Rh6AG329000

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0021272
Physical Location & Seq
Reverse (-)
53088568 .. 53091142
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG329000.1

Sequence Viewer

Length: 945 bp
ATGAGGACCTTGCAGACCCATAACCTTTCTAGTTTCTATGGATGTGGTTTTAAATTGGTGGGTATTGTTCAGCAAAGGCAATTGGTATTTGGTGAAGCCAAGCCAGAGAGACACTTTTATTTGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCCAATGTATGATTGGCCTGAGCTTTTTCCGAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGAACTTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATTGAGGAATGACCAAAAGAGAAGAAGAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAGACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTGAGGACTTTAGGATGATTAAAGTTCCAGATCTTTGGGCAGTGAACCTTGTGGACAAAATGTTCAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

314

Amino Acids

36.48

Weight (kDa)

9.48

Isoelectric Point (pI)

54.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CALS1 PF25968 211 - 303 1.2e-30 Callose synthase 1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021272)

Species Orthologous Gene IDs
pyrus_communis pycom09g06970
rosa_multiflora Rmu_sc0005378.1_g000035
rosa_samantha Rh6AG329000 Rh7AG299100 Rh7DG422600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 390
AclWI GGATC 1 cut(s) 810
AcoI YGGCCR 1 cut(s) 629
AcsI RAATTY 1 cut(s) 464
AcuI CTGAAG 1 cut(s) 585
AfaI GTAC 4 cut(s) 260, 734, 740, 776
AfiI CCNNNNNNNGG 1 cut(s) 423
AflIII ACRYGT 1 cut(s) 735
AgsI TTSAA 6 cut(s) 397, 660, 772, 785, 845, 937
AleI CACNNNNGTG 1 cut(s) 336
AloI GAACNNNNNNTCC 1 cut(s) 917
AluBI AGCT 2 cut(s) 193, 574
AluI AGCT 2 cut(s) 193, 574
Alw26I GTCTC 1 cut(s) 103
AlwI GGATC 1 cut(s) 810
AoxI GGCC 2 cut(s) 185, 629
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 464
AspS9I GGNCC 1 cut(s) 6
AsuHPI GGTGA 3 cut(s) 104, 323, 350
AsuII TTCGAA 1 cut(s) 346
AvaII GGWCC 1 cut(s) 6
BalI TGGCCA 1 cut(s) 631
BanII GRGCYC 1 cut(s) 244
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbsI GAAGAC 3 cut(s) 591, 721, 860
BbvI GCAGC 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 1 cut(s) 30
BglII AGATCT 1 cut(s) 901
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 243, 524
BmsI GCATC 2 cut(s) 337, 861
BpiI GAAGAC 3 cut(s) 591, 721, 860
Bpu10I CCTNAGC 1 cut(s) 189
Bpu14I TTCGAA 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 893
BsaJI CCNNGG 1 cut(s) 334
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse1I ACTGG 1 cut(s) 632
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 4 cut(s) 47, 703, 777, 891
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMII CTCAG 3 cut(s) 180, 287, 659
BseNI ACTGG 1 cut(s) 632
BseRI GAGGAG 1 cut(s) 379
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 2 cut(s) 187, 631
BsiSI CCGG 1 cut(s) 615
BslI CCNNNNNNNGG 1 cut(s) 423
BsmAI GTCTC 1 cut(s) 103
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 2 cut(s) 187, 631
Bsp119I TTCGAA 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 4 cut(s) 153, 802, 860, 901
BspACI CCGC 1 cut(s) 390
BspANI GGCC 2 cut(s) 187, 631
BspCNI CTCAG 3 cut(s) 181, 286, 660
BspHI TCATGA 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 243, 524
BspPI GGATC 1 cut(s) 810
BspT104I TTCGAA 1 cut(s) 346
BsrI ACTGG 1 cut(s) 632
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 4 cut(s) 153, 802, 860, 901
BssT1I CCWWGG 1 cut(s) 334
Bst4CI ACNGT 1 cut(s) 743
Bst6I CTCTTC 1 cut(s) 439
BstBI TTCGAA 1 cut(s) 346
BstDEI CTNAG 3 cut(s) 189, 273, 668
BstENI CCTNNNNNAGG 1 cut(s) 421
BstF5I GGATG 4 cut(s) 47, 703, 777, 891
BstKTI GATC 4 cut(s) 156, 805, 863, 904
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 4 cut(s) 153, 802, 860, 901
BstMWI GCNNNNNNNGC 3 cut(s) 148, 230, 239
BstNSI RCATGY 1 cut(s) 739
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 3 cut(s) 591, 721, 860
BstX2I RGATCY 2 cut(s) 802, 901
BstXI CCANNNNNNTGG 1 cut(s) 906
BstYI RGATCY 2 cut(s) 802, 901
BsuRI GGCC 2 cut(s) 187, 631
BtsCI GGATG 4 cut(s) 47, 703, 777, 891
BtsI GCAGTG 1 cut(s) 918
BtsIMutI CAGTG 1 cut(s) 918
CciI TCATGA 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 6
Csp6I GTAC 4 cut(s) 259, 733, 739, 775
CspCI CAANNNNNGTGG 2 cut(s) 402, 437
