pycom111g03010

TFIIS helical bundle-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0009708
Physical Location & Seq
Reverse (-)
1801385 .. 1804183
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g03010.1

Sequence Viewer

Length: 2721 bp
ATGCTAAAGTCTGAGCTTGGAAAAATAACGGACAAAGGAGGACTACTAGACACACAGGGGGTTGAGAAGTTTGTCCAACTTATGAAAGCTGAAAGTGCTGATAAGAAAATAGATTTGGCTGGCCGAAGAATGCTTGTTGATGTGATAGCAGTTACTGATAGGTTGGATTGCCTTGGGCAATTTGTACAACGCAAGGGTTTGTGTGTGTTGGATGAATGGCTCCAAGAAGTTCACAAGGGAAAACTTGGTGATGGTAGTAGCCCAAAAGAAAGTGATAAATCCGTTGAGGAATTTCTTTTTGCTTTACTTTGTGCACTGGAGAAGGTTCCTGTGAATCTTCATGCGCTGCAGACTTGTAATGTTGGCAAGTCTGTGAATAATTTACGCAGTTACAGAAACTCTGAAATCCAGAAGAAAGCTAGGAGTTTAGTTGATATGTGGAAGAAACGTGTTGAAGCTGAGATGAATTTGAATGAGACAAAGACTGAATCTAGTCGTGGCGGTGTTTCCTGGCCTACAAAGCATGCATCTTCAGAAGTTTCTCATGTAGGGAGCAGAAAGATTGGAAGTTCATCGGAGGTTGGCACAAAAAGTTCTACTATGCAACCTTCTGTGTCTAAAACTCCTCAGATTAAGCTTGGCTGTGGAGAAACAGTTTCTAAATCTTCTGGTTCACCTGGATCAACGAAATTGTTGAGTATATCATCCGGCAATCTCTCAAAGGACCAGAATTTCAGAACATTAGGTGCTGGAACTTCAGATCTTCCATTGACACCAATTAAGGAGGAAAGAAGCAGCAGTTCTAGCCAGTCTCAGAACAACAGCCAGTCAAGTGATCATGCGAGGACTGTGGGTTCTTTATATAGGGAAGATGCAAGGAGCTCGTCTGCTGGTTCAGTTAGTGTGAGCAAAATTTCTGGCAGTGCTTCTCGCCATCGGAAATCAAGCAATGGCCTTAACGGGTCTACTGCCTCAGGAGTTAAGAAGGAATCAGGGCCAGGAAAAGCCTGTACCCTCAATAGAAATTTGACTTTTGAAAAGGCTTCAACAGTTGGAGTATCCTATGAGAAGCTTCCTGAAGTGCCCATGGTTGACCATGGAAATAACCGACTGATTGTCAGATTGCCAAACACTGGTAGGAGTCCTGCTCGTGGTACCTCAGGAGGTTCTTTTGAAGATCTTGTTAACAGAGGGTCTCCTCCTGCTGAAAAGCATGACAACCATGACCAAAAATCTAAGCGCAGAAGCGATGCCTTGCACGTTAATAGCACAGCAGATGTGAACTCAGATGCATTTCACAGCAAAGAGGGATTGTCTGGTTCTGAAGATGGCAATGTGCCAACTTTAGGCAGCGAACAAAATAGGGGTGGTGAAGATGGTGAAAAACAAACAGAAGCATCAAAGGCTACTGGTTCCTCGTCAAAAGTCACTTTAAGGACAGGAAAATCGTATGAAGCATCGTTAAGCTCCATGAATGCGTTAGTTGAAAGCTGTGTGAAGTTTTCTGAAAGTAGTGGCGCTGCTTCACCTGGTGATGATGTTGGGATGAATTTGCTAGCTAGTGTGGCTGCTGGAGAAATGTCCAAATCTGAGAATGTGTCACCATCAGGTTCTCCTGGGAGGAATTCTCCTGTGCCTGAGAGATCATTCTCAGAAGAAGATGCCAAGTTGAAACAACTGGGCAAAGAAATTGCTGACATCCAATGTCAACCTAATGGAGCGGTCAATAGTGGTGCTACTGAGCAGGGCAGTGGATGTGACTCATTGCGAGGTAAGAGTGAAACAAGACACACTCTTACCTACCTGCCAACTAATGGTTTTGCTGATGTTAAAGTTACTTCATCTGGGTGCGGGGATAGAACAGCAGAGTGTAATGCAAATTTGAATTGTTCTTCAAATAAGAAACAAAATACTGATGGTCAATCCTTGAGGGATGATGTGAAGCCTGGTGAGTCATGTGATGCTTCAGCATTAGAGCCACTTTCATGTGCTAGAAAAGAGGGTCATTTGGAGGCTGAAGGATCTAACCAGTCTCATGAGCAAGGAAAATTGGAGGCTTCTAATGATGAAAGAACTGTTGGGAGCAGTACACCAGTTGTGTCTGAAGCAGCCTCTGGGAGTGCAAAAGTTGAACAGGACATTGAAATATCTACTTGCGCATCCTCTCAATTGGCTGGCGAGAACCATGATTTAAAAAAGGATTCGAATAGTGCTATACTGACAGAGCAGAAATCATCTGTTGTTGAAGGAATCCACTCAGAGTCCAAAGAGGGGAAGAGTGAGGATGCTGTTCTATGTTCTGGTTCTGGAAACACTTTACATGTGGAGTTTATGGATGAACAGACAGATGACATTAAGGCTTTCGGTCACACTGCACAGACAGAGAAAGAGACACTTGATAGTTCTGTTCCATTTTTGCAGAACAGCAGAGATTTTGCTCAGGAGACTACAGAAAGAAATGAGGCTTTTGTTCACTGTTCCAATCAACCGGTTTCCAAGGCAGAGTCACTGTCAATATCTGTTATAGAAAATGAGCAGCGTGACAAGTCTAGTAAGTGTAAGTCAGAAGTTATTGAATCAGGGGGGATGGAGGAGCGGCAAGTTTCCTCCGTGAATGCCTCGGCGTCAGGTACTGCTGTAAAGCTGGACTTTGATTTGAACGAGGGCTTTCCTGTTGATGATGGGAGCCAACCAGAGTTTGTTAAAGCAGGAGATCCTTGA

Protein Analysis

907

Amino Acids

96.0

Weight (kDa)

5.59

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med26 PF08711 102 - 151 6.5e-13 TFIIS helical bundle-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2158
Acc36I ACCTGC 1 cut(s) 1812
Acc65I GGTACC 1 cut(s) 1154
AccB1I GGYRCC 1 cut(s) 1154
AccB7I CCANNNNNTGG 2 cut(s) 1133, 1814
AccBSI CCGCTC 2 cut(s) 1721, 2596
AccI GTMKAC 1 cut(s) 965
AciI CCGC 4 cut(s) 501, 1721, 1851, 2596
AclWI GGATC 3 cut(s) 688, 2029, 2708
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 8 cut(s) 290, 466, 730, 912, 1024, 1549, 1624, 1879
AcuI CTGAAG 7 cut(s) 516, 741, 1098, 1344, 1950, 2037, 2124
AcyI GRCGYC 1 cut(s) 2624
AdeI CACNNNGTG 1 cut(s) 1532
AfaI GTAC 5 cut(s) 186, 1012, 1156, 2089, 2632
AfiI CCNNNNNNNGG 5 cut(s) 1133, 1151, 1346, 1814, 2271
AflIII ACRYGT 2 cut(s) 448, 2320
AgeI ACCGGT 1 cut(s) 2488
AhdI GACNNNNNGTC 1 cut(s) 1115
AjnI CCWGG 6 cut(s) 509, 676, 997, 1528, 1615, 1945
AjuI GAANNNNNNNTTGG 4 cut(s) 98, 130, 2061, 2093
AloI GAACNNNNNNTCC 2 cut(s) 838, 870
Alw21I GWGCWC 2 cut(s) 316, 884
Alw26I GTCTC 6 cut(s) 470, 816, 1200, 2037, 2384, 2438
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 3 cut(s) 688, 2029, 2708
AlwNI CAGNNNCTG 3 cut(s) 155, 2114, 2633
AoxI GGCC 4 cut(s) 121, 512, 952, 995
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 7 cut(s) 346, 795, 1350, 1520, 1568, 2108, 2536
ApoI RAATTY 8 cut(s) 290, 466, 730, 912, 1024, 1549, 1624, 1879
AsiGI ACCGGT 1 cut(s) 2488
Asp718I GGTACC 1 cut(s) 1154
AspLEI GCGC 4 cut(s) 346, 1242, 1520, 2159
AspS9I GGNCC 2 cut(s) 724, 995
AsuHPI GGTGA 8 cut(s) 260, 666, 1382, 1391, 1518, 1544, 1593, 1961
AsuII TTCGAA 1 cut(s) 2204
AsuNHI GCTAGC 1 cut(s) 1555
