pycom10g19360

APO protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0020141
Physical Location & Seq
Forward (+)
22469854 .. 22471825
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGTTCCAGAATCCTCCAGCAGTATCCTCTGCTTTGCGGGAGCCATATCCGAAAGGTGCTTGCCTCTGTACCGTTGAGTTCAAGTTGCCTCAGTTATCAGCTGCAAGATCGTACAGTCTTGGGTTGAAGTTTGAGCATGGAAAACTTCATAAAGGAGGAAGTATATTTGGAACTATCTTTGCTGCCAGTAGGAAGACCAGAGTAGAACCAACTTCTAGGAAGCAAGAAGCATATCCCCAGAATGTGGATCGTCCACCTGTACTACCTAAGAATAAGAAGAAACCGTATCCTATTCCCTTAAAGAAGATCAAGCAGTTTGCAAAGGAGGACAAGAAACTTGCAGAATTGGGTATAGAGAAGCCCCTTGAACCTCCAAAAAATGGATTACTTGCCACTGATCTGATTCCTGTTGCTCACCAAGTACTAGATTCCTGGAAAGTTTTAATTAAAGGCATTGCGCAGCTGTTGCACATCATTCCTGTATACGGATGCACTGAATGCTCTGAGGTTCATGTAAGCCACTCTGGTCATCACATACAGGACTGTCTTGGTTCAACCAGTGCTAAACGCCGAAGCTTCCACTCCTGGATCAAGGGTTCCATCAATGACGTACTTGTACCCATCGAGGCATATCATCTCTTTGACCCTTTTGGTCGACGCATTAAGCATGAAACTAGATTTCAATACGACAGGATTCCAGCTGTTGTGGAGCTGTGCATCCAAGCTGGTGTTGATCGATCCCTGAGTACCCTTCACGTCGAAGGACCAAACCCATCCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

261

Amino Acids

28.95

Weight (kDa)

9.5

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 72 - 245 6.5e-88 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020141)

