pycom07g17690

Leukocyte receptor cluster

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009843
Physical Location & Seq
Forward (+)
20220725 .. 20229280
8556 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3624 bp
ATGGCAACCCACGAGATCCTACTAAACCAGCAAACACGACCCAGGACCCAACCCAGACGCCCGGTCGTTGAACCTCAAACCCTCCCTCCCTTCCACCTCCTTTCTCTCTCATCTCTCTCCCTCTCTCTCTCTCTCTCTCTCTGCAATTTTCTTCCGCATATCACTATCGCTGTTCTCGCCAATCTGTCCCTAATAATTTTTATTAATTTTTCCTGCTTTTTATTCAATTTCATTCATGCTATTTGGTTTAAATATTTAGGTTTTTTCAGTACAAATTCTTGTTTTTCTGGTTCTTCTTGCTCGTTTTTGTTGTTGATCTCAATCTGTTTTCTGGGTTTTTCTCCACCCTCCGATCCCATCCATCAGCGTTCTCGTAAGTCAAACTGCATTTCGAGGTTGATACCCTGCGTTTTGTCTGAAGATATGATGAATCAAGGAGGTAATACTGAGAAGATTGCTTCTCTGGACCCGAATTCGCTTGAGAATCGGTATATTGTTAATGCAAACCAAGGACAGACGCCTTCATATACTCTTTCAACAACTGGTTCAGAAGCTTCATCCTGGAACCTTCATAGGACAGACAATAGCTCGACAGATAATGGGGTTCATTCCCATTCAAATTATCAATATGATCAGCATCCACAGCCGCCTGGGAGTAGTACATCAAATTTGGGAACAGTAAATGCACCACAAGATTACAATAGCTATGCTTCGTACCAAAATTCTGCGGATCCATATGGTTATGGAAGTGCAGGGTACCCAGGTTACTATGACAACTATCAGCAGCAATCCAACCCTTCCTACTCACAGCCCGTAGGAGCATATCAAAATACAGGTGCTCCTTATCAGCCTATTTCCTCATTTCAGAATACAGGGTCTAATGCTGGGTCTGCAAGTTATTCAAGCACTTACTACAATCCTGCTGATTATCAGACAGCTGGAGGTTACCCAAGTAGCAGCTACAATAATCAGACAACTGCTTGGAGTGGCGGAAATTATGCAAATTATACGCCTCATCAGTACTCACAGTACACCCCAGATACCACTGCTGCATATAGTTCTAGTGCTGCAACTTCAACTTCACAAAATTATGAGCAGCACTATAAGCAATGGGCTGATTATTACAGTCAAACAGAAGTCAGCTGTGCTCCTGGTACTGAGAACATTTCTGTTACTAGTACACCCAATGTGGGATGTCCGGTTCCAGGTGCTACCACTGGGTATCAAACCTCGGACAGCCAGCTACCACCACCACCACCACTACCTTCATACGCTCCTTCTTGGAAGCCAGAACCCAGTCCATCTGAATTGTCCTCAGTACAGTCTGGTGCTGTGGTTTTTGGTGCTCATGATGGTTACTGGAATCATGGGGCTCTGACTTCTCAATCTCAAGTTCACCACAATAGTTCTATGCAACCACACTTCCAAAAGCCTTTGGATGAGAAGACTTCTTATGATAGCTTTCAGGATCACCAGAAAACTGCATTTTCTCAAGCACTCAACATGCAGTATCCTGCTTCTCAGCAGGTGCCCCATGCATCTCATACTTACCAATCACCCTCGCAACCTGTTACATCCCATGCGTCTCATACTTACCAATCACCCTCACAACCTGTTCCATCCCATGCATCTCATACTTATCAATCGCCGTCCCAACCTGTTCCATATGGGTCTCATACTTATCAATCACCCTTACAACCTGTTCCATCCCATGCGTCTCATACTTACCAATCACCCTCCCAACCTGCTCCACCTGTAGGTACACAAAGAGTTAATAAACTGCAGATTCCAACAAACCCTAGAATCACTTCAAATCCAACCTTTGGTCTACCAAAAACTGACAAGGATAGCTGTACAACCACTGTGGCAGCAAAACCTGCTTATATCAGTGTTTCACTGCCAAAGCCAGTTGACAAGGTGACATCCAGTGCCACAGCTGATTCTCTACTTAAGCCTGGTATGTTTCCCAAGTCACTGCGTGGTTATGTCGAAAGGGCTTTGGCTCGCTGTAAAGATGATACGCAAATGGCTGCGTGTCAGTCTGTCATGAAGGAGATTATCACCAAGGCAACAGCTGATGGTACACTTTATACGCAAGACTGGGATACTGAGCCTCTTTTCCCACTGCCAAATGAAGATGCTGTTAAGAAAGATAGTTTACATTCTTCAAACCTTGTTTCATCATTGCCAAAGTACAAAAGAAGTCCGAATAGACGATCCAAGAGTAGATGGGAACCTCTACCAGAGGAGAAGCCAGTTGAGAAACCGGCATCTGTCAACAATGACAGTTTAAAGTTTTCTTGGATGAATGTCAATGATAAGCAAAGAAAGCCATGGATGGGGAGTGCTGGGTTCAAAGATGGCAATACAAGTAATGGAAAGTTTAATTCACAGGAGCAGAAAAATGGAAGTAAGATAAACCAGAAACCTTTTAAGAAGCAGCGCCTTTCTTATGTAAGTACTGCTGAGAACGGTGATGCATCTAGTGATAGTGACAAGGAACAAAGTTCATCAGCATATTACACTGGAGCAATGGACCTTGCGGATTCACCAGAGGAAAGAAAGAGACGTGAAAATCGCTCTAGGCGTTTTGAAAGAGTGCAAGGACATAGGGCACAAAATAATCACTTCAAACCAAAAAAAGCTGGTGGCGGAAACTTGTATGCCAGAAGGGCTAATGCCTTGGTGCTTAGCAAAAATTTTGAAGATGGTGGCAGCAGGGCAGTTGAGGACATTGACTGGGATTCTCTTACTGTTAAGGGGACCTGCCAGGAGATTGAGAAACGTTATTTGCGCCTCACTTCTGCTGCTGATCCTGCCACTGTAAGGCCAGAGGATGTTCTTGAAAAAGCTTTGCTTATGGTGCAAGATTCCCAAAAGAATTACCTATATAAATGTGATCAGTTGAAGTCCATTCGTCAGGATCTGACTGTGCAACGAATTCGCAATCACCTAACAGTAAAGGTGTACGAAACCCATGCTCGCTTAGCAGTGGAAGTTGGGGACCTGCCCGAGTATAATCAGTGCGCGTCACAGTTGAAATCTCTCTACGCTGAAGGTATTGAGGGATGTCATATGGAGTTCTCTGCATACAACTTACTTTGTGTTATCCTTCACTCAAATAATAACAGAGATCTAGTATCATCAATGGCAAGCTTGTCAGTTGAAGCAAAAAGAGATGAAGCTGTTAAGCATGCTCTTGCGGTTCGTTCAGCTGTTACTTCAGGAAATTATGTTATGTTCTTCAGGCTCTACAAGACAGCTCCCAACTTGAGCACCTGCCTAATGGATTTATATGTTGAAAAGATGCGGTATAAGGCAGTGAGCTGCATGTGTCGATCATATCGGCCTACGATACCTGTTCCTTATGTTGCACAAATTTTGGGATTTACCACCATTACACCCACAAATGAGGAAAGTGAGGAGAAGGATTCGGAGGGATTGGACGAATGCATTGAATGGTTGAAAGTACACGGTGCGTGCCTCGTTGTAGATAACAATGGAGAAATGCAGCTTGATACGAAGCCTACATCCTCGAGTCTTTACATGCCGGAGACTGATGCGGTGTCCCATGGAGACGCCACTCTTGCTGTTAACGATTTTTTGACGAGGACAACTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAC3_GANP

Protein Analysis

1208

Amino Acids

133.48

Weight (kDa)

6.94

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 930 - 1136 6.1e-23 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1516, 3287
Acc36I ACCTGC 6 cut(s) 1516, 1753, 1885, 2774, 3015, 3287
Acc65I GGTACC 1 cut(s) 756