AvaII GGWCC 1 cut(s) 724
AxyI CCTNAGG 2 cut(s) 973, 1159
BaeGI GKGCMC 2 cut(s) 316, 1086
BaeI ACNNNNGTAYC 2 cut(s) 2622, 2655
BanI GGYRCC 1 cut(s) 1154
BanII GRGCYC 1 cut(s) 884
BauI CACGAG 1 cut(s) 1149
Bbv12I GWGCWC 2 cut(s) 316, 884
BbvI GCAGC 7 cut(s) 333, 807, 1362, 1507, 1555, 2120, 2548
BccI CCATC 8 cut(s) 245, 942, 1322, 1370, 1612, 1910, 2581, 2675
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciT130I CCWGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
BciVI GTATCC 1 cut(s) 1069
BclI TGATCA 1 cut(s) 835
BcoDI GTCTC 6 cut(s) 470, 816, 1200, 2037, 2384, 2438
BfaI CTAG 8 cut(s) 47, 420, 492, 804, 1556, 1560, 1992, 2550
BfmI CTRYAG 2 cut(s) 347, 2448
BfoI RGCGCY 1 cut(s) 1521
BfuAI ACCTGC 1 cut(s) 1812
BfuI GTATCC 1 cut(s) 1069
BglII AGATCT 2 cut(s) 760, 1177
BisI GCNGC 8 cut(s) 347, 796, 1351, 1521, 1569, 2109, 2537, 2597
BlsI GCNGC 8 cut(s) 348, 797, 1352, 1522, 1570, 2110, 2538, 2598
Bme1390I CCNGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
Bme18I GGWCC 1 cut(s) 724
BmeRI GACNNNNNGTC 1 cut(s) 1115
BmgT120I GGNCC 2 cut(s) 724, 995
BmiI GGNNCC 5 cut(s) 221, 327, 1156, 1414, 2687
BmrFI CCNGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
BmrI ACTGGG 1 cut(s) 1688
BmtI GCTAGC 1 cut(s) 1559
BmuI ACTGGG 1 cut(s) 1688
BplI GAGNNNNNCTC 4 cut(s) 1132, 1164, 1612, 1644
BpmI CTGGAG 2 cut(s) 338, 1593
Bpu10I CCTNAGC 1 cut(s) 2439
Bpu14I TTCGAA 1 cut(s) 2204
BpuEI CTTGAG 1 cut(s) 1948
BsaHI GRCGYC 1 cut(s) 2624
BsaI GGTCTC 1 cut(s) 1200
BsaJI CCNNGG 6 cut(s) 172, 1086, 1096, 1616, 2496, 2619
BsaWI WCCGGW 1 cut(s) 2488
BsaXI ACNNNNNCTCC 2 cut(s) 639, 669
Bsc4I CCNNNNNNNGG 5 cut(s) 1133, 1151, 1346, 1814, 2271
Bse118I RCCGGY 1 cut(s) 2488
Bse1I ACTGG 8 cut(s) 321, 808, 826, 1138, 1414, 1683, 2029, 2093
Bse21I CCTNAGG 2 cut(s) 973, 1159
Bse3DI GCAATG 3 cut(s) 955, 1339, 1763
BseBI CCWGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
BseDI CCNNGG 6 cut(s) 172, 1086, 1096, 1616, 2496, 2619
BseLI CCNNNNNNNGG 5 cut(s) 1133, 1151, 1346, 1814, 2271
BseMI GCAATG 3 cut(s) 955, 1339, 1763
BseNI ACTGG 8 cut(s) 321, 808, 826, 1138, 1414, 1683, 2029, 2093
BseRI GAGGAG 3 cut(s) 615, 1188, 2606
BseSI GKGCMC 2 cut(s) 316, 1086
BseXI GCAGC 7 cut(s) 333, 807, 1362, 1507, 1555, 2120, 2548
BsgI GTGCAG 1 cut(s) 2358
BshFI GGCC 4 cut(s) 123, 514, 954, 997
BshNI GGYRCC 1 cut(s) 1154
