pycom08g21150

Regulator of nonsense transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0017384
Physical Location & Seq
Reverse (-)
20713172 .. 20719038
5867 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21150.6

Sequence Viewer

Length: 1473 bp
ATGATGAAGGGCCAGTTGGATCGAACCAAGGTGGTGCTTCGACACTTGCCGCCTTCGATTTCGCAAGCTGCGCTTGTGGAACAAATCGACGTTTTCTTTGCTGGCCGCTACAACTGGGTTGCTTTTCGTCCTGGCAAGAGGAGCCAAAAGAATCAGTCATATTCTAGAGCCTACATTGATTTGAAGAGGCCAGATGATGTATTCGACTTTGCTGAGTTCTTTGACGGTCATCTGTTTGTTAATGAAAAGGGCAGTCAGTTTAAAGTTATTGTTGAGTATGCTCCTTCACAACGTGTTCCAAAACATTGGTCTAAGAAGGATGGTCGTGAAGGAACCATATTCAGAGATCCCGAGTATTTGGAGTTTCTTGAATTCATTGCAAAGCCTGCTGAGAATCTTCCAAGTGCAGAGACACAATTGGAGAGAAGAGAAGCAGAACGATCTGGTGCTGGAAAGGACGTTCCAATAGTTACACCATTAATGGACTTTGTTCGTCAAAAAAGGGCTGCCAAGGCTGGATCGATCATTTTTCTCGTTCGGAGGTTACCGTCTAATGGTAAAACAAGTAGAAGAGCTGGTGCATCATCTGCTCGAAGTCCTACTTTACCTATGACCAAACGGGGTTCTGAGAGGAAAAAGAATTCTGCCTCAATGTATGTTGTGAGGGATGCTAGAAAGAACACCAGTGCCAAAGACAAGTCAACATATACATTGGTACCCAAGCGCGATGATCAGCAACCTTCCGAGAAGTCTGCCATTTTGGCATCTGCAGTTGGAACTCAATTGTTTGAAGAGGAGAGTGGAGTTTTGGGTGCTGATGCTGGGAAAAAGAAAGTCTTGCTTGTGAAAGAGAAAGAGAAGGAGATATCTCATGTGCCGGCGAACATGTCACAGCAGCATAGTACATATTCTAAAAACATGGGTGGTTCAGTTGCACTGAAACAGAACCCGCGTCATCATGATAAGGGAAGGATTATCAAAAGCATACTTCTAAACAAGGATGCACGTCAAAGTCAGTCTTCTGGGATTCACTTGGAGCAGCAAATCCAGAGTTCAAGTTCAGATAGGGACAAACGACTTCCTCGGTCGCAACATGTGCAATTGATTTTAAAGGATACAAATGGAGCTCCAGATCATAAGATAGTTGGGAATGACTTGCATGGTAGTTATAGTGAGAAGCAGGAGAAACGGACGAGGAATAAGGACAGACCTGATCGTGGTGTATGGACTCCTCTTAATCGTTTAGATGGATCATCTGCAAGCGATGAGTCTTTGTCGTCTGCTTTTCAACCTGATCACTCACTCTTAGATTCTTCAGAAGGCTCCCATAAACATCATGGTCGCCGTGGAGCAACACATGGTGTTAAGGATCTTGATGGCTCTCCAGTTGCAGGTGAAGGAAAACATTCGAAGAGAGGCTATGGCTCCCATGAGAAACAAGTGTGGGTTCAGAAGTCAAGTTCTGGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
Smg4_UPF3

Protein Analysis

491

Amino Acids

54.68

Weight (kDa)