AccB1I GGYRCC 2 cut(s) 756, 1528
AccB7I CCANNNNNTGG 1 cut(s) 1823
AccI GTMKAC 1 cut(s) 1828
AccII CGCG 1 cut(s) 3029
AciI CCGC 9 cut(s) 155, 647, 728, 990, 2543, 2652, 3203, 3310, 3563
AclI AACGTT 1 cut(s) 2785
AclWI GGATC 8 cut(s) 10, 347, 725, 738, 1476, 2211, 2807, 2931
AcsI RAATTY 7 cut(s) 274, 472, 667, 721, 2698, 2940, 3378
AcuI CTGAAG 4 cut(s) 438, 3075, 3207, 3229
AcyI GRCGYC 3 cut(s) 58, 518, 3579
AdeI CACNNNGTG 1 cut(s) 1979
AfiI CCNNNNNNNGG 6 cut(s) 1190, 1205, 1757, 1823, 2339, 2826
AflII CTTAAG 1 cut(s) 1949
AhdI GACNNNNNGTC 1 cut(s) 62
AhlI ACTAGT 1 cut(s) 1175
AjiI CACGTC 1 cut(s) 2570
AjnI CCWGG 8 cut(s) 41, 560, 649, 760, 1150, 1204, 1954, 2769
AloI GAACNNNNNNTCC 2 cut(s) 1185, 1217
Alw21I GWGCWC 4 cut(s) 841, 1150, 1348, 3278
Alw26I GTCTC 6 cut(s) 1590, 1677, 1722, 2560, 3548, 3570
AlwI GGATC 8 cut(s) 10, 347, 725, 738, 1476, 2211, 2807, 2931
AlwNI CAGNNNCTG 1 cut(s) 2926
Ama87I CYCGRG 2 cut(s) 3011, 3535
AoxI GGCC 2 cut(s) 2828, 3347
ApoI RAATTY 7 cut(s) 274, 472, 667, 721, 2698, 2940, 3378
AseI ATTAAT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 1807
Asp718I GGTACC 1 cut(s) 756
AspLEI GCGC 3 cut(s) 2445, 2796, 3029
AspS9I GGNCC 5 cut(s) 45, 466, 2536, 2763, 3004
AsuC2I CCSGG 1 cut(s) 62
AvaI CYCGRG 2 cut(s) 3011, 3535
AvaII GGWCC 5 cut(s) 45, 466, 2536, 2763, 3004
BaeGI GKGCMC 2 cut(s) 1533, 2617
BaeI ACNNNNGTAYC 2 cut(s) 748, 781
BamHI GGATCC 1 cut(s) 730
BanI GGYRCC 2 cut(s) 756, 1528
BanII GRGCYC 1 cut(s) 1375
BauI CACGAG 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 1451
Bbv12I GWGCWC 4 cut(s) 841, 1150, 1348, 3278
BceAI ACGGC 1 cut(s) 1633
BcgI CGANNNNNNTGC 4 cut(s) 2924, 2958, 2960, 2994
BciT130I CCWGG 8 cut(s) 43, 562, 651, 762, 1152, 1206, 1956, 2771
BciVI GTATCC 2 cut(s) 1521, 2098
BclI TGATCA 2 cut(s) 631, 2899
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 6 cut(s) 1590, 1677, 1722, 2560, 3548, 3570
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 6 cut(s) 1062, 1176, 1800, 2484, 2583, 3137
BfmI CTRYAG 2 cut(s) 1755, 1781
BfoI RGCGCY 1 cut(s) 2446
BfrI CTTAAG 1 cut(s) 1949
