pycom111g02460

monodehydroascorbate reductase, cytoplasmic isoform 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0011705
Physical Location & Seq
Forward (+)
1465975 .. 1468891
2917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1230 bp
ATGGGAAGGGCTTTTGTTCATGTTATTCTGGGAGGAGGGGTGGCTGCTGGGTATGCAGCTCTTGAATTCAACAAGAGAGGCATCTCCCATGGTGAGCTCTGCATCATTTCTGAAGAATCAGTTCCACCTTATGAGAGACCTGCTTTAAGCAAAGGCTTTCTACTTCCAGAAGCGCCTGCACGCCTTCCATCATTTCATACTTGTGTCGGTGCTAAGGAGGAAAGGTTAACTCCAAAATGGTATAAGGAGCATGGGATCGAATTAGTTATAGGAACTCGAGTCAAGTCTGTTGATGTGAGACGCAAGACACTACTGACAGAATCTGGAGAGACTATAAGTTACAAAGTTCTCATCATTGCAACAGGTGCTCGGGCACTGAAGCTGGAAGAATTTGGAGTCAAAGGATCAGATTCTGAAAATGTGTGTTATTTACAAGATTTGGCTGATGCCAATAGACTAGTAAACTTGATGGAATCTTCCTCTGGTGGGAATGCTATTGTCATTGGTGGTGGCTACATTGGAATGGAGTGTGCTGCATCATTAGCGATCAATAGGATGAATGTAACGATGGTTTTTCCAGAAGAACATTGCATGGCCCGATTATTCACACCCAAGATCGCAAGTTTTTATGAAGAATTTTACAAATCCAAAGGAGTGAAGTTTGTTAAAGGAACCATTTTGTCCTCGTTTGACATTGACTCGGACGGGAAGGTGACTGCTGTTAATCTTAGAGATGGAAGTAGTCTTCCTGCAGACGTGGTTGTTGTGGGAATAGGAATTCGTCCAAACACGAGCCTGTTTGAAGGTCAACTAAAGCTGGAGAAAGGTGGGATCAAAGTGAATGGAAAGATGCTGTCAAGTAATAGCTCAGTCTATGCAGTGGGAGATATTGCCACATTTCCAGTAAAAGTATTTGGTGAGTCCAGAAGGCTCGAACACGTTGACTCAGCAAGAAAATCCGCAAGACATGTTGTTACTGCAATTATGGAACGAAACCAAGTTGATGAATTCGACTATCTACCATTCTTTTACTCGAGAATCTTCACCCTGTCTTGGCAGTTTTACGGGGACAATGCAGGAGATGTAGTCCATTTCGGAGACTTCTCAGGAGGCACATTCGGGGCTTATTGGGTAAGAAAAGGTCAACTTGTCGGGTCTTTTCTCGAAGGCGGAACCAAGGAAGAGTATGAAGCTATAGCCAAGGCCACCAAGCTGAAGCCAGAAAGTTGA

Protein Analysis

410

Amino Acids

44.62

Weight (kDa)

