pycom07g12140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0020369
Physical Location & Seq
Forward (+)
13444225 .. 13445927
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12140.12

Sequence Viewer

Length: 1155 bp
ATGGTCGACGGTAGGGTCGACAACCCTATACTAAAATTTCATTACGATCCAATGGTTGGATCTCCGCTAATTGCAAATTCAATCGGAAAGATCTATAGGTTGGATTCCCGATCCATAAGTTCCTATGGTCCTCAAATATTACCATCAATTACTATTCTAATCAAGTGGCGGACCTTGATGGAACTTCGTTGGATTCTGCAGATTTTGCAGATTTCCTGGGCAATATTTAGAGCTACCAAATCGAAGCTAGACAAGTGTAGAACAAAATTATACTGCCTGGAGTACAAATGGTCGCCGGAAATGGCCATAAAATGGCCTGAAAGACGGCTGTCCGAGGGAAAACATGGAAATTCGCCAAAATTTCTGGGCAACCTTTCAATGTCCATAACTTTCATAGTTCTCGATGAGACGAAGAGATTGGTACCTTTCACAATGGCTATCTCGCCGTGGTTTGGCCGAAAAACGCCACGAAAATGGCCGAGGTCGCCAGAAACTAGGTTAACTTTAAATCATTATAACTTTCTCATTGAGACAAATCAAATTATACCGTATAGTGTTCAAAATATGGCTAGAAATTATCTGAATCACGGCGGTGAAGACATGCAGATAATGGTGGTTTTGATGTTCAACAAGCCGAAAGATTTGGTGATGATGGTGGTTTGTTTCATGCGAGAAGGGAAGAGCCGAGAGAGAGTTAGAGAGTTAGAGAGAGTGAAGAGTTGCAGGAAAGAAGAGGAAGGAGAGAAGGTTCCAGATATTTTGGGGACCAATCTATGGGTCCCACATGTCACACTATTCCAGATACAACATGTATTTCAAATGGAAAATTACCAAAATGCCCTCGATTCTGTTTGCACCCACGTGTTCATAACAACGAGTACTACGAGAATACGCCAAGAAAATGAGTCTTACATGGCACAACACATCAGTCAACAAGAGTCAACACTTTACCTTGAAGGGCATTTTTTGACGGGTCATATACCCCCCCTTAAGAAAATTTCGTCCTCAAAATTTAAAATAACTTCAAATTTAAAGTTCAAGCTATCTAGATTGATTGCCAGCCATTGCACTACAAAAACAACTTCCGGAATAAAGGTGATTGAAGAATTAATGATCACAACCAGATTTCAATATCCAATCAATTCTTCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

385

Amino Acids

44.78

Weight (kDa)

9.63

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020369)