BshTI ACCGGT 1 cut(s) 2488
BsiHKAI GWGCWC 2 cut(s) 316, 884
BsiSI CCGG 2 cut(s) 708, 2489
BslI CCNNNNNNNGG 5 cut(s) 1133, 1151, 1346, 1814, 2271
BsmAI GTCTC 6 cut(s) 470, 816, 1200, 2037, 2384, 2438
BsmI GAATGC 3 cut(s) 135, 1480, 2620
BsnI GGCC 4 cut(s) 123, 514, 954, 997
Bso31I GGTCTC 1 cut(s) 1200
Bsp119I TTCGAA 1 cut(s) 2204
Bsp1286I GDGCHC 3 cut(s) 316, 884, 1086
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
Bsp19I CCATGG 2 cut(s) 1086, 1096
BspACI CCGC 4 cut(s) 501, 1721, 1851, 2596
BspANI GGCC 4 cut(s) 123, 514, 954, 997
BspHI TCATGA 1 cut(s) 2035
BspLI GGNNCC 5 cut(s) 221, 327, 1156, 1414, 2687
BspMAI CTGCAG 1 cut(s) 351
BspMI ACCTGC 1 cut(s) 1812
BspOI GCTAGC 1 cut(s) 1559
BspPI GGATC 3 cut(s) 688, 2029, 2708
BspT104I TTCGAA 1 cut(s) 2204
BspT107I GGYRCC 1 cut(s) 1154
BspTNI GGTCTC 1 cut(s) 1200
BsrBI CCGCTC 2 cut(s) 1721, 2596
BsrDI GCAATG 3 cut(s) 955, 1339, 1763
BsrFI RCCGGY 1 cut(s) 2488
BsrGI TGTACA 1 cut(s) 184
BsrI ACTGG 8 cut(s) 321, 808, 826, 1138, 1414, 1683, 2029, 2093
BssAI RCCGGY 1 cut(s) 2488
BssECI CCNNGG 6 cut(s) 172, 1086, 1096, 1616, 2496, 2619
BssMI GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
BssNI GRCGYC 1 cut(s) 2624
BssSI CACGAG 1 cut(s) 1149
BssT1I CCWWGG 4 cut(s) 172, 1086, 1096, 2496
Bst2BI CACGAG 1 cut(s) 1149
Bst2UI CCWGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
Bst4CI ACNGT 6 cut(s) 655, 850, 1051, 2077, 2477, 2511
Bst6I CTCTTC 1 cut(s) 2270
BstACI GRCGYC 1 cut(s) 2624
BstAUI TGTACA 1 cut(s) 184
BstBI TTCGAA 1 cut(s) 2204
BstC8I GCNNGC 4 cut(s) 121, 525, 1557, 2176
BstDSI CCRYGG 2 cut(s) 1086, 1096
BstH2I RGCGCY 1 cut(s) 1521
BstHHI GCGC 4 cut(s) 346, 1242, 1520, 2159
BstKTI GATC 7 cut(s) 683, 763, 838, 1180, 1646, 2024, 2716
BstMAI GTCTC 6 cut(s) 470, 816, 1200, 2037, 2384, 2438
BstMBI GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
BstMWI GCNNNNNNNGC 5 cut(s) 95, 520, 804, 1403, 1565
BstNI CCWGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
BstNSI RCATGY 2 cut(s) 527, 2324
BstSCI CCNGG 6 cut(s) 509, 676, 997, 1528, 1615, 1945
BstSFI CTRYAG 2 cut(s) 347, 2448
BstSLI GKGCMC 2 cut(s) 316, 1086
BstV1I GCAGC 7 cut(s) 333, 807, 1362, 1507, 1555, 2120, 2548
BstX2I RGATCY 4 cut(s) 760, 1177, 2021, 2713
BstYI RGATCY 4 cut(s) 760, 1177, 2021, 2713
Bsu36I CCTNAGG 2 cut(s) 973, 1159
BsuI GTATCC 1 cut(s) 1069
