pycom10g17300

Peptidase, trypsin-like serine and cysteine proteases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009001
Physical Location & Seq
Forward (+)
20513264 .. 20514404
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGGAGCGGAGTAGATTTAATATGAGGATGCGTTGTTCGGGTTCAACGCTATCAGATGAGTCAGCCTTGGATCTTGAGAGAAACTGTTGCAGTCATTCTAATTTACCTTCGTTAAGTCCACCAACACTTCAACCCTTTGCATCAGCTGGACAGCATTCTGAGACTAGCGCTGCTTACTTCTCGTGGCCTACCTCAAGCCGATTGAGTGATGCTGCTGAAGAGAGGGCAAACTATTTCACTAATCTACAGAAAGGGGTCCTGCCTGAAACTCTTGGTCAGTTGCCAAAAGGGCAGCAAGCGAATACATTGCTTGAACTGATGACTATAAGGGCATTTCATAGCAAGATTTTGCGATGTTATAGTCTTGGAACAGCAATTGGGTTTCGTATTCGACGGGGTGTGTTAACTGACATACCAGCTATTCTTGTCTTTGTTTCAAGAAAAGTTCACAAGCAATGGCTCAGCCCAATACAGTGTTTACCCACTGCCCTAGAGGGACCTGGAGGTGTATGGTGTGATGTGGACGTTGTGGAATTCTCATATTTTGGTGCACCCGAGCCAGCTCCCAAAGAACAATTGTACACAGAAATTGTAGATGATTTGCGTGGAGGTTCGATCCGTGCATTGGCTCAGGTTCTCAGGTGGCAAGCCAGGAAACTTATGGAACATTGGGTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.27

Weight (kDa)