Species Orthologous Gene IDs
malus_domestica MD05G1249600.v1.1 MD10G1230000.v1.1
prunus_persica Prupe.4G114500_v2.0.a1 Prupe.4G114500_v2.0.a1
pyrus_communis pycom05g22690 pycom10g19360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 459
AccB7I CCANNNNNTGG 2 cut(s) 244, 380
AccI GTMKAC 2 cut(s) 483, 655
AccIII TCCGGA 1 cut(s) 776
AciI CCGC 1 cut(s) 37
AclWI GGATC 3 cut(s) 255, 596, 732
AfaI GTAC 7 cut(s) 70, 113, 261, 423, 612, 618, 748
AfiI CCNNNNNNNGG 3 cut(s) 244, 380, 485
AgsI TTSAA 5 cut(s) 82, 127, 368, 555, 683
AjiI CACGTC 1 cut(s) 757
AjnI CCWGG 2 cut(s) 431, 584
AloI GAACNNNNNNTCC 2 cut(s) 580, 612
AluBI AGCT 6 cut(s) 101, 463, 576, 701, 712, 725
AluI AGCT 6 cut(s) 101, 463, 576, 701, 712, 725
AlwI GGATC 3 cut(s) 255, 596, 732
Aor13HI TCCGGA 1 cut(s) 776
ApeKI GCWGC 3 cut(s) 101, 182, 460
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 764
AsuHPI GGTGA 1 cut(s) 407
AvaII GGWCC 1 cut(s) 764
BbsI GAAGAC 1 cut(s) 200
BbvI GCAGC 3 cut(s) 88, 169, 472
BccI CCATC 3 cut(s) 608, 629, 781
BciT130I CCWGG 2 cut(s) 433, 586
BciVI GTATCC 2 cut(s) 34, 297
BfaI CTAG 3 cut(s) 216, 425, 675
BfuI GTATCC 2 cut(s) 34, 297
BisI GCNGC 3 cut(s) 102, 183, 461
BlsI GCNGC 3 cut(s) 103, 184, 462
BmcAI AGTACT 1 cut(s) 423
Bme1390I CCNGG 2 cut(s) 433, 586
Bme18I GGWCC 1 cut(s) 764
BmgBI CACGTC 1 cut(s) 757
BmgT120I GGNCC 1 cut(s) 764
BmiI GGNNCC 2 cut(s) 42, 598
BmrFI CCNGG 2 cut(s) 433, 586
BmsI GCATC 2 cut(s) 479, 726
BpiI GAAGAC 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 736
BsaWI WCCGGW 1 cut(s) 776
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 380, 485
Bse1I ACTGG 2 cut(s) 186, 558
Bse3DI GCAATG 1 cut(s) 453
BseAI TCCGGA 1 cut(s) 776
BseBI CCWGG 2 cut(s) 433, 586
BseCI ATCGAT 1 cut(s) 736
BseGI GGATG 3 cut(s) 494, 717, 773
BseLI CCNNNNNNNGG 3 cut(s) 244, 380, 485
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 3 cut(s) 104, 495, 734
BseNI ACTGG 2 cut(s) 186, 558
BseXI GCAGC 3 cut(s) 88, 169, 472
BshVI ATCGAT 1 cut(s) 736
BsiSI CCGG 1 cut(s) 777
BslI CCNNNNNNNGG 3 cut(s) 244, 380, 485
BsmI GAATGC 1 cut(s) 503
Bsp13I TCCGGA 1 cut(s) 776
Bsp143I GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
BspACI CCGC 1 cut(s) 37
BspCNI CTCAG 3 cut(s) 103, 496, 735
BspDI ATCGAT 1 cut(s) 736
BspEI TCCGGA 1 cut(s) 776
BspLI GGNNCC 2 cut(s) 42, 598
BspPI GGATC 3 cut(s) 255, 596, 732
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 2 cut(s) 186, 558
BssMI GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
BssNAI GTATAC 1 cut(s) 484
Bst1107I GTATAC 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 433, 586
Bst4CI ACNGT 4 cut(s) 73, 116, 285, 545
BstAPI GCANNNNNTGC 2 cut(s) 466, 498
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 4 cut(s) 90, 267, 504, 743
BstF5I GGATG 3 cut(s) 494, 717, 773
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 7 cut(s) 110, 250, 309, 400, 591, 736, 740
BstMBI GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
BstMWI GCNNNNNNNGC 2 cut(s) 466, 498
BstNI CCWGG 2 cut(s) 433, 586
BstSCI CCNGG 2 cut(s) 431, 584
BstV1I GCAGC 3 cut(s) 88, 169, 472
BstV2I GAAGAC 1 cut(s) 200
BstZ17I GTATAC 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 736
BsuI GTATCC 2 cut(s) 34, 297
BsuTUI ATCGAT 1 cut(s) 736
BtrI CACGTC 1 cut(s) 757
BtsCI GGATG 3 cut(s) 494, 717, 773
BtsIMutI CAGTG 3 cut(s) 393, 492, 565
Cac8I GCNNGC 1 cut(s) 61
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 764
ClaI ATCGAT 1 cut(s) 736
CseI GACGC 1 cut(s) 666
Csp6I GTAC 7 cut(s) 69, 112, 260, 422, 611, 617, 747
CviAII CATG 3 cut(s) 137, 512, 668
CviJI RGCY 9 cut(s) 43, 101, 361, 463, 519, 576, 701, 712, 725
CviKI_1 RGCY 9 cut(s) 43, 101, 361, 463, 519, 576, 701, 712, 725
CviQI GTAC 7 cut(s) 69, 112, 260, 422, 611, 617, 747
DdeI CTNAG 4 cut(s) 90, 267, 504, 743
DpnI GATC 7 cut(s) 109, 249, 308, 399, 590, 735, 739
