pycom10g03700

Endoplasmic reticulum-Golgi intermediate compartment protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0020163
Physical Location & Seq
Reverse (-)
4012252 .. 4021069
8818 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
ATGTCCCGAATCGCAGCATCCTTCCTTCCTTCCTTCCTTCCTTCCTTGTTGTGGACTGGTGAGATAGAGAGGGCAGGCACGCAGGTACGCAGACAGAGGCGAATGGGTAGGGACGGCGGTATCCTTGAGAAGCTGAGGAGTTTGGACGCCTACCCTAAAATCAATGACGATTTCTTCAGCCGTACGCTCTCTGGTGGCATCATTACCTTCGCATCTTCCCTCCTCATGTTCTTCCTCTTCGTCTCCGAGCTCGGACTATACCTCCACACTGTTACAGAGTCGAAGCTTCTTGTAGATGTTTCAAGAGGAGAAACCCTACATATCAATTTTGATGTAACTTTTCCAGCTATCCGATGCTCATTACTCAGTCTTGACACAATGGATATTAGTGGAGAGCGGCATTTTGACATACGACACGATATAGTCAAGAAACGAATTGATGCGCATGGTAATGTAATAGAAGCTAAGAAGGATGGAATTGGTGCACCAAAGATTGAGAGCCCCTTGCAAAGGCATGGTGGAAGGCTTGAGCATAATGAAAAATATTGTGGATCTTGCTTTGGAGCAGAAACGTCTGATGATGAATGCTGCAACTCTTGTGATGAAGTTCAAGAGGCATATAGGAAAAAAGGCTGGGCACTGACAAATGCAGATTTGATAGACCAGTGTGAACGGGAAGGTTTTATTCAAAAGATAAAAGATGAAGATGGTGAAGGATGTAATATTGAAGGATCCCTTGCAGTTAACAAAGTCGCGGGAAATTTTCATTTTATGCCTGGAAAAAGTTTTCACCAGTCTAACTTTCATGTCCATGATTTGCTGGCTTTCCAGATGGATAGTTACAATATAAGTCACAAGATAAACAGATTAGCTTTTGGTGACCACTTTCCTGGCATGCAAAATCCCCTTGATGGTGTACAATGGATGCAGGAAAGACCAGGTGGTATGTACCAGTATTTCATCAAGGTGGTCCCTACGCTATACACCAATATTAGAGGCCGTACAATTCGCTCAAATCAGTACTCTGTGACAGAGCATTATAAGAGTTCAGAGCTGGGACACTCTCAATTGCCACCGGGAGTTTTCTTCTATTATGATCTTTCTCCAATTAAGGTCACATTCAAAGAAGAACATGTTCCGTTTTTACACTTCGTGACCCATATTTGTGCCATAGTTGGAGGTATATTTACCGTTGCAGGACTTGTAGATTCGTTTATATATTACGGTCAAAGAGCAATTAGGAAGATGGAAGCTGGAAAATTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPIIcoated_ERV ERGIC_N

Protein Analysis

423

Amino Acids

47.7

Weight (kDa)

