pycom08g04260
RLK Family

Cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0022544
Physical Location & Seq
Forward (+)
3297473 .. 3298235
763 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04260.1

Sequence Viewer

Length: 639 bp
ATGCAATGTTCGAGATCATCTTGCTGCAGCTGGAAGGAAGGGAGAATGGGATATCTCTCAAAGACTTCAGGTCTCAACTCCTGGCTGAAGAAGCTATCATTGATCAGTCTTTTGACAGTTCTTCCGGATTGTCCTTTGGTAATGCCATGGTTACTGGAATTCAATCTGACAAAGGGAAAACACTTGTTCTTGATCAAGATCAACCACGGTCCTTCTCTGATCCATCTTTCAGGCACAATGGAGGATCCAGTTTTGCTAATTCCAATCATAATACTGGATCTTACAACATTGGGAGTTACCACAACTTTGGCAACTATAAGGGTAACAATTTCAGGGGCAAAGGGAGAGGCAGATTCCAATACAATTCAGGTCCCAGATTTTCCAACGGTAATCCATACATTCTTGGTACTCCAAAGCCATATCAATCTCACTGCCCGGATCGACCCTTCTGAGATTCCTACTTGTCAAATATGCAACAAAAAAGGTCATGTTGTTGCAGATTGCTTTCAAAGACATACCAACACTGCCTCGAGCTCTCCTATTCAATGTCAAATCTGTTGGAAATATGGACACTCAGCTCTTCAATGCTATCACAGAAATAATTTTACATGCCAATCATCAATCTTCCACATCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 gag_pre-integrs rve

Protein Analysis

213

Amino Acids

23.54

Weight (kDa)

8.66

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022544)