7.09

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 7 - 321 1.2e-54 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 86 - 288 2.5e-06 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 165 - 245 1.5e-13 Pyridine nucleotide-disulphide oxidoreductase
MDHAR3-like_C PF21791 340 - 408 4.9e-32 Monodehydroascorbate reductase 3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011705)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27820
fragaria_vesca FvH4_6g50920 FvH4_6g50920
malus_domestica MD09G1030800.v1.1 MD17G1032700.v1.1
prunus_persica Prupe.3G286700_v2.0.a1
pyrus_communis pycom111g02460 pycom17g03010 pycom17g03020
rosa_chinensis RchiOBHm_Chr2g0172301
rosa_laevigata RLG00000022103
rosa_multiflora Rmu_sc0000467.1_g000013
rosa_roxburghii Rroxscaffold_2G00080000
rosa_rugosa Rorug02G0558600
rosa_samantha Rh2AG633600 Rh2BG647200 Rh2CG614100 Rh2DG662000
rosa_wichuraiana Rw2G052460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 148
AciI CCGC 2 cut(s) 960, 1170
AclWI GGATC 3 cut(s) 263, 412, 839
AcsI RAATTY 5 cut(s) 65, 389, 635, 777, 1007
AcuI CTGAAG 2 cut(s) 132, 398
AfiI CCNNNNNNNGG 2 cut(s) 486, 1053
AflIII ACRYGT 2 cut(s) 937, 967
AgsI TTSAA 3 cut(s) 65, 70, 803
AhlI ACTAGT 1 cut(s) 457
AjiI CACGTC 1 cut(s) 757
AluBI AGCT 7 cut(s) 59, 97, 382, 817, 867, 1193, 1213
AluI AGCT 7 cut(s) 59, 97, 382, 817, 867, 1193, 1213
Alw21I GWGCWC 2 cut(s) 99, 370
Alw26I GTCTC 4 cut(s) 130, 292, 323, 1092
AlwI GGATC 3 cut(s) 263, 412, 839
AlwNI CAGNNNCTG 2 cut(s) 323, 413
Ama87I CYCGRG 3 cut(s) 276, 369, 1033
AoxI GGCC 2 cut(s) 594, 1203
ApeKI GCWGC 3 cut(s) 44, 56, 533
ApoI RAATTY 5 cut(s) 65, 389, 635, 777, 1007
Asp700I GAANNNNTTC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 595
AsuHPI GGTGA 4 cut(s) 104, 724, 929, 1036
AvaI CYCGRG 3 cut(s) 276, 369, 1033
BaeGI GKGCMC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 790
BbsI GAAGAC 1 cut(s) 737
Bbv12I GWGCWC 2 cut(s) 99, 370
BbvI GCAGC 3 cut(s) 31, 68, 520
BccI CCATC 4 cut(s) 196, 463, 562, 728
BcoDI GTCTC 4 cut(s) 130, 292, 323, 1092
BcuI ACTAGT 1 cut(s) 457
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 2 cut(s) 750, 1194
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 148
BisI GCNGC 3 cut(s) 45, 57, 534
BlsI GCNGC 3 cut(s) 46, 58, 535
BmeT110I CYCGRG 3 cut(s) 276, 369, 1033
BmgBI CACGTC 1 cut(s) 757
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 2 cut(s) 673, 1174
BmsI GCATC 5 cut(s) 90, 111, 436, 545, 840
BpiI GAAGAC 1 cut(s) 737
BpmI CTGGAG 2 cut(s) 345, 839
Bpu10I CCTNAGC 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 88, 1176, 1200
BsaXI ACNNNNNCTCC 6 cut(s) 24, 54, 209, 239, 1071, 1101
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 1053
Bse1I ACTGG 1 cut(s) 902
Bse3DI GCAATG 2 cut(s) 354, 586
BseDI CCNNGG 3 cut(s) 88, 1176, 1200
BseGI GGATG 1 cut(s) 561
BseLI CCNNNNNNNGG 2 cut(s) 486, 1053
BseMI GCAATG 2 cut(s) 354, 586
BseMII CTCAG 3 cut(s) 882, 960, 1119
BseNI ACTGG 1 cut(s) 902
BseRI GAGGAG 1 cut(s) 48
BseSI GKGCMC 1 cut(s) 376
BseXI GCAGC 3 cut(s) 31, 68, 520
BseYI CCCAGC 1 cut(s) 47
BsgI GTGCAG 1 cut(s) 162
BshFI GGCC 2 cut(s) 596, 1205
BsiHKAI GWGCWC 2 cut(s) 99, 370
BsiHKCI CYCGRG 3 cut(s) 276, 369, 1033
BslFI GGGAC 1 cut(s) 1082
BslI CCNNNNNNNGG 2 cut(s) 486, 1053
BsmAI GTCTC 4 cut(s) 130, 292, 323, 1092
