Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0008054

Physical Location & Seq

Genomic Coordinates Reverse (-)

22691107 .. 22694015

Gene Structure 2909 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGTATAAACGAGAAAAATCAGAAAGACGAGAAATTGATAGCAAAGCCCCGCCCCCTCCACCAAAAGCAGAGAGCAGAGAAACAGAGAGACCGGAGAGAGAAGCGAAAATGGGGAAGACAAGCTACAAGAAGCTAACCGGAAGCCAAAACCTAAGACAGCGGCTCGTACTGGCGGCGCTTGCGTCCACACCGGTTCTTATCGAGGACATTCGCGCAGAGGAGACGTGGCCCGGCCTCCGCCCCCACGAGGTCTCCTTCCTCCGCCTCCTCGAGAAGGTCTCCGATGACTGCGTCGTGGAAATCAACGAGACTGGTACGAAATTGAAGTTCAAGCCGGGGATAGTGATGGGTGGGAGGAATTTGGTGCACGACTGCGGGCTCAGTCGGGCGATTGGGTATTTCTTGGAGCCATTGATTGTGCTTGGTCTCTTTGCCAAAAAACCGCTTACCATTACGCTTAAAGGAATTACAAATGATCCCAAGGACCCATCAGTTGATACGTTCCGGTCCACTACCTTACCCATGTTAAAACACTTTGGAGTTCCCTCAGAAGGGCTAGACCTTAAAATACAGAGTCGTGGATCTGCTCCTCAAGGTGGCGGTGAAGTGTTTTTGTCAATCCCGATTGTTCAAAGTCTAACTGCAGTAAACTGGATTGATGAGGGGATGGTTAAGAGGATTAGAGGGGTTACGTTCTCAACAAGAGTGTCCACGCAGTTTGAAAATACCATGGTGTATGCCGCGCGTGGAATCTTTAATCTCTTACTCCCAGATGTTCACATATTTACTGACCATAGAAGTGGCGCACAAGCTGGACTTTCACCTGGTTATGGAATTTCACTGGTTGCGGAAACAACTTCTGGTTGCTGTATCTCTGCTGATACAGCAATTTCTTACTCTCGAGGGGAAGAAACAGTTGAACTTGAAGATGAGGAGAAGAAAGATCTTATGCCTCCAGAGGATGTTGGTGTGCAAATGGCTAATGTTCTGCTTGGGGAGATTGAGCAGGGTGGAGTGGTAGATTCAACACATCAGGGCTTGTTGTTTCTTCTCTGTGCACTATGTCCACAGGATGTTTCAAAGGTTCGTGTTGGAAAGCTCTCTCCTTATGGCATAGAAACACTCAGAAACATCAAGGACTTTCTCGGTGTTAAATTTGTCATTAAGCCCGACCCATCCACAGGGACTGTTATACTTAAATGCGTTGGCTCTGGGTTAAGGAACCTATCGAGAAGGATCTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000469 GO:0000478 GO:0000479 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0016787 GO:0016788 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

RTC RTC_insert

Protein Analysis

415

Amino Acids

45.33

Weight (kDa)

8.07

Isoelectric Point (pI)

