pycom10g13920

GPI transamidase component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009321
Physical Location & Seq
Forward (+)
17381724 .. 17384023
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1407 bp
ATGTCACGCACCGCACTTCTCTTAAGAAAAAAAGGCGAAACGAAGAGGGTTAAAATCACTAGGCAAATAAAAAGAGAGGAACCTCAAAACGAGGGGTGTGGTAAAACTTGGAACCACTGGCAGAACAACTCGGAAAATAAAAAAAATGAAAAAGAAACGCCGTCTGTGGGAATCGAACCCACGACCACACGGTTAAAAGCCGTTAAACCAACAGCTCCAACTCTAAGTCAAGAGCTTCAGAGCTCCGGTGCCTCCACAACAATGGCGGAAATCACAGAACCTCTCCTCCCTCCCAACGAAATCCCACAAACATCCAAACCGCAAGAGCCAGCCGATGACTTCGATCCCAAAACGATGCGCAAAACCAAGCCAGGACTCAAGCGCCTGATCATCACCGTCTCGGTCCTCTTCTCCTTCATCCTAGGAATCCCATTGATATACAAATCCGTGGAAATCTACCGAGCGCCGTTGCCGTTTCGGGAAATCGATTCCCTCTCAGCTGAAATTGATTCCAATCCGCTTCTGTTCCCATGTCGTTTCCAAGCAATCTTCATTGGCTTCGACTGGGAATCCTCCATTACCACTCTGGAATCTTCCATCCTAACCAAAATGACCAAATTTACCTCCCAGACCCCTCAATGCGGCACCTGTAGCCCCAACCACACCGTCTCGGCAGTCCTAGACTCCGATTCCAGTTGTCTGCAATCCCAAACCGGTCCGTGTTCGACGAAATGCGGCGCCTTTAAGGCCATTGATTTCGACGGCGCCGATGAGGCGGTGGATGAGGCTCTGGAGTCTGTGTTTGGAGGTTGTTCGGGTTCCGGTTCAGGTGGAGAGGTGTATAGTGTGGTGGTGGTGAATAGGGAACATGAGGTGAGAGCAGTGGTGGGGAAGTACAGGCACGCTTGGATTGCCGGCAGGGTTTCGGAGGCGGAGGCGGTGTCGAGGGCAGCGGAGATGTTTGTCAAAGTGTTTGTGAATGGCGGGAAGGAGGAAGGTTTGATTCAGGGGGAGTTTATGCCTGTTGGGGCTGATGGAATGGTTGTTCTTTCGTTTAATTTGCTGAATGCAGACCCTCGTGATTGGGTTTACGATTGGGACTTTCAAACTGTAGACAAGACGTTGTTGGCCCCTATTATCGAGGCTATGAAACCTGTAGCAAACATAAGCGTGGAAAGTCAGGTGTTATATCATACGCCGAAGTCCTCATTGTCTTACTGGGATGACAAGCGGGAGAGCTACATCTTTAGTACCAAGGATCTACCTTTCTTTGTGAATTCAAATGAGTGGCACTTGGATACATCTATTGCAGCTGGTGGGAGGTCAAAGATGTTGCACTTTGTGATATATACCGTCCGAAAAGGAGTGCCCTCTCCTCCTGCAGCTTCCAGATGGAGAGATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-S

Protein Analysis

469

Amino Acids

51.82

Weight (kDa)

6.08

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-S PF10510 134 - 452 1.7e-46 Phosphatidylinositol-glycan biosynthesis class S protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 359
AccB1I GGYRCC 4 cut(s) 248, 644, 737, 764
AccI GTMKAC 1 cut(s) 1113
AclWI GGATC 2 cut(s) 338, 1266
AcsI RAATTY 2 cut(s) 617, 1276
AcuI CTGAAG 1 cut(s) 221
AcyI GRCGYC 2 cut(s) 738, 765
AdeI CACNNNGTG 1 cut(s) 1342
AfaI GTAC 2 cut(s) 896, 1252
AfiI CCNNNNNNNGG 2 cut(s) 167, 641
AflII CTTAAG 1 cut(s) 22
AgeI ACCGGT 1 cut(s) 713
AgsI TTSAA 2 cut(s) 1106, 1281
AjnI CCWGG 1 cut(s) 370
AloI GAACNNNNNNTCC 4 cut(s) 270, 302, 1029, 1061
AluBI AGCT 7 cut(s) 215, 235, 243, 500, 1239, 1313, 1385
AluI AGCT 7 cut(s) 215, 235, 243, 500, 1239, 1313, 1385
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 2 cut(s) 403, 673
AlwI GGATC 2 cut(s) 338, 1266
