pycom111g00780

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0010417
Physical Location & Seq
Forward (+)
538488 .. 540799
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00780.2

Sequence Viewer

Length: 1815 bp
ATGGGTCGGGTGCAGGAGAAGCTGCGGTCCTTCTTCAGCAACCGATGGCTGGTTTTCGTGGCGGCAATGTGGGTGCAGTCCTGTGCGGGAATCGGCTACTTGTTCGGGAGCATATCGCCGGTGATCAAGAGCTCCATGGATTACAATCAGCGGCAGATTTCGCGGCTGGGGGTGGCTAAAGATCTCGGGGACAGCGTTGGGTTTTTGGCGGGGAGTTTGTGCGAGGTGCTGCCTCTGTGGGCGGCGCTGCTCGTTGGCGCTCTGATGAATTTTGTTGGGTACGGTTGGGTGTGGCTTGTTGTTACTGGTCGAGCTCCTTCGTTGAACTTGTGGGCTGTGAGTATCATATCTAACCAAAATGTGCATTCTTATATTCGTGGGAACAAACGGCGAGACCTTCTTCAACACAGTTGCTCTGGTCTCTTGTGTGCAAAACTTCCCAAAAAGTCGGGGTCCCGTGGTTGGAATACTCAAGGGCTTTGCTGGGTTGAGCGGCGCAATTCTGACTCAGATATATGTGATGATCGATCTCTGTCCCCAGACCATGCATCTTTGATTTTCATGGTTGCGGTTGGTCCAGCAATGGTGGTTATTGCATTAATGTTTATAGTCAGACCGGTTGGAGGTCACAGACAAGTCCGGCCAACCGATGCCATGAGCTTCACGTTTATTTATAGCCTGTGCCTCCTATTAGCTGCTTATTTGATGGGGGTCATGCTAGTTGAAGATCTAATTGATTTGAGCCACACCGTGATGGTTATATTTACAGCGGTTTTGTTTGTGCTCCTGTTGATTCCCATTGTGATTCCCATTTCGTTGAGCATTTCCTCCGAGGCAAGATCTCTAGAAGAAGAGGCACTCCTACCCGAGCCACAGACACAAGACCGCGGGAAATCTGGGCACGATGCAAATGAGGTAATATTTAGTGAGGTGGAAGACGAGAAGCCTAAGGAAATGGACTTACTTCCAGCATCCGAAAGGCAAAAACGAATTGCTCAATTGCAATCGAAACTATTCCAAGCAGCTGCCGATGGAGCAGTTAGGGTTAAGAGGAGGAGAGGTCCACATAGAGGGGAGGACTTCACATTGACACAAGCTTTGATCAAAGCTGACTTTTGGCTTATGTTTTTCTCACTTCTCTTGGGTTCTGGATCTGGTTTAACGGTGATTGATAACCTCGGTCAGATGAGCCAGTCTCTCGGGTATGACAATACGCATATATTTGTATCCCTGATCAGCATCTGGAACTTTCTTGGTCGGGTTGGTGGCGGTTACTTCTCTGAGATTATTGTGAGGGACTTTGCTTATCCAAGACCGGTAGCAATGGCTGTGGCACAACTCCTTATGGCAGTTGGGCATATCTTCATTGCTTTCGGATGGCCTGGGGCAATGCACATTGCCACCATCTTGGTTGGACTAGGCTACGGGGCTCATTGGGCGATTGTGCCAGCTGCTGCTTCTGAATTGTTTGGCTTGAAAAAGTTTGGGGCTTTATACAATTTCCTCACGATTGCAAACCCTGCTGGTTCGTTGCTCTTTTCTGGTGTGATTGCTAGCAGTATATACGACTTTGAAGCTGAGAAGCAAGCTCATCAGCACCATCACCCGCTTATGAGTCCAGGAACATCAGTTTTCCAAGGCATGCTTCGAATGGACGCACCAAAGTGTGACGGTTCTATATGCTTCTTCTTAACTTCGTTAATCATGTCCGGATTATGCATTGTTGCCTTTGTTTTAAGCATGATTCTGGTACATCGGACTAAGATTGTATATGCCCACCTGTATGGAAAATCACGTCCAGCTGGGCTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

605

Amino Acids

66.28

Weight (kDa)

8.71

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010417)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01930 AT3G01930 AT5G14120