8.24

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAL1_N PF25608 98 - 177 5.4e-45 NARROW LEAF 1 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 7
AciI CCGC 1 cut(s) 7
AclWI GGATC 2 cut(s) 78, 610
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 1 cut(s) 581
AfeI AGCGCT 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 625
AgsI TTSAA 4 cut(s) 45, 131, 314, 438
AjnI CCWGG 2 cut(s) 499, 650
AloI GAACNNNNNNTCC 2 cut(s) 19, 51
AluBI AGCT 3 cut(s) 146, 419, 563
AluI AGCT 3 cut(s) 146, 419, 563
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 1 cut(s) 155
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 2 cut(s) 78, 610
Ama87I CYCGRG 1 cut(s) 554
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 1 cut(s) 185
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 3 cut(s) 170, 212, 292
ApoI RAATTY 1 cut(s) 533
AspLEI GCGC 1 cut(s) 170
AspS9I GGNCC 2 cut(s) 256, 497
AvaI CYCGRG 1 cut(s) 554
AvaII GGWCC 2 cut(s) 256, 497
BaeGI GKGCMC 1 cut(s) 553
BauI CACGAG 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 3 cut(s) 157, 199, 304
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 2 cut(s) 501, 652
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 2 cut(s) 165, 491
BfmI CTRYAG 1 cut(s) 245
BfoI RGCGCY 1 cut(s) 171
BglI GCCNNNNNGGC 1 cut(s) 289
BisI GCNGC 3 cut(s) 171, 213, 293
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 3 cut(s) 172, 214, 294
Bme1390I CCNGG 2 cut(s) 501, 652
Bme18I GGWCC 2 cut(s) 256, 497
BmeT110I CYCGRG 1 cut(s) 554
BmgT120I GGNCC 2 cut(s) 256, 497
BmiI GGNNCC 2 cut(s) 257, 498
BmrFI CCNGG 2 cut(s) 501, 652
BmsI GCATC 3 cut(s) 18, 149, 199
BpmI CTGGAG 1 cut(s) 522
Bpu10I CCTNAGC 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 461
BpuEI CTTGAG 2 cut(s) 95, 178
BsaJI CCNNGG 1 cut(s) 66
Bsc4I CCNNNNNNNGG 1 cut(s) 625
Bse3DI GCAATG 2 cut(s) 305, 461
BseBI CCWGG 2 cut(s) 501, 652
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 625
BseMI GCAATG 2 cut(s) 305, 461
BseMII CTCAG 4 cut(s) 150, 475, 644, 652
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 3 cut(s) 157, 199, 304
BshFI GGCC 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 554
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 625
BsmAI GTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 510
BsmI GAATGC 1 cut(s) 154
BsnI GGCC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 553
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 2 cut(s) 70, 615
Bsp1720I GCTNAGC 1 cut(s) 461
BspACI CCGC 1 cut(s) 7
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 4 cut(s) 151, 474, 643, 651
BspLI GGNNCC 2 cut(s) 257, 498
BspPI GGATC 2 cut(s) 78, 610
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 2 cut(s) 305, 461
BsrGI TGTACA 1 cut(s) 579
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 2 cut(s) 70, 615
BssSI CACGAG 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 66
Bst2BI CACGAG 1 cut(s) 181
Bst2UI CCWGG 2 cut(s) 501, 652
Bst4CI ACNGT 2 cut(s) 86, 474
Bst6I CTCTTC 1 cut(s) 213
BstAUI TGTACA 1 cut(s) 579
BstC8I GCNNGC 3 cut(s) 297, 561, 648
BstDEI CTNAG 4 cut(s) 159, 461, 630, 638
BstF5I GGATG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 2 cut(s) 73, 618
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 2 cut(s) 70, 615
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNI CCWGG 2 cut(s) 501, 652
BstSCI CCNGG 2 cut(s) 499, 650
BstSFI CTRYAG 1 cut(s) 245
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 3 cut(s) 157, 199, 304
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 1 cut(s) 187
BtgZI GCGATG 1 cut(s) 367
BtsCI GGATG 1 cut(s) 33
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 2 cut(s) 479, 483
Cac8I GCNNGC 3 cut(s) 297, 561, 648
CfoI GCGC 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 256, 497
Csp6I GTAC 1 cut(s) 580
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 4 cut(s) 159, 461, 630, 638
DpnI GATC 2 cut(s) 72, 617
DpnII GATC 2 cut(s) 70, 615
Eam1104I CTCTTC 1 cut(s) 213
EarI CTCTTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 66
Eco47I GGWCC 2 cut(s) 256, 497
Eco47III AGCGCT 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 554
EcoO109I RGGNCCY 2 cut(s) 256, 497
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 2 cut(s) 499, 650
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaiI YATR 8 cut(s) 23, 326, 339, 360, 413, 511, 541, 662
FaqI GGGAC 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 171, 213, 293
FokI GGATG 1 cut(s) 40
Fsp4HI GCNGC 3 cut(s) 171, 213, 293
FspBI CTAG 2 cut(s) 165, 491
GlaI GCGC 1 cut(s) 169
GluI GCNGC 3 cut(s) 171, 213, 293
GsuI CTGGAG 1 cut(s) 522
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 187
HhaI GCGC 1 cut(s) 170
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HinfI GANTC 1 cut(s) 59
HpaI GTTAAC 1 cut(s) 405
Hpy166II GTNNAC 7 cut(s) 119, 405, 448, 479, 523, 551, 582
Hpy188I TCNGA 2 cut(s) 55, 160
Hpy188III TCNNGA 2 cut(s) 74, 438
Hpy8I GTNNAC 7 cut(s) 119, 405, 448, 479, 523, 551, 582
Hpy99I CGWCG 1 cut(s) 396
HpyAV CCTTC 1 cut(s) 117
HpyCH4III ACNGT 2 cut(s) 86, 474
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 5 cut(s) 90, 140, 551, 623, 676
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 4 cut(s) 159, 461, 630, 638
HpySE526I ACGT 1 cut(s) 525
HspAI GCGC 1 cut(s) 168
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 2 cut(s) 70, 615
LmnI GCTCC 2 cut(s) 4, 568
Lsp1109I GCAGC 3 cut(s) 157, 199, 304
LweI GCATC 3 cut(s) 18, 149, 199
MaeI CTAG 2 cut(s) 165, 491
MaeII ACGT 1 cut(s) 525
MalI GATC 2 cut(s) 72, 617
MbiI CCGCTC 1 cut(s) 7
MboI GATC 2 cut(s) 70, 615
MboII GAAGA 1 cut(s) 230
MfeI CAATTG 2 cut(s) 375, 575
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 5 cut(s) 100, 375, 533, 575, 588
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 6 cut(s) 18, 202, 216, 487, 497, 602
MseI TTAA 3 cut(s) 18, 113, 404
MspA1I CMGCKG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 501, 652
MunI CAATTG 2 cut(s) 375, 575
Mva1269I GAATGC 1 cut(s) 154
MvaI CCWGG 2 cut(s) 501, 652
MwoI GCNNNNNNNGC 1 cut(s) 289
NdeII GATC 2 cut(s) 70, 615
NlaIV GGNNCC 2 cut(s) 257, 498
PctI GAATGC 1 cut(s) 154
PkrI GCNGC 3 cut(s) 172, 214, 294
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PpuMI RGGWCCY 2 cut(s) 256, 497
Psp5II RGGWCCY 2 cut(s) 256, 497
Psp6I CCWGG 2 cut(s) 499, 650
PspGI CCWGG 2 cut(s) 499, 650
PspN4I GGNNCC 2 cut(s) 257, 498
PspPI GGNCC 2 cut(s) 256, 497
PspPPI RGGWCCY 2 cut(s) 256, 497
PsuI RGATCY 1 cut(s) 70
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
SaqAI TTAA 3 cut(s) 18, 113, 404
SatI GCNGC 3 cut(s) 171, 213, 293
Sau3AI GATC 2 cut(s) 70, 615
Sau96I GGNCC 2 cut(s) 256, 497
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 2 cut(s) 501, 652
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 3 cut(s) 18, 149, 199
SfcI CTRYAG 1 cut(s) 245
SinI GGWCC 2 cut(s) 256, 497
SmlI CTYRAG 2 cut(s) 74, 193
SmoI CTYRAG 2 cut(s) 74, 193
Sse9I AATT 5 cut(s) 100, 375, 533, 575, 588
SsiI CCGC 1 cut(s) 7
SspMI CTAG 2 cut(s) 165, 491
StyD4I CCNGG 2 cut(s) 499, 650
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 86, 474
TaiI ACGT 1 cut(s) 528
TaqI TCGA 2 cut(s) 391, 614
TasI AATT 5 cut(s) 100, 375, 533, 575, 588
TatI WGTACW 1 cut(s) 579
Tru1I TTAA 3 cut(s) 18, 113, 404
Tru9I TTAA 3 cut(s) 18, 113, 404
TscAI CASTG 2 cut(s) 479, 490
TseI GCWGC 3 cut(s) 170, 212, 292
TspDTI ATGAA 1 cut(s) 326
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 2 cut(s) 479, 490
VneI GTGCAC 1 cut(s) 549
VpaK11BI GGWCC 2 cut(s) 256, 497
XapI RAATTY 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XspI CTAG 2 cut(s) 165, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.