BfuAI ACCTGC 6 cut(s) 1516, 1753, 1885, 2774, 3015, 3287
BfuI GTATCC 2 cut(s) 1521, 2098
BglI GCCNNNNNGGC 1 cut(s) 2672
BglII AGATCT 1 cut(s) 3133
BlpI GCTNAGC 2 cut(s) 2690, 2986
BmcAI AGTACT 2 cut(s) 1022, 2461
Bme1390I CCNGG 9 cut(s) 43, 62, 562, 651, 762, 1152, 1206, 1956, 2771
Bme18I GGWCC 5 cut(s) 45, 466, 2536, 2763, 3004
BmeRI GACNNNNNGTC 1 cut(s) 62
BmeT110I CYCGRG 2 cut(s) 3011, 3535
BmgBI CACGTC 1 cut(s) 2570
BmgT120I GGNCC 5 cut(s) 45, 466, 2536, 2763, 3004
BmrFI CCNGG 9 cut(s) 43, 62, 562, 651, 762, 1152, 1206, 1956, 2771
BmrI ACTGGG 4 cut(s) 1227, 1290, 2110, 2749
BmsI GCATC 9 cut(s) 646, 1547, 1637, 2128, 2279, 2467, 2489, 3297, 3550
BmuI ACTGGG 4 cut(s) 1227, 1290, 2110, 2749
BpiI GAAGAC 1 cut(s) 1451
BpmI CTGGAG 2 cut(s) 960, 2547
Bpu1102I GCTNAGC 2 cut(s) 2690, 2986
BpuEI CTTGAG 4 cut(s) 500, 1374, 1476, 3292
BpuMI CCSGG 1 cut(s) 62
BsaBI GATNNNNATC 2 cut(s) 320, 636
BsaHI GRCGYC 3 cut(s) 58, 518, 3579
BsaI GGTCTC 1 cut(s) 1677
BsaJI CCNNGG 9 cut(s) 41, 508, 650, 760, 1230, 2064, 2333, 2682, 3571
BsaWI WCCGGW 1 cut(s) 1198
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 823, 853
Bsc4I CCNNNNNNNGG 6 cut(s) 1190, 1205, 1757, 1823, 2339, 2826
Bse118I RCCGGY 1 cut(s) 2266
Bse3DI GCAATG 3 cut(s) 1115, 2183, 2538
Bse8I GATNNNNATC 2 cut(s) 320, 636
BseBI CCWGG 8 cut(s) 43, 562, 651, 762, 1152, 1206, 1956, 2771
BseDI CCNNGG 9 cut(s) 41, 508, 650, 760, 1230, 2064, 2333, 2682, 3571
BseJI GATNNNNATC 2 cut(s) 320, 636
BseLI CCNNNNNNNGG 6 cut(s) 1190, 1205, 1757, 1823, 2339, 2826
BseMI GCAATG 3 cut(s) 1115, 2183, 2538
BseMII CTCAG 6 cut(s) 438, 1149, 1329, 1535, 2100, 2457
BseRI GAGGAG 2 cut(s) 2261, 3437
BseSI GKGCMC 2 cut(s) 1533, 2617
BseYI CCCAGC 2 cut(s) 884, 2348
BsgI GTGCAG 1 cut(s) 771
Bsh1236I CGCG 1 cut(s) 3029
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 2 cut(s) 2830, 3349
BshNI GGYRCC 2 cut(s) 756, 1528
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 4 cut(s) 841, 1150, 1348, 3278
BsiHKCI CYCGRG 2 cut(s) 3011, 3535
BsiSI CCGG 4 cut(s) 62, 1199, 2267, 3551
BslFI GGGAC 5 cut(s) 172, 1636, 2776, 3017, 3553
BslI CCNNNNNNNGG 6 cut(s) 1190, 1205, 1757, 1823, 2339, 2826