42.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RTC	PF01137	46 - 388	2.3e-59	RNA 3'-terminal phosphate cyclase
RTC_insert	PF05189	220 - 335	2.1e-27	RNA 3'-terminal phosphate cyclase (RTC), insert domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008054)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22100
fragaria_vesca	FvH4_3g12140 FvH4_3g12140 FvH4_3g12140 FvH4_3g12140 FvH4_3g12140 FvH4_3g12140
malus_domestica	MD05G1254500.v1.1 MD10G1234700.v1.1
prunus_persica	Prupe.4G110100_v2.0.a1
pyrus_communis	pycom05g23230 pycom10g19650
rosa_chinensis	RchiOBHm_Chr5g0019871
rosa_laevigata	RLG00000032480
rosa_multiflora	Rmu_sc0000344.1_g000019 Rmu_sc0001319.1_g000005
rosa_roxburghii	Rroxscaffold_1G00058300
rosa_rugosa	Rorug05G0054600.1 Rorug05G0054700
rosa_samantha	Rh5AG144800 Rh5BG144000 Rh5CG155700 Rh5DG143700
rosa_wichuraiana	Rw5G012790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	213, 743, 745
AclWI	GGATC	3 cut(s)	470, 589, 1244
AcsI	RAATTY	3 cut(s)	358, 834, 1154
AfaI	GTAC	2 cut(s)	168, 316
AfiI	CCNNNNNNNGG	7 cut(s)	247, 274, 551, 552, 596, 830, 1181
AgeI	ACCGGT	1 cut(s)	190
AgsI	TTSAA	8 cut(s)	325, 331, 632, 722, 920, 926, 1026, 1080
AjiI	CACGTC	1 cut(s)	225
AjnI	CCWGG	1 cut(s)	823
AluBI	AGCT	4 cut(s)	123, 133, 812, 1099
AluI	AGCT	4 cut(s)	123, 133, 812, 1099
Alw21I	GWGCWC	2 cut(s)	369, 1060
Alw26I	GTCTC	6 cut(s)	82, 215, 256, 283, 302, 431
Alw44I	GTGCAC	2 cut(s)	365, 1056
AlwI	GGATC	3 cut(s)	470, 589, 1244
AlwNI	CAGNNNCTG	1 cut(s)	1187
Ama87I	CYCGRG	2 cut(s)	269, 900
AoxI	GGCC	2 cut(s)	227, 232
ApaLI	GTGCAC	2 cut(s)	365, 1056
ApoI	RAATTY	3 cut(s)	358, 834, 1154
AsiGI	ACCGGT	1 cut(s)	190
AspLEI	GCGC	4 cut(s)	178, 215, 745, 806
AspS9I	GGNCC	3 cut(s)	228, 484, 507
AsuC2I	CCSGG	2 cut(s)	231, 336
AsuHPI	GGTGA	2 cut(s)	614, 813
AvaI	CYCGRG	2 cut(s)	269, 900
AvaII	GGWCC	2 cut(s)	484, 507
BaeGI	GKGCMC	2 cut(s)	369, 1060
BanII	GRGCYC	1 cut(s)	381
BarI	GAAGNNNNNNTAC	2 cut(s)	107, 139
BauI	CACGAG	1 cut(s)	245
BbsI	GAAGAC	1 cut(s)	122
Bbv12I	GWGCWC	2 cut(s)	369, 1060
BccI	CCATC	4 cut(s)	340, 496, 661, 1183
BciT130I	CCWGG	1 cut(s)	825
BcnI	CCSGG	2 cut(s)	231, 336
