Rroxscaffold_2G00081180

TFIIS helical bundle-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009708
Physical Location & Seq
Reverse (-)
4154731 .. 4166462
11732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081180.1

Sequence Viewer

Length: 6066 bp
ATGCATGGGTCGCCAGCTGAGGAGTCGATACGGCGTCGTCGGCACATGTGGCCTGTCCCTCATTCCAACGCCACGACTGTAGCTTCCAACCCCTCTTCACCACCACCGCCACCTGATTTCTTCAACAAAGATGGACGCAAAATTCGTGTTGGAGATTGTGCACTTTTCAAGCCTCCTCAGGATTCCCCACCTTTTATAGGAATAATTCGCTGGCTGAAACTAGACAAGGGAGATAGTTTGAGTTTAGGGGTTAATTGGCTCTATCGACCTGCTGATGTGAAGCTTCTTAAAGGGGTCTCGCCAGAAGCTGCCCCAAACGAGGTCTTCTACTCCTTTCACAAGGATGAGATACCTGCTGCATCACTACTGCATCCGTGTAAAGTCGCATTCCTTCGTAAAGGTGTTGAACTTCCTTCAGGGATATCCTCATTTGTGTGCAGAAGAGTGTATGATACTGAGAACAAGTGTTTATGGTGGTTAACTGACAAAGATTATATTAATGAACGACAGGAAGAAGTAGATCATCTTTTAGACAAGACAAGACTAGAAATGCATGGGGCAGTGCAGTCGGGAGGTCGTTCTCCGAAGCCCTTGAATGGTCCGTCATCAACACCACAGTTCAAATCTGGTTCAGATAGTTTACAGAATAGTGCATCCCCCTTTTCTTCGCAGGGTAAGGGAAAAAAGAGGGAACGAGGTGATCAGAGCTCCGATCCGGTGAAACGTGAGCGCTTAATTAAGACAGAGGATGGAGAGGCTGGCCACGCCAGACCGGAAAGTGTATTGAAGTCTGAGCTTTCCAAAATAACAGACAAAGGAGGACTTGTAGACTTAGAGGCGGTTGAGAAGTTAGTCCAACTTATGCAACCTGACAGTGCTGAAAAGAAAATAGATATGGCTGGCCGAATATTGCTTGTTGATGTCATAGCAGTTACTGATAGGTTTGATTGCCTTGGGCGATTTGTACAGCTAAGGGGTTTGGCTGTATTGGATGAATGGCTTCAAGAGGTTCACAAGGGAAAAATTGGTGATGGTAGTAGCCCTAAAGAAAGTGATAAATCTGTTGAGGAGTTTCTCTTTGCATTACTTCGTGCACTGGATAAGCTGCCTGTGAATCTTCATGCTCTACAAACTTGTAATGTTGGCAAGTCTGTGAATAATTTACGTACACACAAGAACTCTGAAATTCAGAAGAAAGCTAGGAGTTTGGTTGACACATGGAAGAAGCGTGTAGAAGCTGAGATGAAATTAAATGAATCAAAGTCTGGATCAAGCCGTGGTGTCTCCTGGCCTTCAAAGGCTGCACCTTCAGAAGTTTCTCAGGTTGGCAGCAGAAAAACTGGAAGTTCATCTGAGGTTGGCTCAAAAAGCTCCACTGTTCAAAATTCCGTGTCCAAAGCTCCACAAGTGAAAGTTGGCTCTTCTGAAATGGTTTCAAAGTCTTCTGCTTCTCCTGGGTCAACAAAACTGCTGTCTGTATCCTCTGGTAATATCTCTAAGGATCAAAATTTCAGAATGCTAGTTGGTGCTGGAAACTCCGATCTTCCATTGACACCAATTAAGGAGGAACGGAGCAGCAGTTCTAGTCAGTCTCAGAACAACAGCCAATCAAGTGATCATGCAAAGACTGTGGGTTCCTTATATAAAGAAGATGCAAGAAGCTCAAGTGCTGGTTCAGTAAGTGCAAATAAACTTTCTAGCGGCTCTTCTCGACATCGGAAATCAAGCAATGGTCTTCATGGGTCTGCTGGCTCAGGGGTGCATAAGGAAGCAGGACCAGGAAAAGTTGGTACCCCAAGTAGAAATTTGACTTCTGAGAAGGCATCAACCGCCGGAGCATCCTATGAGAAAGGTCATGAAGCTCCAATGGTTGACCAGGCAAGTAGCAGACTAATTGTGAGGCTGCCGAACACTGGCCGCAGTCCAGCCCGGGGTGCTTCAGGTGGTTCTTTTGAAGATCCTGTTACATCTGGCAGAGCTTCTCCTTCTGCAGAAAAGCATGGCAACCAGGAGAAAAAAGCCAAGGGTAGAAGTGATGCTTTGCTGGGAAATAGTACTTCCGATATGAACTCAGATGTATGTCACAGCAAAGATGGATTGTCTGGTTCTGAAGAAAATAATGTGCCACCTTTCTCCAGTGAACAAAATAGGGCTGGTGAAGATGCTGAAAAACCAATGGAGGCATCAAAGGTTGCTGGTCCTGGATCAAAAGTTATATCAAGGATGGGAAAATCATATGAAGCATCCTTAAGCTCCATGAATGCTTTAATTGAAAGTTGTGTCAAGTTTTCTGAAGGTAGTGACACTGCTTCACCTGGAGATGATGTTGGGATGAATTTACTAGCTAGTGTGGCGGCTGGAGAGCTGTCCAAATCTGAAAATGTCTCACCATCATGTTCTCCCCAGAGGAATTCTCCTGTGCCTGATGGTTCATTTTCAGATAAAGATGCCAAGTTGAAACAACTGGGTGAAGTATCTGAAACCCAGTGTCAACCTATCGGCAGGGCTAATAGTGGTTCTACTGCAGAGCTGGGAAACGCTGGTGACTCATTGCGGGGTAAGAGTGAACCAAGACATTCCGTACCCCATCTGCCAGCTAATGTTTCTGGAGGAGACACCAAATGCAGTTCAGCTGGCTCTGGGAATCAAATAGTCGAGTGTAATGCAAATTTGAATTGTTCTTCCAATATGCAACACAATGTAGATAGACCATCCTTGGCGGTTGATGGGAAGCCTGTTGAGCCATATGATGCTTCTGGATCAGAGCTACCTTCATGCGCTACAAAAGAGGGTCTTGTGGAGGCTGAAGGGTGTAATCAGTCTCATGAGCAAGGAAAATTAGGGGCGCAGAATGCGAAAGGATGCAGTATTTCTGAGTCTAAATTAAAAGTGCCGCCTGCCTTGCATGATGAGGATAAAATTCTACATAAAGGAGATGAAAGAACTGTGGGAAGCAGTAAACCTATTGTGTCTGAAGCAACCTCTGGGAGTGAAAAAGTTGAAAAGGACAATGAAACATCAACCTGCTCGTCCTCTGAAATGGCTGATGACAACCCAGATACCGTTAAGGATTCAAATATTGCCCTATTGGCAGAGCAGAAACCATCTGGTGCAACAGGAATTCAATCAGAGTCGAAGGAGGGGAAGAGTGAGGATGCTGTTCCTCGTTCTGGTTCTGGAAATACTTTACAGTTGCAGTTGAAGGGCGAAAACACAGATGAAGATAAGGCCATGGGTCACTCTGAGCAGACTGTGAAAGACGAAAGGGATAAGAGTATGGAAAGAAAAGACGCTTTGGAACACAGCAGCGAATTCTCTCAGGAGATTAAGGAAAGAAAGGAGACTTCTGGTCAGTGTTCTGGGATACCAATTCCTCGTGTTCAGTCACCTTCAGTACCCGTGCAAGAAAATCACAAGCCTGGGTGCAAGTTGGAAGCTATTGAATCAGGGGAAAAAGAGGAGCGGCAAATTTCAGGTGTGAATGCCTCGGGGTCAGATACTGCTGTAAAGCTGGACTTTGATTTGAACGAAGGTTTCCCTGTTGATGATAGTATTCAACAAGAGTTTGTTAAAGCAGGAGATCCTGGAGCTTCATCTTCTGTTCATGTTCCTTGCCCTTTACCCTTCCAAATGCCTTCCATGTCGGGGAGTTTCCCTGCATCAGTTACTGTGGTTGCACCTGCCAAAGGTTCATTTGTCCCTCCCGAAAACCCAATGCGGACACCACTCAGCACAAGCGATCCACCTGTTGTTGATACTGCTTCTAGCAAACAAGGCCGCCCACCATTGGACTTTGATCTCAATGTGCCTGACCAGAGAGTTTATGAGGATGTTGTTTCCCAAAACCCTGCACATGTCATGGATCACAAGTCTGGGTCTCATGATCGCGGTGCTGGAGGGCTTGATCTCGATTTGAATAGAGTTGATGAGAGTCCTGATATCGTTCCGCTTCCTGTTATCAACAGTTGTAGGTTAGAGATTCCTCCTTTGCCAAGTAGGTCATCATTATCTGGTGGACTATCTAATGGTGGAATTAATGACTCCAGAGATTTTGATTTGAACAATGGCCCCGGCCTTGATGAAGTGGGGACTGAAGCTGCACCATTTACCCAGCATATAAAAAGCAGTGTGCCTCTGCGAACACCTGTTTCTGGGTTAAGAATGAATAGCCCTGACTTTGGGAATTTTTCAGCATGGTTCGCTCCTGGAAACTCATATCCTGCCATCACAGTCCCATCCATTTTTCCAGGCAGAGGTGAGCAGAGTTATGGTACTGCTGCTGGGTCACAGAGAGTCTTGTGTCCTCCCACTGGTAACCCCTCATTTGGTCCTGAAATCTACAGGGGCCTGTACTGTCGTTGTTCAACTGCATATGTGGATTCATCATCTGGTGGGGCACTCTGCTTTCCCACCATGCCTTCACAGCTCATGGGACCAGGTGGTGTAGTCTCATCACCTTACCCACGACCCTATATGATGAACCTTGCTGGCAGCAGCAGTAATGTTGGCCTTGATGGTAGGAAATGGGGTAGTCAGGGCCTTGACCTCAATTCCGGGCCTGGAAGTACAGAAGCAGAACGGAGAGATGAGAGGTTGCCATCAGGGTTGAGGCAGCTTTCTGTTCCCAGTTCACAAGCCCTTGTAGAGGAACAGTTGAAGATGTTTCAGGGTCTTGCATTGAAACGTACAGCAGATGTGGAGCTCAAGAGGCAAATGCTAAAGGGCAGGAACATTTTGTTCATTGTGCGGCAGTCCAACTTTGGAAAACCCTGGTGCCCTGGAGGCGATTTATTTAAAGGATCTATTCTGATGGCCTCTGAAGACGAGGTTAGTATCAAAGTTCTGGTTAGATTCATGTACCTGTACTGCAAAACCACTTATTCTCCCGTAGCCTCCATTTTCAGCTTCTTCCTTGCTTATTCCCCAAGCTCTTTTCCGATCACATCCAAACAAGCCCTTACTTCTGTTCAGAACCAGCCAGATGATGATAATCCTAGCCATGCAGTACCAGTCAAGATGATCCCACCTCCAAAAAAGGGAGAGGAGTGTGACTTGTTTAAGGGTCAGTGGGTTCCGGATTTGAGAGGGCCATTGTATTCGAATTCGAGCTGTACGAGTATTCCGGAATCGAAGAATTGTTTCAAGAATGGGAGGATGGATAGGGATTTTCTGAATTGGAAATGGAAGCCGGAGAAATGTGAGCTGCCGGAGTTTGATCCCAAGGCGTTCTTGCAAATTGTTCGAGGGAAGAAGATGGCATTTGTTGGTGACTCTGTTGCTCGCAATCACGTTGAGTCTCTTCTATGCCTCTTGTCACAGGAGGAACTTCCCAAAGACATCTACAAGGATTCTGAAGACCGTTTCAGGACATGGTACTTTCCTCAACATGACTTCACCCTAATGAAATTGTGGTCCAAATATCTTATAGCCGCAGACGAGAGGATGGTTAATGGCACTGGTTCAGGCGTTTTTGATCTGCACATGGACAGAATCGATGATGAATGGGCAAAGCACCTTCCGGGTTTAGACTACGCCATCATCTCGGACGGGCATTGGTTTTTTCGGGTATTATACCTATACAAAGGCAAAAAACTCATAGGATGTGTCTATTGCAACCAACCCAATATTACGGGCTTGAACGCTAGCAATGCAGTTCGCATGGCGTTTCGATCTGCATTTAAATACATTAGCAATTGCAAGAATTGCAAGGGCGGGCTTCTGACCTTGTTGAGGACATTTGCACCGGCACATTTTGAGAACGGGGCTTGGAACACTGGAGGGTACTGCAATAGGACGAGTCCCTTACGTGAGGCACAAGTTGATTTTACGAAGTTTGATTGGGAAATGAGGAAGATTCAGATTGAGGAGATTGAAAGAGCAAGAAAAGAAGGGAAGAAGATTAAAGTTTTGGATATAACTAGGGCAATGTTGATGAGACCAGACGGGCACCCTGGACTTCACTGGGGAAATCAATGGATGAAGGGTTATAGTGACTGTGTTCATTGGTGCATGCCAGGCCCGGTTGATTACTGGAACAATTTTTTGATGGCACTTCTCAGAAATTAA

