pycom08g18400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0020369
Physical Location & Seq
Forward (+)
18609204 .. 18610579
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18400.7

Sequence Viewer

Length: 870 bp
ATGGCATTAGTTGATGCGAAGATTGCACGAAGTGGAGATTTTTCCAAGCCTTCCGACATCCTGGTATCTAAAAGTTTTTTACCCAAAATGGTCCCTGATAAGTGGTCCTTGAGATTGTCCACCATCCATCATTTTGGTCATTCTGTTAAAAACTCCGTTAAGTGTCCAGCAATTTTCAAAGCTTCAGGAAAGTGTAAAATAAAATTATACCTATTTGAAAGCCCAATGGTTACTAGAGATTATCGGAAAATAGCCTCAAAGTTGGTTAGTCCGAGGGAAAATTGGAAAACTCGCCGGAAACTATCAAAAATGAAAATCATACTTCTAGAAGAGACGATGGTACCTTTTTCAACAGCTAACTCTCCATGGTTTTGCCGGAAAATGACTCGAAAATGGTTGTCTTGGTCTCAAGTTAGCCACTTTCGAGCCATTTTTCGGTCAAAACATGTTTCATTTTTGAATCACTTAATTTCTTTCCGGCGAGTTTTCCAGTTTTCCCTCGGACCGGTCAACATTGAGGCTATTTTCCAACCATATCCGGCAACCATTGGTTTGATATATTATGGGTCGAATTTGGCTAAGAAACGATTGAGTTACGAAACTTTGAAAATTGACCAAAACGATGGATGGTGGACAATCTCAGGGACCATTTCTATTGATTTGAAATCTCAGGGACCAAAAAAACTACTTTTCCACAGTGACTTAAAACAAATTGTAAATGTTTGTCTTCTTTGGAATGTAGATTATGTTATGAAATTTCAGGCATTTGATCTTGTTCAAGGAGCACACATAAGTGTTGTTCAGATTCTAACCCATTTATATAAAAATTGCGTACTTTTGTTCTGCCCCACTACCTATGTTAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

33.41

Weight (kDa)

10.04

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020369)