BsmAI GTCTC 6 cut(s) 1590, 1677, 1722, 2560, 3548, 3570
BsmBI CGTCTC 4 cut(s) 1590, 1722, 2560, 3570
BsmFI GGGAC 5 cut(s) 172, 1636, 2776, 3017, 3553
BsmI GAATGC 1 cut(s) 3455
BsnI GGCC 2 cut(s) 2830, 3349
Bso31I GGTCTC 1 cut(s) 1677
BsoBI CYCGRG 2 cut(s) 3011, 3535
Bsp1286I GDGCHC 7 cut(s) 841, 1150, 1348, 1375, 1533, 2617, 3278
Bsp1407I TGTACA 1 cut(s) 1853
Bsp1720I GCTNAGC 2 cut(s) 2690, 2986
Bsp19I CCATGG 2 cut(s) 2333, 3571
BspACI CCGC 9 cut(s) 155, 647, 728, 990, 2543, 2652, 3203, 3310, 3563
BspANI GGCC 2 cut(s) 2830, 3349
BspCNI CTCAG 6 cut(s) 439, 1150, 1328, 1534, 2101, 2458
BspFNI CGCG 1 cut(s) 3029
BspHI TCATGA 2 cut(s) 1348, 2046
BspMAI CTGCAG 1 cut(s) 1785
BspMI ACCTGC 6 cut(s) 1516, 1753, 1885, 2774, 3015, 3287
BspPI GGATC 8 cut(s) 10, 347, 725, 738, 1476, 2211, 2807, 2931
BspT107I GGYRCC 2 cut(s) 756, 1528
BspTI CTTAAG 1 cut(s) 1949
BspTNI GGTCTC 1 cut(s) 1677
BsrDI GCAATG 3 cut(s) 1115, 2183, 2538
BsrFI RCCGGY 1 cut(s) 2266
BsrGI TGTACA 1 cut(s) 1853
BssAI RCCGGY 1 cut(s) 2266
BssECI CCNNGG 9 cut(s) 41, 508, 650, 760, 1230, 2064, 2333, 2682, 3571
BssNI GRCGYC 3 cut(s) 58, 518, 3579
BssSI CACGAG 1 cut(s) 11
BssT1I CCWWGG 5 cut(s) 508, 2064, 2333, 2682, 3571
Bst2BI CACGAG 1 cut(s) 11
Bst2UI CCWGG 8 cut(s) 43, 562, 651, 762, 1152, 1206, 1956, 2771
BstACI GRCGYC 3 cut(s) 58, 518, 3579
BstAFI CTTAAG 1 cut(s) 1949
BstAPI GCANNNNNTGC 1 cut(s) 1877
BstAUI TGTACA 1 cut(s) 1853
BstC8I GCNNGC 6 cut(s) 1241, 2005, 2983, 3154, 3195, 3481
BstDEI CTNAG 8 cut(s) 447, 1158, 1315, 1521, 2109, 2466, 2690, 2986
BstDSI CCRYGG 2 cut(s) 2333, 3571
BstEII GGTNACC 1 cut(s) 944
BstFNI CGCG 1 cut(s) 3029
BstH2I RGCGCY 1 cut(s) 2446
BstHHI GCGC 3 cut(s) 2445, 2796, 3029
BstMAI GTCTC 6 cut(s) 1590, 1677, 1722, 2560, 3548, 3570
BstMCI CGRYCG 1 cut(s) 66
BstNI CCWGG 8 cut(s) 43, 562, 651, 762, 1152, 1206, 1956, 2771
BstNSI RCATGY 4 cut(s) 1507, 3197, 3334, 3550
BstPI GGTNACC 1 cut(s) 944
BstSCI CCNGG 9 cut(s) 41, 60, 560, 649, 760, 1150, 1204, 1954, 2769
BstSFI CTRYAG 2 cut(s) 1755, 1781
BstSLI GKGCMC 2 cut(s) 1533, 2617
BstUI CGCG 1 cut(s) 3029