Protein Analysis

2021

Amino Acids

218.77

Weight (kDa)

6.77

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAH PF01426 47 - 155 2e-13 BAH domain
Med26 PF08711 362 - 411 2.8e-14 TFIIS helical bundle-like domain
PMR5N PF14416 1681 - 1734 7.4e-20 PMR5 N terminal Domain
PC-Esterase PF13839 1735 - 2017 6.6e-91 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3688
Acc36I ACCTGC 4 cut(s) 277, 361, 3032, 3688
Acc65I GGTACC 1 cut(s) 1790
AccB1I GGYRCC 3 cut(s) 1790, 4773, 5946
AccBSI CCGCTC 1 cut(s) 3463
AccI GTMKAC 1 cut(s) 828
AccII CGCG 1 cut(s) 3888
AccIII TCCGGA 2 cut(s) 5074, 5122
AcoI YGGCCR 3 cut(s) 760, 901, 1915
AcyI GRCGYC 1 cut(s) 34
AfeI AGCGCT 1 cut(s) 731
AflII CTTAAG 1 cut(s) 2248
AflIII ACRYGT 2 cut(s) 45, 3853
AhdI GACNNNNNGTC 1 cut(s) 3348
AloI GAACNNNNNNTCC 2 cut(s) 1564, 1596
Alw21I GWGCWC 4 cut(s) 163, 710, 1096, 4704
Alw44I GTGCAC 2 cut(s) 159, 1092
AlwNI CAGNNNCTG 4 cut(s) 308, 935, 3500, 3668
Ama87I CYCGRG 2 cut(s) 1929, 3487
Aor13HI TCCGGA 2 cut(s) 5074, 5122
Aor51HI AGCGCT 1 cut(s) 731
ApaLI GTGCAC 2 cut(s) 159, 1092
ArsI GACNNNNNNTTYG 4 cut(s) 126, 158, 1457, 1489
AseI ATTAAT 2 cut(s) 498, 4035
Asp700I GAANNNNTTC 2 cut(s) 999, 5138
Asp718I GGTACC 1 cut(s) 1790
AspLEI GCGC 3 cut(s) 732, 2780, 2848
AsuC2I CCSGG 6 cut(s) 1930, 1931, 4071, 4555, 5520, 6020
AsuII TTCGAA 1 cut(s) 5099
AsuNHI GCTAGC 1 cut(s) 5642
AvaI CYCGRG 2 cut(s) 1929, 3487
AvaII GGWCC 6 cut(s) 599, 1775, 2198, 4328, 4433, 5412
AxyI CCTNAGG 1 cut(s) 177
BaeGI GKGCMC 5 cut(s) 163, 1096, 4399, 4778, 5949
BaeI ACNNNNGTAYC 2 cut(s) 3489, 3522
BalI TGGCCA 1 cut(s) 762
BanI GGYRCC 3 cut(s) 1790, 4773, 5946
BanII GRGCYC 2 cut(s) 710, 4704
BauI CACGAG 1 cut(s) 3375
BbsI GAAGAC 5 cut(s) 316, 1434, 1727, 4827, 5361
