pycom10g24930

U2 snRNP auxiliary factor large subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0020969
Physical Location & Seq
Forward (+)
26657408 .. 26658844
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g24930.5

Sequence Viewer

Length: 540 bp
ATGGGCTTAAATTTCCAACCTTTCCATGGTTTTGACCTCGTGAAGGATAATGATACAGGAAACTCTAAAGGATATGGATTCTGTGTTTATCAGTGCCAGAAAATGGCCATGCAGGTTGTTGACTTGAATCTTCTAGGAGCTGGAATGGCAACTATGGCGAGTGGTGAGACACCCACTAAGGTCCTATGCTTGACTGAGGCGATTACTGCTGATCAACTGGGTGACGATGAAGAGTATGTCGAAATATTAGAAGACATGAGGGATGAATGCAGCAAATTTGGAACTCTTGTGAATGTTGTTATTCCTCGTGCGGATCAAGGCGGAGAACAGATTCCAGGTGTTGGGAAGGTGTTCTTGGAATATACGGATATTGGGGGTTGCGCCAATGCGAAGAATGTACTCGGTGGGAGAAAATTCGGAGGTAACAGTCAATGCCGTTTACTATCCAGAAGAGAAATACTATATCAGGGACTACAGTGCATAAGAGTACTAAGCCCCCCCGGCCTAGGTTTTTATCGTTTTTGTTGCAATGTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1

Protein Analysis

180

Amino Acids

19.53

Weight (kDa)