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 1 cut(s) 1082
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 2 cut(s) 596, 1205
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 3 cut(s) 276, 369, 1033
Bsp1286I GDGCHC 3 cut(s) 99, 370, 376
Bsp143I GATC 5 cut(s) 255, 404, 546, 615, 831
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 2 cut(s) 960, 1170
BspANI GGCC 2 cut(s) 596, 1205
BspCNI CTCAG 3 cut(s) 881, 959, 1118
BspLI GGNNCC 2 cut(s) 673, 1174
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 148
BspPI GGATC 3 cut(s) 263, 412, 839
BspTNI GGTCTC 1 cut(s) 130
BsrDI GCAATG 2 cut(s) 354, 586
BsrI ACTGG 1 cut(s) 902
BssECI CCNNGG 3 cut(s) 88, 1176, 1200
BssMI GATC 5 cut(s) 255, 404, 546, 615, 831
BssSI CACGAG 1 cut(s) 790
BssT1I CCWWGG 3 cut(s) 88, 1176, 1200
Bst2BI CACGAG 1 cut(s) 790
Bst6I CTCTTC 1 cut(s) 1176
BstAPI GCANNNNNTGC 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 177, 181
BstDEI CTNAG 5 cut(s) 213, 728, 868, 946, 1105
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 1 cut(s) 561
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 5 cut(s) 258, 407, 549, 618, 834
BstMAI GTCTC 4 cut(s) 130, 292, 323, 1092
BstMBI GATC 5 cut(s) 255, 404, 546, 615, 831
BstMWI GCNNNNNNNGC 3 cut(s) 53, 365, 542
BstNSI RCATGY 1 cut(s) 971
BstSFI CTRYAG 2 cut(s) 750, 1194
BstSLI GKGCMC 1 cut(s) 376
BstV1I GCAGC 3 cut(s) 31, 68, 520
BstV2I GAAGAC 1 cut(s) 737
BsuRI GGCC 2 cut(s) 596, 1205
BtgI CCRYGG 1 cut(s) 88
BtrI CACGTC 1 cut(s) 757
BtsCI GGATG 1 cut(s) 561
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 2 cut(s) 374, 885
BveI ACCTGC 1 cut(s) 148
Cac8I GCNNGC 2 cut(s) 177, 181
CaiI CAGNNNCTG 2 cut(s) 323, 413
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 595
CseI GACGC 1 cut(s) 309
CviAII CATG 5 cut(s) 20, 89, 251, 592, 968
DdeI CTNAG 5 cut(s) 213, 728, 868, 946, 1105
DpnI GATC 5 cut(s) 257, 406, 548, 617, 833
DpnII GATC 5 cut(s) 255, 404, 546, 615, 831
Eam1104I CTCTTC 1 cut(s) 1176
EarI CTCTTC 1 cut(s) 1176
EciI GGCGGA 1 cut(s) 1185
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 3 cut(s) 88, 1176, 1200
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 132, 398
Eco88I CYCGRG 3 cut(s) 276, 369, 1033
EcoICRI GAGCTC 1 cut(s) 97
EcoRI GAATTC 3 cut(s) 65, 777, 1007
EcoT14I CCWWGG 3 cut(s) 88, 1176, 1200
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 3 cut(s) 88, 1176, 1200
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 5 cut(s) 23, 92, 254, 595, 971
FalI AAGNNNNNCTT 2 cut(s) 1131, 1163
FaqI GGGAC 1 cut(s) 1082
FatI CATG 5 cut(s) 19, 88, 250, 591, 967
Fnu4HI GCNGC 3 cut(s) 45, 57, 534
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 3 cut(s) 45, 57, 534
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 174
GluI GCNGC 3 cut(s) 45, 57, 534
GsaI CCCAGC 1 cut(s) 51
GsuI CTGGAG 2 cut(s) 345, 839
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 2 cut(s) 596, 1205
HgaI GACGC 1 cut(s) 309
HhaI GCGC 1 cut(s) 175
Hin1II CATG 5 cut(s) 23, 92, 254, 595, 971
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HincII GTYRAC 4 cut(s) 228, 809, 943, 1145
HindII GTYRAC 4 cut(s) 228, 809, 943, 1145
HpaI GTTAAC 1 cut(s) 228
HphI GGTGA 4 cut(s) 104, 724, 929, 1036
Hpy166II GTNNAC 5 cut(s) 228, 463, 809, 943, 1145
Hpy188I TCNGA 5 cut(s) 112, 409, 415, 703, 1097
