pycom07g05220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011464
Physical Location & Seq
Forward (+)
4508214 .. 4510298
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05220.2

Sequence Viewer

Length: 657 bp
ATGCATAATTCTAATCAGGACTCGCAAGATCAGAGGAGTAATTCTGGCATGGAGGATTCCACTGCAATGACAATTGAGTTTCTTCGGGCACGGTTGCTGTCCGAGAGGTCTGTTGCAAGAAGTGCAAGACAGAGAGTTGATGAGCTGGAAAAAATGGTAGAAGAAATGGAGGAACAACTTAAGATTGTATCTCTTCAAAGAAAAATGGCTGAGAAAACCACAGCAGATGTTCTTGCCATTTTGGAGAACCAAGGGGGAAGTGACATTTCCGAGGAATTTGATTCCAGCTCTGATCAGGAAACACACCAGGAATCTAAAGTGGGTAATAACTTGACAAATGAAGAAGAAAGCTTTGTAATTTCGAAGGCGAGAAGAAATGAACAGGAGGAACTTTCGGGTTCTGATGCTGATTCTTGTCTAATACCTGGTAGAAGCTTGTCTTGGAAAGGGCGCATTGAATCTCCACGTTCACGGGAAAAGTATAAAGATCTATCCACAAGAAGACGTAGCTTTTCATCCATGGGTTCTAGTTCTCCAAGACATCACCTTGGAAAATCCTGCCGCCAGATAAAACACAAGGAAACAAGGGTAAAATTGTACTTTCACATGTGCACCTTCTTCGTCCTCGTCTCTCTTTTCTATATCTGTAAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

25.02

Weight (kDa)

6.62

Isoelectric Point (pI)