fragaria_vesca FvH4_6g53700 FvH4_6g53700 FvH4_6g53700
malus_domestica MD09G1009800.v1.1 MD17G1000700.v1.1
prunus_persica Prupe.3G307300_v2.0.a1
pyrus_communis pycom111g00780 pycom17g00820
rosa_chinensis RchiOBHm_Chr2g0175771
rosa_laevigata RLG00000022357
rosa_multiflora Rmu_sc0016745.1_g000001
rosa_roxburghii Rroxscaffold_2G00077030
rosa_rugosa Rorug02G0587600
rosa_samantha Rh2AG666600 Rh2BG678000 Rh2CG641100 Rh2DG691800
rosa_wichuraiana Rw2G054660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 493
AccII CGCG 2 cut(s) 163, 888
AccIII TCCGGA 1 cut(s) 1709
AclWI GGATC 1 cut(s) 1159
AcoI YGGCCR 1 cut(s) 641
AcsI RAATTY 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 19
AdeI CACNNNGTG 1 cut(s) 751
AfaI GTAC 2 cut(s) 281, 1752
AfiI CCNNNNNNNGG 4 cut(s) 49, 462, 623, 1070
AgeI ACCGGT 2 cut(s) 616, 1315
AgsI TTSAA 5 cut(s) 325, 404, 725, 1477, 1574
AjiI CACGTC 1 cut(s) 1796
AjnI CCWGG 2 cut(s) 1381, 1618
AjuI GAANNNNNNNTTGG 2 cut(s) 1629, 1661
AleI CACNNNNGTG 1 cut(s) 1663
Alw21I GWGCWC 3 cut(s) 134, 316, 786
Alw26I GTCTC 3 cut(s) 387, 425, 1200
AlwI GGATC 1 cut(s) 1159
AlwNI CAGNNNCTG 2 cut(s) 1242, 1454
Ama87I CYCGRG 3 cut(s) 185, 866, 1199
Aor13HI TCCGGA 1 cut(s) 1709
AoxI GGCC 2 cut(s) 641, 1379
ApeKI GCWGC 8 cut(s) 22, 229, 247, 695, 1022, 1025, 1451, 1454
ApoI RAATTY 1 cut(s) 268
ArsI GACNNNNNNTTYG 2 cut(s) 1081, 1113
AseI ATTAAT 1 cut(s) 599
AsiGI ACCGGT 2 cut(s) 616, 1315
Asp700I GAANNNNTTC 1 cut(s) 1013
AspLEI GCGC 3 cut(s) 247, 260, 498
AspS9I GGNCC 4 cut(s) 27, 453, 575, 1061
AsuHPI GGTGA 3 cut(s) 133, 1177, 1595
AsuII TTCGAA 1 cut(s) 1648
AsuNHI GCTAGC 1 cut(s) 1553
AvaI CYCGRG 3 cut(s) 185, 866, 1199
AvaII GGWCC 4 cut(s) 27, 453, 575, 1061
AxyI CCTNAGG 1 cut(s) 948
BaeGI GKGCMC 1 cut(s) 903
BanII GRGCYC 3 cut(s) 134, 316, 1432
BbsI GAAGAC 1 cut(s) 942
Bbv12I GWGCWC 3 cut(s) 134, 316, 786
BbvI GCAGC 8 cut(s) 9, 216, 234, 682, 1012, 1034, 1438, 1441
BccI CCATC 7 cut(s) 39, 700, 748, 1025, 1371, 1412, 1608
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 1383, 1620
BciVI GTATCC 1 cut(s) 1237
BclI TGATCA 3 cut(s) 123, 1101, 1233
BcoDI GTCTC 3 cut(s) 387, 425, 1200
BfaI CTAG 4 cut(s) 719, 845, 1418, 1554
BfoI RGCGCY 2 cut(s) 248, 261
BfuI GTATCC 1 cut(s) 1237
