGCAGCTAAATTCCCTCTCAAAAGACCCAGCTTCTATCCATTTTTTCAACCCAAATCCCACCCCTCTTCCGCAGCTTCATGA

Protein Analysis

347

Amino Acids

38.0

Weight (kDa)

5.97

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HALZ PF02183 229 - 263 1.8e-10 Homeobox associated leucine zipper
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 416
Acc36I ACCTGC 2 cut(s) 46, 416
AccI GTMKAC 1 cut(s) 459
AciI CCGC 3 cut(s) 876, 885, 1029
AcsI RAATTY 1 cut(s) 968
AcuI CTGAAG 1 cut(s) 734
AdeI CACNNNGTG 1 cut(s) 846
AfaI GTAC 3 cut(s) 130, 139, 231
AgsI TTSAA 7 cut(s) 239, 284, 629, 640, 685, 787, 1007
AhlI ACTAGT 2 cut(s) 134, 941
AjnI CCWGG 1 cut(s) 527
Alw26I GTCTC 1 cut(s) 722
AoxI GGCC 1 cut(s) 257
ApeKI GCWGC 9 cut(s) 122, 167, 170, 173, 338, 341, 537, 962, 1031
ApoI RAATTY 1 cut(s) 968
AspLEI GCGC 1 cut(s) 864
AspS9I GGNCC 1 cut(s) 110
AsuHPI GGTGA 4 cut(s) 215, 440, 816, 832
AvaII GGWCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 481
BbvI GCAGC 8 cut(s) 109, 157, 179, 185, 325, 328, 549, 974
BccI CCATC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 529
BclI TGATCA 2 cut(s) 205, 312
BcoDI GTCTC 1 cut(s) 722
BcuI ACTAGT 2 cut(s) 134, 941
BfaI CTAG 3 cut(s) 135, 585, 942
BfmI CTRYAG 3 cut(s) 39, 171, 486
BfuAI ACCTGC 2 cut(s) 46, 416
BisI GCNGC 9 cut(s) 123, 168, 171, 174, 339, 342, 538, 963, 1032
BlsI GCNGC 9 cut(s) 124, 169, 172, 175, 340, 343, 539, 964, 1033
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 529
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 529
BmsI GCATC 1 cut(s) 527
BplI GAGNNNNNCTC 2 cut(s) 463, 495
Bpu10I CCTNAGC 2 cut(s) 19, 596
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 528, 606
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 797, 827
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 1 cut(s) 529
BseDI CCNNGG 2 cut(s) 528, 606
BseGI GGATG 1 cut(s) 145
BseJI GATNNNNATC 1 cut(s) 204
BseMII CTCAG 1 cut(s) 610
BseRI GAGGAG 5 cut(s) 132, 141, 487, 560, 563
BseXI GCAGC 8 cut(s) 109, 157, 179, 185, 325, 328, 549, 974
BseYI CCCAGC 1 cut(s) 986
BsgI GTGCAG 1 cut(s) 444
BshFI GGCC 1 cut(s) 259
BsmAI GTCTC 1 cut(s) 722
BsnI GGCC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 481
Bsp143I GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
BspACI CCGC 3 cut(s) 876, 885, 1029
BspANI GGCC 1 cut(s) 259
BspCNI CTCAG 1 cut(s) 609
BspHI TCATGA 2 cut(s) 28, 1037
BspLI GGNNCC 1 cut(s) 36
BspMAI CTGCAG 3 cut(s) 43, 175, 490
BspMI ACCTGC 2 cut(s) 46, 416
BssECI CCNNGG 2 cut(s) 528, 606
BssMI GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 1 cut(s) 529
Bst4CI ACNGT 2 cut(s) 365, 704
Bst6I CTCTTC 3 cut(s) 710, 735, 1030
BstC8I GCNNGC 2 cut(s) 860, 889
BstDEI CTNAG 2 cut(s) 19, 596