BstV2I GAAGAC 1 cut(s) 1451
BstX2I RGATCY 4 cut(s) 15, 730, 2923, 3133
BstYI RGATCY 4 cut(s) 15, 730, 2923, 3133
BsuI GTATCC 2 cut(s) 1521, 2098
BsuRI GGCC 2 cut(s) 2830, 3349
BtgI CCRYGG 2 cut(s) 2333, 3571
BtrI CACGTC 1 cut(s) 2570
BtsI GCAGTG 6 cut(s) 1044, 1895, 1973, 2123, 2997, 3327
BveI ACCTGC 6 cut(s) 1516, 1753, 1885, 2774, 3015, 3287
Cac8I GCNNGC 6 cut(s) 1241, 2005, 2983, 3154, 3195, 3481
CaiI CAGNNNCTG 1 cut(s) 2926
CciI TCATGA 2 cut(s) 1348, 2046
CfoI GCGC 3 cut(s) 2445, 2796, 3029
Cfr10I RCCGGY 1 cut(s) 2266
Cfr13I GGNCC 5 cut(s) 45, 466, 2536, 2763, 3004
CseI GACGC 6 cut(s) 66, 526, 1572, 1704, 3018, 3587
CspCI CAANNNNNGTGG 2 cut(s) 1845, 1880
DdeI CTNAG 8 cut(s) 447, 1158, 1315, 1521, 2109, 2466, 2690, 2986
DraI TTTAAA 2 cut(s) 250, 2292
DraIII CACNNNGTG 1 cut(s) 1979
DriI GACNNNNNGTC 1 cut(s) 62
Eam1105I GACNNNNNGTC 1 cut(s) 62
EciI GGCGGA 2 cut(s) 1005, 2667
Eco130I CCWWGG 5 cut(s) 508, 2064, 2333, 2682, 3571
Eco24I GRGCYC 1 cut(s) 1375
Eco31I GGTCTC 1 cut(s) 1677
Eco47I GGWCC 5 cut(s) 45, 466, 2536, 2763, 3004
Eco57I CTGAAG 4 cut(s) 438, 3075, 3207, 3229
Eco88I CYCGRG 2 cut(s) 3011, 3535
Eco91I GGTNACC 1 cut(s) 944
EcoO109I RGGNCCY 3 cut(s) 45, 2763, 3004
EcoO65I GGTNACC 1 cut(s) 944
EcoRI GAATTC 2 cut(s) 472, 2940
EcoRII CCWGG 8 cut(s) 41, 560, 649, 760, 1150, 1204, 1954, 2769
EcoT14I CCWWGG 5 cut(s) 508, 2064, 2333, 2682, 3571
EcoT22I ATGCAT 4 cut(s) 1540, 1630, 2482, 3455
EcoT38I GRGCYC 1 cut(s) 1375
ErhI CCWWGG 5 cut(s) 508, 2064, 2333, 2682, 3571
Esp3I CGTCTC 4 cut(s) 1590, 1722, 2560, 3570
FalI AAGNNNNNCTT 2 cut(s) 2841, 2873
FaqI GGGAC 5 cut(s) 172, 1636, 2776, 3017, 3553
FauNDI CATATG 3 cut(s) 736, 1666, 3075
FbaI TGATCA 2 cut(s) 631, 2899
FblI GTMKAC 1 cut(s) 1828
FriOI GRGCYC 1 cut(s) 1375
FspBI CTAG 6 cut(s) 1062, 1176, 1800, 2484, 2583, 3137
GlaI GCGC 3 cut(s) 2444, 2795, 3028
GsaI CCCAGC 2 cut(s) 888, 2352
GsuI CTGGAG 2 cut(s) 960, 2547
HaeII RGCGCY 1 cut(s) 2446
HaeIII GGCC 2 cut(s) 2830, 3349
HapII CCGG 4 cut(s) 62, 1199, 2267, 3551
HgaI GACGC 6 cut(s) 66, 526, 1572, 1704, 3018, 3587
HhaI GCGC 3 cut(s) 2445, 2796, 3029