AoxI GGCC 2 cut(s) 747, 1128
ApeKI GCWGC 3 cut(s) 950, 1310, 1382
ApoI RAATTY 2 cut(s) 617, 1276
AsiGI ACCGGT 1 cut(s) 713
AspA2I CCTAGG 1 cut(s) 421
AspLEI GCGC 5 cut(s) 360, 384, 466, 740, 767
AspS9I GGNCC 3 cut(s) 403, 716, 1129
AsuHPI GGTGA 3 cut(s) 385, 868, 886
AvaII GGWCC 2 cut(s) 403, 716
AvrII CCTAGG 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 1371
BanI GGYRCC 4 cut(s) 248, 644, 737, 764
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 1077
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 3 cut(s) 962, 1322, 1394
BccI CCATC 3 cut(s) 605, 1028, 1386
BceAI ACGGC 5 cut(s) 145, 185, 451, 457, 778
BciT130I CCWGG 1 cut(s) 372
BciVI GTATCC 1 cut(s) 1291
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 2 cut(s) 403, 673
BfaI CTAG 3 cut(s) 60, 422, 680
BfmI CTRYAG 4 cut(s) 649, 1110, 1155, 1380
BfoI RGCGCY 4 cut(s) 385, 467, 741, 768
BfrI CTTAAG 1 cut(s) 22
BfuI GTATCC 1 cut(s) 1291
BglI GCCNNNNNGGC 2 cut(s) 746, 773
BisI GCNGC 5 cut(s) 643, 736, 951, 1311, 1383
BlnI CCTAGG 1 cut(s) 421
BlsI GCNGC 5 cut(s) 644, 737, 952, 1312, 1384
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 403, 716
BmgT120I GGNCC 3 cut(s) 403, 716, 1129
BmiI GGNNCC 8 cut(s) 81, 113, 250, 646, 739, 766, 820, 1131
BmrFI CCNGG 1 cut(s) 372
BmrI ACTGGG 2 cut(s) 574, 1228
BmsI GCATC 1 cut(s) 345
BmuI ACTGGG 2 cut(s) 574, 1228
BpmI CTGGAG 1 cut(s) 812
BpuEI CTTGAG 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 513
BsaHI GRCGYC 2 cut(s) 738, 765
BsaJI CCNNGG 3 cut(s) 421, 447, 1254
BsaWI WCCGGW 3 cut(s) 245, 713, 821
BsaXI ACNNNNNCTCC 6 cut(s) 270, 300, 785, 815, 926, 956
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 641
Bse118I RCCGGY 2 cut(s) 713, 914
Bse1I ACTGG 4 cut(s) 122, 569, 693, 1223
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 1 cut(s) 372
BseCI ATCGAT 1 cut(s) 486
BseDI CCNNGG 3 cut(s) 421, 447, 1254
BseGI GGATG 5 cut(s) 311, 417, 597, 787, 1228
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 2 cut(s) 167, 641
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 4 cut(s) 122, 569, 693, 1223
BseRI GAGGAG 2 cut(s) 275, 1365
BseSI GKGCMC 1 cut(s) 1371
BseXI GCAGC 3 cut(s) 962, 1322, 1394
BshFI GGCC 2 cut(s) 749, 1130
BshNI GGYRCC 4 cut(s) 248, 644, 737, 764
BshTI ACCGGT 1 cut(s) 713
BshVI ATCGAT 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 4 cut(s) 246, 714, 822, 915
BslFI GGGAC 1 cut(s) 1112
BslI CCNNNNNNNGG 2 cut(s) 167, 641
BsmAI GTCTC 2 cut(s) 403, 673
BsmBI CGTCTC 2 cut(s) 403, 673
BsmFI GGGAC 1 cut(s) 1112
BsmI GAATGC 1 cut(s) 1072
BsnI GGCC 2 cut(s) 749, 1130
