pycom08g06010

Belongs to the glycosyl hydrolase 9 (cellulase E) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008332
Physical Location & Seq
Reverse (-)
4609756 .. 4610416
661 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
ATGATAGTAAGACTGTTCTTTGATGGTGAAAAATCCCTAGAAAACTACAAAAACGGAGCCGACAGTTACATTTGCTCAAACATCCCCGACAGCCCTTACCACCAAATCACAATGACACCGGGCGGTATGATCGATCACTTGAGAGACGGAGCCAACACCCAATACGTCACCGGAACAGCCCACTTATTCGCCGTCTACAGCGATATCCTCGCCCAACACAACCAAAAAGTCAACTGCGGAGGCAAAGAGTTCGACTCCGCCGCCCTCATGGCCTTTGCCAAGAAGCAAATGGACTACTTGTTGGGTGATAATCCCAAAAAAAGATCCTACATGGTCGGGTTTGGACCCAACGCGCCCCAACAACCCCACCACAGAGGAGCTTCGGTTCCGAAGGCGGAAAACGGCCTCGTTAACTGCGCCATGAGCTTCGTGTACTGGTTCAAGAAGGATGTGCCAAACCCTAATGAGTTGACTGGTGCCATCGTCGGTGGGCCCGATAGGTTTGATAACTTCGAGGACAAACGTTGGGCGTCGTCGTATACCGAGCCATGCACTTACGTGAACTCGCTCGCGGTCGGACCACTGGCGAAGCTTGCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

201

Amino Acids

22.03

Weight (kDa)