BglII AGATCT 3 cut(s) 181, 727, 839
Bme1390I CCNGG 2 cut(s) 1383, 1620
Bme18I GGWCC 4 cut(s) 27, 453, 575, 1061
BmeT110I CYCGRG 3 cut(s) 185, 866, 1199
BmgBI CACGTC 1 cut(s) 1796
BmgT120I GGNCC 4 cut(s) 27, 453, 575, 1061
BmiI GGNNCC 2 cut(s) 454, 455
BmrFI CCNGG 2 cut(s) 1383, 1620
BmsI GCATC 5 cut(s) 557, 640, 895, 980, 1248
BmtI GCTAGC 1 cut(s) 1557
BpiI GAAGAC 1 cut(s) 942
BplI GAGNNNNNCTC 2 cut(s) 1180, 1212
Bpu14I TTCGAA 1 cut(s) 1648
BpuEI CTTGAG 1 cut(s) 456
Bsa29I ATCGAT 1 cut(s) 526
BsaBI GATNNNNATC 2 cut(s) 144, 1238
BsaI GGTCTC 2 cut(s) 387, 425
BsaJI CCNNGG 7 cut(s) 135, 457, 831, 886, 1177, 1382, 1636
BsaWI WCCGGW 3 cut(s) 616, 1315, 1709
BsaXI ACNNNNNCTCC 2 cut(s) 1045, 1075
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 462, 623, 1070
Bse118I RCCGGY 3 cut(s) 118, 616, 1315
Bse1I ACTGG 2 cut(s) 310, 1192
Bse21I CCTNAGG 1 cut(s) 948
Bse3DI GCAATG 6 cut(s) 72, 588, 1329, 1365, 1395, 1395
Bse8I GATNNNNATC 2 cut(s) 144, 1238
BseAI TCCGGA 1 cut(s) 1709
BseBI CCWGG 2 cut(s) 1383, 1620
BseCI ATCGAT 1 cut(s) 526
BseDI CCNNGG 7 cut(s) 135, 457, 831, 886, 1177, 1382, 1636
BseGI GGATG 2 cut(s) 971, 1382
BseJI GATNNNNATC 2 cut(s) 144, 1238
BseLI CCNNNNNNNGG 4 cut(s) 49, 462, 623, 1070
BseMI GCAATG 6 cut(s) 72, 588, 1329, 1365, 1395, 1395
BseMII CTCAG 3 cut(s) 522, 1272, 1569
BseNI ACTGG 2 cut(s) 310, 1192
BseRI GAGGAG 2 cut(s) 1066, 1069
BseSI GKGCMC 1 cut(s) 903
BseXI GCAGC 8 cut(s) 9, 216, 234, 682, 1012, 1034, 1438, 1441
BseYI CCCAGC 3 cut(s) 166, 483, 1802
BsgI GTGCAG 2 cut(s) 32, 95
Bsh1236I CGCG 2 cut(s) 163, 888
BshFI GGCC 2 cut(s) 643, 1381
BshTI ACCGGT 2 cut(s) 616, 1315
BshVI ATCGAT 1 cut(s) 526
BsiHKAI GWGCWC 3 cut(s) 134, 316, 786
BsiHKCI CYCGRG 3 cut(s) 185, 866, 1199
BsiSI CCGG 5 cut(s) 119, 617, 640, 1316, 1710
BslFI GGGAC 4 cut(s) 203, 439, 520, 1310
BslI CCNNNNNNNGG 4 cut(s) 49, 462, 623, 1070
BsmAI GTCTC 3 cut(s) 387, 425, 1200
BsmFI GGGAC 4 cut(s) 203, 439, 520, 1310
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 2 cut(s) 643, 1381
Bso31I GGTCTC 2 cut(s) 387, 425
BsoBI CYCGRG 3 cut(s) 185, 866, 1199
Bsp119I TTCGAA 1 cut(s) 1648
Bsp1286I GDGCHC 5 cut(s) 134, 316, 786, 903, 1432
Bsp13I TCCGGA 1 cut(s) 1709
Bsp143I GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