9.9

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1382
Acc36I ACCTGC 1 cut(s) 1382
Acc65I GGTACC 1 cut(s) 715
AccB1I GGYRCC 1 cut(s) 715
AccII CGCG 2 cut(s) 726, 952
AciI CCGC 3 cut(s) 50, 106, 950
AclWI GGATC 5 cut(s) 27, 341, 526, 1258, 1377
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 371, 640
AcuI CTGAAG 1 cut(s) 1299
AdeI CACNNNGTG 2 cut(s) 293, 1361
AfaI GTAC 2 cut(s) 717, 904
AfiI CCNNNNNNNGG 3 cut(s) 554, 1217, 1391
AflIII ACRYGT 3 cut(s) 292, 885, 1093
AgsI TTSAA 5 cut(s) 184, 371, 791, 1056, 1289
AjiI CACGTC 1 cut(s) 1007
AjnI CCWGG 1 cut(s) 130
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AloI GAACNNNNNNTCC 2 cut(s) 444, 476
AluBI AGCT 3 cut(s) 68, 575, 1127
AluI AGCT 3 cut(s) 68, 575, 1127
Alw21I GWGCWC 1 cut(s) 1129
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 5 cut(s) 27, 341, 526, 1258, 1377
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 3 cut(s) 10, 103, 188
ApeKI GCWGC 4 cut(s) 68, 506, 895, 1039
ApoI RAATTY 2 cut(s) 371, 640
ArsI GACNNNNNNTTYG 4 cut(s) 80, 112, 293, 325
AseI ATTAAT 1 cut(s) 479
Asp700I GAANNNNTTC 1 cut(s) 1405
Asp718I GGTACC 1 cut(s) 715
AspLEI GCGC 2 cut(s) 73, 726
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 1 cut(s) 1406
AsuII TTCGAA 1 cut(s) 1409
AvaI CYCGRG 1 cut(s) 350
BaeI ACNNNNGTAYC 2 cut(s) 699, 732
BanI GGYRCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 1129
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 1 cut(s) 1129
BbvI GCAGC 4 cut(s) 55, 493, 907, 1051
BccI CCATC 3 cut(s) 314, 1241, 1370
BceAI ACGGC 1 cut(s) 1329
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 1 cut(s) 132
BciVI GTATCC 1 cut(s) 1108
BclI TGATCA 2 cut(s) 730, 1294
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 2 cut(s) 165, 672
BfmI CTRYAG 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 1382
BfuI GTATCC 1 cut(s) 1108
BglI GCCNNNNNGGC 1 cut(s) 761
BisI GCNGC 6 cut(s) 50, 69, 106, 507, 896, 1040
BlsI GCNGC 6 cut(s) 51, 70, 107, 508, 897, 1041
Bme1390I CCNGG 1 cut(s) 132
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 1007
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 6 cut(s) 143, 334, 717, 1324, 1426, 1468
BmrFI CCNGG 1 cut(s) 132
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 5 cut(s) 590, 658, 773, 808, 991
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 1011
BpmI CTGGAG 2 cut(s) 1113, 1368
Bpu14I TTCGAA 1 cut(s) 1409
Bsa29I ATCGAT 1 cut(s) 521
BsaJI CCNNGG 4 cut(s) 27, 510, 1082, 1345
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 1217, 1391
Bse118I RCCGGY 1 cut(s) 877
Bse1I ACTGG 4 cut(s) 13, 119, 684, 1385
Bse3DI GCAATG 1 cut(s) 375
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 521
BseDI CCNNGG 4 cut(s) 27, 510, 1082, 1345
BseGI GGATG 3 cut(s) 325, 673, 1006
BseLI CCNNNNNNNGG 3 cut(s) 554, 1217, 1391
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 3 cut(s) 204, 381, 618