Species Orthologous Gene IDs
malus_domestica MD03G1116600.v1.1
pyrus_communis pycom07g12140 pycom08g18400 pycom13g27360 pycom13g27370 pycom17g18000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 516
AasI GACNNNNNNGTC 1 cut(s) 14
Acc65I GGTACC 1 cut(s) 421
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 3 cut(s) 56, 312, 774
AccI GTMKAC 2 cut(s) 6, 18
AccIII TCCGGA 1 cut(s) 1085
AciI CCGC 3 cut(s) 65, 169, 591
AclWI GGATC 3 cut(s) 41, 67, 105
AcoI YGGCCR 3 cut(s) 303, 454, 476
AcsI RAATTY 7 cut(s) 35, 76, 349, 359, 996, 1010, 1027
AcvI CACGTG 1 cut(s) 862
AfaI GTAC 3 cut(s) 284, 423, 880
AfiI CCNNNNNNNGG 4 cut(s) 56, 312, 452, 774
AflII CTTAAG 1 cut(s) 989
AflIII ACRYGT 3 cut(s) 784, 808, 861
AjnI CCWGG 2 cut(s) 215, 276
AjuI GAANNNNNNNTTGG 1 cut(s) 1129
AleI CACNNNNGTG 2 cut(s) 591, 860
AloI GAACNNNNNNTCC 2 cut(s) 732, 764
AluBI AGCT 3 cut(s) 233, 247, 1042
AluI AGCT 3 cut(s) 233, 247, 1042
Alw26I GTCTC 2 cut(s) 401, 524
AlwI GGATC 3 cut(s) 41, 67, 105
Aor13HI TCCGGA 1 cut(s) 1085
AoxI GGCC 4 cut(s) 303, 314, 454, 476
ApoI RAATTY 7 cut(s) 35, 76, 349, 359, 996, 1010, 1027
AseI ATTAAT 1 cut(s) 1109
Asp718I GGTACC 1 cut(s) 421
AspS9I GGNCC 4 cut(s) 128, 171, 765, 778
AsuHPI GGTGA 3 cut(s) 605, 658, 1108
AvaII GGWCC 4 cut(s) 128, 171, 765, 778
BalI TGGCCA 1 cut(s) 305
BanI GGYRCC 1 cut(s) 421
BbrPI CACGTG 1 cut(s) 862
BbsI GAAGAC 1 cut(s) 603
BccI CCATC 3 cut(s) 151, 172, 646
BceAI ACGGC 3 cut(s) 341, 430, 604
BciT130I CCWGG 2 cut(s) 217, 278
BclI TGATCA 1 cut(s) 1113
BcoDI GTCTC 2 cut(s) 401, 524
BfaI CTAG 4 cut(s) 248, 495, 570, 1047
BfmI CTRYAG 2 cut(s) 94, 197
BfrI CTTAAG 1 cut(s) 989
BglII AGATCT 1 cut(s) 90
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 2 cut(s) 217, 278
Bme18I GGWCC 4 cut(s) 128, 171, 765, 778
BmgT120I GGNCC 4 cut(s) 128, 171, 765, 778
BmiI GGNNCC 5 cut(s) 423, 750, 766, 779, 780
BmrFI CCNGG 2 cut(s) 217, 278
BoxI GACNNNNGTC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 862
BsaJI CCNNGG 4 cut(s) 216, 333, 446, 479
BsaWI WCCGGW 1 cut(s) 1085
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 312, 452, 774
Bse3DI GCAATG 1 cut(s) 1063
BseAI TCCGGA 1 cut(s) 1085
BseBI CCWGG 2 cut(s) 217, 278
BseDI CCNNGG 4 cut(s) 216, 333, 446, 479
BseLI CCNNNNNNNGG 4 cut(s) 56, 312, 452, 774
BseMI GCAATG 1 cut(s) 1063
BshFI GGCC 4 cut(s) 305, 316, 456, 478
BshNI GGYRCC 1 cut(s) 421
BsiSI CCGG 2 cut(s) 296, 1086
BslFI GGGAC 2 cut(s) 764, 778
BslI CCNNNNNNNGG 4 cut(s) 56, 312, 452, 774
BsmAI GTCTC 2 cut(s) 401, 524
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 2 cut(s) 764, 778
BsnI GGCC 4 cut(s) 305, 316, 456, 478
Bsp13I TCCGGA 1 cut(s) 1085
Bsp143I GATC 5 cut(s) 46, 59, 90, 110, 1113
BspACI CCGC 3 cut(s) 65, 169, 591
BspANI GGCC 4 cut(s) 305, 316, 456, 478
BspEI TCCGGA 1 cut(s) 1085
BspLI GGNNCC 5 cut(s) 423, 750, 766, 779, 780
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 3 cut(s) 41, 67, 105
BspQI GCTCTTC 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 421
BspTI CTTAAG 1 cut(s) 989
BsrDI GCAATG 1 cut(s) 1063
BssECI CCNNGG 4 cut(s) 216, 333, 446, 479