5.04

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60830 AT1G60830
pyrus_communis pycom10g24930
rosa_multiflora Rmu_co8118650.1_g000001 Rmu_sc0039049.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 103
AccB7I CCANNNNNTGG 2 cut(s) 103, 341
AciI CCGC 2 cut(s) 311, 321
AclWI GGATC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 3 cut(s) 10, 275, 413
AfaI GTAC 2 cut(s) 399, 489
AfiI CCNNNNNNNGG 5 cut(s) 26, 43, 103, 341, 506
AgsI TTSAA 1 cut(s) 127
AjnI CCWGG 1 cut(s) 334
AjuI GAANNNNNNNTTGG 2 cut(s) 338, 370
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 321
AoxI GGCC 2 cut(s) 105, 502
ApeKI GCWGC 1 cut(s) 270
ApoI RAATTY 3 cut(s) 10, 275, 413
Asp700I GAANNNNTTC 2 cut(s) 330, 350
AspA2I CCTAGG 1 cut(s) 505
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 2 cut(s) 176, 233
AvaII GGWCC 1 cut(s) 181
AvrII CCTAGG 1 cut(s) 505
BalI TGGCCA 1 cut(s) 107
BauI CACGAG 2 cut(s) 38, 306
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 420
BciT130I CCWGG 1 cut(s) 336
BclI TGATCA 1 cut(s) 211
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 134, 506
BfmI CTRYAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 103
BglI GCCNNNNNGGC 1 cut(s) 501
BisI GCNGC 1 cut(s) 271
BlnI CCTAGG 1 cut(s) 505
BlsI GCNGC 1 cut(s) 272
BmcAI AGTACT 1 cut(s) 489
Bme1390I CCNGG 2 cut(s) 336, 501
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 336, 501
BmrI ACTGGG 1 cut(s) 227
BmuI ACTGGG 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 25, 499, 505
Bsc4I CCNNNNNNNGG 5 cut(s) 26, 43, 103, 341, 506
Bse1I ACTGG 1 cut(s) 222
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 25, 499, 505
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 5 cut(s) 26, 43, 103, 341, 506
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 282
BshFI GGCC 2 cut(s) 107, 504
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 5 cut(s) 26, 43, 103, 341, 506
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 2 cut(s) 107, 504
Bsp143I GATC 2 cut(s) 211, 313
Bsp19I CCATGG 1 cut(s) 25
BspACI CCGC 2 cut(s) 311, 321
BspANI GGCC 2 cut(s) 107, 504
BspCNI CTCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 103
BspPI GGATC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 535
BsrI ACTGG 1 cut(s) 222
BssECI CCNNGG 3 cut(s) 25, 499, 505
BssMI GATC 2 cut(s) 211, 313
BssSI CACGAG 2 cut(s) 38, 306
BssT1I CCWWGG 2 cut(s) 25, 505
Bst2BI CACGAG 2 cut(s) 38, 306
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 428, 477
Bst6I CTCTTC 2 cut(s) 225, 445
BstDEI CTNAG 3 cut(s) 177, 195, 491
BstDSI CCRYGG 1 cut(s) 25
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 268
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 2 cut(s) 214, 316
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 211, 313
BstMWI GCNNNNNNNGC 4 cut(s) 146, 155, 206, 501
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 334, 499
BstSFI CTRYAG 1 cut(s) 473
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 258
BsuRI GGCC 2 cut(s) 107, 504
BtgI CCRYGG 1 cut(s) 25
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 98, 482
BveI ACCTGC 1 cut(s) 103
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 2 cut(s) 398, 488
CviAII CATG 3 cut(s) 26, 109, 256
CviJI RGCY 5 cut(s) 6, 107, 140, 495, 504
CviKI_1 RGCY 5 cut(s) 6, 107, 140, 495, 504
CviQI GTAC 2 cut(s) 398, 488
DdeI CTNAG 3 cut(s) 177, 195, 491
DpnI GATC 2 cut(s) 213, 315
DpnII GATC 2 cut(s) 211, 313
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 2 cut(s) 225, 445
EarI CTCTTC 2 cut(s) 225, 445
EciI GGCGGA 1 cut(s) 336
Eco130I CCWWGG 2 cut(s) 25, 505
Eco47I GGWCC 1 cut(s) 181
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 25, 505
ErhI CCWWGG 2 cut(s) 25, 505
FaeI CATG 3 cut(s) 29, 112, 259
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 1 cut(s) 483
FatI CATG 3 cut(s) 25, 108, 255
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 271
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 271
FspBI CTAG 2 cut(s) 134, 506
GlaI GCGC 1 cut(s) 382
GluI GCNGC 1 cut(s) 271
HaeIII GGCC 2 cut(s) 107, 504
HapII CCGG 1 cut(s) 501
HhaI GCGC 1 cut(s) 383
Hin1II CATG 3 cut(s) 29, 112, 259
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 3 cut(s) 78, 127, 331
HpaII CCGG 1 cut(s) 501
HphI GGTGA 2 cut(s) 176, 233
Hpy166II GTNNAC 2 cut(s) 121, 440
Hpy188I TCNGA 1 cut(s) 419
Hpy188III TCNNGA 2 cut(s) 40, 447
Hpy8I GTNNAC 2 cut(s) 121, 440
HpyAV CCTTC 2 cut(s) 37, 340
HpyCH4III ACNGT 2 cut(s) 428, 477
HpyCH4V TGCA 4 cut(s) 112, 270, 480, 528
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 155, 206, 501
HpyF3I CTNAG 3 cut(s) 177, 195, 491
Hsp92II CATG 3 cut(s) 29, 112, 259
HspAI GCGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 2 cut(s) 211, 313
LmnI GCTCC 1 cut(s) 137
Lsp1109I GCAGC 1 cut(s) 282
MaeI CTAG 2 cut(s) 134, 506
MaeIII GTNAC 2 cut(s) 221, 422
MalI GATC 2 cut(s) 213, 315
MboI GATC 2 cut(s) 211, 313
MboII GAAGA 5 cut(s) 122, 242, 263, 403, 462
MlsI TGGCCA 1 cut(s) 107
MluCI AATT 3 cut(s) 10, 275, 413
MluNI TGGCCA 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 5 cut(s) 47, 190, 252, 315, 413
Mox20I TGGCCA 1 cut(s) 107
MroXI GAANNNNTTC 2 cut(s) 330, 350
MscI TGGCCA 1 cut(s) 107
MseI TTAA 1 cut(s) 8
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 2 cut(s) 336, 501
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 146, 155, 206, 501
NciI CCSGG 1 cut(s) 501
NcoI CCATGG 1 cut(s) 25
NdeII GATC 2 cut(s) 211, 313
NlaIII CATG 3 cut(s) 29, 112, 259
NmuCI GTSAC 1 cut(s) 221
PctI GAATGC 1 cut(s) 272
PdmI GAANNNNTTC 2 cut(s) 330, 350
PfeI GAWTC 3 cut(s) 78, 127, 331
PflMI CCANNNNNTGG 2 cut(s) 103, 341
PkrI GCNGC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspPI GGNCC 1 cut(s) 181
PspPPI RGGWCCY 1 cut(s) 181
RsaI GTAC 2 cut(s) 399, 489
RsaNI GTAC 2 cut(s) 398, 488
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 1 cut(s) 271
Sau3AI GATC 2 cut(s) 211, 313
Sau96I GGNCC 1 cut(s) 181
ScaI AGTACT 1 cut(s) 489
ScrFI CCNGG 2 cut(s) 336, 501
SetI ASST 9 cut(s) 22, 39, 117, 142, 183, 340, 351, 424, 511
SfcI CTRYAG 1 cut(s) 473
SinI GGWCC 1 cut(s) 181
Sse9I AATT 3 cut(s) 10, 275, 413
SsiI CCGC 2 cut(s) 311, 321
SspI AATATT 1 cut(s) 246
SspMI CTAG 2 cut(s) 134, 506
StyD4I CCNGG 2 cut(s) 334, 499
StyI CCWWGG 2 cut(s) 25, 505
TaaI ACNGT 2 cut(s) 428, 477
TaqI TCGA 1 cut(s) 240
TasI AATT 3 cut(s) 10, 275, 413
TatI WGTACW 2 cut(s) 397, 487
TfiI GAWTC 3 cut(s) 78, 127, 331
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 2 cut(s) 98, 482
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 1 cut(s) 270
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 2 cut(s) 243, 279
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 2 cut(s) 98, 482
Van91I CCANNNNNTGG 2 cut(s) 103, 341
VpaK11BI GGWCC 1 cut(s) 181
XagI CCTNNNNNAGG 1 cut(s) 41
XapI RAATTY 3 cut(s) 10, 275, 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 23
XmaJI CCTAGG 1 cut(s) 505
XmnI GAANNNNTTC 2 cut(s) 330, 350
XspI CTAG 2 cut(s) 134, 506
ZrmI AGTACT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.