BseNI ACTGG 4 cut(s) 13, 119, 684, 1385
BseRI GAGGAG 3 cut(s) 154, 809, 1221
BseXI GCAGC 4 cut(s) 55, 493, 907, 1051
BseYI CCCAGC 1 cut(s) 821
BsgI GTGCAG 1 cut(s) 426
Bsh1236I CGCG 2 cut(s) 726, 952
Bsh1285I CGRYCG 1 cut(s) 1088
BshFI GGCC 3 cut(s) 12, 105, 190
BshNI GGYRCC 1 cut(s) 715
BshVI ATCGAT 1 cut(s) 521
BsiEI CGRYCG 1 cut(s) 1088
BsiHKAI GWGCWC 1 cut(s) 1129
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 1 cut(s) 878
BslFI GGGAC 1 cut(s) 1082
BslI CCNNNNNNNGG 3 cut(s) 554, 1217, 1391
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 1 cut(s) 1082
BsnI GGCC 3 cut(s) 12, 105, 190
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 1409
Bsp1286I GDGCHC 1 cut(s) 1129
BspACI CCGC 3 cut(s) 50, 106, 950
BspANI GGCC 3 cut(s) 12, 105, 190
BspCNI CTCAG 3 cut(s) 205, 382, 619
BspDI ATCGAT 1 cut(s) 521
BspFNI CGCG 2 cut(s) 726, 952
BspHI TCATGA 1 cut(s) 958
BspLI GGNNCC 6 cut(s) 143, 334, 717, 1324, 1426, 1468
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 1 cut(s) 1382
BspPI GGATC 5 cut(s) 27, 341, 526, 1258, 1377
BspQI GCTCTTC 1 cut(s) 565
BspT104I TTCGAA 1 cut(s) 1409
BspT107I GGYRCC 1 cut(s) 715
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 1 cut(s) 877
BsrI ACTGG 4 cut(s) 13, 119, 684, 1385
BssAI RCCGGY 1 cut(s) 877
BssECI CCNNGG 4 cut(s) 27, 510, 1082, 1345
BssT1I CCWWGG 2 cut(s) 27, 510
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 227, 549
Bst6I CTCTTC 5 cut(s) 179, 421, 565, 786, 1406
BstAPI GCANNNNNTGC 3 cut(s) 386, 587, 1096
BstBI TTCGAA 1 cut(s) 1409
BstC8I GCNNGC 5 cut(s) 66, 103, 387, 879, 1261
BstDEI CTNAG 5 cut(s) 213, 312, 390, 627, 1306
BstDSI CCRYGG 1 cut(s) 1345
BstEII GGTNACC 1 cut(s) 543
BstF5I GGATG 3 cut(s) 325, 673, 1006
BstFNI CGCG 2 cut(s) 726, 952
BstHHI GCGC 2 cut(s) 73, 726
BstMAI GTCTC 1 cut(s) 404
BstMCI CGRYCG 1 cut(s) 1088
BstMWI GCNNNNNNNGC 7 cut(s) 70, 141, 386, 512, 587, 761, 1096
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 2 cut(s) 889, 1097
BstPI GGTNACC 1 cut(s) 543
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 1 cut(s) 768
BstUI CGCG 2 cut(s) 726, 952
BstV1I GCAGC 4 cut(s) 55, 493, 907, 1051
BstV2I GAAGAC 1 cut(s) 1011
BstX2I RGATCY 2 cut(s) 346, 1369
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 2 cut(s) 346, 1369
Bsu15I ATCGAT 1 cut(s) 521
BsuI GTATCC 1 cut(s) 1108
BsuRI GGCC 3 cut(s) 12, 105, 190
BsuTUI ATCGAT 1 cut(s) 521
BtgI CCRYGG 1 cut(s) 1345
BtgZI GCGATG 2 cut(s) 741, 1278
BtrI CACGTC 1 cut(s) 1007
BtsCI GGATG 3 cut(s) 325, 673, 1006
BtsIMutI CAGTG 2 cut(s) 691, 935
BveI ACCTGC 1 cut(s) 1382
Cac8I GCNNGC 5 cut(s) 66, 103, 387, 879, 1261
CciI TCATGA 1 cut(s) 958
CfoI GCGC 2 cut(s) 73, 726
Cfr10I RCCGGY 1 cut(s) 877
Cfr13I GGNCC 1 cut(s) 10
ClaI ATCGAT 1 cut(s) 521
CseI GACGC 1 cut(s) 941
Csp6I GTAC 2 cut(s) 716, 903
CviAII CATG 9 cut(s) 872, 886, 919, 959, 1094, 1160, 1337, 1358, 1430