BssMI GATC 5 cut(s) 46, 59, 90, 110, 1113
Bst2UI CCWGG 2 cut(s) 217, 278
Bst4CI ACNGT 2 cut(s) 11, 549
Bst6I CTCTTC 4 cut(s) 407, 674, 710, 726
BstAFI CTTAAG 1 cut(s) 989
BstAPI GCANNNNNTGC 1 cut(s) 205
BstBAI YACGTR 1 cut(s) 862
BstC8I GCNNGC 1 cut(s) 1060
BstDSI CCRYGG 1 cut(s) 446
BstKTI GATC 5 cut(s) 49, 62, 93, 113, 1116
BstMAI GTCTC 2 cut(s) 401, 524
BstMBI GATC 5 cut(s) 46, 59, 90, 110, 1113
BstMWI GCNNNNNNNGC 2 cut(s) 205, 484
BstNI CCWGG 2 cut(s) 217, 278
BstNSI RCATGY 3 cut(s) 604, 788, 812
BstPAI GACNNNNGTC 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 215, 276
BstSFI CTRYAG 2 cut(s) 94, 197
BstV2I GAAGAC 1 cut(s) 603
BstX2I RGATCY 2 cut(s) 59, 90
BstXI CCANNNNNNTGG 1 cut(s) 474
BstYI RGATCY 2 cut(s) 59, 90
BsuRI GGCC 4 cut(s) 305, 316, 456, 478
BtgI CCRYGG 1 cut(s) 446
Cac8I GCNNGC 1 cut(s) 1060
Cfr13I GGNCC 4 cut(s) 128, 171, 765, 778
Csp6I GTAC 3 cut(s) 283, 422, 879
CviAII CATG 6 cut(s) 344, 601, 667, 785, 809, 913
CviQI GTAC 3 cut(s) 283, 422, 879
DpnI GATC 5 cut(s) 48, 61, 92, 112, 1115
DpnII GATC 5 cut(s) 46, 59, 90, 110, 1113
DraI TTTAAA 3 cut(s) 507, 1015, 1032
DrdI GACNNNNNNGTC 1 cut(s) 14
DseDI GACNNNNNNGTC 1 cut(s) 14
EaeI YGGCCR 3 cut(s) 303, 454, 476
Eam1104I CTCTTC 4 cut(s) 407, 674, 710, 726
EarI CTCTTC 4 cut(s) 407, 674, 710, 726
EciI GGCGGA 1 cut(s) 184
Eco47I GGWCC 4 cut(s) 128, 171, 765, 778
Eco72I CACGTG 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 215, 276
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 6 cut(s) 347, 604, 670, 788, 812, 916
FaqI GGGAC 2 cut(s) 764, 778
FatI CATG 6 cut(s) 343, 600, 666, 784, 808, 912
FbaI TGATCA 1 cut(s) 1113
FblI GTMKAC 2 cut(s) 6, 18
FspBI CTAG 4 cut(s) 248, 495, 570, 1047
GsuI CTGGAG 1 cut(s) 299
HaeIII GGCC 4 cut(s) 305, 316, 456, 478
HapII CCGG 2 cut(s) 296, 1086
Hin1II CATG 6 cut(s) 347, 604, 670, 788, 812, 916
HincII GTYRAC 5 cut(s) 7, 19, 501, 932, 942
HindII GTYRAC 5 cut(s) 7, 19, 501, 932, 942
HinfI GANTC 6 cut(s) 104, 193, 583, 845, 905, 938
HpaI GTTAAC 1 cut(s) 501
HpaII CCGG 2 cut(s) 296, 1086
HphI GGTGA 3 cut(s) 605, 658, 1108
Hpy166II GTNNAC 5 cut(s) 7, 19, 501, 932, 942
Hpy188I TCNGA 4 cut(s) 86, 334, 582, 1150
Hpy188III TCNNGA 6 cut(s) 108, 401, 752, 799, 1047, 1086
Hpy8I GTNNAC 5 cut(s) 7, 19, 501, 932, 942
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 668, 731, 739, 950
HpyCH4III ACNGT 2 cut(s) 11, 549
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 7 cut(s) 74, 199, 208, 604, 723, 855, 1068
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 484
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 6 cut(s) 347, 604, 670, 788, 812, 916
KflI GGGWCCC 1 cut(s) 778
Kpn2I TCCGGA 1 cut(s) 1085
KpnI GGTACC 1 cut(s) 425
Ksp22I TGATCA 1 cut(s) 1113
KspAI GTTAAC 1 cut(s) 501
Kzo9I GATC 5 cut(s) 46, 59, 90, 110, 1113
LguI GCTCTTC 1 cut(s) 674
MaeI CTAG 4 cut(s) 248, 495, 570, 1047
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 1 cut(s) 787
MalI GATC 5 cut(s) 48, 61, 92, 112, 1115
MboI GATC 5 cut(s) 46, 59, 90, 110, 1113
MboII GAAGA 7 cut(s) 424, 608, 691, 727, 743, 1115, 1137
