pycom08g04530

Folate-Biopterin Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0021882
Physical Location & Seq
Reverse (-)
3438896 .. 3439435
540 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04530.1

Sequence Viewer

Length: 540 bp
ATGGATGATTACTCCTCTCAAGAAACCCAGAAAACAACCACCTCAAACCGATACGTCGATGATCAAGACGGTCACTACCATCCAAATCCCACCAACCCATTTGTCCAAATCCTCCAACAACCAATCCAATGGCTCCAAATGCTCTCCTCAGAGCTAAACCCTACCTTTGTCCTGGGTGTGGTTCTTGTTTACGGAGTCAGCCAGGGCTTCTCAGGCTCTTATTTCAAAGTGGTCACGGACTACTACTGGAAAGACGTCCAGAAACTCCAGCCCTCTGTTGTACAGCTTTACATTGGACTGTACTATATTCCATGGCTCATGAAGCCGGTTTGGGGCTTGTTGACCGACGTTTTTCCAGTCCGGGGATACAGAAGGCGACCTTATTTTGTCCTGGCCGGAGTTATAGGGACAGTTTCAGCTCTCACGGTTGCATTCAGTGGGAACTTGGCTGCTGTGGTGGCATTGACTTGGTTGATCGGCGTCACGGCAGGTGTGGCGATTGCGGATGTCATCGATCGACGCGTGTATAGCCCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

180

Amino Acids

20.16

Weight (kDa)