6.53

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 45 - 134 1.6e-29 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
COPIIcoated_ERV PF07970 186 - 403 1.3e-84 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22200 AT1G22200
malus_domestica MD05G1045400.v1.1 MD10G1052300.v1.1
prunus_persica Prupe.8G062600_v2.0.a1
pyrus_communis pycom10g03700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
Acc16I TGCGCA 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 397
AccII CGCG 1 cut(s) 755
AciI CCGC 3 cut(s) 117, 397, 755
AclWI GGATC 3 cut(s) 559, 726, 739
AcsI RAATTY 2 cut(s) 760, 1259
AcuI CTGAAG 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 1153
AfaI GTAC 6 cut(s) 87, 184, 918, 950, 1003, 1022
AfiI CCNNNNNNNGG 3 cut(s) 51, 510, 911
AflIII ACRYGT 1 cut(s) 1132
AgsI TTSAA 5 cut(s) 303, 611, 689, 728, 1123
AjnI CCWGG 3 cut(s) 775, 889, 937
AluBI AGCT 8 cut(s) 133, 250, 286, 347, 464, 872, 1054, 1253
AluI AGCT 8 cut(s) 133, 250, 286, 347, 464, 872, 1054, 1253
Alw21I GWGCWC 2 cut(s) 252, 487
Alw26I GTCTC 1 cut(s) 247
Alw44I GTGCAC 1 cut(s) 483
AlwI GGATC 3 cut(s) 559, 726, 739
AoxI GGCC 1 cut(s) 997
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 2 cut(s) 14, 588
ApoI RAATTY 2 cut(s) 760, 1259
Asp700I GAANNNNTTC 1 cut(s) 1134
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 970
AsuC2I CCSGG 1 cut(s) 1077
AsuHPI GGTGA 4 cut(s) 71, 722, 782, 890
AvaII GGWCC 1 cut(s) 970
BaeGI GKGCMC 2 cut(s) 487, 640
BaeI ACNNNNGTAYC 2 cut(s) 103, 136
BamHI GGATCC 1 cut(s) 731
BanII GRGCYC 2 cut(s) 252, 503
Bbv12I GWGCWC 2 cut(s) 252, 487
BbvCI CCTCAGC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 26, 575
BccI CCATC 5 cut(s) 467, 701, 826, 905, 1240
BceAI ACGGC 3 cut(s) 130, 165, 984
BciT130I CCWGG 3 cut(s) 777, 891, 939
BciVI GTATCC 1 cut(s) 131
BcnI CCSGG 1 cut(s) 1077
BcoDI GTCTC 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 3 cut(s) 15, 398, 589
BlsI GCNGC 3 cut(s) 16, 399, 590
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 4 cut(s) 777, 891, 939, 1077
Bme18I GGWCC 1 cut(s) 970
BmgT120I GGNCC 1 cut(s) 970
BmiI GGNNCC 2 cut(s) 733, 972
BmrFI CCNGG 4 cut(s) 777, 891, 939, 1077
BmsI GCATC 6 cut(s) 26, 207, 221, 344, 430, 915
Bpu10I CCTNAGC 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 146, 548
BpuMI CCSGG 1 cut(s) 1077
BsaHI GRCGYC 1 cut(s) 147
BsaXI ACNNNNNCTCC 2 cut(s) 246, 276
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 510, 911
Bse1I ACTGG 4 cut(s) 61, 664, 793, 952
BseBI CCWGG 3 cut(s) 777, 891, 939
BseGI GGATG 4 cut(s) 17, 478, 722, 930
BseLI CCNNNNNNNGG 3 cut(s) 51, 510, 911
BseMII CTCAG 2 cut(s) 125, 379
BseNI ACTGG 4 cut(s) 61, 664, 793, 952
BseRI GAGGAG 3 cut(s) 151, 212, 321
BseSI GKGCMC 2 cut(s) 487, 640
BseXI GCAGC 2 cut(s) 26, 575
BseYI CCCAGC 2 cut(s) 633, 1054
Bsh1236I CGCG 1 cut(s) 755
BshFI GGCC 1 cut(s) 999
BsiHKAI GWGCWC 2 cut(s) 252, 487
BsiSI CCGG 1 cut(s) 1076
BsiWI CGTACG 1 cut(s) 182
BslFI GGGAC 3 cut(s) 125, 956, 1071
BslI CCNNNNNNNGG 3 cut(s) 51, 510, 911
BsmAI GTCTC 1 cut(s) 247
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 3 cut(s) 125, 956, 1071
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 1 cut(s) 999
Bsp1286I GDGCHC 4 cut(s) 252, 487, 503, 640
Bsp1407I TGTACA 1 cut(s) 916
Bsp143I GATC 3 cut(s) 551, 731, 1096
BspACI CCGC 3 cut(s) 117, 397, 755
BspANI GGCC 1 cut(s) 999
BspCNI CTCAG 2 cut(s) 126, 378
BspFNI CGCG 1 cut(s) 755
BspLI GGNNCC 2 cut(s) 733, 972