BstF5I GGATG 1 cut(s) 145
BstHHI GCGC 1 cut(s) 864
BstKTI GATC 8 cut(s) 50, 55, 208, 226, 247, 315, 387, 436
BstMAI GTCTC 1 cut(s) 722
BstMBI GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
BstMWI GCNNNNNNNGC 5 cut(s) 173, 485, 788, 875, 884
BstNI CCWGG 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 527
BstSFI CTRYAG 3 cut(s) 39, 171, 486
BstV1I GCAGC 8 cut(s) 109, 157, 179, 185, 325, 328, 549, 974
BsuRI GGCC 1 cut(s) 259
BtgZI GCGATG 1 cut(s) 555
BtsCI GGATG 1 cut(s) 145
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 370, 427, 731
BveI ACCTGC 2 cut(s) 46, 416
Cac8I GCNNGC 2 cut(s) 860, 889
CciI TCATGA 2 cut(s) 28, 1037
CfoI GCGC 1 cut(s) 864
Cfr13I GGNCC 1 cut(s) 110
CseI GACGC 1 cut(s) 333
Csp6I GTAC 3 cut(s) 129, 138, 230
CviAII CATG 9 cut(s) 29, 94, 161, 215, 304, 388, 817, 844, 1038
CviQI GTAC 3 cut(s) 129, 138, 230
DdeI CTNAG 2 cut(s) 19, 596
DpnI GATC 8 cut(s) 49, 54, 207, 225, 246, 314, 386, 435
DpnII GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
DraIII CACNNNGTG 1 cut(s) 846
Eam1104I CTCTTC 3 cut(s) 710, 735, 1030
EarI CTCTTC 3 cut(s) 710, 735, 1030
Eco130I CCWWGG 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 481
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 734
EcoRII CCWGG 1 cut(s) 527
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 9 cut(s) 32, 97, 164, 218, 307, 391, 820, 847, 1041
FatI CATG 9 cut(s) 28, 93, 160, 214, 303, 387, 816, 843, 1037
FauI CCCGC 1 cut(s) 892
FbaI TGATCA 2 cut(s) 205, 312
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 9 cut(s) 123, 168, 171, 174, 339, 342, 538, 963, 1032
FokI GGATG 1 cut(s) 132
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 9 cut(s) 123, 168, 171, 174, 339, 342, 538, 963, 1032
FspBI CTAG 3 cut(s) 135, 585, 942
GlaI GCGC 1 cut(s) 863
GluI GCNGC 9 cut(s) 123, 168, 171, 174, 339, 342, 538, 963, 1032
GsaI CCCAGC 1 cut(s) 990
HaeIII GGCC 1 cut(s) 259
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 864
Hin1II CATG 9 cut(s) 32, 97, 164, 218, 307, 391, 820, 847, 1041
Hin6I GCGC 1 cut(s) 862
HinP1I GCGC 1 cut(s) 862
HincII GTYRAC 3 cut(s) 351, 700, 939
HindII GTYRAC 3 cut(s) 351, 700, 939
HinfI GANTC 2 cut(s) 280, 935
HphI GGTGA 4 cut(s) 215, 440, 816, 832
Hpy166II GTNNAC 5 cut(s) 230, 351, 460, 700, 939
Hpy188I TCNGA 5 cut(s) 52, 57, 279, 384, 438
Hpy188III TCNNGA 5 cut(s) 29, 585, 626, 712, 1038
Hpy8I GTNNAC 5 cut(s) 230, 351, 460, 700, 939
Hpy99I CGWCG 3 cut(s) 349, 376, 379
HpyAV CCTTC 3 cut(s) 744, 781, 861
HpyCH4III ACNGT 2 cut(s) 365, 704
HpyCH4IV ACGT 2 cut(s) 309, 456
HpyCH4V TGCA 6 cut(s) 41, 93, 173, 425, 488, 820
HpyF10VI GCNNNNNNNGC 5 cut(s) 173, 485, 788, 875, 884
HpyF3I CTNAG 2 cut(s) 19, 596