BcoDI	GTCTC	6 cut(s)	82, 215, 256, 283, 302, 431
BfaI	CTAG	1 cut(s)	557
BfmI	CTRYAG	1 cut(s)	642
BfoI	RGCGCY	1 cut(s)	179
BglII	AGATCT	1 cut(s)	943
BisI	GCNGC	3 cut(s)	161, 174, 741
BlsI	GCNGC	3 cut(s)	162, 175, 742
Bme1390I	CCNGG	3 cut(s)	231, 336, 825
Bme18I	GGWCC	2 cut(s)	484, 507
BmeT110I	CYCGRG	2 cut(s)	269, 900
BmgBI	CACGTC	1 cut(s)	225
BmgT120I	GGNCC	3 cut(s)	228, 484, 507
BmiI	GGNNCC	3 cut(s)	408, 486, 1223
BmrFI	CCNGG	3 cut(s)	231, 336, 825
BpiI	GAAGAC	1 cut(s)	122
BplI	GAGNNNNNCTC	2 cut(s)	263, 295
BpmI	CTGGAG	1 cut(s)	939
BpuEI	CTTGAG	1 cut(s)	576
BpuMI	CCSGG	2 cut(s)	231, 336
BsaI	GGTCTC	4 cut(s)	82, 256, 283, 431
BsaJI	CCNNGG	3 cut(s)	335, 480, 729
BsaWI	WCCGGW	4 cut(s)	91, 137, 190, 504
BsaXI	ACNNNNNCTCC	2 cut(s)	236, 266
Bsc4I	CCNNNNNNNGG	7 cut(s)	247, 274, 551, 552, 596, 830, 1181
Bse118I	RCCGGY	1 cut(s)	190
Bse1I	ACTGG	4 cut(s)	174, 316, 656, 846
BseBI	CCWGG	1 cut(s)	825
BseDI	CCNNGG	3 cut(s)	335, 480, 729
BseGI	GGATG	4 cut(s)	672, 967, 1078, 1175
BseLI	CCNNNNNNNGG	7 cut(s)	247, 274, 551, 552, 596, 830, 1181
BseMII	CTCAG	3 cut(s)	394, 561, 1138
BseNI	ACTGG	4 cut(s)	174, 316, 656, 846
BseRI	GAGGAG	4 cut(s)	233, 257, 579, 947
BseSI	GKGCMC	2 cut(s)	369, 1060
Bsh1236I	CGCG	3 cut(s)	213, 743, 745
BshFI	GGCC	2 cut(s)	229, 234
BshTI	ACCGGT	1 cut(s)	190
BsiHKAI	GWGCWC	2 cut(s)	369, 1060
BsiHKCI	CYCGRG	2 cut(s)	269, 900
BsiSI	CCGG	6 cut(s)	92, 138, 191, 231, 335, 505
BslFI	GGGAC	1 cut(s)	1198
BslI	CCNNNNNNNGG	7 cut(s)	247, 274, 551, 552, 596, 830, 1181
BsmAI	GTCTC	6 cut(s)	82, 215, 256, 283, 302, 431
BsmBI	CGTCTC	1 cut(s)	215
BsmFI	GGGAC	1 cut(s)	1198
BsnI	GGCC	2 cut(s)	229, 234
Bso31I	GGTCTC	4 cut(s)	82, 256, 283, 431
BsoBI	CYCGRG	2 cut(s)	269, 900
Bsp1286I	GDGCHC	3 cut(s)	369, 381, 1060
Bsp143I	GATC	4 cut(s)	475, 581, 943, 1236
Bsp19I	CCATGG	1 cut(s)	729
BspANI	GGCC	2 cut(s)	229, 234
BspCNI	CTCAG	3 cut(s)	393, 560, 1137
BspFNI	CGCG	3 cut(s)	213, 743, 745
BspHI	TCATGA	1 cut(s)	1241
BspLI	GGNNCC	3 cut(s)	408, 486, 1223
BspMAI	CTGCAG	1 cut(s)	646
BspPI	GGATC	3 cut(s)	470, 589, 1244
BspTNI	GGTCTC	4 cut(s)	82, 256, 283, 431
BsrFI	RCCGGY	1 cut(s)	190
BsrI	ACTGG	4 cut(s)	174, 316, 656, 846