Bsp1286I GDGCHC 2 cut(s) 245, 1371
Bsp143I GATC 3 cut(s) 343, 387, 1258
BspANI GGCC 2 cut(s) 749, 1130
BspCNI CTCAG 1 cut(s) 509
BspDI ATCGAT 1 cut(s) 486
BspLI GGNNCC 8 cut(s) 81, 113, 250, 646, 739, 766, 820, 1131
BspMAI CTGCAG 1 cut(s) 1384
BspPI GGATC 2 cut(s) 338, 1266
BspT107I GGYRCC 4 cut(s) 248, 644, 737, 764
BspTI CTTAAG 1 cut(s) 22
BsrFI RCCGGY 2 cut(s) 713, 914
BsrI ACTGG 4 cut(s) 122, 569, 693, 1223
BssAI RCCGGY 2 cut(s) 713, 914
BssECI CCNNGG 3 cut(s) 421, 447, 1254
BssMI GATC 3 cut(s) 343, 387, 1258
BssNI GRCGYC 2 cut(s) 738, 765
BssSI CACGAG 1 cut(s) 1077
BssT1I CCWWGG 2 cut(s) 421, 1254
Bst2BI CACGAG 1 cut(s) 1077
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 5 cut(s) 192, 397, 667, 1111, 1354
Bst6I CTCTTC 2 cut(s) 38, 413
BstACI GRCGYC 2 cut(s) 738, 765
BstAFI CTTAAG 1 cut(s) 22
BstC8I GCNNGC 3 cut(s) 330, 903, 916
BstDEI CTNAG 2 cut(s) 224, 496
BstDSI CCRYGG 1 cut(s) 447
BstF5I GGATG 5 cut(s) 311, 417, 597, 787, 1228
BstH2I RGCGCY 4 cut(s) 385, 467, 741, 768
BstHHI GCGC 5 cut(s) 360, 384, 466, 740, 767
BstKTI GATC 3 cut(s) 346, 390, 1261
BstMAI GTCTC 2 cut(s) 403, 673
BstMBI GATC 3 cut(s) 343, 387, 1258
BstMWI GCNNNNNNNGC 4 cut(s) 651, 746, 773, 911
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 4 cut(s) 649, 1110, 1155, 1380
BstSLI GKGCMC 1 cut(s) 1371
BstV1I GCAGC 3 cut(s) 962, 1322, 1394
BstX2I RGATCY 1 cut(s) 1258
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 1258
Bsu15I ATCGAT 1 cut(s) 486
BsuI GTATCC 1 cut(s) 1291
BsuRI GGCC 2 cut(s) 749, 1130
BsuTUI ATCGAT 1 cut(s) 486
BtgI CCRYGG 1 cut(s) 447
BtsCI GGATG 5 cut(s) 311, 417, 597, 787, 1228
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 2 cut(s) 115, 888
Cac8I GCNNGC 3 cut(s) 330, 903, 916
CfoI GCGC 5 cut(s) 360, 384, 466, 740, 767
Cfr10I RCCGGY 2 cut(s) 713, 914
Cfr13I GGNCC 3 cut(s) 403, 716, 1129
ClaI ATCGAT 1 cut(s) 486
CpoI CGGWCCG 1 cut(s) 716
Csp6I GTAC 2 cut(s) 895, 1251
CspAI ACCGGT 1 cut(s) 713
CspI CGGWCCG 1 cut(s) 716
CviAII CATG 2 cut(s) 531, 869
CviQI GTAC 2 cut(s) 895, 1251
DdeI CTNAG 2 cut(s) 224, 496
DinI GGCGCC 2 cut(s) 739, 766
DpnI GATC 3 cut(s) 345, 389, 1260
DpnII GATC 3 cut(s) 343, 387, 1258
DraIII CACNNNGTG 1 cut(s) 1342
Eam1104I CTCTTC 2 cut(s) 38, 413
EarI CTCTTC 2 cut(s) 38, 413
EciI GGCGGA 2 cut(s) 281, 947
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 421, 1254
Eco24I GRGCYC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 403, 716
Eco53kI GAGCTC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 2 cut(s) 421, 1254
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 2 cut(s) 739, 766
EheI GGCGCC 2 cut(s) 739, 766