CviQI GTAC 2 cut(s) 716, 903
DdeI CTNAG 5 cut(s) 213, 312, 390, 627, 1306
DraI TTTAAA 2 cut(s) 262, 1110
DraIII CACNNNGTG 2 cut(s) 293, 1361
EaeI YGGCCR 1 cut(s) 103
Eam1104I CTCTTC 5 cut(s) 179, 421, 565, 786, 1406
EarI CTCTTC 5 cut(s) 179, 421, 565, 786, 1406
Ecl136II GAGCTC 1 cut(s) 1127
Eco130I CCWWGG 2 cut(s) 27, 510
Eco24I GRGCYC 1 cut(s) 1129
Eco32I GATATC 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 1127
Eco57I CTGAAG 1 cut(s) 1299
Eco88I CYCGRG 1 cut(s) 350
Eco91I GGTNACC 1 cut(s) 543
EcoICRI GAGCTC 1 cut(s) 1127
EcoO65I GGTNACC 1 cut(s) 543
EcoRI GAATTC 2 cut(s) 371, 640
EcoRII CCWGG 1 cut(s) 130
EcoRV GATATC 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 27, 510
EcoT38I GRGCYC 1 cut(s) 1129
ErhI CCWWGG 2 cut(s) 27, 510
FaeI CATG 9 cut(s) 875, 889, 922, 962, 1097, 1163, 1340, 1361, 1433
FaqI GGGAC 1 cut(s) 1082
FatI CATG 9 cut(s) 871, 885, 918, 958, 1093, 1159, 1336, 1357, 1429
FauI CCCGC 1 cut(s) 957
FbaI TGATCA 2 cut(s) 730, 1294
Fnu4HI GCNGC 6 cut(s) 50, 69, 106, 507, 896, 1040
FokI GGATG 3 cut(s) 332, 680, 1013
FriOI GRGCYC 1 cut(s) 1129
Fsp4HI GCNGC 6 cut(s) 50, 69, 106, 507, 896, 1040
FspBI CTAG 2 cut(s) 165, 672
GlaI GCGC 2 cut(s) 72, 725
GluI GCNGC 6 cut(s) 50, 69, 106, 507, 896, 1040
GsaI CCCAGC 1 cut(s) 825
GsuI CTGGAG 2 cut(s) 1113, 1368
HaeIII GGCC 3 cut(s) 12, 105, 190
HapII CCGG 1 cut(s) 878
HgaI GACGC 1 cut(s) 941
HhaI GCGC 2 cut(s) 73, 726
Hin1II CATG 9 cut(s) 875, 889, 922, 962, 1097, 1163, 1340, 1361, 1433
Hin6I GCGC 2 cut(s) 71, 724
HinP1I GCGC 2 cut(s) 71, 724
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HinfI GANTC 6 cut(s) 151, 394, 1027, 1228, 1268, 1310
HpaII CCGG 1 cut(s) 878
HphI GGTGA 1 cut(s) 1406
Hpy166II GTNNAC 1 cut(s) 702
Hpy188I TCNGA 7 cut(s) 344, 540, 628, 745, 1063, 1318, 1452
Hpy188III TCNNGA 9 cut(s) 165, 326, 350, 368, 959, 1048, 1130, 1373, 1470
Hpy8I GTNNAC 1 cut(s) 702
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 9 cut(s) 63, 294, 310, 323, 750, 853, 963, 1313, 1391
HpyCH4III ACNGT 2 cut(s) 227, 549
HpyCH4IV ACGT 4 cut(s) 90, 292, 459, 1006
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 141, 386, 512, 587, 761, 1096
HpyF3I CTNAG 5 cut(s) 213, 312, 390, 627, 1306
HpySE526I ACGT 4 cut(s) 90, 292, 459, 1006
Hsp92II CATG 9 cut(s) 875, 889, 922, 962, 1097, 1163, 1340, 1361, 1433
HspAI GCGC 2 cut(s) 71, 724
KpnI GGTACC 1 cut(s) 719
KroI GCCGGC 1 cut(s) 877
KroNI GCCGGC 1 cut(s) 879
Ksp22I TGATCA 2 cut(s) 730, 1294
LguI GCTCTTC 1 cut(s) 565
LmnI GCTCC 8 cut(s) 141, 286, 1036, 1124, 1132, 1328, 1349, 1430
Lsp1109I GCAGC 4 cut(s) 55, 493, 907, 1051
LweI GCATC 5 cut(s) 590, 658, 773, 808, 991
MaeI CTAG 2 cut(s) 165, 672
MaeII ACGT 4 cut(s) 90, 292, 459, 1006
MaeIII GTNAC 3 cut(s) 469, 543, 888
MboII GAAGA 8 cut(s) 196, 389, 438, 582, 803, 1011, 1305, 1423
MfeI CAATTG 3 cut(s) 416, 782, 1100
MflI RGATCY 2 cut(s) 346, 1369