BsuRI GGCC 4 cut(s) 123, 514, 954, 997
BtgI CCRYGG 2 cut(s) 1086, 1096
BtgZI GCGATG 1 cut(s) 1263
BtsI GCAGTG 3 cut(s) 928, 1756, 2370
BtsIMutI CAGTG 7 cut(s) 314, 928, 1131, 1756, 2370, 2473, 2507
BveI ACCTGC 1 cut(s) 1812
Cac8I GCNNGC 4 cut(s) 121, 525, 1557, 2176
CaiI CAGNNNCTG 3 cut(s) 155, 2114, 2633
CciI TCATGA 1 cut(s) 2035
CfoI GCGC 4 cut(s) 346, 1242, 1520, 2159
Cfr10I RCCGGY 1 cut(s) 2488
Cfr13I GGNCC 2 cut(s) 724, 995
CseI GACGC 1 cut(s) 2613
CsiI ACCWGGT 1 cut(s) 1528
Csp6I GTAC 5 cut(s) 185, 1011, 1155, 2088, 2631
CspAI ACCGGT 1 cut(s) 2488
CviQI GTAC 5 cut(s) 185, 1011, 1155, 2088, 2631
DpnI GATC 7 cut(s) 682, 762, 837, 1179, 1645, 2023, 2715
DpnII GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
DraI TTTAAA 1 cut(s) 2193
DraIII CACNNNGTG 1 cut(s) 1532
DriI GACNNNNNGTC 1 cut(s) 1115
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 2270
Eam1105I GACNNNNNGTC 1 cut(s) 1115
EarI CTCTTC 1 cut(s) 2270
Ecl136II GAGCTC 1 cut(s) 882
Eco130I CCWWGG 4 cut(s) 172, 1086, 1096, 2496
Eco24I GRGCYC 1 cut(s) 884
Eco31I GGTCTC 1 cut(s) 1200
Eco47I GGWCC 1 cut(s) 724
Eco53kI GAGCTC 1 cut(s) 882
Eco57I CTGAAG 7 cut(s) 516, 741, 1098, 1344, 1950, 2037, 2124
Eco81I CCTNAGG 2 cut(s) 973, 1159
EcoICRI GAGCTC 1 cut(s) 882
EcoRI GAATTC 1 cut(s) 1624
EcoRII CCWGG 6 cut(s) 509, 676, 997, 1528, 1615, 1945
EcoT14I CCWWGG 4 cut(s) 172, 1086, 1096, 2496
EcoT22I ATGCAT 2 cut(s) 529, 1294
EcoT38I GRGCYC 1 cut(s) 884
ErhI CCWWGG 4 cut(s) 172, 1086, 1096, 2496
FalI AAGNNNNNCTT 4 cut(s) 2379, 2411, 2633, 2665
FauI CCCGC 1 cut(s) 1844
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 1 cut(s) 965
Fnu4HI GCNGC 8 cut(s) 347, 796, 1351, 1521, 1569, 2109, 2537, 2597
FriOI GRGCYC 1 cut(s) 884
Fsp4HI GCNGC 8 cut(s) 347, 796, 1351, 1521, 1569, 2109, 2537, 2597
FspBI CTAG 8 cut(s) 47, 420, 492, 804, 1556, 1560, 1992, 2550
FspI TGCGCA 1 cut(s) 2158
GlaI GCGC 4 cut(s) 345, 1241, 1519, 2158
GluI GCNGC 8 cut(s) 347, 796, 1351, 1521, 1569, 2109, 2537, 2597
GsuI CTGGAG 2 cut(s) 338, 1593
HaeII RGCGCY 1 cut(s) 1521
HaeIII GGCC 4 cut(s) 123, 514, 954, 997
HapII CCGG 2 cut(s) 708, 2489
HgaI GACGC 1 cut(s) 2613
HhaI GCGC 4 cut(s) 346, 1242, 1520, 2159
Hin1I GRCGYC 1 cut(s) 2624
Hin6I GCGC 4 cut(s) 344, 1240, 1518, 2157
HinP1I GCGC 4 cut(s) 344, 1240, 1518, 2157
HincII GTYRAC 3 cut(s) 1093, 1186, 1709