Species Orthologous Gene IDs
malus_domestica MD03G1116600.v1.1
pyrus_communis pycom07g12140 pycom08g18400 pycom13g27360 pycom13g27370 pycom17g18000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 340
AccB1I GGYRCC 1 cut(s) 340
AcsI RAATTY 3 cut(s) 571, 755, 864
AcuI CTGAAG 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 32
AfaI GTAC 2 cut(s) 342, 834
AfiI CCNNNNNNNGG 2 cut(s) 435, 505
AflIII ACRYGT 1 cut(s) 445
AgeI ACCGGT 1 cut(s) 505
AgsI TTSAA 7 cut(s) 178, 218, 351, 460, 607, 664, 779
AjnI CCWGG 1 cut(s) 60
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 2 cut(s) 182, 356
AluI AGCT 2 cut(s) 182, 356
Alw21I GWGCWC 1 cut(s) 787
Alw26I GTCTC 2 cut(s) 326, 411
ApoI RAATTY 3 cut(s) 571, 755, 864
AsiGI ACCGGT 1 cut(s) 505
Asp718I GGTACC 1 cut(s) 340
AspS9I GGNCC 5 cut(s) 91, 105, 503, 645, 674
AvaII GGWCC 5 cut(s) 91, 105, 503, 645, 674
BaeI ACNNNNGTAYC 2 cut(s) 324, 357
BanI GGYRCC 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 719
Bbv12I GWGCWC 1 cut(s) 787
BccI CCATC 5 cut(s) 131, 135, 331, 617, 621
BciT130I CCWGG 1 cut(s) 62
BcoDI GTCTC 2 cut(s) 326, 411
BfaI CTAG 2 cut(s) 234, 326
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 5 cut(s) 91, 105, 503, 645, 674
BmgT120I GGNCC 5 cut(s) 91, 105, 503, 645, 674
BmiI GGNNCC 4 cut(s) 93, 342, 646, 675
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 1 cut(s) 4
BpiI GAAGAC 1 cut(s) 719
BpuEI CTTGAG 2 cut(s) 130, 393
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 272, 365, 499
BsaWI WCCGGW 1 cut(s) 505
Bsc4I CCNNNNNNNGG 2 cut(s) 435, 505
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 1 cut(s) 490
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 3 cut(s) 272, 365, 499
BseGI GGATG 3 cut(s) 57, 123, 632
BseLI CCNNNNNNNGG 2 cut(s) 435, 505
BseMII CTCAG 2 cut(s) 654, 683
BseNI ACTGG 1 cut(s) 490
BshNI GGYRCC 1 cut(s) 340
BshTI ACCGGT 1 cut(s) 505
BsiHKAI GWGCWC 1 cut(s) 787
BsiSI CCGG 5 cut(s) 295, 376, 478, 506, 539
BslFI GGGAC 3 cut(s) 77, 658, 687
BslI CCNNNNNNNGG 2 cut(s) 435, 505
BsmAI GTCTC 2 cut(s) 326, 411
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 3 cut(s) 77, 658, 687
Bso31I GGTCTC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 787
Bsp143I GATC 1 cut(s) 769
Bsp19I CCATGG 1 cut(s) 365
BspCNI CTCAG 2 cut(s) 653, 682
BspLI GGNNCC 4 cut(s) 93, 342, 646, 675
BspT107I GGYRCC 1 cut(s) 340
BspTNI GGTCTC 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 505
BsrI ACTGG 1 cut(s) 490
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 3 cut(s) 272, 365, 499
BssMI GATC 1 cut(s) 769
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 1 cut(s) 324
BstDEI CTNAG 3 cut(s) 579, 640, 669
BstDSI CCRYGG 1 cut(s) 365
BstF5I GGATG 3 cut(s) 57, 123, 632
BstKTI GATC 1 cut(s) 772
BstMAI GTCTC 2 cut(s) 326, 411
BstMBI GATC 1 cut(s) 769
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 60
BstV2I GAAGAC 1 cut(s) 719
BstXI CCANNNNNNTGG 2 cut(s) 134, 623
BtgI CCRYGG 1 cut(s) 365
BtsCI GGATG 3 cut(s) 57, 123, 632
BtsIMutI CAGTG 1 cut(s) 703
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 5 cut(s) 91, 105, 503, 645, 674
CpoI CGGWCCG 1 cut(s) 503
Csp6I GTAC 2 cut(s) 341, 833
CspAI ACCGGT 1 cut(s) 505
CspI CGGWCCG 1 cut(s) 503
CviAII CATG 2 cut(s) 366, 446
CviJI RGCY 9 cut(s) 49, 182, 222, 254, 356, 417, 428, 521, 578
CviKI_1 RGCY 9 cut(s) 49, 182, 222, 254, 356, 417, 428, 521, 578
CviQI GTAC 2 cut(s) 341, 833
DdeI CTNAG 3 cut(s) 579, 640, 669
DpnI GATC 1 cut(s) 771
DpnII GATC 1 cut(s) 769
DraIII CACNNNGTG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 411
Eco47I GGWCC 5 cut(s) 91, 105, 503, 645, 674
Eco57I CTGAAG 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 365
ErhI CCWWGG 1 cut(s) 365
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 369, 449
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 3 cut(s) 77, 658, 687
FatI CATG 2 cut(s) 365, 445
FokI GGATG 3 cut(s) 44, 110, 639
FspBI CTAG 2 cut(s) 234, 326
HapII CCGG 5 cut(s) 295, 376, 478, 506, 539
Hin1II CATG 2 cut(s) 369, 449
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 3 cut(s) 385, 460, 805
HpaII CCGG 5 cut(s) 295, 376, 478, 506, 539
Hpy166II GTNNAC 3 cut(s) 120, 511, 633
Hpy188I TCNGA 5 cut(s) 55, 246, 273, 503, 804
Hpy188III TCNNGA 2 cut(s) 186, 326
Hpy8I GTNNAC 3 cut(s) 120, 511, 633
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 3 cut(s) 579, 640, 669
Hsp92II CATG 2 cut(s) 369, 449
KpnI GGTACC 1 cut(s) 344
Kzo9I GATC 1 cut(s) 769
LmnI GCTCC 1 cut(s) 782
LweI GCATC 1 cut(s) 4
MaeI CTAG 2 cut(s) 234, 326
MaeIII GTNAC 3 cut(s) 229, 593, 698
MalI GATC 1 cut(s) 771
MboI GATC 1 cut(s) 769
MboII GAAGA 3 cut(s) 31, 341, 719
MhlI GDGCHC 1 cut(s) 787
MlyI GAGTC 1 cut(s) 379
MmeI TCCRAC 2 cut(s) 78, 553
MnlI CCTC 4 cut(s) 265, 267, 509, 511
MseI TTAA 6 cut(s) 147, 159, 467, 704, 861, 868
MslI CAYNNNNRTG 1 cut(s) 792
MspI CCGG 5 cut(s) 295, 376, 478, 506, 539
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 23
NcoI CCATGG 1 cut(s) 365
NdeII GATC 1 cut(s) 769
NlaIII CATG 2 cut(s) 369, 449
NlaIV GGNNCC 4 cut(s) 93, 342, 646, 675
NmuCI GTSAC 1 cut(s) 698
NspI RCATGY 1 cut(s) 449
OliI CACNNNNGTG 1 cut(s) 792
PciI ACATGT 1 cut(s) 445
PfeI GAWTC 2 cut(s) 460, 805
PinAI ACCGGT 1 cut(s) 505
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
PscI ACATGT 1 cut(s) 445
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 4 cut(s) 93, 342, 646, 675
PspPI GGNCC 5 cut(s) 91, 105, 503, 645, 674
RsaI GTAC 2 cut(s) 342, 834
RsaNI GTAC 2 cut(s) 341, 833
RseI CAYNNNNRTG 1 cut(s) 792
Rsr2I CGGWCCG 1 cut(s) 503
RsrII CGGWCCG 1 cut(s) 503
SaqAI TTAA 6 cut(s) 147, 159, 467, 704, 861, 868
Sau3AI GATC 1 cut(s) 769
Sau96I GGNCC 5 cut(s) 91, 105, 503, 645, 674
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 1 cut(s) 787
SetI ASST 5 cut(s) 184, 213, 346, 358, 857
SfaNI GCATC 1 cut(s) 4
SinI GGWCC 5 cut(s) 91, 105, 503, 645, 674
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 2 cut(s) 109, 408
SmoI CTYRAG 2 cut(s) 109, 408
SspMI CTAG 2 cut(s) 234, 326
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 1 cut(s) 698
TaqI TCGA 3 cut(s) 388, 424, 569
TaqII GACCGA 1 cut(s) 426
TfiI GAWTC 2 cut(s) 460, 805
Tru1I TTAA 6 cut(s) 147, 159, 467, 704, 861, 868
Tru9I TTAA 6 cut(s) 147, 159, 467, 704, 861, 868
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 1 cut(s) 698
Tsp45I GTSAC 1 cut(s) 698
TspDTI ATGAA 3 cut(s) 326, 441, 767
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 1 cut(s) 703
VpaK11BI GGWCC 5 cut(s) 91, 105, 503, 645, 674
XapI RAATTY 3 cut(s) 571, 755, 864
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 1 cut(s) 449
XspI CTAG 2 cut(s) 234, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.