ErhI CCWWGG 2 cut(s) 421, 1254
Esp3I CGTCTC 2 cut(s) 403, 673
FaeI CATG 2 cut(s) 534, 872
FaqI GGGAC 1 cut(s) 1112
FatI CATG 2 cut(s) 530, 868
FauI CCCGC 2 cut(s) 977, 1224
FbaI TGATCA 1 cut(s) 387
FblI GTMKAC 1 cut(s) 1113
Fnu4HI GCNGC 5 cut(s) 643, 736, 951, 1311, 1383
FokI GGATG 5 cut(s) 298, 404, 584, 794, 1235
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 5 cut(s) 643, 736, 951, 1311, 1383
FspBI CTAG 3 cut(s) 60, 422, 680
FspI TGCGCA 1 cut(s) 359
GlaI GCGC 5 cut(s) 359, 383, 465, 739, 766
GluI GCNGC 5 cut(s) 643, 736, 951, 1311, 1383
GsuI CTGGAG 1 cut(s) 812
HaeII RGCGCY 4 cut(s) 385, 467, 741, 768
HaeIII GGCC 2 cut(s) 749, 1130
HapII CCGG 4 cut(s) 246, 714, 822, 915
HhaI GCGC 5 cut(s) 360, 384, 466, 740, 767
Hin1I GRCGYC 2 cut(s) 738, 765
Hin1II CATG 2 cut(s) 534, 872
Hin6I GCGC 5 cut(s) 358, 382, 464, 738, 765
HinP1I GCGC 5 cut(s) 358, 382, 464, 738, 765
HpaII CCGG 4 cut(s) 246, 714, 822, 915
HphI GGTGA 3 cut(s) 385, 868, 886
Hpy166II GTNNAC 2 cut(s) 1090, 1114
Hpy188I TCNGA 5 cut(s) 133, 240, 688, 928, 1358
Hpy188III TCNNGA 6 cut(s) 230, 479, 587, 791, 1079, 1389
Hpy8I GTNNAC 2 cut(s) 1090, 1114
Hpy99I CGWCG 2 cut(s) 730, 764
HpyAV CCTTC 3 cut(s) 424, 982, 989
HpyCH4III ACNGT 5 cut(s) 192, 397, 667, 1111, 1354
HpyCH4IV ACGT 1 cut(s) 1121
HpyCH4V TGCA 5 cut(s) 703, 1070, 1310, 1336, 1382
HpyF10VI GCNNNNNNNGC 4 cut(s) 651, 746, 773, 911
HpyF3I CTNAG 2 cut(s) 224, 496
HpySE526I ACGT 1 cut(s) 1121
Hsp92I GRCGYC 2 cut(s) 738, 765
Hsp92II CATG 2 cut(s) 534, 872
HspAI GCGC 5 cut(s) 358, 382, 464, 738, 765
KasI GGCGCC 2 cut(s) 737, 764
KroI GCCGGC 1 cut(s) 914
KroNI GCCGGC 1 cut(s) 916
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 3 cut(s) 343, 387, 1258
LmnI GCTCC 2 cut(s) 220, 248
Lsp1109I GCAGC 3 cut(s) 962, 1322, 1394
LweI GCATC 1 cut(s) 345
MaeI CTAG 3 cut(s) 60, 422, 680
MaeII ACGT 1 cut(s) 1121
MaeIII GTNAC 1 cut(s) 3
MalI GATC 3 cut(s) 345, 389, 1260
MboI GATC 3 cut(s) 343, 387, 1258
MboII GAAGA 4 cut(s) 55, 400, 541, 585
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 2 cut(s) 245, 1371
MluCI AATT 4 cut(s) 504, 617, 1057, 1276
Mly113I GGCGCC 2 cut(s) 738, 765
MlyI GAGTC 3 cut(s) 369, 677, 803
MmeI TCCRAC 1 cut(s) 242
MroNI GCCGGC 1 cut(s) 914
MseI TTAA 6 cut(s) 23, 51, 194, 204, 744, 1056
MslI CAYNNNNRTG 2 cut(s) 260, 1169
MspA1I CMGCKG 3 cut(s) 500, 953, 1313
MspCI CTTAAG 1 cut(s) 22
MspI CCGG 4 cut(s) 246, 714, 822, 915
MspR9I CCNGG 1 cut(s) 372
Mva1269I GAATGC 1 cut(s) 1072
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 651, 746, 773, 911
NaeI GCCGGC 1 cut(s) 916
