pycom07g10490

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0019318
Physical Location & Seq
Forward (+)
10526648 .. 10529080
2433 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 417 bp
ATGTTCACCTCCCGCACCACACACCACTTTAGGTGGTTCCGACTCGTAGGTGACCCGCGATTATTCGCACAGCCTTCACGTGATCGTAGCACTAGAGCGTATATGTATATTACACCCAGGCCTGTCGTACAGACCACTTTAGGCCGTATCCTCCAGCTGCAGCCGGCGGTTCTCAGTGGTACCAGGGATGACAGTATCAGCAGGGAGAGATTGCCACTAGTAGTGCAGGGTCGTCACGACAGTCGGGTCAGCCCAGTCAGGGGCGTGGTGCTCAGGGACGCGGTGTTCGGGCGAGCGGAGGATGCCCAAAACAACCCAGACTTGATTATGGGTACGTTAAACATTCTTGGTTGTTTTGCTAGAGTCTTAATTGATTGTGGAGCTACACATTCCGTGATTTCTCATACATTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

139

Amino Acids

15.53

Weight (kDa)

11.13

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVP_2 PF08284 98 - 138 6.1e-09 Retroviral aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AccBSI CCGCTC 1 cut(s) 296
AccII CGCG 2 cut(s) 58, 281
AciI CCGC 5 cut(s) 13, 56, 167, 281, 296
AcvI CACGTG 1 cut(s) 80
AfaI GTAC 3 cut(s) 129, 181, 334
AfiI CCNNNNNNNGG 2 cut(s) 259, 260
AhlI ACTAGT 1 cut(s) 217
AjnI CCWGG 2 cut(s) 116, 182
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 2 cut(s) 157, 383
AluI AGCT 2 cut(s) 157, 383
Alw21I GWGCWC 1 cut(s) 273
AoxI GGCC 2 cut(s) 119, 142
ApeKI GCWGC 2 cut(s) 157, 160
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
Asp718I GGTACC 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 62
BanI GGYRCC 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 144, 172
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 2 cut(s) 118, 184
BciVI GTATCC 1 cut(s) 158
BcuI ACTAGT 1 cut(s) 217
BfaI CTAG 3 cut(s) 93, 218, 360
BfmI CTRYAG 1 cut(s) 158
BfuI GTATCC 1 cut(s) 158
BisI GCNGC 2 cut(s) 158, 161
BlsI GCNGC 2 cut(s) 159, 162
Bme1390I CCNGG 2 cut(s) 118, 184
BmiI GGNNCC 2 cut(s) 38, 181
BmrFI CCNGG 2 cut(s) 118, 184
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 1 cut(s) 292
BmuI ACTGGG 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 116, 183
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 260
Bse118I RCCGGY 1 cut(s) 163
Bse1I ACTGG 1 cut(s) 254
BseBI CCWGG 2 cut(s) 118, 184
BseDI CCNNGG 2 cut(s) 116, 183
BseGI GGATG 2 cut(s) 193, 307
BseLI CCNNNNNNNGG 2 cut(s) 259, 260
BseMII CTCAG 2 cut(s) 187, 286
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 2 cut(s) 144, 172
BsgI GTGCAG 1 cut(s) 245
Bsh1236I CGCG 2 cut(s) 58, 281
BshFI GGCC 2 cut(s) 121, 144
BshNI GGYRCC 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 164
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 2 cut(s) 259, 260
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 2 cut(s) 121, 144
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 82
BspACI CCGC 5 cut(s) 13, 56, 167, 281, 296
BspANI GGCC 2 cut(s) 121, 144
BspCNI CTCAG 2 cut(s) 186, 285
BspFNI CGCG 2 cut(s) 58, 281
BspLI GGNNCC 2 cut(s) 38, 181
BspMAI CTGCAG 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 179
BsrBI CCGCTC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 163
BsrI ACTGG 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 2 cut(s) 116, 183
BssMI GATC 1 cut(s) 82
Bst2UI CCWGG 2 cut(s) 118, 184
Bst4CI ACNGT 2 cut(s) 194, 242
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 165, 294
BstDEI CTNAG 2 cut(s) 173, 272
BstEII GGTNACC 1 cut(s) 50
BstF5I GGATG 2 cut(s) 193, 307
BstFNI CGCG 2 cut(s) 58, 281
BstKTI GATC 1 cut(s) 85
BstMBI GATC 1 cut(s) 82
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 2 cut(s) 118, 184
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 2 cut(s) 116, 182
BstSFI CTRYAG 1 cut(s) 158
BstUI CGCG 2 cut(s) 58, 281
BstV1I GCAGC 2 cut(s) 144, 172