DpnII GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
Eco47I GGWCC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 431, 584
FaeI CATG 3 cut(s) 140, 515, 671
FatI CATG 3 cut(s) 136, 511, 667
FauI CCCGC 1 cut(s) 30
FblI GTMKAC 2 cut(s) 483, 655
Fnu4HI GCNGC 3 cut(s) 102, 183, 461
FokI GGATG 3 cut(s) 501, 704, 760
Fsp4HI GCNGC 3 cut(s) 102, 183, 461
FspBI CTAG 3 cut(s) 216, 425, 675
FspI TGCGCA 1 cut(s) 459
GlaI GCGC 1 cut(s) 459
GluI GCNGC 3 cut(s) 102, 183, 461
HapII CCGG 1 cut(s) 777
HgaI GACGC 1 cut(s) 666
HhaI GCGC 1 cut(s) 460
Hin1II CATG 3 cut(s) 140, 515, 671
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 1 cut(s) 656
HindII GTYRAC 1 cut(s) 656
HindIII AAGCTT 1 cut(s) 574
HinfI GANTC 4 cut(s) 10, 403, 428, 694
HpaII CCGG 1 cut(s) 777
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 3 cut(s) 254, 484, 656
Hpy188I TCNGA 3 cut(s) 51, 402, 505
Hpy188III TCNNGA 2 cut(s) 7, 777
Hpy8I GTNNAC 3 cut(s) 254, 484, 656
Hpy99I CGWCG 2 cut(s) 660, 761
HpyAV CCTTC 2 cut(s) 755, 761
HpyCH4III ACNGT 4 cut(s) 73, 116, 285, 545
HpyCH4IV ACGT 2 cut(s) 609, 756
HpyCH4V TGCA 6 cut(s) 104, 320, 341, 469, 492, 717
HpyF10VI GCNNNNNNNGC 2 cut(s) 466, 498
HpyF3I CTNAG 4 cut(s) 90, 267, 504, 743
HpySE526I ACGT 2 cut(s) 609, 756
Hsp92II CATG 3 cut(s) 140, 515, 671
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 776
Kzo9I GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
LmnI GCTCC 2 cut(s) 40, 709
Lsp1109I GCAGC 3 cut(s) 88, 169, 472
LweI GCATC 2 cut(s) 479, 726
MaeI CTAG 3 cut(s) 216, 425, 675
MaeII ACGT 2 cut(s) 609, 756
MalI GATC 7 cut(s) 109, 249, 308, 399, 590, 735, 739
MboI GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
MboII GAAGA 3 cut(s) 205, 289, 316
MluCI AATT 2 cut(s) 344, 444
MnlI CCTC 9 cut(s) 24, 37, 74, 99, 149, 319, 381, 499, 619
MroI TCCGGA 1 cut(s) 776
MseI TTAA 4 cut(s) 299, 443, 447, 663
MslI CAYNNNNRTG 1 cut(s) 778
MspA1I CMGCKG 3 cut(s) 101, 463, 701
MspI CCGG 1 cut(s) 777
MspR9I CCNGG 2 cut(s) 433, 586
Mva1269I GAATGC 1 cut(s) 503
MvaI CCWGG 2 cut(s) 433, 586
MwoI GCNNNNNNNGC 2 cut(s) 466, 498
NdeII GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
NlaIII CATG 3 cut(s) 140, 515, 671
NlaIV GGNNCC 2 cut(s) 42, 598
NsbI TGCGCA 1 cut(s) 459
PacI TTAATTAA 1 cut(s) 447
PctI GAATGC 1 cut(s) 503
PfeI GAWTC 4 cut(s) 10, 403, 428, 694
PflMI CCANNNNNTGG 2 cut(s) 244, 380
PfoI TCCNGGA 2 cut(s) 431, 584
PkrI GCNGC 3 cut(s) 103, 184, 462
Psp6I CCWGG 2 cut(s) 431, 584
PspGI CCWGG 2 cut(s) 431, 584
PspN4I GGNNCC 2 cut(s) 42, 598
PspPI GGNCC 1 cut(s) 764
PvuII CAGCTG 3 cut(s) 101, 463, 701
RsaI GTAC 7 cut(s) 70, 113, 261, 423, 612, 618, 748
RsaNI GTAC 7 cut(s) 69, 112, 260, 422, 611, 617, 747
RseI CAYNNNNRTG 1 cut(s) 778
SalI GTCGAC 1 cut(s) 654
SaqAI TTAA 4 cut(s) 299, 443, 447, 663
SatI GCNGC 3 cut(s) 102, 183, 461
Sau3AI GATC 7 cut(s) 107, 247, 306, 397, 588, 733, 737
Sau96I GGNCC 1 cut(s) 764
ScaI AGTACT 1 cut(s) 423
ScrFI CCNGG 2 cut(s) 433, 586
SfaNI GCATC 2 cut(s) 479, 726
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 778
Sse9I AATT 2 cut(s) 344, 444
SsiI CCGC 1 cut(s) 37
SspMI CTAG 3 cut(s) 216, 425, 675
StyD4I CCNGG 2 cut(s) 431, 584
TaaI ACNGT 4 cut(s) 73, 116, 285, 545
TaiI ACGT 2 cut(s) 612, 759
TaqI TCGA 4 cut(s) 624, 655, 736, 759
TasI AATT 2 cut(s) 344, 444
TatI WGTACW 2 cut(s) 259, 421
TfiI GAWTC 4 cut(s) 10, 403, 428, 694
Tru1I TTAA 4 cut(s) 299, 443, 447, 663
Tru9I TTAA 4 cut(s) 299, 443, 447, 663
TscAI CASTG 3 cut(s) 400, 499, 565
TseI GCWGC 3 cut(s) 101, 182, 460
TspDTI ATGAA 3 cut(s) 137, 500, 684
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 3 cut(s) 400, 499, 565
Van91I CCANNNNNTGG 2 cut(s) 244, 380
VpaK11BI GGWCC 1 cut(s) 764
XmiI GTMKAC 2 cut(s) 483, 655
XspI CTAG 3 cut(s) 216, 425, 675
ZrmI AGTACT 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.