pycom09g06970

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0021272
Physical Location & Seq
Forward (+)
5159651 .. 5160191
541 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
ATGGCAAAAGATTTTACGGGGAAGACAGATGATGATTTATTTAAAAAGATCGAGAGTGATGATTATATGTACTCAGCGGTAATTGAATGCTATAAGACACTCACGGATATTATAAATGACCTTTTGGAAGATGAAGCAGATAAGATGATTGTAAACAGGATATGCTCTAAAGTACACAGTAGCATACAGCAGCATAGATTTTTGACTAATTTCCGCATGAGTGGGCTGCCATTTCTTAGCGAGCGGTTGGAGAAATTTCTAAAGCTCTTGACGCAGACGAGAATGATGAAAATTTCATGCGTCAGATAA

Protein Analysis

103

Amino Acids

12.01

Weight (kDa)

6.72

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CALS1 PF25968 1 - 94 1.9e-37 Callose synthase 1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021272)

Species Orthologous Gene IDs
pyrus_communis pycom09g06970
rosa_multiflora Rmu_sc0005378.1_g000035
rosa_samantha Rh6AG329000 Rh7AG299100 Rh7DG422600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 244
AciI CCGC 3 cut(s) 77, 214, 244
AcsI RAATTY 2 cut(s) 254, 291
AfaI GTAC 2 cut(s) 71, 174
AgsI TTSAA 1 cut(s) 86
AluBI AGCT 1 cut(s) 265
AluI AGCT 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 190, 226
ApoI RAATTY 2 cut(s) 254, 291
BbsI GAAGAC 1 cut(s) 29
BbvI GCAGC 2 cut(s) 202, 213
BisI GCNGC 2 cut(s) 191, 227
BlsI GCNGC 2 cut(s) 192, 228
BpiI GAAGAC 1 cut(s) 29
BseMII CTCAG 1 cut(s) 87
BseXI GCAGC 2 cut(s) 202, 213
BsmI GAATGC 1 cut(s) 92
Bsp143I GATC 1 cut(s) 48
BspACI CCGC 3 cut(s) 77, 214, 244
BspCNI CTCAG 1 cut(s) 86
BsrBI CCGCTC 1 cut(s) 244
BssMI GATC 1 cut(s) 48
Bst4CI ACNGT 1 cut(s) 179
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 73, 236
BstKTI GATC 1 cut(s) 51
BstMBI GATC 1 cut(s) 48
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstV1I GCAGC 2 cut(s) 202, 213
BstV2I GAAGAC 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 242
CseI GACGC 2 cut(s) 280, 289
Csp6I GTAC 2 cut(s) 70, 173
CviAII CATG 2 cut(s) 217, 297
CviJI RGCY 2 cut(s) 226, 265
CviKI_1 RGCY 2 cut(s) 226, 265
CviQI GTAC 2 cut(s) 70, 173
DdeI CTNAG 2 cut(s) 73, 236
DpnI GATC 1 cut(s) 50
DpnII GATC 1 cut(s) 48
DraI TTTAAA 1 cut(s) 43
FaeI CATG 2 cut(s) 220, 300
FaiI YATR 9 cut(s) 66, 68, 93, 113, 163, 185, 195, 218, 298
FatI CATG 2 cut(s) 216, 296
Fnu4HI GCNGC 2 cut(s) 191, 227
Fsp4HI GCNGC 2 cut(s) 191, 227
GluI GCNGC 2 cut(s) 191, 227
HgaI GACGC 2 cut(s) 280, 289
Hin1II CATG 2 cut(s) 220, 300
Hpy166II GTNNAC 2 cut(s) 154, 175
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 2 cut(s) 52, 268
Hpy8I GTNNAC 2 cut(s) 154, 175
HpyCH4III ACNGT 1 cut(s) 179
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 2 cut(s) 73, 236
Hsp92II CATG 2 cut(s) 220, 300
Kzo9I GATC 1 cut(s) 48
LpnPI CCDG 1 cut(s) 142
Lsp1109I GCAGC 2 cut(s) 202, 213
MalI GATC 1 cut(s) 50
MbiI CCGCTC 1 cut(s) 244
MboI GATC 1 cut(s) 48
MboII GAAGA 2 cut(s) 34, 140
MluCI AATT 4 cut(s) 81, 208, 254, 291
MmeI TCCRAC 1 cut(s) 228
MseI TTAA 1 cut(s) 42
MspA1I CMGCKG 1 cut(s) 77
Mva1269I GAATGC 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 271
NdeII GATC 1 cut(s) 48
NlaIII CATG 2 cut(s) 220, 300
PctI GAATGC 1 cut(s) 92
PkrI GCNGC 2 cut(s) 192, 228
PsiI TTATAA 1 cut(s) 113
RsaI GTAC 2 cut(s) 71, 174
RsaNI GTAC 2 cut(s) 70, 173
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 2 cut(s) 191, 227
Sau3AI GATC 1 cut(s) 48
SetI ASST 2 cut(s) 123, 267
SgeI CNNG 8 cut(s) 30, 64, 115, 169, 229, 253, 280, 291
Sse9I AATT 4 cut(s) 81, 208, 254, 291
SsiI CCGC 3 cut(s) 77, 214, 244
TaaI ACNGT 1 cut(s) 179
TaqI TCGA 1 cut(s) 51
TasI AATT 4 cut(s) 81, 208, 254, 291
TatI WGTACW 2 cut(s) 69, 172
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TseI GCWGC 2 cut(s) 190, 226
TspDTI ATGAA 3 cut(s) 147, 285, 302
TspGWI ACGGA 1 cut(s) 119
XapI RAATTY 2 cut(s) 254, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.