MflI RGATCY 2 cut(s) 59, 90
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 2 cut(s) 914, 947
MmeI TCCRAC 3 cut(s) 37, 81, 170
MnlI CCTC 6 cut(s) 141, 328, 474, 727, 851, 1015
Mox20I TGGCCA 1 cut(s) 305
MroI TCCGGA 1 cut(s) 1085
MscI TGGCCA 1 cut(s) 305
MseI TTAA 6 cut(s) 500, 506, 990, 1014, 1031, 1109
MslI CAYNNNNRTG 3 cut(s) 472, 591, 860
Msp20I TGGCCA 1 cut(s) 305
MspCI CTTAAG 1 cut(s) 989
MspI CCGG 2 cut(s) 296, 1086
MspR9I CCNGG 2 cut(s) 217, 278
MvaI CCWGG 2 cut(s) 217, 278
MwoI GCNNNNNNNGC 2 cut(s) 205, 484
NdeII GATC 5 cut(s) 46, 59, 90, 110, 1113
NlaIII CATG 6 cut(s) 347, 604, 670, 788, 812, 916
NlaIV GGNNCC 5 cut(s) 423, 750, 766, 779, 780
NmeAIII GCCGAG 2 cut(s) 504, 710
NmuCI GTSAC 1 cut(s) 787
NspI RCATGY 3 cut(s) 604, 788, 812
OliI CACNNNNGTG 2 cut(s) 591, 860
PciI ACATGT 2 cut(s) 784, 808
PciSI GCTCTTC 1 cut(s) 674
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 881
PfeI GAWTC 4 cut(s) 104, 193, 583, 845
PflMI CCANNNNNTGG 3 cut(s) 56, 312, 774
PleI GAGTC 2 cut(s) 913, 946
PmaCI CACGTG 1 cut(s) 862
PmlI CACGTG 1 cut(s) 862
PpsI GAGTC 2 cut(s) 913, 946
Ppu21I YACGTR 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 778
PscI ACATGT 2 cut(s) 784, 808
PshAI GACNNNNGTC 1 cut(s) 328
PshBI ATTAAT 1 cut(s) 1109
PsiI TTATAA 1 cut(s) 516
Psp5II RGGWCCY 1 cut(s) 778
Psp6I CCWGG 2 cut(s) 215, 276
PspCI CACGTG 1 cut(s) 862
PspGI CCWGG 2 cut(s) 215, 276
PspN4I GGNNCC 5 cut(s) 423, 750, 766, 779, 780
PspPI GGNCC 4 cut(s) 128, 171, 765, 778
PspPPI RGGWCCY 1 cut(s) 778
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 2 cut(s) 59, 90
RsaI GTAC 3 cut(s) 284, 423, 880
RsaNI GTAC 3 cut(s) 283, 422, 879
RseI CAYNNNNRTG 3 cut(s) 472, 591, 860
SalI GTCGAC 2 cut(s) 5, 17
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 6 cut(s) 500, 506, 990, 1014, 1031, 1109
Sau3AI GATC 5 cut(s) 46, 59, 90, 110, 1113
Sau96I GGNCC 4 cut(s) 128, 171, 765, 778
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 2 cut(s) 914, 947
ScrFI CCNGG 2 cut(s) 217, 278
SfcI CTRYAG 2 cut(s) 94, 197
SinI GGWCC 4 cut(s) 128, 171, 765, 778
SmiMI CAYNNNNRTG 3 cut(s) 472, 591, 860
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
SsiI CCGC 3 cut(s) 65, 169, 591
SspI AATATT 2 cut(s) 138, 225
SspMI CTAG 4 cut(s) 248, 495, 570, 1047
StyD4I CCNGG 2 cut(s) 215, 276
TaaI ACNGT 2 cut(s) 11, 549
TaiI ACGT 1 cut(s) 864
TaqI TCGA 5 cut(s) 6, 18, 242, 402, 843
TatI WGTACW 2 cut(s) 282, 878
TfiI GAWTC 4 cut(s) 104, 193, 583, 845
Tru1I TTAA 6 cut(s) 500, 506, 990, 1014, 1031, 1109
Tru9I TTAA 6 cut(s) 500, 506, 990, 1014, 1031, 1109
TseFI GTSAC 1 cut(s) 787
Tsp45I GTSAC 1 cut(s) 787
TspDTI ATGAA 4 cut(s) 29, 382, 655, 856
Van91I CCANNNNNTGG 3 cut(s) 56, 312, 774
Vha464I CTTAAG 1 cut(s) 989
VpaK11BI GGWCC 4 cut(s) 128, 171, 765, 778
VspI ATTAAT 1 cut(s) 1109
XapI RAATTY 7 cut(s) 35, 76, 349, 359, 996, 1010, 1027
XbaI TCTAGA 1 cut(s) 1046
XceI RCATGY 3 cut(s) 604, 788, 812
XmiI GTMKAC 2 cut(s) 6, 18
XspI CTAG 4 cut(s) 248, 495, 570, 1047
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.