BssAI	RCCGGY	1 cut(s)	190
BssECI	CCNNGG	3 cut(s)	335, 480, 729
BssMI	GATC	4 cut(s)	475, 581, 943, 1236
BssSI	CACGAG	1 cut(s)	245
BssT1I	CCWWGG	2 cut(s)	480, 729
Bst2BI	CACGAG	1 cut(s)	245
Bst2UI	CCWGG	1 cut(s)	825
Bst4CI	ACNGT	2 cut(s)	916, 1189
BstC8I	GCNNGC	2 cut(s)	180, 377
BstDEI	CTNAG	4 cut(s)	152, 380, 547, 1124
BstDSI	CCRYGG	1 cut(s)	729
BstENI	CCTNNNNNAGG	1 cut(s)	272
BstF5I	GGATG	4 cut(s)	672, 967, 1078, 1175
BstFNI	CGCG	3 cut(s)	213, 743, 745
BstH2I	RGCGCY	1 cut(s)	179
BstHHI	GCGC	4 cut(s)	178, 215, 745, 806
BstKTI	GATC	4 cut(s)	478, 584, 946, 1239
BstMAI	GTCTC	6 cut(s)	82, 215, 256, 283, 302, 431
BstMBI	GATC	4 cut(s)	475, 581, 943, 1236
BstMWI	GCNNNNNNNGC	2 cut(s)	179, 884
BstNI	CCWGG	1 cut(s)	825
BstSCI	CCNGG	3 cut(s)	229, 334, 823
BstSFI	CTRYAG	1 cut(s)	642
BstSLI	GKGCMC	2 cut(s)	369, 1060
BstUI	CGCG	3 cut(s)	213, 743, 745
BstV2I	GAAGAC	1 cut(s)	122
BstX2I	RGATCY	3 cut(s)	581, 943, 1236
BstXI	CCANNNNNNTGG	1 cut(s)	800
BstYI	RGATCY	3 cut(s)	581, 943, 1236
BsuRI	GGCC	2 cut(s)	229, 234
BtgI	CCRYGG	1 cut(s)	729
BtrI	CACGTC	1 cut(s)	225
BtsCI	GGATG	4 cut(s)	672, 967, 1078, 1175
BtsIMutI	CAGTG	1 cut(s)	839
Cac8I	GCNNGC	2 cut(s)	180, 377
CaiI	CAGNNNCTG	1 cut(s)	1187
CciI	TCATGA	1 cut(s)	1241
CfoI	GCGC	4 cut(s)	178, 215, 745, 806
Cfr10I	RCCGGY	1 cut(s)	190
Cfr13I	GGNCC	3 cut(s)	228, 484, 507
CseI	GACGC	2 cut(s)	171, 280
CsiI	ACCWGGT	1 cut(s)	823
Csp6I	GTAC	2 cut(s)	167, 315
CspAI	ACCGGT	1 cut(s)	190
CspCI	CAANNNNNGTGG	2 cut(s)	700, 735
CviAII	CATG	3 cut(s)	523, 730, 1242
CviQI	GTAC	2 cut(s)	167, 315
DdeI	CTNAG	4 cut(s)	152, 380, 547, 1124
DpnI	GATC	4 cut(s)	477, 583, 945, 1238
DpnII	GATC	4 cut(s)	475, 581, 943, 1236
EciI	GGCGGA	2 cut(s)	227, 251
Eco130I	CCWWGG	2 cut(s)	480, 729
Eco24I	GRGCYC	1 cut(s)	381
Eco31I	GGTCTC	4 cut(s)	82, 256, 283, 431
Eco47I	GGWCC	2 cut(s)	484, 507
Eco88I	CYCGRG	2 cut(s)	269, 900
EcoNI	CCTNNNNNAGG	1 cut(s)	272
EcoO109I	RGGNCCY	1 cut(s)	484
EcoRII	CCWGG	1 cut(s)	823
EcoT14I	CCWWGG	2 cut(s)	480, 729
EcoT38I	GRGCYC	1 cut(s)	381
ErhI	CCWWGG	2 cut(s)	480, 729
Esp3I	CGTCTC	1 cut(s)	215
FaeI	CATG	3 cut(s)	526, 733, 1245
FalI	AAGNNNNNCTT	2 cut(s)	801, 833
FaqI	GGGAC	1 cut(s)	1198