HpySE526I ACGT 2 cut(s) 309, 456
Hsp92II CATG 9 cut(s) 32, 97, 164, 218, 307, 391, 820, 847, 1041
HspAI GCGC 1 cut(s) 862
Ksp22I TGATCA 2 cut(s) 205, 312
Kzo9I GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 8 cut(s) 109, 157, 179, 185, 325, 328, 549, 974
LweI GCATC 1 cut(s) 527
MaeI CTAG 3 cut(s) 135, 585, 942
MaeII ACGT 2 cut(s) 309, 456
MaeIII GTNAC 1 cut(s) 569
MalI GATC 8 cut(s) 49, 54, 207, 225, 246, 314, 386, 435
MboI GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
MboII GAAGA 5 cut(s) 239, 641, 727, 752, 1017
MhlI GDGCHC 1 cut(s) 481
MluCI AATT 3 cut(s) 445, 523, 968
MlyI GAGTC 1 cut(s) 944
MslI CAYNNNNRTG 3 cut(s) 213, 219, 308
MspA1I CMGCKG 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 529
MvaI CCWGG 1 cut(s) 529
MwoI GCNNNNNNNGC 5 cut(s) 173, 485, 788, 875, 884
NdeII GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
NlaIII CATG 9 cut(s) 32, 97, 164, 218, 307, 391, 820, 847, 1041
NlaIV GGNNCC 1 cut(s) 36
PagI TCATGA 2 cut(s) 28, 1037
PaqCI CACCTGC 1 cut(s) 416
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 9 cut(s) 124, 169, 172, 175, 340, 343, 539, 964, 1033
PleI GAGTC 1 cut(s) 943
PpsI GAGTC 1 cut(s) 943
Psp6I CCWGG 1 cut(s) 527
PspFI CCCAGC 1 cut(s) 986
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 110
PstI CTGCAG 3 cut(s) 43, 175, 490
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 3 cut(s) 130, 139, 231
RsaNI GTAC 3 cut(s) 129, 138, 230
RseI CAYNNNNRTG 3 cut(s) 213, 219, 308
SatI GCNGC 9 cut(s) 123, 168, 171, 174, 339, 342, 538, 963, 1032
Sau3AI GATC 8 cut(s) 47, 52, 205, 223, 244, 312, 384, 433
Sau96I GGNCC 1 cut(s) 110
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 1 cut(s) 944
ScrFI CCNGG 1 cut(s) 529
SduI GDGCHC 1 cut(s) 481
SfaNI GCATC 1 cut(s) 527
SfcI CTRYAG 3 cut(s) 39, 171, 486
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 3 cut(s) 213, 219, 308
SpeI ACTAGT 2 cut(s) 134, 941
Sse9I AATT 3 cut(s) 445, 523, 968
SsiI CCGC 3 cut(s) 876, 885, 1029
SspMI CTAG 3 cut(s) 135, 585, 942
StyD4I CCNGG 1 cut(s) 527
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 2 cut(s) 365, 704
TaiI ACGT 2 cut(s) 312, 459
TaqII GACCGA 1 cut(s) 98
TasI AATT 3 cut(s) 445, 523, 968
TatI WGTACW 1 cut(s) 137
TfiI GAWTC 1 cut(s) 280
TscAI CASTG 3 cut(s) 370, 427, 738
TseI GCWGC 9 cut(s) 122, 167, 170, 173, 338, 341, 537, 962, 1031
TspDTI ATGAA 4 cut(s) 177, 203, 320, 1026
TspGWI ACGGA 2 cut(s) 707, 752
TspRI CASTG 3 cut(s) 370, 427, 738
VpaK11BI GGWCC 1 cut(s) 110
XapI RAATTY 1 cut(s) 968
XbaI TCTAGA 1 cut(s) 584
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 3 cut(s) 135, 585, 942
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.