Hin1I GRCGYC 3 cut(s) 58, 518, 3579
Hin6I GCGC 3 cut(s) 2443, 2794, 3027
HinP1I GCGC 3 cut(s) 2443, 2794, 3027
HincII GTYRAC 3 cut(s) 1912, 2278, 3595
HindII GTYRAC 3 cut(s) 1912, 2278, 3595
HindIII AAGCTT 3 cut(s) 552, 2850, 3154
HpaI GTTAAC 1 cut(s) 3595
HpaII CCGG 4 cut(s) 62, 1199, 2267, 3551
Hpy188III TCNNGA 7 cut(s) 464, 1349, 1466, 2047, 2843, 2921, 3225
HpyCH4IV ACGT 2 cut(s) 2569, 2785
HpyF3I CTNAG 8 cut(s) 447, 1158, 1315, 1521, 2109, 2466, 2690, 2986
HpySE526I ACGT 2 cut(s) 2569, 2785
Hsp92I GRCGYC 3 cut(s) 58, 518, 3579
HspAI GCGC 3 cut(s) 2443, 2794, 3027
KpnI GGTACC 1 cut(s) 760
Ksp22I TGATCA 2 cut(s) 631, 2899
KspAI GTTAAC 1 cut(s) 3595
LmnI GCTCC 8 cut(s) 818, 844, 1153, 1279, 1753, 2395, 2528, 3268
LweI GCATC 9 cut(s) 646, 1547, 1637, 2128, 2279, 2467, 2489, 3297, 3550
MaeI CTAG 6 cut(s) 1062, 1176, 1800, 2484, 2583, 3137
MaeII ACGT 2 cut(s) 2569, 2785
MboII GAAGA 9 cut(s) 143, 285, 431, 463, 1456, 2147, 2157, 2717, 3235
MflI RGATCY 4 cut(s) 15, 730, 2923, 3133
MhlI GDGCHC 7 cut(s) 841, 1150, 1348, 1375, 1533, 2617, 3278
MlyI GAGTC 1 cut(s) 3547
MmeI TCCRAC 3 cut(s) 816, 1814, 1841
Mph1103I ATGCAT 4 cut(s) 1540, 1630, 2482, 3455
MroXI GAANNNNTTC 1 cut(s) 1807
MspA1I CMGCKG 5 cut(s) 938, 1143, 1937, 2075, 3215
MspCI CTTAAG 1 cut(s) 1949
MspI CCGG 4 cut(s) 62, 1199, 2267, 3551
MspR9I CCNGG 9 cut(s) 43, 62, 562, 651, 762, 1152, 1206, 1956, 2771
Mva1269I GAATGC 1 cut(s) 3455
MvaI CCWGG 8 cut(s) 43, 562, 651, 762, 1152, 1206, 1956, 2771
MvnI CGCG 1 cut(s) 3029
NciI CCSGG 1 cut(s) 62
NcoI CCATGG 2 cut(s) 2333, 3571
NdeI CATATG 3 cut(s) 736, 1666, 3075
NmuCI GTSAC 4 cut(s) 1918, 1971, 2492, 3030
NsiI ATGCAT 4 cut(s) 1540, 1630, 2482, 3455
NspI RCATGY 4 cut(s) 1507, 3197, 3334, 3550
PaeI GCATGC 1 cut(s) 3197
PaeR7I CTCGAG 1 cut(s) 3535
PagI TCATGA 2 cut(s) 1348, 2046
PaqCI CACCTGC 2 cut(s) 1516, 3287
PcsI WCGNNNNNNNCGW 1 cut(s) 2584
PctI GAATGC 1 cut(s) 3455
PdmI GAANNNNTTC 1 cut(s) 1807
PflMI CCANNNNNTGG 1 cut(s) 1823
PfoI TCCNGGA 1 cut(s) 560
PleI GAGTC 1 cut(s) 3546
PpsI GAGTC 1 cut(s) 3546
PpuMI RGGWCCY 3 cut(s) 45, 2763, 3004
PshBI ATTAAT 1 cut(s) 204
