Rh7AG299100

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0021272
Physical Location & Seq
Forward (+)
32732120 .. 32737436
5317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG299100.1

Sequence Viewer

Length: 768 bp
ATGATTGGCATGAGCTTTTTCCGAAAGTTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGAACTTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATTGAGGAATGACCAAAAGAGAAGAAGAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAGACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTGAGGACTTTAGGATGATTAAAGTTCCAGATCTTTGGGCAGTGAACCTTGTGGACAAAATGTTCAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

255

Amino Acids

29.65

Weight (kDa)

9.53

Isoelectric Point (pI)

55.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CALS1 PF25968 152 - 244 7.8e-31 Callose synthase 1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021272)

Species Orthologous Gene IDs
pyrus_communis pycom09g06970
rosa_multiflora Rmu_sc0005378.1_g000035
rosa_samantha Rh6AG329000 Rh7AG299100 Rh7DG422600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 213
AclWI GGATC 1 cut(s) 633
AcoI YGGCCR 1 cut(s) 452
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 408
AfaI GTAC 4 cut(s) 83, 557, 563, 599
AfiI CCNNNNNNNGG 1 cut(s) 246
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 6 cut(s) 220, 483, 595, 608, 668, 760
AleI CACNNNNGTG 1 cut(s) 159
AloI GAACNNNNNNTCC 1 cut(s) 740
AluBI AGCT 2 cut(s) 15, 397
AluI AGCT 2 cut(s) 15, 397
AlwI GGATC 1 cut(s) 633
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 287
AsuHPI GGTGA 2 cut(s) 146, 173
AsuII TTCGAA 1 cut(s) 169
BalI TGGCCA 1 cut(s) 454
BanII GRGCYC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 566, 598
BbsI GAAGAC 3 cut(s) 414, 544, 683
BbvI GCAGC 1 cut(s) 40
BglII AGATCT 1 cut(s) 724
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
BmiI GGNNCC 2 cut(s) 66, 347
BmsI GCATC 2 cut(s) 160, 684
BpiI GAAGAC 3 cut(s) 414, 544, 683
Bpu14I TTCGAA 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 716
BsaJI CCNNGG 1 cut(s) 157
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 157
BseGI GGATG 3 cut(s) 526, 600, 714
BseLI CCNNNNNNNGG 1 cut(s) 246
BseMII CTCAG 2 cut(s) 110, 482
BseNI ACTGG 1 cut(s) 455
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 1 cut(s) 40
BshFI GGCC 1 cut(s) 454
BsiSI CCGG 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 246
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 1 cut(s) 454
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 3 cut(s) 625, 683, 724
BspACI CCGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 2 cut(s) 109, 483
BspLI GGNNCC 2 cut(s) 66, 347
BspPI GGATC 1 cut(s) 633
BspT104I TTCGAA 1 cut(s) 169
BsrI ACTGG 1 cut(s) 455
BssECI CCNNGG 1 cut(s) 157
BssMI GATC 3 cut(s) 625, 683, 724
BssT1I CCWWGG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 566
Bst6I CTCTTC 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 169
BstDEI CTNAG 2 cut(s) 96, 491
BstENI CCTNNNNNAGG 1 cut(s) 244
BstF5I GGATG 3 cut(s) 526, 600, 714
BstKTI GATC 3 cut(s) 628, 686, 727
BstMBI GATC 3 cut(s) 625, 683, 724
BstMWI GCNNNNNNNGC 2 cut(s) 53, 62
BstNSI RCATGY 1 cut(s) 562
BstV1I GCAGC 1 cut(s) 40
BstV2I GAAGAC 3 cut(s) 414, 544, 683
BstX2I RGATCY 2 cut(s) 625, 724
BstXI CCANNNNNNTGG 1 cut(s) 729
BstYI RGATCY 2 cut(s) 625, 724
BsuRI GGCC 1 cut(s) 454
BtsCI GGATG 3 cut(s) 526, 600, 714
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 741