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 3 cut(s) 559, 726, 739
BsrBI CCGCTC 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 916
BsrI ACTGG 4 cut(s) 61, 664, 793, 952
BssMI GATC 3 cut(s) 551, 731, 1096
BssNI GRCGYC 1 cut(s) 147
Bst2UI CCWGG 3 cut(s) 777, 891, 939
Bst4CI ACNGT 3 cut(s) 271, 1192, 1226
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 916
BstC8I GCNNGC 4 cut(s) 76, 80, 822, 896
BstDEI CTNAG 3 cut(s) 134, 365, 465
BstEII GGTNACC 1 cut(s) 878
BstENI CCTNNNNNAGG 1 cut(s) 508
BstF5I GGATG 4 cut(s) 17, 478, 722, 930
BstFNI CGCG 1 cut(s) 755
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 554, 734, 1099
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 3 cut(s) 551, 731, 1096
BstNI CCWGG 3 cut(s) 777, 891, 939
BstNSI RCATGY 2 cut(s) 898, 1136
BstPI GGTNACC 1 cut(s) 878
BstSCI CCNGG 4 cut(s) 775, 889, 937, 1075
BstSLI GKGCMC 2 cut(s) 487, 640
BstUI CGCG 1 cut(s) 755
BstV1I GCAGC 2 cut(s) 26, 575
BstX2I RGATCY 2 cut(s) 551, 731
BstXI CCANNNNNNTGG 1 cut(s) 890
BstYI RGATCY 2 cut(s) 551, 731
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 999
BtsCI GGATG 4 cut(s) 17, 478, 722, 930
BtsIMutI CAGTG 3 cut(s) 267, 638, 671
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 4 cut(s) 76, 80, 822, 896
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 970
CseI GACGC 1 cut(s) 155
CsiI ACCWGGT 1 cut(s) 937
Csp6I GTAC 6 cut(s) 86, 183, 917, 949, 1002, 1021
CviAII CATG 7 cut(s) 226, 446, 515, 806, 812, 895, 1133
CviQI GTAC 6 cut(s) 86, 183, 917, 949, 1002, 1021
DdeI CTNAG 3 cut(s) 134, 365, 465
DpnI GATC 3 cut(s) 553, 733, 1098
DpnII GATC 3 cut(s) 551, 731, 1096
DraIII CACNNNGTG 1 cut(s) 1153
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 2 cut(s) 252, 503
Eco47I GGWCC 1 cut(s) 970
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 160
Eco91I GGTNACC 1 cut(s) 878
EcoICRI GAGCTC 1 cut(s) 250
EcoNI CCTNNNNNAGG 1 cut(s) 508
EcoO65I GGTNACC 1 cut(s) 878
EcoRII CCWGG 3 cut(s) 775, 889, 937
EcoT38I GRGCYC 2 cut(s) 252, 503
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 7 cut(s) 229, 449, 518, 809, 815, 898, 1136
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 3 cut(s) 125, 956, 1071
FatI CATG 7 cut(s) 225, 445, 514, 805, 811, 894, 1132
FauI CCCGC 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 15, 398, 589
FokI GGATG 4 cut(s) 4, 485, 729, 937
FriOI GRGCYC 2 cut(s) 252, 503
Fsp4HI GCNGC 3 cut(s) 15, 398, 589
FspAI RTGCGCAY 1 cut(s) 444
FspI TGCGCA 1 cut(s) 444
GlaI GCGC 1 cut(s) 444
GluI GCNGC 3 cut(s) 15, 398, 589
GsaI CCCAGC 2 cut(s) 637, 1058
HaeIII GGCC 1 cut(s) 999
HapII CCGG 1 cut(s) 1076
HgaI GACGC 1 cut(s) 155
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 7 cut(s) 229, 449, 518, 809, 815, 898, 1136
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 9, 278, 1208
HpaI GTTAAC 1 cut(s) 745
HpaII CCGG 1 cut(s) 1076
HphI GGTGA 4 cut(s) 71, 722, 782, 890
Hpy166II GTNNAC 5 cut(s) 54, 485, 671, 745, 917
Hpy188I TCNGA 5 cut(s) 247, 254, 353, 577, 1051
Hpy188III TCNNGA 7 cut(s) 6, 303, 371, 427, 611, 829, 1153
Hpy8I GTNNAC 5 cut(s) 54, 485, 671, 745, 917
HpyCH4III ACNGT 3 cut(s) 271, 1192, 1226
HpyCH4IV ACGT 1 cut(s) 572
HpyCH4V TGCA 8 cut(s) 485, 508, 591, 650, 740, 898, 928, 1196
HpyF3I CTNAG 3 cut(s) 134, 365, 465
HpySE526I ACGT 1 cut(s) 572
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 7 cut(s) 229, 449, 518, 809, 815, 898, 1136
HspAI GCGC 1 cut(s) 443
KspAI GTTAAC 1 cut(s) 745
Kzo9I GATC 3 cut(s) 551, 731, 1096