CviAII CATG 2 cut(s) 157, 736
CviJI RGCY 8 cut(s) 98, 103, 142, 187, 193, 242, 574, 631
CviKI_1 RGCY 8 cut(s) 98, 103, 142, 187, 193, 242, 574, 631
CviQI GTAC 4 cut(s) 259, 733, 739, 775
DdeI CTNAG 3 cut(s) 189, 273, 668
DpnI GATC 4 cut(s) 155, 804, 862, 903
DpnII GATC 4 cut(s) 153, 802, 860, 901
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 629
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 421
EcoO109I RGGNCCY 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 2 cut(s) 160, 739
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FatI CATG 2 cut(s) 156, 735
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 4 cut(s) 54, 710, 764, 898
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 1 cut(s) 30
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 187, 631
HapII CCGG 1 cut(s) 615
Hin1II CATG 2 cut(s) 160, 739
HinfI GANTC 3 cut(s) 312, 343, 764
HpaII CCGG 1 cut(s) 615
HphI GGTGA 3 cut(s) 104, 323, 350
Hpy166II GTNNAC 3 cut(s) 259, 916, 925
Hpy188I TCNGA 5 cut(s) 201, 276, 470, 604, 713
Hpy188III TCNNGA 5 cut(s) 157, 806, 872, 899, 937
Hpy8I GTNNAC 3 cut(s) 259, 916, 925
HpyAV CCTTC 2 cut(s) 396, 745
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4V TGCA 5 cut(s) 13, 297, 328, 647, 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 230, 239
HpyF3I CTNAG 3 cut(s) 189, 273, 668
Hsp92II CATG 2 cut(s) 160, 739
Kzo9I GATC 4 cut(s) 153, 802, 860, 901
LmnI GCTCC 2 cut(s) 247, 571
LpnPI CCDG 9 cut(s) 117, 201, 204, 553, 560, 628, 645, 680, 912
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 2 cut(s) 337, 861
MaeI CTAG 1 cut(s) 30
MalI GATC 4 cut(s) 155, 804, 862, 903
MboI GATC 4 cut(s) 153, 802, 860, 901
MboII GAAGA 7 cut(s) 453, 456, 591, 632, 726, 857, 860
MfeI CAATTG 1 cut(s) 80
MflI RGATCY 2 cut(s) 802, 901
MhlI GDGCHC 1 cut(s) 244
MlsI TGGCCA 1 cut(s) 631
MluCI AATT 8 cut(s) 53, 80, 162, 246, 464, 528, 605, 840
MluNI TGGCCA 1 cut(s) 631
MlyI GAGTC 1 cut(s) 321
MnlI CCTC 9 cut(s) 357, 417, 427, 440, 544, 687, 800, 807, 868
Mox20I TGGCCA 1 cut(s) 631
MscI TGGCCA 1 cut(s) 631
MseI TTAA 5 cut(s) 51, 207, 581, 720, 891
MslI CAYNNNNRTG 1 cut(s) 336
Msp20I TGGCCA 1 cut(s) 631
MspI CCGG 1 cut(s) 615
MunI CAATTG 1 cut(s) 80
Mva1269I GAATGC 1 cut(s) 392
MwoI GCNNNNNNNGC 3 cut(s) 148, 230, 239
NdeII GATC 4 cut(s) 153, 802, 860, 901
NlaIII CATG 2 cut(s) 160, 739
NlaIV GGNNCC 2 cut(s) 243, 524
NspI RCATGY 1 cut(s) 739
NspV TTCGAA 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 336
PagI TCATGA 1 cut(s) 156
PciI ACATGT 1 cut(s) 735
PctI GAATGC 1 cut(s) 392
PfeI GAWTC 2 cut(s) 343, 764
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 320
PpsI GAGTC 1 cut(s) 320
PpuMI RGGWCCY 1 cut(s) 6
PscI ACATGT 1 cut(s) 735
Psp5II RGGWCCY 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 243, 524
PspPI GGNCC 1 cut(s) 6
PspPPI RGGWCCY 1 cut(s) 6
PsuI RGATCY 2 cut(s) 802, 901
RsaI GTAC 4 cut(s) 260, 734, 740, 776
RsaNI GTAC 4 cut(s) 259, 733, 739, 775
RseI CAYNNNNRTG 1 cut(s) 336
SaqAI TTAA 5 cut(s) 51, 207, 581, 720, 891
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 4 cut(s) 153, 802, 860, 901
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 321
SduI GDGCHC 1 cut(s) 244
SetI ASST 8 cut(s) 11, 27, 195, 336, 451, 477, 576, 921
SfaNI GCATC 2 cut(s) 337, 861
SfuI TTCGAA 1 cut(s) 346
SinI GGWCC 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
Sse9I AATT 8 cut(s) 53, 80, 162, 246, 464, 528, 605, 840
SsiI CCGC 1 cut(s) 390
SspI AATATT 1 cut(s) 400
SspMI CTAG 1 cut(s) 30
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 743
TaqI TCGA 3 cut(s) 346, 544, 807
TasI AATT 8 cut(s) 53, 80, 162, 246, 464, 528, 605, 840
TatI WGTACW 4 cut(s) 258, 732, 738, 774
TfiI GAWTC 2 cut(s) 343, 764
Tru1I TTAA 5 cut(s) 51, 207, 581, 720, 891
Tru9I TTAA 5 cut(s) 51, 207, 581, 720, 891
TscAI CASTG 1 cut(s) 918
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 2 cut(s) 173, 714
TspRI CASTG 1 cut(s) 918
VpaK11BI GGWCC 1 cut(s) 6
XagI CCTNNNNNAGG 1 cut(s) 421
XapI RAATTY 1 cut(s) 464
XceI RCATGY 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 2 cut(s) 180, 511
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.