pycom09g06480

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0021259
Physical Location & Seq
Reverse (-)
4761194 .. 4764211
3018 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06480.1

Sequence Viewer

Length: 285 bp
ATGGATAAACCGAGGCCGGTAGATTTCAACTGCCTCACAACCGCCGCCAGAGAGGTCAGGTTGTCCAGAGCACTACCTCTTCCTCCGCCAACACCGCTGTGTCAGATGTTAAGCTGCAGCTCCAGGAGGACCACAGCAATGGCTTCAATCCCATCCTCCACTTTTGCTTCTGGATCGACTTGCGAAAGAACAGAGGAGAGCACAAGCATTATCAATTTATTTGCTATAGAGTTTGTCAGGGAGAAAGTTCGTGAACAAAAGAAATCTAATCGAGAGGCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

95

Amino Acids

10.4

Weight (kDa)

9.2

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021259)

Species Orthologous Gene IDs
pyrus_communis pycom04g01120 pycom05g10160 pycom05g25620 pycom09g06480 pycom10g20690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 42, 45, 86, 95
AclWI GGATC 1 cut(s) 181
AgsI TTSAA 2 cut(s) 28, 147
AjnI CCWGG 1 cut(s) 122
AleI CACNNNNGTG 1 cut(s) 97
AluBI AGCT 2 cut(s) 114, 120
AluI AGCT 2 cut(s) 114, 120
Alw21I GWGCWC 2 cut(s) 73, 203
AlwI GGATC 1 cut(s) 181
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 2 cut(s) 114, 117
AspS9I GGNCC 1 cut(s) 129
AvaII GGWCC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 73, 203
BbvI GCAGC 2 cut(s) 101, 129
BccI CCATC 1 cut(s) 160
BciT130I CCWGG 1 cut(s) 124
BfmI CTRYAG 2 cut(s) 115, 225
BisI GCNGC 3 cut(s) 45, 115, 118
BlsI GCNGC 3 cut(s) 46, 116, 119
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 11
Bse118I RCCGGY 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 1 cut(s) 152
BseMI GCAATG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 101, 129
BshFI GGCC 1 cut(s) 16
BsiHKAI GWGCWC 2 cut(s) 73, 203
BsiSI CCGG 1 cut(s) 17
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 73, 203
Bsp143I GATC 1 cut(s) 173
BspACI CCGC 4 cut(s) 42, 45, 86, 95
BspANI GGCC 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 124
Bst6I CTCTTC 1 cut(s) 84
BstF5I GGATG 1 cut(s) 152
BstKTI GATC 1 cut(s) 176
BstMBI GATC 1 cut(s) 173
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNI CCWGG 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 122
BstSFI CTRYAG 2 cut(s) 115, 225
BstV1I GCAGC 2 cut(s) 101, 129
BstXI CCANNNNNNTGG 1 cut(s) 139
BsuRI GGCC 1 cut(s) 16
BtsCI GGATG 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 129
CviJI RGCY 5 cut(s) 16, 114, 120, 143, 278
CviKI_1 RGCY 5 cut(s) 16, 114, 120, 143, 278
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
EciI GGCGGA 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 122
FaiI YATR 1 cut(s) 227
Fnu4HI GCNGC 3 cut(s) 45, 115, 118
FokI GGATG 1 cut(s) 139
Fsp4HI GCNGC 3 cut(s) 45, 115, 118
GluI GCNGC 3 cut(s) 45, 115, 118
GsuI CTGGAG 1 cut(s) 106
HaeIII GGCC 1 cut(s) 16
HapII CCGG 1 cut(s) 17
HpaII CCGG 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 4 cut(s) 66, 171, 251, 272
Hpy8I GTNNAC 1 cut(s) 254
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 173
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 9 cut(s) 30, 43, 61, 79, 109, 136, 156, 223, 264
Lsp1109I GCAGC 2 cut(s) 101, 129
MalI GATC 1 cut(s) 175
MboI GATC 1 cut(s) 173
MboII GAAGA 1 cut(s) 71
MhlI GDGCHC 2 cut(s) 73, 203
MluCI AATT 1 cut(s) 214
MnlI CCTC 9 cut(s) 6, 44, 46, 87, 93, 120, 166, 187, 268
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 2 cut(s) 97, 137
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 94
NdeII GATC 1 cut(s) 173
OliI CACNNNNGTG 1 cut(s) 97
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 3 cut(s) 46, 116, 119
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 119
RseI CAYNNNNRTG 2 cut(s) 97, 137
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 3 cut(s) 45, 115, 118
Sau3AI GATC 1 cut(s) 173
Sau96I GGNCC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 2 cut(s) 73, 203
SetI ASST 5 cut(s) 57, 62, 79, 116, 122
SfcI CTRYAG 2 cut(s) 115, 225
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 2 cut(s) 97, 137
Sse9I AATT 1 cut(s) 214
SsiI CCGC 4 cut(s) 42, 45, 86, 95
StyD4I CCNGG 1 cut(s) 122
TaqI TCGA 2 cut(s) 176, 271
TasI AATT 1 cut(s) 214
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TseI GCWGC 2 cut(s) 114, 117
VpaK11BI GGWCC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.