MhlI GDGCHC 1 cut(s) 1129
MluCI AATT 5 cut(s) 371, 416, 640, 782, 1100
MlyI GAGTC 2 cut(s) 1222, 1277
MmeI TCCRAC 1 cut(s) 754
MroNI GCCGGC 1 cut(s) 877
MroXI GAANNNNTTC 1 cut(s) 1405
MseI TTAA 6 cut(s) 240, 261, 479, 1109, 1236, 1365
MspI CCGG 1 cut(s) 878
MspR9I CCNGG 1 cut(s) 132
MunI CAATTG 3 cut(s) 416, 782, 1100
MvaI CCWGG 1 cut(s) 132
MvnI CGCG 2 cut(s) 726, 952
MwoI GCNNNNNNNGC 7 cut(s) 70, 141, 386, 512, 587, 761, 1096
NaeI GCCGGC 1 cut(s) 879
NgoMIV GCCGGC 1 cut(s) 877
NlaIII CATG 9 cut(s) 875, 889, 922, 962, 1097, 1163, 1340, 1361, 1433
NlaIV GGNNCC 6 cut(s) 143, 334, 717, 1324, 1426, 1468
NmuCI GTSAC 1 cut(s) 888
NspI RCATGY 2 cut(s) 889, 1097
NspV TTCGAA 1 cut(s) 1409
PagI TCATGA 1 cut(s) 958
PaqCI CACCTGC 1 cut(s) 1382
PciI ACATGT 2 cut(s) 885, 1093
PciSI GCTCTTC 1 cut(s) 565
PdiI GCCGGC 1 cut(s) 879
PdmI GAANNNNTTC 1 cut(s) 1405
PfeI GAWTC 4 cut(s) 151, 394, 1027, 1310
PkrI GCNGC 6 cut(s) 51, 70, 107, 508, 897, 1041
PleI GAGTC 2 cut(s) 1222, 1276
PpsI GAGTC 2 cut(s) 1222, 1276
PscI ACATGT 2 cut(s) 885, 1093
PshBI ATTAAT 1 cut(s) 479
Psp124BI GAGCTC 1 cut(s) 1129
Psp6I CCWGG 1 cut(s) 130
PspEI GGTNACC 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 6 cut(s) 143, 334, 717, 1324, 1426, 1468
PspPI GGNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 2 cut(s) 346, 1369
RsaI GTAC 2 cut(s) 717, 904
RsaNI GTAC 2 cut(s) 716, 903
SacI GAGCTC 1 cut(s) 1129
SapI GCTCTTC 1 cut(s) 565
SaqAI TTAA 6 cut(s) 240, 261, 479, 1109, 1236, 1365
SatI GCNGC 6 cut(s) 50, 69, 106, 507, 896, 1040
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 2 cut(s) 1222, 1277
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 1129
SfaNI GCATC 5 cut(s) 590, 658, 773, 808, 991
SfcI CTRYAG 1 cut(s) 768
SfuI TTCGAA 1 cut(s) 1409
Sse9I AATT 5 cut(s) 371, 416, 640, 782, 1100
SsiI CCGC 3 cut(s) 50, 106, 950
SspMI CTAG 2 cut(s) 165, 672
SstI GAGCTC 1 cut(s) 1129
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 2 cut(s) 27, 510
TaaI ACNGT 2 cut(s) 227, 549
TaiI ACGT 4 cut(s) 93, 295, 462, 1009
TaqI TCGA 8 cut(s) 22, 40, 56, 87, 204, 521, 592, 1409
TaqII GACCGA 1 cut(s) 1074
TasI AATT 5 cut(s) 371, 416, 640, 782, 1100
TatI WGTACW 1 cut(s) 902
TauI GCSGC 2 cut(s) 52, 108
TfiI GAWTC 4 cut(s) 151, 394, 1027, 1310
Tru1I TTAA 6 cut(s) 240, 261, 479, 1109, 1236, 1365
Tru9I TTAA 6 cut(s) 240, 261, 479, 1109, 1236, 1365
TscAI CASTG 2 cut(s) 691, 942
TseFI GTSAC 1 cut(s) 888
TseI GCWGC 4 cut(s) 68, 506, 895, 1039
Tsp45I GTSAC 1 cut(s) 888
TspDTI ATGAA 3 cut(s) 20, 258, 364
TspGWI ACGGA 1 cut(s) 1204
TspRI CASTG 2 cut(s) 691, 942
VspI ATTAAT 1 cut(s) 479
XapI RAATTY 2 cut(s) 371, 640
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 2 cut(s) 889, 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 1334
XmnI GAANNNNTTC 1 cut(s) 1405
XspI CTAG 2 cut(s) 165, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.