pycom10g25480

Component of the ESCRT-I complex (endosomal sorting complex required for transport I), a regulator of vesicular trafficking process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006238
Physical Location & Seq
Forward (+)
27112653 .. 27113282
630 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGGAGGTCAAGCTGGCCAATGACAAGCGTGAAAGAGAAATGTATGATAGTTTTGCCGAACTATACGCCATTATCAAGACCACTGAGAAGCTTGAAAAGGTGTATGTTCGGGATGTGATCACTTCTTCTGCGTACGAGGCTGAATGCCAAAAACTTATTGCCCACTTTAAGACATTGGCATCCACGCTAAAGGACACCATCCCAAGCATCGAGCGGTTTGCAGATACATACAAGATGGACTGCCCAGCAGCTATAAACCGCCTTGTGACCTCAGGGGTGCCTGCCACAGTGGAGCACCGGGCTGCTGCAGCTTCCTCCTTGACTTCCTCAGCTGCTGCTGTGGCAGAATGCACACAGAATTTCATTACTGCAATGGACTCCTTGAAGCTGAACATGGTGGCAGTTGATCAGGTGCACCCGCTGCTCTCGGACCTCTCAACGTCCCTCGGGAAGTTAACCTTTCTGCCACCGGACTTTTTGGGAAAGGTGAAGTTGAAGGAATGGATTGCAAGGCTGTCGAAGATGGGAGCAGCCGATGAGTTGACGGAGCAGCAGTCAAGGCAGCTTCACTTTGATCTCGAGTCATCTTACAACTCGTTTATGTCAGCTTTGCCGAATTATGGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
VPS28

Protein Analysis

210

Amino Acids

23.16

Weight (kDa)

5.62

Isoelectric Point (pI)

