pycom07g21020

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0021176
Physical Location & Seq
Forward (+)
22905446 .. 22907925
2480 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g21020.1

Sequence Viewer

Length: 546 bp
ATGCTTGTAGATGTTTGTGTGGGTTTCCTGGGTTACATTTTTGAAGAGTTGAAAGTCTCACCTTTTGAAGAGTTGAAATTCTCAACTTTTATATTTAGCCTTATATTTATGGAAGATTGTCAAGTAAGGTTATCATATTCCCCCTATTTTCAATCTTCTTCCTCCTCTCCTAAGACCTTGTCGAGGTCGATGGATGCAGAGGTATGGTCCTTACTGGAAGAAAGGACCCAGGAGATTTTGTGGACTATTCAACCAAATGTTGAATCTGAGTTGAGACGGAAGATGTTTGCTGATTATGTTCAGAGGCTTCTTGTAGGCCGTCTTGCAACTGAGCTTGCGTTACAGGTCTTCTCCTTTGGCTCAGTCCCATTGAAGGCCTATCTTCCTGATGGAGATATTGATTTGACTGCTCTCTGCCTTCCAAACTTGGTAGATATGCTTGCTCTAAAAATCTGTGCAATCCTCGAGGATCACCAACCACAGGACTCCAAGTTCCAAATTAAAGACGTCTGCTACGTTCGTGCACAGGACTTTTCTCCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

182

Amino Acids

20.74

Weight (kDa)

4.61

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021176)