Psp1406I AACGTT 1 cut(s) 2785
Psp5II RGGWCCY 3 cut(s) 45, 2763, 3004
Psp6I CCWGG 8 cut(s) 41, 560, 649, 760, 1150, 1204, 1954, 2769
PspEI GGTNACC 1 cut(s) 944
PspFI CCCAGC 2 cut(s) 884, 2348
PspGI CCWGG 8 cut(s) 41, 560, 649, 760, 1150, 1204, 1954, 2769
PspPI GGNCC 5 cut(s) 45, 466, 2536, 2763, 3004
PspPPI RGGWCCY 3 cut(s) 45, 2763, 3004
PspXI VCTCGAGB 1 cut(s) 3535
PstI CTGCAG 1 cut(s) 1785
PstNI CAGNNNCTG 1 cut(s) 2926
PsuI RGATCY 4 cut(s) 15, 730, 2923, 3133
PvuII CAGCTG 5 cut(s) 938, 1143, 1937, 2075, 3215
Sau96I GGNCC 5 cut(s) 45, 466, 2536, 2763, 3004
ScaI AGTACT 2 cut(s) 1022, 2461
SchI GAGTC 1 cut(s) 3547
ScrFI CCNGG 9 cut(s) 43, 62, 562, 651, 762, 1152, 1206, 1956, 2771
SduI GDGCHC 7 cut(s) 841, 1150, 1348, 1375, 1533, 2617, 3278
SfaNI GCATC 9 cut(s) 646, 1547, 1637, 2128, 2279, 2467, 2489, 3297, 3550
SfcI CTRYAG 2 cut(s) 1755, 1781
Sfr274I CTCGAG 1 cut(s) 3535
SinI GGWCC 5 cut(s) 45, 466, 2536, 2763, 3004
SlaI CTCGAG 1 cut(s) 3535
SmlI CTYRAG 6 cut(s) 479, 1389, 1491, 1949, 3271, 3535
SmoI CTYRAG 6 cut(s) 479, 1389, 1491, 1949, 3271, 3535
SpeI ACTAGT 1 cut(s) 1175
SphI GCATGC 1 cut(s) 3197
SsiI CCGC 9 cut(s) 155, 647, 728, 990, 2543, 2652, 3203, 3310, 3563
SspI AATATT 1 cut(s) 254
SspMI CTAG 6 cut(s) 1062, 1176, 1800, 2484, 2583, 3137
StyD4I CCNGG 9 cut(s) 41, 60, 560, 649, 760, 1150, 1204, 1954, 2769
StyI CCWWGG 5 cut(s) 508, 2064, 2333, 2682, 3571
TaiI ACGT 2 cut(s) 2572, 2788
TaqI TCGA 5 cut(s) 392, 590, 1989, 3337, 3536
TauI GCSGC 1 cut(s) 649
TseFI GTSAC 4 cut(s) 1918, 1971, 2492, 3030
Tsp45I GTSAC 4 cut(s) 1918, 1971, 2492, 3030
Van91I CCANNNNNTGG 1 cut(s) 1823
Vha464I CTTAAG 1 cut(s) 1949
VpaK11BI GGWCC 5 cut(s) 45, 466, 2536, 2763, 3004
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 7 cut(s) 274, 472, 667, 721, 2698, 2940, 3378
XceI RCATGY 4 cut(s) 1507, 3197, 3334, 3550
XhoI CTCGAG 1 cut(s) 3535
XmiI GTMKAC 1 cut(s) 1828
XmnI GAANNNNTTC 1 cut(s) 1807
XspI CTAG 6 cut(s) 1062, 1176, 1800, 2484, 2583, 3137
ZrmI AGTACT 2 cut(s) 1022, 2461
Zsp2I ATGCAT 4 cut(s) 1540, 1630, 2482, 3455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.