FatI	CATG	3 cut(s)	522, 729, 1241
FauI	CCCGC	2 cut(s)	57, 368
Fnu4HI	GCNGC	3 cut(s)	161, 174, 741
FokI	GGATG	4 cut(s)	679, 974, 1085, 1162
FriOI	GRGCYC	1 cut(s)	381
Fsp4HI	GCNGC	3 cut(s)	161, 174, 741
FspBI	CTAG	1 cut(s)	557
GlaI	GCGC	4 cut(s)	177, 214, 744, 805
GluI	GCNGC	3 cut(s)	161, 174, 741
GsuI	CTGGAG	1 cut(s)	939
HaeII	RGCGCY	1 cut(s)	179
HaeIII	GGCC	2 cut(s)	229, 234
HapII	CCGG	6 cut(s)	92, 138, 191, 231, 335, 505
HgaI	GACGC	2 cut(s)	171, 280
HhaI	GCGC	4 cut(s)	178, 215, 745, 806
Hin1II	CATG	3 cut(s)	526, 733, 1245
Hin6I	GCGC	4 cut(s)	176, 213, 743, 804
HinP1I	GCGC	4 cut(s)	176, 213, 743, 804
HinfI	GANTC	3 cut(s)	574, 750, 1022
HpaII	CCGG	6 cut(s)	92, 138, 191, 231, 335, 505
HphI	GGTGA	2 cut(s)	614, 813
Hpy166II	GTNNAC	8 cut(s)	186, 367, 510, 649, 711, 778, 1058, 1067
Hpy188I	TCNGA	4 cut(s)	22, 283, 550, 1127
Hpy188III	TCNNGA	6 cut(s)	271, 622, 900, 956, 1230, 1242
Hpy8I	GTNNAC	8 cut(s)	186, 367, 510, 649, 711, 778, 1058, 1067
Hpy99I	CGWCG	1 cut(s)	296
HpyAV	CCTTC	4 cut(s)	265, 268, 545, 1227
HpyCH4III	ACNGT	2 cut(s)	916, 1189
HpyCH4IV	ACGT	3 cut(s)	224, 500, 692
HpyCH4V	TGCA	4 cut(s)	367, 644, 973, 1058
HpyF10VI	GCNNNNNNNGC	2 cut(s)	179, 884
HpyF3I	CTNAG	4 cut(s)	152, 380, 547, 1124
HpySE526I	ACGT	3 cut(s)	224, 500, 692
Hsp92II	CATG	3 cut(s)	526, 733, 1245
HspAI	GCGC	4 cut(s)	176, 213, 743, 804
Kzo9I	GATC	4 cut(s)	475, 581, 943, 1236
LmnI	GCTCC	2 cut(s)	406, 592
MabI	ACCWGGT	1 cut(s)	823
MaeI	CTAG	1 cut(s)	557
MaeII	ACGT	3 cut(s)	224, 500, 692
MaeIII	GTNAC	1 cut(s)	688
MalI	GATC	4 cut(s)	477, 583, 945, 1238
MboI	GATC	4 cut(s)	475, 581, 943, 1236
MboII	GAAGA	5 cut(s)	127, 920, 938, 949, 1040
MflI	RGATCY	3 cut(s)	581, 943, 1236
MhlI	GDGCHC	3 cut(s)	369, 381, 1060
MluCI	AATT	7 cut(s)	33, 320, 358, 465, 834, 888, 1154
MlyI	GAGTC	1 cut(s)	583
MmeI	TCCRAC	1 cut(s)	1072
MseI	TTAA	9 cut(s)	459, 527, 564, 672, 756, 1152, 1164, 1197, 1217
MslI	CAYNNNNRTG	1 cut(s)	798
MspA1I	CMGCKG	1 cut(s)	160
MspI	CCGG	6 cut(s)	92, 138, 191, 231, 335, 505
MspR9I	CCNGG	3 cut(s)	231, 336, 825
MvaI	CCWGG	1 cut(s)	825
MvnI	CGCG	3 cut(s)	213, 743, 745
MwoI	GCNNNNNNNGC	2 cut(s)	179, 884