Hpy188III TCNNGA 8 cut(s) 62, 167, 324, 578, 924, 1035, 1107, 1163
Hpy8I GTNNAC 5 cut(s) 228, 463, 809, 943, 1145
HpyAV CCTTC 5 cut(s) 194, 703, 797, 921, 1160
HpyCH4IV ACGT 2 cut(s) 756, 939
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 365, 542
HpyF3I CTNAG 5 cut(s) 213, 728, 868, 946, 1105
HpySE526I ACGT 2 cut(s) 756, 939
Hsp92II CATG 5 cut(s) 23, 92, 254, 595, 971
HspAI GCGC 1 cut(s) 173
KspAI GTTAAC 1 cut(s) 228
Kzo9I GATC 5 cut(s) 255, 404, 546, 615, 831
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 3 cut(s) 31, 68, 520
LweI GCATC 5 cut(s) 90, 111, 436, 545, 840
MaeI CTAG 1 cut(s) 458
MaeII ACGT 2 cut(s) 756, 939
MaeIII GTNAC 4 cut(s) 338, 562, 712, 973
MalI GATC 5 cut(s) 257, 406, 548, 617, 833
MboI GATC 5 cut(s) 255, 404, 546, 615, 831
MboII GAAGA 8 cut(s) 125, 398, 468, 593, 644, 737, 1033, 1193
MhlI GDGCHC 3 cut(s) 99, 370, 376
MluCI AATT 7 cut(s) 65, 260, 389, 635, 777, 981, 1007
MlyI GAGTC 5 cut(s) 288, 405, 692, 929, 938
MnlI CCTC 7 cut(s) 26, 29, 71, 211, 490, 694, 1103
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 4 cut(s) 146, 227, 666, 723
MslI CAYNNNNRTG 2 cut(s) 201, 521
Mva1269I GAATGC 1 cut(s) 496
MwoI GCNNNNNNNGC 3 cut(s) 53, 365, 542
NcoI CCATGG 1 cut(s) 88
NdeII GATC 5 cut(s) 255, 404, 546, 615, 831
NlaIII CATG 5 cut(s) 23, 92, 254, 595, 971
NlaIV GGNNCC 2 cut(s) 673, 1174
NmuCI GTSAC 1 cut(s) 712
NspI RCATGY 1 cut(s) 971
PaeR7I CTCGAG 2 cut(s) 276, 1033
PciI ACATGT 1 cut(s) 967
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 120
PfeI GAWTC 5 cut(s) 116, 320, 410, 473, 1038
PkrI GCNGC 3 cut(s) 46, 58, 535
PleI GAGTC 5 cut(s) 287, 404, 692, 928, 938
PpsI GAGTC 5 cut(s) 287, 404, 692, 928, 938
PscI ACATGT 1 cut(s) 967
Psp124BI GAGCTC 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 47
PspN4I GGNNCC 2 cut(s) 673, 1174
PspPI GGNCC 1 cut(s) 595
PspXI VCTCGAGB 1 cut(s) 276
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 2 cut(s) 323, 413
RseI CAYNNNNRTG 2 cut(s) 201, 521
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 4 cut(s) 146, 227, 666, 723
SatI GCNGC 3 cut(s) 45, 57, 534
Sau3AI GATC 5 cut(s) 255, 404, 546, 615, 831
Sau96I GGNCC 1 cut(s) 595
SchI GAGTC 5 cut(s) 288, 405, 692, 929, 938
SduI GDGCHC 3 cut(s) 99, 370, 376
SfaNI GCATC 5 cut(s) 90, 111, 436, 545, 840
SfcI CTRYAG 2 cut(s) 750, 1194
Sfr274I CTCGAG 2 cut(s) 276, 1033
SlaI CTCGAG 2 cut(s) 276, 1033
SmiMI CAYNNNNRTG 2 cut(s) 201, 521
SmlI CTYRAG 2 cut(s) 276, 1033
SmoI CTYRAG 2 cut(s) 276, 1033
SpeI ACTAGT 1 cut(s) 457
Sse9I AATT 7 cut(s) 65, 260, 389, 635, 777, 981, 1007
SsiI CCGC 2 cut(s) 960, 1170
SspMI CTAG 1 cut(s) 458
SstI GAGCTC 1 cut(s) 99
StyI CCWWGG 3 cut(s) 88, 1176, 1200
TaiI ACGT 2 cut(s) 759, 942
TaqI TCGA 6 cut(s) 258, 277, 933, 1011, 1034, 1164
TasI AATT 7 cut(s) 65, 260, 389, 635, 777, 981, 1007
TfiI GAWTC 5 cut(s) 116, 320, 410, 473, 1038
Tru1I TTAA 4 cut(s) 146, 227, 666, 723
Tru9I TTAA 4 cut(s) 146, 227, 666, 723
TscAI CASTG 2 cut(s) 381, 885
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 3 cut(s) 44, 56, 533
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 6 cut(s) 8, 185, 572, 645, 1020, 1203
TspRI CASTG 2 cut(s) 381, 885
XapI RAATTY 5 cut(s) 65, 389, 635, 777, 1007
XceI RCATGY 1 cut(s) 971
XhoI CTCGAG 2 cut(s) 276, 1033
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.