Species Orthologous Gene IDs
pyrus_communis pycom07g00390 pycom07g24750 pycom08g04260 pycom12g17440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 124
AclWI GGATC 5 cut(s) 214, 239, 252, 285, 446
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 2 cut(s) 51, 107
AfaI GTAC 1 cut(s) 408
AgsI TTSAA 4 cut(s) 163, 509, 545, 584
AhdI GACNNNNNGTC 1 cut(s) 69
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 4 cut(s) 30, 94, 534, 578
AluI AGCT 4 cut(s) 30, 94, 534, 578
Alw21I GWGCWC 1 cut(s) 536
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 5 cut(s) 214, 239, 252, 285, 446
Ama87I CYCGRG 1 cut(s) 529
Aor13HI TCCGGA 1 cut(s) 124
ApeKI GCWGC 2 cut(s) 24, 27
ApoI RAATTY 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 209, 370
AsuC2I CCSGG 1 cut(s) 436
AvaI CYCGRG 1 cut(s) 529
AvaII GGWCC 2 cut(s) 209, 370
BamHI GGATCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 536
BbvI GCAGC 2 cut(s) 11, 39
BccI CCATC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 2 cut(s) 102, 192
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 77
BfmI CTRYAG 1 cut(s) 25
BisI GCNGC 2 cut(s) 25, 28
BlsI GCNGC 2 cut(s) 26, 29
Bme1390I CCNGG 2 cut(s) 82, 436
Bme18I GGWCC 2 cut(s) 209, 370
BmeRI GACNNNNNGTC 1 cut(s) 69
BmeT110I CYCGRG 1 cut(s) 529
BmgT120I GGNCC 2 cut(s) 209, 370
BmiI GGNNCC 2 cut(s) 246, 372
BmrFI CCNGG 2 cut(s) 82, 436
BpuMI CCSGG 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 197
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 146, 205
BsaWI WCCGGW 1 cut(s) 124
Bse1I ACTGG 3 cut(s) 159, 248, 279
Bse3DI GCAATG 1 cut(s) 11
Bse8I GATNNNNATC 1 cut(s) 197
BseAI TCCGGA 1 cut(s) 124
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 2 cut(s) 146, 205
BseJI GATNNNNATC 1 cut(s) 197
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 2 cut(s) 441, 588
BseNI ACTGG 3 cut(s) 159, 248, 279
BseXI GCAGC 2 cut(s) 11, 39
BsiHKAI GWGCWC 1 cut(s) 536
BsiHKCI CYCGRG 1 cut(s) 529
BsiSI CCGG 2 cut(s) 125, 436
BslFI GGGAC 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 356
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 529
Bsp1286I GDGCHC 1 cut(s) 536
Bsp13I TCCGGA 1 cut(s) 124
Bsp143I GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
Bsp19I CCATGG 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 442, 587
BspEI TCCGGA 1 cut(s) 124
BspLI GGNNCC 2 cut(s) 246, 372
BspMAI CTGCAG 1 cut(s) 29
BspPI GGATC 5 cut(s) 214, 239, 252, 285, 446
BspQI GCTCTTC 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 11
BsrI ACTGG 3 cut(s) 159, 248, 279
BssECI CCNNGG 2 cut(s) 146, 205
BssMI GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 3 cut(s) 118, 209, 388
Bst6I CTCTTC 1 cut(s) 585
BstDEI CTNAG 2 cut(s) 450, 574
BstDSI CCRYGG 2 cut(s) 146, 205
BstKTI GATC 8 cut(s) 17, 105, 195, 201, 222, 247, 280, 441
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 2 cut(s) 80, 434
BstSFI CTRYAG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 11, 39
BstX2I RGATCY 2 cut(s) 244, 277
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 2 cut(s) 244, 277
BtgI CCRYGG 2 cut(s) 146, 205
BtsI GCAGTG 2 cut(s) 429, 522
BtsIMutI CAGTG 2 cut(s) 429, 522
Cfr13I GGNCC 2 cut(s) 209, 370
Csp6I GTAC 1 cut(s) 407
CviAII CATG 3 cut(s) 147, 488, 609
CviJI RGCY 6 cut(s) 30, 85, 94, 417, 534, 578
CviKI_1 RGCY 6 cut(s) 30, 85, 94, 417, 534, 578
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 2 cut(s) 450, 574
DpnI GATC 8 cut(s) 16, 104, 194, 200, 221, 246, 279, 440
DpnII GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
DriI GACNNNNNGTC 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 585
Eam1105I GACNNNNNGTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 585
Ecl136II GAGCTC 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 536
Eco31I GGTCTC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 53
Eco47I GGWCC 2 cut(s) 209, 370
Eco53kI GAGCTC 1 cut(s) 534
Eco57I CTGAAG 2 cut(s) 51, 107
Eco88I CYCGRG 1 cut(s) 529
EcoICRI GAGCTC 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 80
EcoRV GATATC 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 3 cut(s) 150, 491, 612
FaqI GGGAC 1 cut(s) 356
FatI CATG 3 cut(s) 146, 487, 608
FbaI TGATCA 2 cut(s) 102, 192
Fnu4HI GCNGC 2 cut(s) 25, 28
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 2 cut(s) 25, 28
GluI GCNGC 2 cut(s) 25, 28
HapII CCGG 2 cut(s) 125, 436
Hin1II CATG 3 cut(s) 150, 491, 612
HinfI GANTC 2 cut(s) 353, 454
HpaII CCGG 2 cut(s) 125, 436
Hpy188I TCNGA 4 cut(s) 168, 219, 451, 638
Hpy188III TCNNGA 4 cut(s) 12, 125, 190, 196
HpyAV CCTTC 4 cut(s) 28, 32, 222, 456
HpyCH4III ACNGT 3 cut(s) 118, 209, 388
HpyCH4V TGCA 4 cut(s) 4, 27, 474, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 2 cut(s) 450, 574
Hsp92II CATG 3 cut(s) 150, 491, 612
Kpn2I TCCGGA 1 cut(s) 124
Ksp22I TGATCA 2 cut(s) 102, 192
Kzo9I GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
LguI GCTCTTC 1 cut(s) 585
Lsp1109I GCAGC 2 cut(s) 11, 39
MaeIII GTNAC 3 cut(s) 150, 295, 322
MalI GATC 8 cut(s) 16, 104, 194, 200, 221, 246, 279, 440
MboI GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
MboII GAAGA 4 cut(s) 100, 113, 572, 616
MflI RGATCY 2 cut(s) 244, 277
MhlI GDGCHC 1 cut(s) 536
MluCI AATT 5 cut(s) 158, 258, 327, 363, 601
MmeI TCCRAC 2 cut(s) 407, 539
MnlI CCTC 3 cut(s) 235, 340, 538
MroI TCCGGA 1 cut(s) 124
MspA1I CMGCKG 1 cut(s) 30
MspI CCGG 2 cut(s) 125, 436
MspR9I CCNGG 2 cut(s) 82, 436
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 1 cut(s) 91
NciI CCSGG 1 cut(s) 436
NcoI CCATGG 1 cut(s) 146
NdeII GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
NlaIII CATG 3 cut(s) 150, 491, 612
NlaIV GGNNCC 2 cut(s) 246, 372
NspI RCATGY 1 cut(s) 612
PaeR7I CTCGAG 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 585
PfeI GAWTC 2 cut(s) 353, 454
PkrI GCNGC 2 cut(s) 26, 29
PpuMI RGGWCCY 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 246, 372
PspPI GGNCC 2 cut(s) 209, 370
PspPPI RGGWCCY 1 cut(s) 370
PspXI VCTCGAGB 1 cut(s) 529
PstI CTGCAG 1 cut(s) 29
PsuI RGATCY 2 cut(s) 244, 277
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SacI GAGCTC 1 cut(s) 536
SapI GCTCTTC 1 cut(s) 585
SatI GCNGC 2 cut(s) 25, 28
Sau3AI GATC 8 cut(s) 14, 102, 192, 198, 219, 244, 277, 438
Sau96I GGNCC 2 cut(s) 209, 370
ScrFI CCNGG 2 cut(s) 82, 436
SduI GDGCHC 1 cut(s) 536
SetI ASST 7 cut(s) 32, 73, 96, 372, 487, 536, 580
SfcI CTRYAG 1 cut(s) 25
Sfr274I CTCGAG 1 cut(s) 529
SinI GGWCC 2 cut(s) 209, 370
SlaI CTCGAG 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 5 cut(s) 158, 258, 327, 363, 601
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 80, 434
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 3 cut(s) 118, 209, 388
TaqI TCGA 3 cut(s) 11, 441, 530
TasI AATT 5 cut(s) 158, 258, 327, 363, 601
TfiI GAWTC 2 cut(s) 353, 454
TscAI CASTG 2 cut(s) 436, 529
TseI GCWGC 2 cut(s) 24, 27
TspRI CASTG 2 cut(s) 436, 529
VpaK11BI GGWCC 2 cut(s) 209, 370
XapI RAATTY 1 cut(s) 158
XceI RCATGY 1 cut(s) 612
XhoI CTCGAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.