NciI	CCSGG	2 cut(s)	231, 336
NcoI	CCATGG	1 cut(s)	729
NdeII	GATC	4 cut(s)	475, 581, 943, 1236
NlaIII	CATG	3 cut(s)	526, 733, 1245
NlaIV	GGNNCC	3 cut(s)	408, 486, 1223
PaeR7I	CTCGAG	2 cut(s)	269, 900
PagI	TCATGA	1 cut(s)	1241
PfeI	GAWTC	2 cut(s)	750, 1022
PflFI	GACNNNGTC	1 cut(s)	290
PinAI	ACCGGT	1 cut(s)	190
PkrI	GCNGC	3 cut(s)	162, 175, 742
PleI	GAGTC	1 cut(s)	582
PpsI	GAGTC	1 cut(s)	582
PpuMI	RGGWCCY	1 cut(s)	484
Psp5II	RGGWCCY	1 cut(s)	484
Psp6I	CCWGG	1 cut(s)	823
PspGI	CCWGG	1 cut(s)	823
PspN4I	GGNNCC	3 cut(s)	408, 486, 1223
PspPI	GGNCC	3 cut(s)	228, 484, 507
PspPPI	RGGWCCY	1 cut(s)	484
PstI	CTGCAG	1 cut(s)	646
PstNI	CAGNNNCTG	1 cut(s)	1187
PsuI	RGATCY	3 cut(s)	581, 943, 1236
PsyI	GACNNNGTC	1 cut(s)	290
RsaI	GTAC	2 cut(s)	168, 316
RsaNI	GTAC	2 cut(s)	167, 315
RseI	CAYNNNNRTG	1 cut(s)	798
SaqAI	TTAA	9 cut(s)	459, 527, 564, 672, 756, 1152, 1164, 1197, 1217
SatI	GCNGC	3 cut(s)	161, 174, 741
Sau3AI	GATC	4 cut(s)	475, 581, 943, 1236
Sau96I	GGNCC	3 cut(s)	228, 484, 507
SchI	GAGTC	1 cut(s)	583
ScrFI	CCNGG	3 cut(s)	231, 336, 825
SduI	GDGCHC	3 cut(s)	369, 381, 1060
SexAI	ACCWGGT	1 cut(s)	823
SfcI	CTRYAG	1 cut(s)	642
Sfr274I	CTCGAG	2 cut(s)	269, 900
SinI	GGWCC	2 cut(s)	484, 507
SlaI	CTCGAG	2 cut(s)	269, 900
SmiMI	CAYNNNNRTG	1 cut(s)	798
SmlI	CTYRAG	3 cut(s)	269, 591, 900
SmoI	CTYRAG	3 cut(s)	269, 591, 900
Sse9I	AATT	7 cut(s)	33, 320, 358, 465, 834, 888, 1154
SspMI	CTAG	1 cut(s)	557
StyD4I	CCNGG	3 cut(s)	229, 334, 823
StyI	CCWWGG	2 cut(s)	480, 729
TaaI	ACNGT	2 cut(s)	916, 1189
TaiI	ACGT	3 cut(s)	227, 503, 695
TaqI	TCGA	4 cut(s)	201, 270, 901, 1229
TasI	AATT	7 cut(s)	33, 320, 358, 465, 834, 888, 1154
TauI	GCSGC	3 cut(s)	163, 176, 743
TfiI	GAWTC	2 cut(s)	750, 1022
Tru1I	TTAA	9 cut(s)	459, 527, 564, 672, 756, 1152, 1164, 1197, 1217
Tru9I	TTAA	9 cut(s)	459, 527, 564, 672, 756, 1152, 1164, 1197, 1217
TscAI	CASTG	1 cut(s)	846
TspRI	CASTG	1 cut(s)	846
Tth111I	GACNNNGTC	1 cut(s)	290
VneI	GTGCAC	2 cut(s)	365, 1056
VpaK11BI	GGWCC	2 cut(s)	484, 507
XagI	CCTNNNNNAGG	1 cut(s)	272
XapI	RAATTY	3 cut(s)	358, 834, 1154
XhoI	CTCGAG	2 cut(s)	269, 900
XspI	CTAG	1 cut(s)	557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.