6.07

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BT1 PF03092 68 - 177 4e-24 BT1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10820
malus_domestica MD08G1055000.v1.1
prunus_persica Prupe.1G395300_v2.0.a1
pyrus_communis pycom08g04530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 479
AccII CGCG 1 cut(s) 522
AciI CCGC 1 cut(s) 503
AcoI YGGCCR 1 cut(s) 393
AcyI GRCGYC 2 cut(s) 255, 480
AfaI GTAC 2 cut(s) 282, 302
AfiI CCNNNNNNNGG 3 cut(s) 178, 332, 362
AflIII ACRYGT 1 cut(s) 520
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 3 cut(s) 171, 201, 390
AluBI AGCT 3 cut(s) 154, 286, 419
AluI AGCT 3 cut(s) 154, 286, 419
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 362, 533
BbvI GCAGC 1 cut(s) 436
BccI CCATC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 501
BciT130I CCWGG 3 cut(s) 173, 203, 392
BciVI GTATCC 1 cut(s) 359
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 362, 533
BfuAI ACCTGC 1 cut(s) 479
BfuI GTATCC 1 cut(s) 359
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 5 cut(s) 173, 203, 362, 392, 533
BmiI GGNNCC 1 cut(s) 134
BmrFI CCNGG 5 cut(s) 173, 203, 362, 392, 533
BpmI CTGGAG 1 cut(s) 251
BpuMI CCSGG 2 cut(s) 362, 533
Bsa29I ATCGAT 1 cut(s) 513
BsaHI GRCGYC 2 cut(s) 255, 480
BsaJI CCNNGG 4 cut(s) 172, 202, 311, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 332, 362
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 251, 356
BseBI CCWGG 3 cut(s) 173, 203, 392
BseCI ATCGAT 1 cut(s) 513
BseDI CCNNGG 4 cut(s) 172, 202, 311, 361
BseGI GGATG 3 cut(s) 10, 79, 511
BseLI CCNNNNNNNGG 3 cut(s) 178, 332, 362
BseMII CTCAG 2 cut(s) 162, 225
BseNI ACTGG 2 cut(s) 251, 356
BseRI GAGGAG 2 cut(s) 4, 136
BseXI GCAGC 1 cut(s) 436
Bsh1236I CGCG 1 cut(s) 522
Bsh1285I CGRYCG 1 cut(s) 517
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 513
BsiEI CGRYCG 1 cut(s) 517
BsiSI CCGG 4 cut(s) 326, 361, 396, 533
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 3 cut(s) 178, 332, 362
BsmFI GGGAC 1 cut(s) 421
BsmI GAATGC 1 cut(s) 431
BsnI GGCC 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 280
Bsp143I GATC 3 cut(s) 61, 474, 514
Bsp19I CCATGG 1 cut(s) 311
BspACI CCGC 1 cut(s) 503
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 161, 224
BspDI ATCGAT 1 cut(s) 513
BspFNI CGCG 1 cut(s) 522
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 479
BsrFI RCCGGY 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 2 cut(s) 251, 356
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 4 cut(s) 172, 202, 311, 361
BssMI GATC 3 cut(s) 61, 474, 514
BssNI GRCGYC 2 cut(s) 255, 480
BssT1I CCWWGG 1 cut(s) 311
Bst2UI CCWGG 3 cut(s) 173, 203, 392
Bst4CI ACNGT 4 cut(s) 71, 300, 412, 427
BstACI GRCGYC 2 cut(s) 255, 480
BstAUI TGTACA 1 cut(s) 280
BstDEI CTNAG 2 cut(s) 148, 211
BstDSI CCRYGG 1 cut(s) 311
BstF5I GGATG 3 cut(s) 10, 79, 511
BstFNI CGCG 1 cut(s) 522
BstKTI GATC 3 cut(s) 64, 477, 517
BstMBI GATC 3 cut(s) 61, 474, 514
BstMCI CGRYCG 1 cut(s) 517
BstMWI GCNNNNNNNGC 6 cut(s) 139, 213, 322, 458, 494, 528
BstNI CCWGG 3 cut(s) 173, 203, 392
BstSCI CCNGG 5 cut(s) 171, 201, 360, 390, 531
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 129
Bsu15I ATCGAT 1 cut(s) 513
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 311
BtsCI GGATG 3 cut(s) 10, 79, 511
BtsIMutI CAGTG 1 cut(s) 442
BveI ACCTGC 1 cut(s) 479
CciI TCATGA 1 cut(s) 318
Cfr10I RCCGGY 1 cut(s) 325
ClaI ATCGAT 1 cut(s) 513
CseI GACGC 2 cut(s) 469, 528
Csp6I GTAC 2 cut(s) 281, 301
CviAII CATG 2 cut(s) 312, 319
CviQI GTAC 2 cut(s) 281, 301
DdeI CTNAG 2 cut(s) 148, 211
DpnI GATC 3 cut(s) 63, 476, 516
DpnII GATC 3 cut(s) 61, 474, 514
EaeI YGGCCR 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 311
EcoRII CCWGG 3 cut(s) 171, 201, 390
EcoT14I CCWWGG 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 2 cut(s) 315, 322
FaiI YATR 5 cut(s) 306, 313, 320, 404, 528
FalI AAGNNNNNCTT 2 cut(s) 364, 396
FaqI GGGAC 1 cut(s) 421
FatI CATG 2 cut(s) 311, 318
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 3 cut(s) 17, 66, 518
Fsp4HI GCNGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 1 cut(s) 395
HapII CCGG 4 cut(s) 326, 361, 396, 533
HgaI GACGC 2 cut(s) 469, 528
Hin1I GRCGYC 2 cut(s) 255, 480
Hin1II CATG 2 cut(s) 315, 322
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HinfI GANTC 1 cut(s) 195
HpaII CCGG 4 cut(s) 326, 361, 396, 533
Hpy166II GTNNAC 2 cut(s) 190, 342
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 4 cut(s) 20, 65, 259, 319
Hpy8I GTNNAC 2 cut(s) 190, 342
Hpy99I CGWCG 3 cut(s) 59, 350, 522
HpyAV CCTTC 1 cut(s) 366
HpyCH4III ACNGT 4 cut(s) 71, 300, 412, 427
HpyCH4IV ACGT 3 cut(s) 54, 255, 348
HpyCH4V TGCA 1 cut(s) 431
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 213, 322, 458, 494, 528
HpyF3I CTNAG 2 cut(s) 148, 211
HpySE526I ACGT 3 cut(s) 54, 255, 348
Hsp92I GRCGYC 2 cut(s) 255, 480
Hsp92II CATG 2 cut(s) 315, 322
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 3 cut(s) 61, 474, 514
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 1 cut(s) 436
MaeII ACGT 3 cut(s) 54, 255, 348
MaeIII GTNAC 3 cut(s) 71, 232, 481
MalI GATC 3 cut(s) 63, 476, 516
MboI GATC 3 cut(s) 61, 474, 514
MluI ACGCGT 1 cut(s) 520
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 5 cut(s) 25, 52, 122, 157, 283
MspI CCGG 4 cut(s) 326, 361, 396, 533
MspR9I CCNGG 5 cut(s) 173, 203, 362, 392, 533
Mva1269I GAATGC 1 cut(s) 431
MvaI CCWGG 3 cut(s) 173, 203, 392
MvnI CGCG 1 cut(s) 522
MwoI GCNNNNNNNGC 6 cut(s) 139, 213, 322, 458, 494, 528
NciI CCSGG 2 cut(s) 362, 533
NcoI CCATGG 1 cut(s) 311
NdeII GATC 3 cut(s) 61, 474, 514
NlaIII CATG 2 cut(s) 315, 322
NlaIV GGNNCC 1 cut(s) 134
NmuCI GTSAC 3 cut(s) 71, 232, 481
PagI TCATGA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 519
PctI GAATGC 1 cut(s) 431
PkrI GCNGC 1 cut(s) 451
Ple19I CGATCG 1 cut(s) 517
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
Psp6I CCWGG 3 cut(s) 171, 201, 390
PspGI CCWGG 3 cut(s) 171, 201, 390
PspN4I GGNNCC 1 cut(s) 134
PvuI CGATCG 1 cut(s) 517
RsaI GTAC 2 cut(s) 282, 302
RsaNI GTAC 2 cut(s) 281, 301
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 3 cut(s) 61, 474, 514
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 5 cut(s) 173, 203, 362, 392, 533
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
SsiI CCGC 1 cut(s) 503
StyD4I CCNGG 5 cut(s) 171, 201, 360, 390, 531
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 4 cut(s) 71, 300, 412, 427
TaiI ACGT 3 cut(s) 57, 258, 351
TaqI TCGA 3 cut(s) 57, 513, 517
TaqII GACCGA 1 cut(s) 359
TatI WGTACW 2 cut(s) 280, 300
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 3 cut(s) 71, 232, 481
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 3 cut(s) 71, 232, 481
TspDTI ATGAA 1 cut(s) 335
TspGWI ACGGA 2 cut(s) 207, 251
TspRI CASTG 1 cut(s) 442
ZraI GACGTC 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.