Bsp19I CCATGG 1 cut(s) 135
BspANI GGCC 2 cut(s) 643, 1381
BspCNI CTCAG 3 cut(s) 521, 1273, 1570
BspDI ATCGAT 1 cut(s) 526
BspEI TCCGGA 1 cut(s) 1709
BspFNI CGCG 2 cut(s) 163, 888
BspHI TCATGA 1 cut(s) 1811
BspLI GGNNCC 2 cut(s) 454, 455
BspOI GCTAGC 1 cut(s) 1557
BspPI GGATC 1 cut(s) 1159
BspT104I TTCGAA 1 cut(s) 1648
BspTNI GGTCTC 2 cut(s) 387, 425
BsrBI CCGCTC 1 cut(s) 493
BsrDI GCAATG 6 cut(s) 72, 588, 1329, 1365, 1395, 1395
BsrFI RCCGGY 3 cut(s) 118, 616, 1315
BsrI ACTGG 2 cut(s) 310, 1192
BssAI RCCGGY 3 cut(s) 118, 616, 1315
BssECI CCNNGG 7 cut(s) 135, 457, 831, 886, 1177, 1382, 1636
BssMI GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
BssT1I CCWWGG 2 cut(s) 135, 1636
Bst2UI CCWGG 2 cut(s) 1383, 1620
Bst4CI ACNGT 5 cut(s) 284, 410, 751, 1165, 1673
Bst6I CTCTTC 1 cut(s) 846
BstAPI GCANNNNNTGC 1 cut(s) 1520
BstBI TTCGAA 1 cut(s) 1648
BstC8I GCNNGC 4 cut(s) 1449, 1555, 1587, 1643
BstDEI CTNAG 5 cut(s) 508, 948, 1281, 1578, 1761
BstDSI CCRYGG 3 cut(s) 135, 457, 886
BstF5I GGATG 2 cut(s) 971, 1382
BstFNI CGCG 2 cut(s) 163, 888
BstH2I RGCGCY 2 cut(s) 248, 261
BstHHI GCGC 3 cut(s) 247, 260, 498
BstKTI GATC 9 cut(s) 126, 184, 526, 530, 730, 842, 1104, 1154, 1236
BstMAI GTCTC 3 cut(s) 387, 425, 1200
BstMBI GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
BstMWI GCNNNNNNNGC 5 cut(s) 19, 160, 1034, 1436, 1520
BstNI CCWGG 2 cut(s) 1383, 1620
BstNSI RCATGY 1 cut(s) 1645
BstSCI CCNGG 2 cut(s) 1381, 1618
BstSLI GKGCMC 1 cut(s) 903
BstUI CGCG 2 cut(s) 163, 888
BstV1I GCAGC 8 cut(s) 9, 216, 234, 682, 1012, 1034, 1438, 1441
BstV2I GAAGAC 1 cut(s) 942
BstX2I RGATCY 4 cut(s) 181, 727, 839, 1151
BstXI CCANNNNNNTGG 2 cut(s) 1408, 1784
BstYI RGATCY 4 cut(s) 181, 727, 839, 1151
Bsu15I ATCGAT 1 cut(s) 526
Bsu36I CCTNAGG 1 cut(s) 948
BsuI GTATCC 1 cut(s) 1237
BsuRI GGCC 2 cut(s) 643, 1381
BsuTUI ATCGAT 1 cut(s) 526
BtgI CCRYGG 3 cut(s) 135, 457, 886
BtrI CACGTC 1 cut(s) 1796
BtsCI GGATG 2 cut(s) 971, 1382
Cac8I GCNNGC 4 cut(s) 1449, 1555, 1587, 1643
CaiI CAGNNNCTG 2 cut(s) 1242, 1454
CciI TCATGA 1 cut(s) 1811
CfoI GCGC 3 cut(s) 247, 260, 498
Cfr10I RCCGGY 3 cut(s) 118, 616, 1315
Cfr13I GGNCC 4 cut(s) 27, 453, 575, 1061
Cfr42I CCGCGG 1 cut(s) 889
ClaI ATCGAT 1 cut(s) 526
CseI GACGC 1 cut(s) 1664
Csp6I GTAC 2 cut(s) 280, 1751