Csp6I GTAC 4 cut(s) 82, 556, 562, 598
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 2 cut(s) 10, 559
CviJI RGCY 4 cut(s) 15, 65, 397, 454
CviKI_1 RGCY 4 cut(s) 15, 65, 397, 454
CviQI GTAC 4 cut(s) 82, 556, 562, 598
DdeI CTNAG 2 cut(s) 96, 491
DpnI GATC 3 cut(s) 627, 685, 726
DpnII GATC 3 cut(s) 625, 683, 724
EaeI YGGCCR 1 cut(s) 452
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 408
EcoNI CCTNNNNNAGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 2 cut(s) 13, 562
FalI AAGNNNNNCTT 2 cut(s) 320, 352
FatI CATG 2 cut(s) 9, 558
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 3 cut(s) 533, 587, 721
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 454
HapII CCGG 1 cut(s) 438
Hin1II CATG 2 cut(s) 13, 562
HinfI GANTC 3 cut(s) 135, 166, 587
HpaII CCGG 1 cut(s) 438
HphI GGTGA 2 cut(s) 146, 173
Hpy166II GTNNAC 3 cut(s) 82, 739, 748
Hpy188I TCNGA 5 cut(s) 23, 99, 293, 427, 536
Hpy188III TCNNGA 4 cut(s) 629, 695, 722, 760
Hpy8I GTNNAC 3 cut(s) 82, 739, 748
HpyAV CCTTC 2 cut(s) 219, 568
HpyCH4III ACNGT 1 cut(s) 566
HpyCH4V TGCA 4 cut(s) 120, 151, 470, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 62
HpyF3I CTNAG 2 cut(s) 96, 491
Hsp92II CATG 2 cut(s) 13, 562
Kzo9I GATC 3 cut(s) 625, 683, 724
LmnI GCTCC 2 cut(s) 70, 394
LpnPI CCDG 7 cut(s) 27, 376, 383, 451, 468, 503, 735
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 2 cut(s) 160, 684
MalI GATC 3 cut(s) 627, 685, 726
MboI GATC 3 cut(s) 625, 683, 724
MboII GAAGA 7 cut(s) 276, 279, 414, 455, 549, 680, 683
MflI RGATCY 2 cut(s) 625, 724
MhlI GDGCHC 1 cut(s) 67
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 5 cut(s) 69, 287, 351, 428, 663
MluNI TGGCCA 1 cut(s) 454
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 9 cut(s) 180, 240, 250, 263, 367, 510, 623, 630, 691
Mox20I TGGCCA 1 cut(s) 454
MscI TGGCCA 1 cut(s) 454
MseI TTAA 4 cut(s) 30, 404, 543, 714
MslI CAYNNNNRTG 1 cut(s) 159
Msp20I TGGCCA 1 cut(s) 454
MspI CCGG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 53, 62
NdeII GATC 3 cut(s) 625, 683, 724
NlaIII CATG 2 cut(s) 13, 562
NlaIV GGNNCC 2 cut(s) 66, 347
NspI RCATGY 1 cut(s) 562
NspV TTCGAA 1 cut(s) 169
OliI CACNNNNGTG 1 cut(s) 159
PciI ACATGT 1 cut(s) 558
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 166, 587
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PscI ACATGT 1 cut(s) 558
PspN4I GGNNCC 2 cut(s) 66, 347
PsuI RGATCY 2 cut(s) 625, 724
RsaI GTAC 4 cut(s) 83, 557, 563, 599
RsaNI GTAC 4 cut(s) 82, 556, 562, 598
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 4 cut(s) 30, 404, 543, 714
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 3 cut(s) 625, 683, 724
SchI GAGTC 1 cut(s) 144
SduI GDGCHC 1 cut(s) 67
SetI ASST 6 cut(s) 17, 159, 274, 300, 399, 744
SfaNI GCATC 2 cut(s) 160, 684
SfuI TTCGAA 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 695
SmoI CTYRAG 1 cut(s) 695
Sse9I AATT 5 cut(s) 69, 287, 351, 428, 663
SsiI CCGC 1 cut(s) 213
SspI AATATT 1 cut(s) 223
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 566
TaqI TCGA 3 cut(s) 169, 367, 630
TasI AATT 5 cut(s) 69, 287, 351, 428, 663
TatI WGTACW 4 cut(s) 81, 555, 561, 597
TfiI GAWTC 2 cut(s) 166, 587
Tru1I TTAA 4 cut(s) 30, 404, 543, 714
Tru9I TTAA 4 cut(s) 30, 404, 543, 714
TscAI CASTG 1 cut(s) 741
TseI GCWGC 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 537
TspRI CASTG 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 244
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.