pycom14g06850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019318
Physical Location & Seq
Reverse (-)
6806135 .. 6806689
555 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06850.2

Sequence Viewer

Length: 375 bp
ATGACAGAGGGAAAAGTCAAGTGTCGCTTGGGCCTCGCCAAACTCAGAACTTTAAAAGGGGTGGTGCCAGTTCGAGTTCGTCTAGCGGTGGTTTCAGTGCCTTTGGACAAGGACGTGGAGGTAGGTTTTATGGAAGTGCTCGAGGCCAGAGACAAGGTGATGGTGGCCGAAACCGAATCCCATTTTGCCGTAGGTGTAGAGGTGGTGCCTATCACTATCAGGGTGATGTTGTTCCCTATGCTCCGGGACCGTATCAGTACCCCCAGGACCCGTATTCACAAAGTGGTTATCCCCCGTATCCCAGCAACTACATGCCGTATCCTCCAGCTCCAACGAGTGGTTCTCAGTGGTACCAAGGAGGACAGTATCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.84

Weight (kDa)

10.2

Isoelectric Point (pI)

27.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 350
AccB1I GGYRCC 3 cut(s) 64, 205, 350
AccB7I CCANNNNNTGG 1 cut(s) 337
AciI CCGC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 283
AfaI GTAC 2 cut(s) 259, 352
AfiI CCNNNNNNNGG 1 cut(s) 337
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 1 cut(s) 263
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AluBI AGCT 2 cut(s) 328, 372
AluI AGCT 2 cut(s) 328, 372
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 3 cut(s) 31, 144, 165
Asp718I GGTACC 1 cut(s) 350
AspS9I GGNCC 3 cut(s) 31, 247, 267
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 169, 235
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 2 cut(s) 247, 267
BanI GGYRCC 3 cut(s) 64, 205, 350
Bbv12I GWGCWC 1 cut(s) 141
BccI CCATC 1 cut(s) 154
BceAI ACGGC 2 cut(s) 173, 300
BciT130I CCWGG 1 cut(s) 265
BciVI GTATCC 2 cut(s) 308, 329
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 1 cut(s) 83
BfuI GTATCC 2 cut(s) 308, 329
Bme1390I CCNGG 2 cut(s) 245, 265
Bme18I GGWCC 2 cut(s) 247, 267
BmeT110I CYCGRG 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 3 cut(s) 31, 247, 267
BmiI GGNNCC 5 cut(s) 66, 207, 248, 269, 352
BmrFI CCNGG 2 cut(s) 245, 265
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpmI CTGGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 263, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 68
BseBI CCWGG 1 cut(s) 265
BseDI CCNNGG 2 cut(s) 263, 354
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMII CTCAG 2 cut(s) 58, 358
BseNI ACTGG 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 301
BshFI GGCC 3 cut(s) 33, 146, 167
BshNI GGYRCC 3 cut(s) 64, 205, 350
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 3 cut(s) 33, 146, 167
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 141
BspACI CCGC 1 cut(s) 86
BspANI GGCC 3 cut(s) 33, 146, 167
BspCNI CTCAG 2 cut(s) 57, 357
BspLI GGNNCC 5 cut(s) 66, 207, 248, 269, 352
BspT107I GGYRCC 3 cut(s) 64, 205, 350
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 263, 354
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 265
Bst4CI ACNGT 2 cut(s) 251, 365
BstDEI CTNAG 2 cut(s) 44, 344
BstMAI GTCTC 1 cut(s) 144
BstNI CCWGG 1 cut(s) 265
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 2 cut(s) 243, 263
BsuI GTATCC 2 cut(s) 308, 329
BsuRI GGCC 3 cut(s) 33, 146, 167
BtrI CACGTC 1 cut(s) 115
BtsIMutI CAGTG 2 cut(s) 102, 352
Cfr13I GGNCC 3 cut(s) 31, 247, 267
Csp6I GTAC 2 cut(s) 258, 351
CviAII CATG 1 cut(s) 312
CviJI RGCY 5 cut(s) 33, 146, 167, 328, 372
CviKI_1 RGCY 5 cut(s) 33, 146, 167, 328, 372
CviQI GTAC 2 cut(s) 258, 351
DdeI CTNAG 2 cut(s) 44, 344
DraI TTTAAA 1 cut(s) 54
DraIII CACNNNGTG 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 247, 267
Eco88I CYCGRG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 1 cut(s) 315
FaiI YATR 3 cut(s) 131, 239, 313
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 260
FatI CATG 1 cut(s) 311
FspBI CTAG 1 cut(s) 83
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 3 cut(s) 33, 146, 167
HapII CCGG 1 cut(s) 244
Hin1II CATG 1 cut(s) 315
HinfI GANTC 1 cut(s) 176
HpaII CCGG 1 cut(s) 244
HphI GGTGA 2 cut(s) 169, 235
Hpy188I TCNGA 1 cut(s) 47
HpyCH4III ACNGT 2 cut(s) 251, 365
HpyCH4IV ACGT 1 cut(s) 114
HpyF3I CTNAG 2 cut(s) 44, 344
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 1 cut(s) 315
KpnI GGTACC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 246, 333
LpnPI CCDG 8 cut(s) 81, 160, 205, 250, 257, 277, 315, 338
MaeI CTAG 1 cut(s) 83
MaeII ACGT 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 141
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 6 cut(s) 44, 112, 136, 193, 332, 352
MseI TTAA 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 2 cut(s) 245, 265
MvaI CCWGG 1 cut(s) 265
NciI CCSGG 1 cut(s) 245
NlaIII CATG 1 cut(s) 315
NlaIV GGNNCC 5 cut(s) 66, 207, 248, 269, 352
NspI RCATGY 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 140
PfeI GAWTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 337
PfoI TCCNGGA 1 cut(s) 243
PpuMI RGGWCCY 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 263
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 1 cut(s) 263
PspN4I GGNNCC 5 cut(s) 66, 207, 248, 269, 352
PspPI GGNCC 3 cut(s) 31, 247, 267
PspPPI RGGWCCY 1 cut(s) 267
PspXI VCTCGAGB 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 372
RsaI GTAC 2 cut(s) 259, 352
RsaNI GTAC 2 cut(s) 258, 351
SaqAI TTAA 1 cut(s) 53
Sau96I GGNCC 3 cut(s) 31, 247, 267
ScrFI CCNGG 2 cut(s) 245, 265
SduI GDGCHC 1 cut(s) 141
SetI ASST 8 cut(s) 117, 123, 127, 159, 196, 204, 330, 374
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 2 cut(s) 247, 267
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
SsiI CCGC 1 cut(s) 86
SspMI CTAG 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 243, 263
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 251, 365
TaiI ACGT 1 cut(s) 117
TaqI TCGA 2 cut(s) 73, 141
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 2 cut(s) 102, 352
TspRI CASTG 2 cut(s) 102, 352
Van91I CCANNNNNTGG 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 247, 267
XceI RCATGY 1 cut(s) 315
XhoI CTCGAG 1 cut(s) 140
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.