CspAI ACCGGT 2 cut(s) 616, 1315
CspCI CAANNNNNGTGG 2 cut(s) 1311, 1346
CviAII CATG 9 cut(s) 136, 545, 562, 655, 715, 1642, 1705, 1741, 1812
CviQI GTAC 2 cut(s) 280, 1751
DdeI CTNAG 5 cut(s) 508, 948, 1281, 1578, 1761
DpnI GATC 9 cut(s) 125, 183, 525, 529, 729, 841, 1103, 1153, 1235
DpnII GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
DraIII CACNNNGTG 1 cut(s) 751
EaeI YGGCCR 1 cut(s) 641
Eam1104I CTCTTC 1 cut(s) 846
EarI CTCTTC 1 cut(s) 846
Ecl136II GAGCTC 2 cut(s) 132, 314
Eco130I CCWWGG 2 cut(s) 135, 1636
Eco24I GRGCYC 3 cut(s) 134, 316, 1432
Eco31I GGTCTC 2 cut(s) 387, 425
Eco47I GGWCC 4 cut(s) 27, 453, 575, 1061
Eco53kI GAGCTC 2 cut(s) 132, 314
Eco57I CTGAAG 1 cut(s) 19
Eco81I CCTNAGG 1 cut(s) 948
Eco88I CYCGRG 3 cut(s) 185, 866, 1199
EcoICRI GAGCTC 2 cut(s) 132, 314
EcoO109I RGGNCCY 1 cut(s) 453
EcoRII CCWGG 2 cut(s) 1381, 1618
EcoT14I CCWWGG 2 cut(s) 135, 1636
EcoT22I ATGCAT 2 cut(s) 550, 1721
EcoT38I GRGCYC 3 cut(s) 134, 316, 1432
ErhI CCWWGG 2 cut(s) 135, 1636
FaeI CATG 9 cut(s) 139, 548, 565, 658, 718, 1645, 1708, 1744, 1815
FalI AAGNNNNNCTT 2 cut(s) 1629, 1661
FaqI GGGAC 4 cut(s) 203, 439, 520, 1310
FatI CATG 9 cut(s) 135, 544, 561, 654, 714, 1641, 1704, 1740, 1811
FauI CCCGC 4 cut(s) 79, 202, 881, 1614
FbaI TGATCA 3 cut(s) 123, 1101, 1233
FokI GGATG 2 cut(s) 958, 1389
FriOI GRGCYC 3 cut(s) 134, 316, 1432
FspBI CTAG 4 cut(s) 719, 845, 1418, 1554
GlaI GCGC 3 cut(s) 246, 259, 497
GsaI CCCAGC 3 cut(s) 170, 487, 1806
HaeII RGCGCY 2 cut(s) 248, 261
HaeIII GGCC 2 cut(s) 643, 1381
HapII CCGG 5 cut(s) 119, 617, 640, 1316, 1710
HgaI GACGC 1 cut(s) 1664
HhaI GCGC 3 cut(s) 247, 260, 498
Hin1II CATG 9 cut(s) 139, 548, 565, 658, 718, 1645, 1708, 1744, 1815
Hin6I GCGC 3 cut(s) 245, 258, 496
HinP1I GCGC 3 cut(s) 245, 258, 496
HindIII AAGCTT 1 cut(s) 1095
HinfI GANTC 6 cut(s) 90, 506, 793, 805, 1615, 1744
HpaII CCGG 5 cut(s) 119, 617, 640, 1316, 1710
HphI GGTGA 3 cut(s) 133, 1177, 1595
Hpy166II GTNNAC 1 cut(s) 1064
Hpy188III TCNNGA 8 cut(s) 106, 127, 845, 1149, 1243, 1507, 1710, 1812
Hpy8I GTNNAC 1 cut(s) 1064
HpyAV CCTTC 3 cut(s) 40, 327, 407
HpyCH4III ACNGT 5 cut(s) 284, 410, 751, 1165, 1673
HpyCH4IV ACGT 2 cut(s) 665, 1795
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 160, 1034, 1436, 1520