83.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011464)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52240
fragaria_vesca FvH4_7g05860 FvH4_7g05860
malus_domestica MD02G1248000.v1.1 MD07G1068100.v1.1
prunus_persica Prupe.2G085400_v2.0.a1
pyrus_communis pycom02g21240 pycom07g05220
rosa_chinensis RchiOBHm_Chr1g0333121
rosa_laevigata RLG00000029625
rosa_multiflora Rmu_sc0007432.1_g000002
rosa_roxburghii Rroxscaffold_4G00317780
rosa_rugosa Rorug01G0107100 Rorug01G0107100 Rorug01G0107200
rosa_samantha Rh1AG130500 Rh1BG099600 Rh1CG124300 Rh1DG135800
rosa_wichuraiana Rw1G010250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 562
AcsI RAATTY 1 cut(s) 275
AfaI GTAC 1 cut(s) 599
AflII CTTAAG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 2 cut(s) 197, 458
AjnI CCWGG 2 cut(s) 306, 424
AjuI GAANNNNNNNTTGG 2 cut(s) 424, 456
AluBI AGCT 5 cut(s) 145, 288, 351, 435, 510
AluI AGCT 5 cut(s) 145, 288, 351, 435, 510
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 1 cut(s) 634
Alw44I GTGCAC 1 cut(s) 610
ApaLI GTGCAC 1 cut(s) 610
ApoI RAATTY 1 cut(s) 275
AspLEI GCGC 1 cut(s) 453
AsuHPI GGTGA 1 cut(s) 536
AsuII TTCGAA 1 cut(s) 362
BaeGI GKGCMC 2 cut(s) 91, 614
BbsI GAAGAC 1 cut(s) 508
Bbv12I GWGCWC 1 cut(s) 614
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 2 cut(s) 308, 426
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 179
BglII AGATCT 1 cut(s) 487
BisI GCNGC 1 cut(s) 562
BlsI GCNGC 1 cut(s) 563
Bme1390I CCNGG 2 cut(s) 308, 426
BmrFI CCNGG 2 cut(s) 308, 426
BmsI GCATC 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 508
Bpu14I TTCGAA 1 cut(s) 362
BsaJI CCNNGG 4 cut(s) 250, 270, 519, 547
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 2 cut(s) 308, 426
BseDI CCNNGG 4 cut(s) 250, 270, 519, 547
BseGI GGATG 1 cut(s) 515
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 201
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 2 cut(s) 91, 614
BsiHKAI GWGCWC 1 cut(s) 614
BsmAI GTCTC 1 cut(s) 634
BsmBI CGTCTC 1 cut(s) 634
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 91, 614
Bsp143I GATC 3 cut(s) 28, 292, 487
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 1 cut(s) 562
BspCNI CTCAG 1 cut(s) 202
BspT104I TTCGAA 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 72
BssECI CCNNGG 4 cut(s) 250, 270, 519, 547
BssMI GATC 3 cut(s) 28, 292, 487
BssT1I CCWWGG 3 cut(s) 250, 519, 547
Bst2UI CCWGG 2 cut(s) 308, 426
Bst4CI ACNGT 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 198
BstAFI CTTAAG 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 122
BstBI TTCGAA 1 cut(s) 362
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 515
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 3 cut(s) 31, 295, 490
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 3 cut(s) 28, 292, 487
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstNI CCWGG 2 cut(s) 308, 426
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 306, 424
BstSLI GKGCMC 2 cut(s) 91, 614
BstV2I GAAGAC 1 cut(s) 508
BstX2I RGATCY 1 cut(s) 487
BstYI RGATCY 1 cut(s) 487
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 1 cut(s) 515
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 60
CfoI GCGC 1 cut(s) 453
CsiI ACCWGGT 1 cut(s) 424
Csp6I GTAC 1 cut(s) 598
CviAII CATG 3 cut(s) 49, 520, 607
CviJI RGCY 6 cut(s) 145, 209, 288, 351, 435, 510
CviKI_1 RGCY 6 cut(s) 145, 209, 288, 351, 435, 510
CviQI GTAC 1 cut(s) 598
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 30, 294, 489
DpnII GATC 3 cut(s) 28, 292, 487
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 3 cut(s) 250, 519, 547
EcoRII CCWGG 2 cut(s) 306, 424
EcoT14I CCWWGG 3 cut(s) 250, 519, 547
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 250, 519, 547
Esp3I CGTCTC 1 cut(s) 634
FaeI CATG 3 cut(s) 52, 523, 610
FaiI YATR 6 cut(s) 6, 50, 483, 521, 608, 642
FalI AAGNNNNNCTT 2 cut(s) 424, 456
FatI CATG 3 cut(s) 48, 519, 606
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 1 cut(s) 562
FokI GGATG 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 562
FspBI CTAG 1 cut(s) 528
GlaI GCGC 1 cut(s) 452
GluI GCNGC 1 cut(s) 562
HhaI GCGC 1 cut(s) 453
Hin1II CATG 3 cut(s) 52, 523, 610
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HindIII AAGCTT 2 cut(s) 349, 433
HinfI GANTC 6 cut(s) 20, 56, 281, 311, 410, 458
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 2 cut(s) 470, 612
Hpy188I TCNGA 6 cut(s) 33, 103, 271, 292, 403, 656
Hpy188III TCNNGA 2 cut(s) 17, 296
Hpy8I GTNNAC 2 cut(s) 470, 612
HpyAV CCTTC 2 cut(s) 358, 625
HpyCH4III ACNGT 1 cut(s) 93
HpyCH4IV ACGT 2 cut(s) 466, 505
HpyCH4V TGCA 5 cut(s) 4, 65, 116, 125, 612
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 1 cut(s) 210
HpySE526I ACGT 2 cut(s) 466, 505
Hsp92II CATG 3 cut(s) 52, 523, 610
HspAI GCGC 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 3 cut(s) 28, 292, 487
LweI GCATC 1 cut(s) 394
MabI ACCWGGT 1 cut(s) 424
MaeI CTAG 1 cut(s) 528
MaeII ACGT 2 cut(s) 466, 505
MaeIII GTNAC 1 cut(s) 260
MalI GATC 3 cut(s) 30, 294, 489
MboI GATC 3 cut(s) 28, 292, 487
MboII GAAGA 8 cut(s) 74, 173, 185, 353, 356, 384, 513, 610
MfeI CAATTG 1 cut(s) 72
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 2 cut(s) 91, 614
MluCI AATT 6 cut(s) 7, 40, 72, 275, 357, 593
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 7 cut(s) 27, 46, 99, 163, 265, 379, 635
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 180
MslI CAYNNNNRTG 1 cut(s) 65
MspCI CTTAAG 1 cut(s) 179
MspR9I CCNGG 2 cut(s) 308, 426
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 2 cut(s) 308, 426
MwoI GCNNNNNNNGC 1 cut(s) 122
NcoI CCATGG 1 cut(s) 519
NdeII GATC 3 cut(s) 28, 292, 487
NlaIII CATG 3 cut(s) 52, 523, 610
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 610
NspV TTCGAA 1 cut(s) 362
PciI ACATGT 1 cut(s) 606
PfeI GAWTC 5 cut(s) 56, 281, 311, 410, 458
PkrI GCNGC 1 cut(s) 563
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PscI ACATGT 1 cut(s) 606
Psp6I CCWGG 2 cut(s) 306, 424
PspGI CCWGG 2 cut(s) 306, 424
PsuI RGATCY 1 cut(s) 487
RsaI GTAC 1 cut(s) 599
RsaNI GTAC 1 cut(s) 598
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 1 cut(s) 562
Sau3AI GATC 3 cut(s) 28, 292, 487
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 308, 426
SduI GDGCHC 2 cut(s) 91, 614
SexAI ACCWGGT 1 cut(s) 424
SfaNI GCATC 1 cut(s) 394
SfuI TTCGAA 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 6 cut(s) 7, 40, 72, 275, 357, 593
SsiI CCGC 1 cut(s) 562
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 2 cut(s) 306, 424
StyI CCWWGG 3 cut(s) 250, 519, 547
TaaI ACNGT 1 cut(s) 93
TaiI ACGT 2 cut(s) 469, 508
TaqI TCGA 1 cut(s) 362
TasI AATT 6 cut(s) 7, 40, 72, 275, 357, 593
TatI WGTACW 1 cut(s) 597
TauI GCSGC 1 cut(s) 564
TfiI GAWTC 5 cut(s) 56, 281, 311, 410, 458
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 3 cut(s) 354, 393, 504
TspRI CASTG 1 cut(s) 67
Vha464I CTTAAG 1 cut(s) 179
VneI GTGCAC 1 cut(s) 610
XapI RAATTY 1 cut(s) 275
XceI RCATGY 1 cut(s) 610
XspI CTAG 1 cut(s) 528
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.