Species Orthologous Gene IDs
malus_domestica MD01G1165100.v1.1
pyrus_communis pycom07g21020
rosa_chinensis RchiOBHm_Chr3g0485471
rosa_samantha Rh3AG267900 Rh3BG303400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 510
AbsI CCTCGAGG 1 cut(s) 464
AclWI GGATC 1 cut(s) 477
AcsI RAATTY 1 cut(s) 77
AcyI GRCGYC 1 cut(s) 507
AfiI CCNNNNNNNGG 3 cut(s) 183, 373, 481
AgsI TTSAA 8 cut(s) 44, 52, 68, 76, 152, 251, 263, 373
AjnI CCWGG 2 cut(s) 27, 228
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 1 cut(s) 334
AluI AGCT 1 cut(s) 334
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 2 cut(s) 61, 268
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 1 cut(s) 477
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 2 cut(s) 316, 375
ApaLI GTGCAC 1 cut(s) 522
ApoI RAATTY 1 cut(s) 77
AspS9I GGNCC 2 cut(s) 207, 225
AsuHPI GGTGA 2 cut(s) 51, 464
AvaI CYCGRG 1 cut(s) 464
AvaII GGWCC 2 cut(s) 207, 225
BaeGI GKGCMC 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 526
BccI CCATC 2 cut(s) 184, 383
BceAI ACGGC 1 cut(s) 303
BciT130I CCWGG 2 cut(s) 29, 230
BcoDI GTCTC 2 cut(s) 61, 268
Bme1390I CCNGG 2 cut(s) 29, 230
Bme18I GGWCC 2 cut(s) 207, 225
BmeT110I CYCGRG 1 cut(s) 464
BmgT120I GGNCC 2 cut(s) 207, 225
BmiI GGNNCC 1 cut(s) 227
BmrFI CCNGG 2 cut(s) 29, 230
BmsI GCATC 1 cut(s) 184
BpiI GAAGAC 1 cut(s) 340
BsaHI GRCGYC 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 28, 228
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 373, 481
Bse1I ACTGG 1 cut(s) 219
BseBI CCWGG 2 cut(s) 29, 230
BseDI CCNNGG 2 cut(s) 28, 228
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 3 cut(s) 183, 373, 481
BseMII CTCAG 3 cut(s) 258, 321, 375
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 526
BshFI GGCC 2 cut(s) 318, 377
BsiHKAI GWGCWC 1 cut(s) 526
BsiHKCI CYCGRG 1 cut(s) 464
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 183, 373, 481
BsmAI GTCTC 2 cut(s) 61, 268
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 2 cut(s) 318, 377
BsoBI CYCGRG 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 1 cut(s) 469
BspANI GGCC 2 cut(s) 318, 377
BspCNI CTCAG 3 cut(s) 259, 322, 374
BspLI GGNNCC 1 cut(s) 227
BspPI GGATC 1 cut(s) 477
BsrI ACTGG 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 28, 228
BssMI GATC 1 cut(s) 469
BssNI GRCGYC 1 cut(s) 507
Bst2UI CCWGG 2 cut(s) 29, 230
Bst6I CTCTTC 2 cut(s) 39, 63
BstACI GRCGYC 1 cut(s) 507
BstC8I GCNNGC 2 cut(s) 336, 441
BstDEI CTNAG 4 cut(s) 171, 267, 330, 361
BstENI CCTNNNNNAGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 199
BstKTI GATC 1 cut(s) 472
BstMAI GTCTC 2 cut(s) 61, 268
BstMBI GATC 1 cut(s) 469
BstNI CCWGG 2 cut(s) 29, 230
BstSCI CCNGG 2 cut(s) 27, 228
BstSLI GKGCMC 1 cut(s) 526
BstV2I GAAGAC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 318, 377
BtsCI GGATG 1 cut(s) 199
Cac8I GCNNGC 2 cut(s) 336, 441
Cfr13I GGNCC 2 cut(s) 207, 225
CviJI RGCY 6 cut(s) 99, 307, 318, 334, 360, 377
CviKI_1 RGCY 6 cut(s) 99, 307, 318, 334, 360, 377
DdeI CTNAG 4 cut(s) 171, 267, 330, 361
DpnI GATC 1 cut(s) 471
DpnII GATC 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 39, 63
EarI CTCTTC 2 cut(s) 39, 63
Eco147I AGGCCT 1 cut(s) 377
Eco47I GGWCC 2 cut(s) 207, 225
Eco88I CYCGRG 1 cut(s) 464
EcoNI CCTNNNNNAGG 1 cut(s) 181
EcoO109I RGGNCCY 1 cut(s) 225
EcoRII CCWGG 2 cut(s) 27, 228
Esp3I CGTCTC 1 cut(s) 268
FaiI YATR 7 cut(s) 92, 104, 110, 136, 205, 297, 437
FaqI GGGAC 1 cut(s) 350
FokI GGATG 1 cut(s) 206
HaeIII GGCC 2 cut(s) 318, 377
Hin1I GRCGYC 1 cut(s) 507
HinfI GANTC 2 cut(s) 263, 485
HphI GGTGA 2 cut(s) 51, 464
Hpy166II GTNNAC 2 cut(s) 243, 524
Hpy188I TCNGA 2 cut(s) 268, 303
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 2 cut(s) 243, 524
HpyAV CCTTC 2 cut(s) 367, 428
HpyCH4IV ACGT 2 cut(s) 507, 516
HpyCH4V TGCA 4 cut(s) 197, 326, 458, 524
HpyF3I CTNAG 4 cut(s) 171, 267, 330, 361
HpySE526I ACGT 2 cut(s) 507, 516
Hsp92I GRCGYC 1 cut(s) 507
Kzo9I GATC 1 cut(s) 469
LpnPI CCDG 9 cut(s) 14, 41, 200, 215, 242, 329, 399, 467, 512
LweI GCATC 1 cut(s) 184
MaeII ACGT 2 cut(s) 507, 516
MaeIII GTNAC 2 cut(s) 32, 339
MalI GATC 1 cut(s) 471
MboI GATC 1 cut(s) 469
MboII GAAGA 9 cut(s) 56, 80, 125, 147, 150, 230, 292, 340, 374
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 2 cut(s) 77, 498
MlyI GAGTC 1 cut(s) 479
MnlI CCTC 7 cut(s) 172, 175, 177, 193, 297, 460, 473
MseI TTAA 1 cut(s) 501
MspR9I CCNGG 2 cut(s) 29, 230
MvaI CCWGG 2 cut(s) 29, 230
NdeII GATC 1 cut(s) 469
NlaIV GGNNCC 1 cut(s) 227
PaeR7I CTCGAG 1 cut(s) 464
PceI AGGCCT 1 cut(s) 377
PcsI WCGNNNNNNNCGW 1 cut(s) 513
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 178
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 27, 228
PspGI CCWGG 2 cut(s) 27, 228
PspN4I GGNNCC 1 cut(s) 227
PspPI GGNCC 2 cut(s) 207, 225
PspPPI RGGWCCY 1 cut(s) 225
PspXI VCTCGAGB 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 178
SaqAI TTAA 1 cut(s) 501
Sau3AI GATC 1 cut(s) 469
Sau96I GGNCC 2 cut(s) 207, 225
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 29, 230
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 1 cut(s) 184
Sfr274I CTCGAG 1 cut(s) 464
SinI GGWCC 2 cut(s) 207, 225
SlaI CTCGAG 1 cut(s) 464
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 2 cut(s) 77, 498
SseBI AGGCCT 1 cut(s) 377
StuI AGGCCT 1 cut(s) 377
StyD4I CCNGG 2 cut(s) 27, 228
TaiI ACGT 2 cut(s) 510, 519
TaqI TCGA 3 cut(s) 182, 188, 465
TasI AATT 2 cut(s) 77, 498
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TspGWI ACGGA 1 cut(s) 292
Tth111I GACNNNGTC 1 cut(s) 178
VneI GTGCAC 1 cut(s) 522
VpaK11BI GGWCC 2 cut(s) 207, 225
XagI CCTNNNNNAGG 1 cut(s) 181
XapI RAATTY 1 cut(s) 77
XhoI CTCGAG 1 cut(s) 464
ZraI GACGTC 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.