HindII GTYRAC 3 cut(s) 1093, 1186, 1709
HindIII AAGCTT 2 cut(s) 635, 1070
HpaI GTTAAC 1 cut(s) 1186
HpaII CCGG 2 cut(s) 708, 2489
HphI GGTGA 8 cut(s) 260, 666, 1382, 1391, 1518, 1544, 1593, 1961
Hpy188III TCNNGA 7 cut(s) 409, 975, 1076, 1161, 2036, 2307, 2441
HpyAV CCTTC 5 cut(s) 316, 618, 979, 2012, 2240
HpyCH4III ACNGT 6 cut(s) 655, 850, 1051, 2077, 2477, 2511
HpyCH4IV ACGT 2 cut(s) 448, 1260
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 520, 804, 1403, 1565
HpySE526I ACGT 2 cut(s) 448, 1260
Hsp92I GRCGYC 1 cut(s) 2624
HspAI GCGC 4 cut(s) 344, 1240, 1518, 2157
KpnI GGTACC 1 cut(s) 1158
Ksp22I TGATCA 1 cut(s) 835
KspAI GTTAAC 1 cut(s) 1186
Kzo9I GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
LmnI GCTCC 8 cut(s) 225, 552, 879, 1472, 1718, 2082, 2593, 2685
Lsp1109I GCAGC 7 cut(s) 333, 807, 1362, 1507, 1555, 2120, 2548
MabI ACCWGGT 1 cut(s) 1528
MaeI CTAG 8 cut(s) 47, 420, 492, 804, 1556, 1560, 1992, 2550
MaeII ACGT 2 cut(s) 448, 1260
MaeIII GTNAC 9 cut(s) 151, 389, 1426, 1599, 1757, 1834, 2366, 2505, 2540
MalI GATC 7 cut(s) 682, 762, 837, 1179, 1645, 2023, 2715
MbiI CCGCTC 2 cut(s) 1721, 2596
MboI GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
MfeI CAATTG 1 cut(s) 2168
MflI RGATCY 4 cut(s) 760, 1177, 2021, 2713
MhlI GDGCHC 3 cut(s) 316, 884, 1086
MlyI GAGTC 5 cut(s) 1150, 1754, 1961, 2270, 2513
MmeI TCCRAC 4 cut(s) 100, 144, 189, 1033
Mph1103I ATGCAT 2 cut(s) 529, 1294
MslI CAYNNNNRTG 2 cut(s) 1846, 1984
MspI CCGG 2 cut(s) 708, 2489
MspR9I CCNGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
MunI CAATTG 1 cut(s) 2168
Mva1269I GAATGC 3 cut(s) 135, 1480, 2620
MvaI CCWGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
MwoI GCNNNNNNNGC 5 cut(s) 95, 520, 804, 1403, 1565
NcoI CCATGG 2 cut(s) 1086, 1096
NdeII GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
NheI GCTAGC 1 cut(s) 1555
NlaIV GGNNCC 5 cut(s) 221, 327, 1156, 1414, 2687
NmeAIII GCCGAG 1 cut(s) 2600
NmuCI GTSAC 6 cut(s) 1426, 1599, 1757, 2366, 2505, 2540
NsbI TGCGCA 1 cut(s) 2158
NsiI ATGCAT 2 cut(s) 529, 1294
NspI RCATGY 2 cut(s) 527, 2324
NspV TTCGAA 1 cut(s) 2204
PaeI GCATGC 1 cut(s) 527
PagI TCATGA 1 cut(s) 2035
PciI ACATGT 1 cut(s) 2320
PctI GAATGC 3 cut(s) 135, 1480, 2620
PfeI GAWTC 6 cut(s) 334, 488, 989, 2201, 2250, 2576
PflMI CCANNNNNTGG 2 cut(s) 1133, 1814
PinAI ACCGGT 1 cut(s) 2488