BsuI GTATCC 1 cut(s) 158
BsuRI GGCC 2 cut(s) 121, 144
BtsCI GGATG 2 cut(s) 193, 307
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 2 cut(s) 165, 294
Cfr10I RCCGGY 1 cut(s) 163
CseI GACGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 128, 180, 333
CviJI RGCY 7 cut(s) 73, 121, 144, 157, 163, 252, 383
CviKI_1 RGCY 7 cut(s) 73, 121, 144, 157, 163, 252, 383
CviQI GTAC 3 cut(s) 128, 180, 333
DdeI CTNAG 2 cut(s) 173, 272
DpnI GATC 1 cut(s) 84
DpnII GATC 1 cut(s) 82
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Eco147I AGGCCT 1 cut(s) 121
Eco72I CACGTG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 50
EcoRII CCWGG 2 cut(s) 116, 182
FaiI YATR 5 cut(s) 102, 104, 108, 329, 405
FaqI GGGAC 1 cut(s) 290
FauI CCCGC 2 cut(s) 20, 63
Fnu4HI GCNGC 2 cut(s) 158, 161
FokI GGATG 2 cut(s) 200, 314
Fsp4HI GCNGC 2 cut(s) 158, 161
FspBI CTAG 3 cut(s) 93, 218, 360
GluI GCNGC 2 cut(s) 158, 161
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 2 cut(s) 121, 144
HapII CCGG 1 cut(s) 164
HgaI GACGC 1 cut(s) 287
HinfI GANTC 2 cut(s) 42, 363
HpaII CCGG 1 cut(s) 164
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 1 cut(s) 84
HpyCH4III ACNGT 2 cut(s) 194, 242
HpyCH4IV ACGT 2 cut(s) 79, 335
HpyCH4V TGCA 2 cut(s) 160, 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 2 cut(s) 173, 272
HpySE526I ACGT 2 cut(s) 79, 335
KpnI GGTACC 1 cut(s) 183
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Kzo9I GATC 1 cut(s) 82
LmnI GCTCC 1 cut(s) 380
Lsp1109I GCAGC 2 cut(s) 144, 172
LweI GCATC 1 cut(s) 292
MaeI CTAG 3 cut(s) 93, 218, 360
MaeII ACGT 2 cut(s) 79, 335
MaeIII GTNAC 2 cut(s) 50, 233
MalI GATC 1 cut(s) 84
MbiI CCGCTC 1 cut(s) 296
MboI GATC 1 cut(s) 82
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 1 cut(s) 369
MlyI GAGTC 2 cut(s) 36, 372
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 3 cut(s) 19, 161, 292
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 3 cut(s) 338, 368, 415
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 118, 184
MvaI CCWGG 2 cut(s) 118, 184
MvnI CGCG 2 cut(s) 58, 281
MwoI GCNNNNNNNGC 1 cut(s) 302
NaeI GCCGGC 1 cut(s) 165
NdeII GATC 1 cut(s) 82
NgoMIV GCCGGC 1 cut(s) 163
NlaIV GGNNCC 2 cut(s) 38, 181
NmuCI GTSAC 2 cut(s) 50, 233
PceI AGGCCT 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 165
PkrI GCNGC 2 cut(s) 159, 162
PleI GAGTC 2 cut(s) 36, 371
PmaCI CACGTG 1 cut(s) 80
PmlI CACGTG 1 cut(s) 80
PpsI GAGTC 2 cut(s) 36, 371
Ppu21I YACGTR 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 116, 182
PspCI CACGTG 1 cut(s) 80
PspEI GGTNACC 1 cut(s) 50
PspGI CCWGG 2 cut(s) 116, 182
PspN4I GGNNCC 2 cut(s) 38, 181
PstI CTGCAG 1 cut(s) 162
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 3 cut(s) 129, 181, 334
RsaNI GTAC 3 cut(s) 128, 180, 333
SaqAI TTAA 3 cut(s) 338, 368, 415
SatI GCNGC 2 cut(s) 158, 161
Sau3AI GATC 1 cut(s) 82
SchI GAGTC 2 cut(s) 36, 372
ScrFI CCNGG 2 cut(s) 118, 184
SduI GDGCHC 1 cut(s) 273
SetI ASST 7 cut(s) 11, 35, 52, 82, 159, 338, 385
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 217
Sse9I AATT 1 cut(s) 369
SseBI AGGCCT 1 cut(s) 121
SsiI CCGC 5 cut(s) 13, 56, 167, 281, 296
SspMI CTAG 3 cut(s) 93, 218, 360
StuI AGGCCT 1 cut(s) 121
StyD4I CCNGG 2 cut(s) 116, 182
TaaI ACNGT 2 cut(s) 194, 242
TaiI ACGT 2 cut(s) 82, 338
TasI AATT 1 cut(s) 369
Tru1I TTAA 3 cut(s) 338, 368, 415
Tru9I TTAA 3 cut(s) 338, 368, 415
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 2 cut(s) 50, 233
TseI GCWGC 2 cut(s) 157, 160
Tsp45I GTSAC 2 cut(s) 50, 233
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 1 cut(s) 181
XspI CTAG 3 cut(s) 93, 218, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.