6.58

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 10 - 193 3.7e-45 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AccI GTMKAC 2 cut(s) 195, 539
AccII CGCG 2 cut(s) 353, 572
AciI CCGC 7 cut(s) 123, 237, 258, 261, 395, 572, 596
AclI AACGTT 1 cut(s) 523
AclWI GGATC 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 1 cut(s) 434
AgsI TTSAA 1 cut(s) 442
AleI CACNNNNGTG 1 cut(s) 557
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
AluBI AGCT 3 cut(s) 380, 426, 592
AluI AGCT 3 cut(s) 380, 426, 592
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 1 cut(s) 318
AoxI GGCC 4 cut(s) 270, 403, 491, 597
ApaI GGGCCC 1 cut(s) 495
AspLEI GCGC 2 cut(s) 355, 419
AspS9I GGNCC 4 cut(s) 344, 491, 492, 578
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 3 cut(s) 38, 160, 317
AvaII GGWCC 2 cut(s) 344, 578
BaeGI GKGCMC 1 cut(s) 495
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 1 cut(s) 495
BccI CCATC 2 cut(s) 17, 488
BceAI ACGGC 2 cut(s) 176, 418
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 2 cut(s) 261, 597
BlsI GCNGC 2 cut(s) 262, 598
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 2 cut(s) 344, 578
BmgT120I GGNCC 4 cut(s) 344, 491, 492, 578
BmiI GGNNCC 6 cut(s) 58, 151, 346, 387, 478, 493
BmrFI CCNGG 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 1 cut(s) 120
Bsa29I ATCGAT 1 cut(s) 132
BsaAI YACGTR 1 cut(s) 559
BsaHI GRCGYC 1 cut(s) 530
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bse1I ACTGG 3 cut(s) 440, 478, 588
BseCI ATCGAT 1 cut(s) 132
BseGI GGATG 2 cut(s) 81, 454
BseNI ACTGG 3 cut(s) 440, 478, 588
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 495
Bsh1236I CGCG 2 cut(s) 353, 572
Bsh1285I CGRYCG 1 cut(s) 576
BshFI GGCC 4 cut(s) 272, 405, 493, 599
BshNI GGYRCC 1 cut(s) 476
BshVI ATCGAT 1 cut(s) 132
BsiEI CGRYCG 1 cut(s) 576
BsiSI CCGG 2 cut(s) 119, 171
BsmAI GTCTC 1 cut(s) 138
BsmBI CGTCTC 1 cut(s) 138
BsnI GGCC 4 cut(s) 272, 405, 493, 599
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 3 cut(s) 129, 133, 323
BspACI CCGC 7 cut(s) 123, 237, 258, 261, 395, 572, 596
BspANI GGCC 4 cut(s) 272, 405, 493, 599
BspDI ATCGAT 1 cut(s) 132
BspFNI CGCG 2 cut(s) 353, 572
BspLI GGNNCC 6 cut(s) 58, 151, 346, 387, 478, 493
BspPI GGATC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 476
BsrI ACTGG 3 cut(s) 440, 478, 588
BssMI GATC 3 cut(s) 129, 133, 323
BssNAI GTATAC 1 cut(s) 540
BssNI GRCGYC 1 cut(s) 530
Bst1107I GTATAC 1 cut(s) 540
Bst4CI ACNGT 2 cut(s) 15, 65
BstACI GRCGYC 1 cut(s) 530
BstBAI YACGTR 1 cut(s) 559
BstC8I GCNNGC 2 cut(s) 570, 594
BstF5I GGATG 2 cut(s) 81, 454
BstFNI CGCG 2 cut(s) 353, 572
BstHHI GCGC 2 cut(s) 355, 419
BstKTI GATC 3 cut(s) 132, 136, 326
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 3 cut(s) 129, 133, 323
BstMCI CGRYCG 1 cut(s) 576
BstMWI GCNNNNNNNGC 3 cut(s) 269, 423, 593
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 2 cut(s) 353, 572
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BstZ17I GTATAC 1 cut(s) 540
Bsu15I ATCGAT 1 cut(s) 132
BsuRI GGCC 4 cut(s) 272, 405, 493, 599
BsuTUI ATCGAT 1 cut(s) 132
BtsCI GGATG 2 cut(s) 81, 454
BtsIMutI CAGTG 1 cut(s) 581
Cac8I GCNNGC 2 cut(s) 570, 594
CfoI GCGC 2 cut(s) 355, 419
Cfr13I GGNCC 4 cut(s) 344, 491, 492, 578
ClaI ATCGAT 1 cut(s) 132
CseI GACGC 1 cut(s) 519
Csp6I GTAC 1 cut(s) 433
CviAII CATG 4 cut(s) 268, 331, 421, 549
CviQI GTAC 1 cut(s) 433
DpnI GATC 3 cut(s) 131, 135, 325
DpnII GATC 3 cut(s) 129, 133, 323
EciI GGCGGA 2 cut(s) 247, 410
Eco24I GRGCYC 1 cut(s) 495
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 2 cut(s) 344, 578
EcoRV GATATC 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 495
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 4 cut(s) 271, 334, 424, 552
FaiI YATR 6 cut(s) 128, 269, 332, 422, 540, 550
FatI CATG 4 cut(s) 267, 330, 420, 548
FblI GTMKAC 2 cut(s) 195, 539
Fnu4HI GCNGC 2 cut(s) 261, 597
FokI GGATG 2 cut(s) 68, 461
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 2 cut(s) 261, 597
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 354, 418
GluI GCNGC 2 cut(s) 261, 597
HaeIII GGCC 4 cut(s) 272, 405, 493, 599
HapII CCGG 2 cut(s) 119, 171
HgaI GACGC 1 cut(s) 519
HhaI GCGC 2 cut(s) 355, 419
Hin1I GRCGYC 1 cut(s) 530
Hin1II CATG 4 cut(s) 271, 334, 424, 552
Hin6I GCGC 2 cut(s) 353, 417
HinP1I GCGC 2 cut(s) 353, 417
HincII GTYRAC 3 cut(s) 232, 412, 471
HindII GTYRAC 3 cut(s) 232, 412, 471
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 1 cut(s) 254
HpaI GTTAAC 1 cut(s) 412
HpaII CCGG 2 cut(s) 119, 171
HphI GGTGA 3 cut(s) 38, 160, 317
Hpy166II GTNNAC 7 cut(s) 196, 232, 412, 433, 471, 540, 562
Hpy188I TCNGA 2 cut(s) 390, 578
Hpy188III TCNNGA 1 cut(s) 442
Hpy8I GTNNAC 7 cut(s) 196, 232, 412, 433, 471, 540, 562
Hpy99I CGWCG 3 cut(s) 488, 535, 538
HpyAV CCTTC 2 cut(s) 385, 439
HpyCH4III ACNGT 2 cut(s) 15, 65
HpyCH4IV ACGT 3 cut(s) 165, 523, 558
HpyCH4V TGCA 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 423, 593
HpySE526I ACGT 3 cut(s) 165, 523, 558
Hsp92I GRCGYC 1 cut(s) 530
Hsp92II CATG 4 cut(s) 271, 334, 424, 552
HspAI GCGC 2 cut(s) 353, 417
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 3 cut(s) 129, 133, 323
LmnI GCTCC 3 cut(s) 56, 149, 377
LpnPI CCDG 5 cut(s) 132, 184, 421, 459, 569
MaeI CTAG 1 cut(s) 38
MaeII ACGT 3 cut(s) 165, 523, 558
MaeIII GTNAC 2 cut(s) 65, 166
MalI GATC 3 cut(s) 131, 135, 325
MboI GATC 3 cut(s) 129, 133, 323
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 1 cut(s) 495
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 6 cut(s) 218, 233, 275, 368, 416, 508
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 557
MspI CCGG 2 cut(s) 119, 171
MspR9I CCNGG 1 cut(s) 120
MvnI CGCG 2 cut(s) 353, 572
MwoI GCNNNNNNNGC 3 cut(s) 269, 423, 593
NciI CCSGG 1 cut(s) 120
NdeII GATC 3 cut(s) 129, 133, 323
NlaIII CATG 4 cut(s) 271, 334, 424, 552
NlaIV GGNNCC 6 cut(s) 58, 151, 346, 387, 478, 493
NmuCI GTSAC 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 557
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PkrI GCNGC 2 cut(s) 262, 598
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
Ppu21I YACGTR 1 cut(s) 559
Psp1406I AACGTT 1 cut(s) 523
PspN4I GGNNCC 6 cut(s) 58, 151, 346, 387, 478, 493
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 4 cut(s) 344, 491, 492, 578
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 2 cut(s) 261, 597
Sau3AI GATC 3 cut(s) 129, 133, 323
Sau96I GGNCC 4 cut(s) 344, 491, 492, 578
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 495
SetI ASST 7 cut(s) 168, 382, 428, 503, 526, 561, 594
SfcI CTRYAG 1 cut(s) 196
SinI GGWCC 2 cut(s) 344, 578
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SsiI CCGC 7 cut(s) 123, 237, 258, 261, 395, 572, 596
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 15, 65
TaiI ACGT 3 cut(s) 168, 526, 561
TaqI TCGA 3 cut(s) 132, 252, 513
TatI WGTACW 1 cut(s) 432
TauI GCSGC 2 cut(s) 263, 599
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 1 cut(s) 588
TseFI GTSAC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 166
TspGWI ACGGA 2 cut(s) 69, 162
TspRI CASTG 1 cut(s) 588
VpaK11BI GGWCC 2 cut(s) 344, 578
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmiI GTMKAC 2 cut(s) 195, 539
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.