PkrI GCNGC 8 cut(s) 348, 797, 1352, 1522, 1570, 2110, 2538, 2598
PleI GAGTC 5 cut(s) 1149, 1754, 1960, 2269, 2512
PpsI GAGTC 5 cut(s) 1149, 1754, 1960, 2269, 2512
PscI ACATGT 1 cut(s) 2320
Psp124BI GAGCTC 1 cut(s) 884
Psp6I CCWGG 6 cut(s) 509, 676, 997, 1528, 1615, 1945
PspGI CCWGG 6 cut(s) 509, 676, 997, 1528, 1615, 1945
PspN4I GGNNCC 5 cut(s) 221, 327, 1156, 1414, 2687
PspPI GGNCC 2 cut(s) 724, 995
PstI CTGCAG 1 cut(s) 351
PstNI CAGNNNCTG 3 cut(s) 155, 2114, 2633
PsuI RGATCY 4 cut(s) 760, 1177, 2021, 2713
RsaI GTAC 5 cut(s) 186, 1012, 1156, 2089, 2632
RsaNI GTAC 5 cut(s) 185, 1011, 1155, 2088, 2631
RseI CAYNNNNRTG 2 cut(s) 1846, 1984
SacI GAGCTC 1 cut(s) 884
SatI GCNGC 8 cut(s) 347, 796, 1351, 1521, 1569, 2109, 2537, 2597
Sau3AI GATC 7 cut(s) 680, 760, 835, 1177, 1643, 2021, 2713
Sau96I GGNCC 2 cut(s) 724, 995
SchI GAGTC 5 cut(s) 1150, 1754, 1961, 2270, 2513
ScrFI CCNGG 6 cut(s) 511, 678, 999, 1530, 1617, 1947
SduI GDGCHC 3 cut(s) 316, 884, 1086
SexAI ACCWGGT 1 cut(s) 1528
SfcI CTRYAG 2 cut(s) 347, 2448
SfuI TTCGAA 1 cut(s) 2204
SinI GGWCC 1 cut(s) 724
SmiMI CAYNNNNRTG 2 cut(s) 1846, 1984
SmlI CTYRAG 1 cut(s) 1927
SmoI CTYRAG 1 cut(s) 1927
SphI GCATGC 1 cut(s) 527
SsiI CCGC 4 cut(s) 501, 1721, 1851, 2596
SspMI CTAG 8 cut(s) 47, 420, 492, 804, 1556, 1560, 1992, 2550
SstI GAGCTC 1 cut(s) 884
StyD4I CCNGG 6 cut(s) 509, 676, 997, 1528, 1615, 1945
StyI CCWWGG 4 cut(s) 172, 1086, 1096, 2496
TaaI ACNGT 6 cut(s) 655, 850, 1051, 2077, 2477, 2511
TaiI ACGT 2 cut(s) 451, 1263
TaqI TCGA 1 cut(s) 2204
TaqII GACCGA 1 cut(s) 2354
TatI WGTACW 2 cut(s) 184, 2087
TauI GCSGC 1 cut(s) 2599
TfiI GAWTC 6 cut(s) 334, 488, 989, 2201, 2250, 2576
TscAI CASTG 7 cut(s) 321, 928, 1138, 1756, 2377, 2480, 2514
TseFI GTSAC 6 cut(s) 1426, 1599, 1757, 2366, 2505, 2540
TseI GCWGC 7 cut(s) 346, 795, 1350, 1520, 1568, 2108, 2536
Tsp45I GTSAC 6 cut(s) 1426, 1599, 1757, 2366, 2505, 2540
TspGWI ACGGA 3 cut(s) 44, 271, 2599
TspRI CASTG 7 cut(s) 321, 928, 1138, 1756, 2377, 2480, 2514
Van91I CCANNNNNTGG 2 cut(s) 1133, 1814
VneI GTGCAC 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 724
XapI RAATTY 8 cut(s) 290, 466, 730, 912, 1024, 1549, 1624, 1879
XceI RCATGY 2 cut(s) 527, 2324
XmiI GTMKAC 1 cut(s) 965
XspI CTAG 8 cut(s) 47, 420, 492, 804, 1556, 1560, 1992, 2550
Zsp2I ATGCAT 2 cut(s) 529, 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.