32.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS28 PF03997 18 - 204 3.9e-71 VPS28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 277
AccBSI CCGCTC 1 cut(s) 214
AciI CCGC 3 cut(s) 214, 259, 419
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 2 cut(s) 134, 625
AfiI CCNNNNNNNGG 1 cut(s) 620
AgsI TTSAA 3 cut(s) 95, 385, 496
AluBI AGCT 8 cut(s) 13, 91, 251, 311, 332, 388, 565, 608
AluI AGCT 8 cut(s) 13, 91, 251, 311, 332, 388, 565, 608
Alw21I GWGCWC 2 cut(s) 297, 417
Alw44I GTGCAC 1 cut(s) 413
AlwNI CAGNNNCTG 1 cut(s) 335
Ama87I CYCGRG 2 cut(s) 446, 578
AoxI GGCC 1 cut(s) 15
ApaLI GTGCAC 1 cut(s) 413
ApoI RAATTY 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 430
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 499
AvaI CYCGRG 2 cut(s) 446, 578
AvaII GGWCC 1 cut(s) 430
AxyI CCTNAGG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 417
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 1 cut(s) 277
Bbv12I GWGCWC 2 cut(s) 297, 417
BbvCI CCTCAGC 1 cut(s) 328
BccI CCATC 3 cut(s) 206, 229, 517
BcgI CGANNNNNNTGC 4 cut(s) 200, 234, 498, 532
BclI TGATCA 2 cut(s) 117, 406
BcnI CCSGG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 306
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 1 cut(s) 430
BmeT110I CYCGRG 2 cut(s) 446, 578
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 299
BmsI GCATC 2 cut(s) 188, 216
Bpu10I CCTNAGC 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 539, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 620
Bse21I CCTNAGG 1 cut(s) 271
Bse3DI GCAATG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 3 cut(s) 118, 179, 198
BseLI CCNNNNNNNGG 1 cut(s) 620
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 3 cut(s) 75, 285, 342
BseSI GKGCMC 1 cut(s) 417
BseYI CCCAGC 1 cut(s) 244
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 277
BsiHKAI GWGCWC 2 cut(s) 297, 417
BsiHKCI CYCGRG 2 cut(s) 446, 578
BsiSI CCGG 2 cut(s) 298, 470
BsiWI CGTACG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 1 cut(s) 620
BsmFI GGGAC 1 cut(s) 427
BsmI GAATGC 2 cut(s) 149, 353
BsnI GGCC 1 cut(s) 17
BsoBI CYCGRG 2 cut(s) 446, 578
Bsp1286I GDGCHC 2 cut(s) 297, 417
Bsp143I GATC 3 cut(s) 117, 406, 574
BspACI CCGC 3 cut(s) 214, 259, 419
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 3 cut(s) 76, 284, 341
BspLI GGNNCC 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 310
BspT107I GGYRCC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 3 cut(s) 117, 406, 574
Bst4CI ACNGT 1 cut(s) 289
BstAPI GCANNNNNTGC 1 cut(s) 421
BstC8I GCNNGC 2 cut(s) 15, 282
BstDEI CTNAG 3 cut(s) 84, 271, 328
BstF5I GGATG 3 cut(s) 118, 179, 198
BstKTI GATC 3 cut(s) 120, 409, 577
BstMBI GATC 3 cut(s) 117, 406, 574
BstMWI GCNNNNNNNGC 5 cut(s) 137, 308, 341, 421, 559
BstSCI CCNGG 1 cut(s) 297
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 417
Bsu36I CCTNAGG 1 cut(s) 271
BsuRI GGCC 1 cut(s) 17
BtsCI GGATG 3 cut(s) 118, 179, 198
BtsIMutI CAGTG 2 cut(s) 81, 294
Cac8I GCNNGC 2 cut(s) 15, 282
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 133, 624
CviAII CATG 1 cut(s) 394
CviQI GTAC 2 cut(s) 133, 624
DdeI CTNAG 3 cut(s) 84, 271, 328
DpnI GATC 3 cut(s) 119, 408, 576
DpnII GATC 3 cut(s) 117, 406, 574
EaeI YGGCCR 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 430
Eco81I CCTNAGG 1 cut(s) 271
Eco88I CYCGRG 2 cut(s) 446, 578
FaeI CATG 1 cut(s) 397
FaiI YATR 8 cut(s) 45, 64, 105, 229, 254, 395, 602, 621
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 1 cut(s) 427
FatI CATG 1 cut(s) 393
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 2 cut(s) 117, 406
FokI GGATG 3 cut(s) 125, 166, 185
GsaI CCCAGC 1 cut(s) 248
HaeIII GGCC 1 cut(s) 17
HapII CCGG 2 cut(s) 298, 470
Hin1II CATG 1 cut(s) 397
HincII GTYRAC 2 cut(s) 456, 543
HindII GTYRAC 2 cut(s) 456, 543
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 377, 581
HpaI GTTAAC 1 cut(s) 456
HpaII CCGG 2 cut(s) 298, 470
HphI GGTGA 1 cut(s) 499
Hpy166II GTNNAC 3 cut(s) 415, 456, 543
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 4 cut(s) 76, 110, 448, 578
Hpy8I GTNNAC 3 cut(s) 415, 456, 543
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 6 cut(s) 221, 308, 351, 371, 415, 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 308, 341, 421, 559
HpyF3I CTNAG 3 cut(s) 84, 271, 328
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 1 cut(s) 397
Ksp22I TGATCA 2 cut(s) 117, 406
KspAI GTTAAC 1 cut(s) 456
Kzo9I GATC 3 cut(s) 117, 406, 574
LmnI GCTCC 3 cut(s) 292, 527, 547
LpnPI CCDG 6 cut(s) 258, 258, 294, 311, 395, 483
LweI GCATC 2 cut(s) 188, 216
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 1 cut(s) 265
MalI GATC 3 cut(s) 119, 408, 576
MbiI CCGCTC 1 cut(s) 214
MboI GATC 3 cut(s) 117, 406, 574
MboII GAAGA 2 cut(s) 117, 532
MhlI GDGCHC 2 cut(s) 297, 417
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 2 cut(s) 358, 616
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 2 cut(s) 371, 590
MnlI CCTC 6 cut(s) 130, 280, 325, 337, 443, 455
Mox20I TGGCCA 1 cut(s) 17
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 168, 455
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 332, 421
MspI CCGG 2 cut(s) 298, 470
MspR9I CCNGG 1 cut(s) 299
Mva1269I GAATGC 2 cut(s) 149, 353
MwoI GCNNNNNNNGC 5 cut(s) 137, 308, 341, 421, 559
NciI CCSGG 1 cut(s) 299
NdeII GATC 3 cut(s) 117, 406, 574
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 578
PctI GAATGC 2 cut(s) 149, 353
Pfl23II CGTACG 1 cut(s) 132
PleI GAGTC 2 cut(s) 371, 589
PpsI GAGTC 2 cut(s) 371, 589
PspFI CCCAGC 1 cut(s) 244
PspLI CGTACG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 335
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 2 cut(s) 134, 625
RsaNI GTAC 2 cut(s) 133, 624
SaqAI TTAA 2 cut(s) 168, 455
Sau3AI GATC 3 cut(s) 117, 406, 574
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 2 cut(s) 371, 590
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 2 cut(s) 297, 417
SfaNI GCATC 2 cut(s) 188, 216
SfcI CTRYAG 1 cut(s) 306
Sfr274I CTCGAG 1 cut(s) 578
SinI GGWCC 1 cut(s) 430
SlaI CTCGAG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 2 cut(s) 358, 616
SsiI CCGC 3 cut(s) 214, 259, 419
StyD4I CCNGG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 443
TaqI TCGA 3 cut(s) 210, 518, 579
TasI AATT 2 cut(s) 358, 616
Tru1I TTAA 2 cut(s) 168, 455
Tru9I TTAA 2 cut(s) 168, 455
TscAI CASTG 2 cut(s) 88, 294
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 1 cut(s) 352
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 2 cut(s) 88, 294
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 1 cut(s) 358
XhoI CTCGAG 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.