HpyF3I CTNAG 5 cut(s) 508, 948, 1281, 1578, 1761
HpySE526I ACGT 2 cut(s) 665, 1795
Hsp92II CATG 9 cut(s) 139, 548, 565, 658, 718, 1645, 1708, 1744, 1815
HspAI GCGC 3 cut(s) 245, 258, 496
KflI GGGWCCC 1 cut(s) 453
Kpn2I TCCGGA 1 cut(s) 1709
Ksp22I TGATCA 3 cut(s) 123, 1101, 1233
KspI CCGCGG 1 cut(s) 889
Kzo9I GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
LmnI GCTCC 5 cut(s) 108, 137, 319, 789, 1034
Lsp1109I GCAGC 8 cut(s) 9, 216, 234, 682, 1012, 1034, 1438, 1441
LweI GCATC 5 cut(s) 557, 640, 895, 980, 1248
MaeI CTAG 4 cut(s) 719, 845, 1418, 1554
MaeII ACGT 2 cut(s) 665, 1795
MaeIII GTNAC 4 cut(s) 301, 626, 1271, 1667
MalI GATC 9 cut(s) 125, 183, 525, 529, 729, 841, 1103, 1153, 1235
MbiI CCGCTC 1 cut(s) 493
MboI GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
MboII GAAGA 8 cut(s) 25, 392, 737, 860, 863, 947, 1354, 1678
MfeI CAATTG 1 cut(s) 998
MflI RGATCY 4 cut(s) 181, 727, 839, 1151
MhlI GDGCHC 5 cut(s) 134, 316, 786, 903, 1432
MluCI AATT 7 cut(s) 268, 499, 732, 990, 998, 1463, 1498
MlyI GAGTC 2 cut(s) 500, 1624
MmeI TCCRAC 3 cut(s) 443, 601, 1393
Mph1103I ATGCAT 2 cut(s) 550, 1721
MroI TCCGGA 1 cut(s) 1709
MroXI GAANNNNTTC 1 cut(s) 1013
MseI TTAA 6 cut(s) 599, 1047, 1160, 1691, 1700, 1736
MslI CAYNNNNRTG 3 cut(s) 752, 1663, 1782
MspA1I CMGCKG 6 cut(s) 151, 770, 888, 1025, 1451, 1802
MspI CCGG 5 cut(s) 119, 617, 640, 1316, 1710
MspR9I CCNGG 2 cut(s) 1383, 1620
MunI CAATTG 1 cut(s) 998
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 2 cut(s) 1383, 1620
MvnI CGCG 2 cut(s) 163, 888
MwoI GCNNNNNNNGC 5 cut(s) 19, 160, 1034, 1436, 1520
NcoI CCATGG 1 cut(s) 135
NdeII GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
NheI GCTAGC 1 cut(s) 1553
NlaIII CATG 9 cut(s) 139, 548, 565, 658, 718, 1645, 1708, 1744, 1815
NlaIV GGNNCC 2 cut(s) 454, 455
NmuCI GTSAC 2 cut(s) 626, 1667
NsiI ATGCAT 2 cut(s) 550, 1721
NspI RCATGY 1 cut(s) 1645
NspV TTCGAA 1 cut(s) 1648
OliI CACNNNNGTG 1 cut(s) 1663
PaeI GCATGC 1 cut(s) 1645
PagI TCATGA 1 cut(s) 1811
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 1013
PfeI GAWTC 4 cut(s) 90, 793, 805, 1744
PfoI TCCNGGA 1 cut(s) 1618
PinAI ACCGGT 2 cut(s) 616, 1315
PleI GAGTC 2 cut(s) 500, 1623
PpsI GAGTC 2 cut(s) 500, 1623
PpuMI RGGWCCY 1 cut(s) 453