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 2 cut(s) 26, 575
LweI GCATC 6 cut(s) 26, 207, 221, 344, 430, 915
MabI ACCWGGT 1 cut(s) 937
MaeII ACGT 1 cut(s) 572
MaeIII GTNAC 8 cut(s) 271, 334, 839, 851, 878, 1027, 1114, 1153
MalI GATC 3 cut(s) 553, 733, 1098
MbiI CCGCTC 1 cut(s) 397
MboI GATC 3 cut(s) 551, 731, 1096
MboII GAAGA 8 cut(s) 166, 207, 223, 229, 716, 1078, 1139, 1255
MfeI CAATTG 1 cut(s) 1067
MflI RGATCY 2 cut(s) 551, 731
MhlI GDGCHC 4 cut(s) 252, 487, 503, 640
MluCI AATT 9 cut(s) 325, 435, 477, 760, 1005, 1067, 1107, 1236, 1259
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 1156
MroXI GAANNNNTTC 1 cut(s) 1134
MseI TTAA 2 cut(s) 744, 1110
MslI CAYNNNNRTG 4 cut(s) 450, 810, 965, 1164
MspI CCGG 1 cut(s) 1076
MspR9I CCNGG 4 cut(s) 777, 891, 939, 1077
MunI CAATTG 1 cut(s) 1067
Mva1269I GAATGC 1 cut(s) 590
MvaI CCWGG 3 cut(s) 777, 891, 939
MvnI CGCG 1 cut(s) 755
NciI CCSGG 1 cut(s) 1077
NdeII GATC 3 cut(s) 551, 731, 1096
NlaIII CATG 7 cut(s) 229, 449, 518, 809, 815, 898, 1136
NlaIV GGNNCC 2 cut(s) 733, 972
NmuCI GTSAC 5 cut(s) 851, 878, 1027, 1114, 1153
NsbI TGCGCA 1 cut(s) 444
NspI RCATGY 2 cut(s) 898, 1136
PaeI GCATGC 1 cut(s) 898
PciI ACATGT 1 cut(s) 1132
PctI GAATGC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 1134
PfeI GAWTC 2 cut(s) 9, 1208
Pfl23II CGTACG 1 cut(s) 182
PkrI GCNGC 3 cut(s) 16, 399, 590
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PscI ACATGT 1 cut(s) 1132
PsiI TTATAA 1 cut(s) 1041
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 3 cut(s) 775, 889, 937
PspEI GGTNACC 1 cut(s) 878
PspFI CCCAGC 2 cut(s) 633, 1054
PspGI CCWGG 3 cut(s) 775, 889, 937
PspLI CGTACG 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 733, 972
PspPI GGNCC 1 cut(s) 970
PsuI RGATCY 2 cut(s) 551, 731
RsaI GTAC 6 cut(s) 87, 184, 918, 950, 1003, 1022
RsaNI GTAC 6 cut(s) 86, 183, 917, 949, 1002, 1021
RseI CAYNNNNRTG 4 cut(s) 450, 810, 965, 1164
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 744, 1110
SatI GCNGC 3 cut(s) 15, 398, 589
Sau3AI GATC 3 cut(s) 551, 731, 1096
Sau96I GGNCC 1 cut(s) 970
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 4 cut(s) 777, 891, 939, 1077
SduI GDGCHC 4 cut(s) 252, 487, 503, 640
SexAI ACCWGGT 1 cut(s) 937
SfaNI GCATC 6 cut(s) 26, 207, 221, 344, 430, 915
SinI GGWCC 1 cut(s) 970
SmiMI CAYNNNNRTG 4 cut(s) 450, 810, 965, 1164
SmlI CTYRAG 2 cut(s) 125, 527
SmoI CTYRAG 2 cut(s) 125, 527
SphI GCATGC 1 cut(s) 898
Sse9I AATT 9 cut(s) 325, 435, 477, 760, 1005, 1067, 1107, 1236, 1259
SsiI CCGC 3 cut(s) 117, 397, 755
SspI AATATT 3 cut(s) 545, 724, 991
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 4 cut(s) 775, 889, 937, 1075
TaaI ACNGT 3 cut(s) 271, 1192, 1226
TaiI ACGT 1 cut(s) 575
TaqI TCGA 1 cut(s) 281
TasI AATT 9 cut(s) 325, 435, 477, 760, 1005, 1067, 1107, 1236, 1259
TatI WGTACW 2 cut(s) 916, 1020
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 2 cut(s) 9, 1208
Tru1I TTAA 2 cut(s) 744, 1110
Tru9I TTAA 2 cut(s) 744, 1110
TscAI CASTG 3 cut(s) 274, 645, 671
TseFI GTSAC 5 cut(s) 851, 878, 1027, 1114, 1153
TseI GCWGC 2 cut(s) 14, 588
Tsp45I GTSAC 5 cut(s) 851, 878, 1027, 1114, 1153
TspDTI ATGAA 7 cut(s) 552, 597, 618, 717, 755, 794, 949
TspGWI ACGGA 1 cut(s) 1128
TspRI CASTG 3 cut(s) 274, 645, 671
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 970
XagI CCTNNNNNAGG 1 cut(s) 508
XapI RAATTY 2 cut(s) 760, 1259
XceI RCATGY 2 cut(s) 898, 1136
XmnI GAANNNNTTC 1 cut(s) 1134
ZrmI AGTACT 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.