pycom10g20690

aminoacylase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0021259
Physical Location & Seq
Reverse (-)
23530538 .. 23533832
3295 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20690.5

Sequence Viewer

Length: 390 bp
ATGGATAAACCGAGGCCGGTAGATTTCAACTGCCTCACAACCGCCGCCAGAGAGGTCAGATTGTCCAGAGCGCTGCCTTTTCCTCCGCCAACACCGTTGTATCAGATGTTAAGCTGCACCTCCAGGAGGACCACAGCAATGGCTTCAATCCCATCCTCCACTTTTGCTTCTGGATCGACTTGGGAAAGAACAGAGGAGAGCACAAGCATTATCAATTTATTTGCTATAGAGTTTGTCAGGGAGAAAGTTCGTGAACGAAAGAAATCTAATCGAGAGGGTTGGATGAAAAGAAACACCCATCAGAGCTTTCAACAACTCGAAGGTTTTATAACATGTAAACAGGGAGTACATACTTTGTATGCCATGGATAGGGAATGGATACGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.99

Weight (kDa)

9.84

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021259)

Species Orthologous Gene IDs
pyrus_communis pycom04g01120 pycom05g10160 pycom05g25620 pycom09g06480 pycom10g20690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 329
AciI CCGC 3 cut(s) 42, 45, 86
AclWI GGATC 1 cut(s) 181
AfaI GTAC 1 cut(s) 348
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 2 cut(s) 126, 369
AflIII ACRYGT 1 cut(s) 332
AgsI TTSAA 3 cut(s) 28, 147, 311
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 2 cut(s) 114, 306
AluI AGCT 2 cut(s) 114, 306
Alw21I GWGCWC 1 cut(s) 203
AlwI GGATC 1 cut(s) 181
Aor51HI AGCGCT 1 cut(s) 72
AoxI GGCC 1 cut(s) 14
ApeKI GCWGC 2 cut(s) 73, 114
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 129
AvaII GGWCC 1 cut(s) 129
BaeI ACNNNNGTAYC 2 cut(s) 83, 116
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 2 cut(s) 60, 101
BccI CCATC 2 cut(s) 160, 306
BciT130I CCWGG 1 cut(s) 124
BciVI GTATCC 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 225
BfoI RGCGCY 1 cut(s) 74
BfuI GTATCC 1 cut(s) 372
BisI GCNGC 3 cut(s) 45, 74, 115
BlsI GCNGC 3 cut(s) 46, 75, 116
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 106
BsaAI YACGTR 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 11, 363
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 369
Bse118I RCCGGY 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 144
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 11, 363
BseGI GGATG 2 cut(s) 152, 288
BseLI CCNNNNNNNGG 2 cut(s) 126, 369
BseMI GCAATG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 60, 101
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 1 cut(s) 16
BsiHKAI GWGCWC 1 cut(s) 203
BsiSI CCGG 1 cut(s) 17
BslI CCNNNNNNNGG 2 cut(s) 126, 369
BsnI GGCC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 1 cut(s) 173
Bsp19I CCATGG 1 cut(s) 363
BspACI CCGC 3 cut(s) 42, 45, 86
BspANI GGCC 1 cut(s) 16
BspPI GGATC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 144
BsrFI RCCGGY 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 2 cut(s) 11, 363
BssMI GATC 1 cut(s) 173
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 383
BstDSI CCRYGG 1 cut(s) 363
BstENI CCTNNNNNAGG 1 cut(s) 124
BstF5I GGATG 2 cut(s) 152, 288
BstH2I RGCGCY 1 cut(s) 74
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 1 cut(s) 176
BstMBI GATC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 122
BstSFI CTRYAG 1 cut(s) 225
BstSNI TACGTA 1 cut(s) 383
BstV1I GCAGC 2 cut(s) 60, 101
BstXI CCANNNNNNTGG 1 cut(s) 139
BsuI GTATCC 1 cut(s) 372
BsuRI GGCC 1 cut(s) 16
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 2 cut(s) 152, 288
CfoI GCGC 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 129
Csp6I GTAC 1 cut(s) 347
CviAII CATG 2 cut(s) 333, 364
CviJI RGCY 4 cut(s) 16, 114, 143, 306
CviKI_1 RGCY 4 cut(s) 16, 114, 143, 306
CviQI GTAC 1 cut(s) 347
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
EciI GGCGGA 1 cut(s) 75
Eco105I TACGTA 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 129
Eco47III AGCGCT 1 cut(s) 72
EcoNI CCTNNNNNAGG 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 2 cut(s) 336, 367
FaiI YATR 6 cut(s) 227, 329, 334, 351, 360, 365
FatI CATG 2 cut(s) 332, 363
Fnu4HI GCNGC 3 cut(s) 45, 74, 115
FokI GGATG 2 cut(s) 139, 295
Fsp4HI GCNGC 3 cut(s) 45, 74, 115
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 45, 74, 115
GsuI CTGGAG 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 74
HaeIII GGCC 1 cut(s) 16
HapII CCGG 1 cut(s) 17
HhaI GCGC 1 cut(s) 73
Hin1II CATG 2 cut(s) 336, 367
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HpaII CCGG 1 cut(s) 17
Hpy166II GTNNAC 2 cut(s) 254, 338
Hpy188I TCNGA 3 cut(s) 59, 105, 303
Hpy188III TCNNGA 4 cut(s) 66, 171, 251, 272
Hpy8I GTNNAC 2 cut(s) 254, 338
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 96
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 2 cut(s) 336, 367
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 1 cut(s) 173
LpnPI CCDG 8 cut(s) 30, 61, 79, 109, 136, 156, 223, 326
Lsp1109I GCAGC 2 cut(s) 60, 101
MaeII ACGT 1 cut(s) 382
MalI GATC 1 cut(s) 175
MboI GATC 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 2 cut(s) 214, 385
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 9 cut(s) 6, 44, 46, 93, 120, 130, 166, 187, 268
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 1 cut(s) 17
MspR9I CCNGG 1 cut(s) 124
MvaI CCWGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 363
NdeII GATC 1 cut(s) 173
NlaIII CATG 2 cut(s) 336, 367
NspI RCATGY 1 cut(s) 336
PciI ACATGT 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 122
PkrI GCNGC 3 cut(s) 46, 75, 116
Ppu21I YACGTR 1 cut(s) 383
PscI ACATGT 1 cut(s) 332
PsiI TTATAA 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspPI GGNCC 1 cut(s) 129
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 3 cut(s) 45, 74, 115
Sau3AI GATC 1 cut(s) 173
Sau96I GGNCC 1 cut(s) 129
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 1 cut(s) 203
SetI ASST 6 cut(s) 57, 116, 122, 308, 325, 385
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 137
SnaBI TACGTA 1 cut(s) 383
Sse9I AATT 2 cut(s) 214, 385
SsiI CCGC 3 cut(s) 42, 45, 86
StyD4I CCNGG 1 cut(s) 122
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 96
TaiI ACGT 1 cut(s) 385
TaqI TCGA 3 cut(s) 176, 271, 318
TasI AATT 2 cut(s) 214, 385
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 47
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TseI GCWGC 2 cut(s) 73, 114
TspDTI ATGAA 1 cut(s) 299
VpaK11BI GGWCC 1 cut(s) 129
XagI CCTNNNNNAGG 1 cut(s) 124
XceI RCATGY 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.