PshBI ATTAAT 1 cut(s) 599
Psp124BI GAGCTC 2 cut(s) 134, 316
Psp5II RGGWCCY 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 1381, 1618
PspFI CCCAGC 3 cut(s) 166, 483, 1802
PspGI CCWGG 2 cut(s) 1381, 1618
PspN4I GGNNCC 2 cut(s) 454, 455
PspPI GGNCC 4 cut(s) 27, 453, 575, 1061
PspPPI RGGWCCY 1 cut(s) 453
PstNI CAGNNNCTG 2 cut(s) 1242, 1454
PsuI RGATCY 4 cut(s) 181, 727, 839, 1151
PvuII CAGCTG 3 cut(s) 1025, 1451, 1802
RsaI GTAC 2 cut(s) 281, 1752
RsaNI GTAC 2 cut(s) 280, 1751
RseI CAYNNNNRTG 3 cut(s) 752, 1663, 1782
SacI GAGCTC 2 cut(s) 134, 316
SacII CCGCGG 1 cut(s) 889
SaqAI TTAA 6 cut(s) 599, 1047, 1160, 1691, 1700, 1736
Sau3AI GATC 9 cut(s) 123, 181, 523, 527, 727, 839, 1101, 1151, 1233
Sau96I GGNCC 4 cut(s) 27, 453, 575, 1061
SchI GAGTC 2 cut(s) 500, 1624
ScrFI CCNGG 2 cut(s) 1383, 1620
SduI GDGCHC 5 cut(s) 134, 316, 786, 903, 1432
SfaNI GCATC 5 cut(s) 557, 640, 895, 980, 1248
Sfr303I CCGCGG 1 cut(s) 889
SfuI TTCGAA 1 cut(s) 1648
SgrAI CRCCGGYG 1 cut(s) 118
SgrBI CCGCGG 1 cut(s) 889
SinI GGWCC 4 cut(s) 27, 453, 575, 1061
SmiMI CAYNNNNRTG 3 cut(s) 752, 1663, 1782
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
SphI GCATGC 1 cut(s) 1645
Sse9I AATT 7 cut(s) 268, 499, 732, 990, 998, 1463, 1498
SspI AATATT 1 cut(s) 921
SspMI CTAG 4 cut(s) 719, 845, 1418, 1554
SstI GAGCTC 2 cut(s) 134, 316
StyD4I CCNGG 2 cut(s) 1381, 1618
StyI CCWWGG 2 cut(s) 135, 1636
TaaI ACNGT 5 cut(s) 284, 410, 751, 1165, 1673
TaiI ACGT 2 cut(s) 668, 1798
TaqI TCGA 4 cut(s) 310, 526, 1007, 1648
TaqII GACCGA 1 cut(s) 1169
TasI AATT 7 cut(s) 268, 499, 732, 990, 998, 1463, 1498
TauI GCSGC 5 cut(s) 65, 154, 166, 245, 496
TfiI GAWTC 4 cut(s) 90, 793, 805, 1744
Tru1I TTAA 6 cut(s) 599, 1047, 1160, 1691, 1700, 1736
Tru9I TTAA 6 cut(s) 599, 1047, 1160, 1691, 1700, 1736
TseFI GTSAC 2 cut(s) 626, 1667
TseI GCWGC 8 cut(s) 22, 229, 247, 695, 1022, 1025, 1451, 1454
Tsp45I GTSAC 2 cut(s) 626, 1667
TspDTI ATGAA 4 cut(s) 281, 550, 1354, 1800
VpaK11BI GGWCC 4 cut(s) 27, 453, 575, 1061
VspI ATTAAT 1 cut(s) 599
XapI RAATTY 1 cut(s) 268
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 1 cut(s) 1645
XmnI GAANNNNTTC 1 cut(s) 1013
XspI CTAG 4 cut(s) 719, 845, 1418, 1554
Zsp2I ATGCAT 2 cut(s) 550, 1721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.