NarI GGCGCC 2 cut(s) 738, 765
NdeII GATC 3 cut(s) 343, 387, 1258
NgoMIV GCCGGC 1 cut(s) 914
NlaIII CATG 2 cut(s) 534, 872
NlaIV GGNNCC 8 cut(s) 81, 113, 250, 646, 739, 766, 820, 1131
NmeAIII GCCGAG 1 cut(s) 650
NmuCI GTSAC 1 cut(s) 3
NsbI TGCGCA 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PctI GAATGC 1 cut(s) 1072
PdiI GCCGGC 1 cut(s) 916
PfeI GAWTC 8 cut(s) 171, 426, 488, 509, 569, 590, 689, 1003
PinAI ACCGGT 1 cut(s) 713
PkrI GCNGC 5 cut(s) 644, 737, 952, 1312, 1384
PleI GAGTC 3 cut(s) 369, 677, 802
PluTI GGCGCC 2 cut(s) 741, 768
PpsI GAGTC 3 cut(s) 369, 677, 802
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 8 cut(s) 81, 113, 250, 646, 739, 766, 820, 1131
PspPI GGNCC 3 cut(s) 403, 716, 1129
PstI CTGCAG 1 cut(s) 1384
PsuI RGATCY 1 cut(s) 1258
PvuII CAGCTG 2 cut(s) 500, 1313
RsaI GTAC 2 cut(s) 896, 1252
RsaNI GTAC 2 cut(s) 895, 1251
RseI CAYNNNNRTG 2 cut(s) 260, 1169
Rsr2I CGGWCCG 1 cut(s) 716
RsrII CGGWCCG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 6 cut(s) 23, 51, 194, 204, 744, 1056
SatI GCNGC 5 cut(s) 643, 736, 951, 1311, 1383
Sau3AI GATC 3 cut(s) 343, 387, 1258
Sau96I GGNCC 3 cut(s) 403, 716, 1129
SchI GAGTC 3 cut(s) 369, 677, 803
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 2 cut(s) 245, 1371
SfaNI GCATC 1 cut(s) 345
SfcI CTRYAG 4 cut(s) 649, 1110, 1155, 1380
SfoI GGCGCC 2 cut(s) 739, 766
SinI GGWCC 2 cut(s) 403, 716
SmiMI CAYNNNNRTG 2 cut(s) 260, 1169
SmlI CTYRAG 2 cut(s) 22, 377
SmoI CTYRAG 2 cut(s) 22, 377
Sse9I AATT 4 cut(s) 504, 617, 1057, 1276
SspDI GGCGCC 2 cut(s) 737, 764
SspMI CTAG 3 cut(s) 60, 422, 680
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 2 cut(s) 421, 1254
TaaI ACNGT 5 cut(s) 192, 397, 667, 1111, 1354
TaiI ACGT 1 cut(s) 1124
TaqI TCGA 8 cut(s) 174, 342, 486, 561, 725, 759, 944, 1140
TaqII GACCGA 1 cut(s) 391
TasI AATT 4 cut(s) 504, 617, 1057, 1276
TatI WGTACW 1 cut(s) 894
TauI GCSGC 2 cut(s) 645, 738
TfiI GAWTC 8 cut(s) 171, 426, 488, 509, 569, 590, 689, 1003
Tru1I TTAA 6 cut(s) 23, 51, 194, 204, 744, 1056
Tru9I TTAA 6 cut(s) 23, 51, 194, 204, 744, 1056
TscAI CASTG 2 cut(s) 122, 888
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 3 cut(s) 950, 1310, 1382
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 4 cut(s) 162, 406, 541, 1163
TspGWI ACGGA 2 cut(s) 436, 708
TspRI CASTG 2 cut(s) 122, 888
Vha464I CTTAAG 1 cut(s) 22
VpaK11BI GGWCC 2 cut(s) 403, 716
XapI RAATTY 2 cut(s) 617, 1276
XcmI CCANNNNNNNNNTGG 1 cut(s) 583
XmaJI CCTAGG 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 1113
XspI CTAG 3 cut(s) 60, 422, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.