Bbv12I GWGCWC 4 cut(s) 163, 710, 1096, 4704
BbvCI CCTCAGC 1 cut(s) 18
BceAI ACGGC 2 cut(s) 47, 1260
BcgI CGANNNNNNTGC 6 cut(s) 549, 583, 2645, 2679, 5222, 5256
BciVI GTATCC 2 cut(s) 1489, 3357
BclI TGATCA 2 cut(s) 700, 1615
BcnI CCSGG 6 cut(s) 1930, 1931, 4071, 4555, 5520, 6020
BfmI CTRYAG 4 cut(s) 78, 1989, 2523, 4339
BfoI RGCGCY 1 cut(s) 733
BfrI CTTAAG 1 cut(s) 2248
BfuAI ACCTGC 4 cut(s) 277, 361, 3032, 3688
BfuI GTATCC 2 cut(s) 1489, 3357
BglI GCCNNNNNGGC 2 cut(s) 3089, 4782
BmcAI AGTACT 1 cut(s) 2056
Bme18I GGWCC 6 cut(s) 599, 1775, 2198, 4328, 4433, 5412
BmeRI GACNNNNNGTC 1 cut(s) 3348
BmeT110I CYCGRG 2 cut(s) 1929, 3487
BmiI GGNNCC 8 cut(s) 1636, 1792, 4069, 4346, 4434, 4775, 5073, 5948
BmrI ACTGGG 4 cut(s) 2474, 2479, 4620, 5971
BmtI GCTAGC 1 cut(s) 5646
BmuI ACTGGG 4 cut(s) 2474, 2479, 4620, 5971
BpiI GAAGAC 5 cut(s) 316, 1434, 1727, 4827, 5361
BplI GAGNNNNNCTC 4 cut(s) 1346, 1378, 2398, 2430
BpmI CTGGAG 9 cut(s) 2119, 2337, 2379, 2628, 3606, 3915, 4027, 4800, 5796
Bpu10I CCTNAGC 3 cut(s) 18, 971, 1753
Bpu14I TTCGAA 1 cut(s) 5099
BpuEI CTTGAG 2 cut(s) 1648, 4688
BpuMI CCSGG 6 cut(s) 1930, 1931, 4071, 4555, 5520, 6020
Bsa29I ATCGAT 1 cut(s) 5493
BsaAI YACGTR 2 cut(s) 1166, 5807
BsaBI GATNNNNATC 1 cut(s) 4989
BsaHI GRCGYC 1 cut(s) 34
BsaI GGTCTC 3 cut(s) 301, 3882, 5929
BsaWI WCCGGW 4 cut(s) 715, 772, 5074, 5122
BsaXI ACNNNNNCTCC 8 cut(s) 810, 840, 1564, 1594, 2310, 2340, 4867, 4897
Bse118I RCCGGY 1 cut(s) 5743
Bse21I CCTNAGG 1 cut(s) 177
Bse3DI GCAATG 4 cut(s) 1735, 2549, 5653, 5931
Bse8I GATNNNNATC 1 cut(s) 4989
BseAI TCCGGA 2 cut(s) 5074, 5122
BseCI ATCGAT 1 cut(s) 5493
BseJI GATNNNNATC 1 cut(s) 4989
BseMI GCAATG 4 cut(s) 1735, 2549, 5653, 5931
BseRI GAGGAG 7 cut(s) 35, 165, 1082, 2625, 3473, 5057, 5879
BseSI GKGCMC 5 cut(s) 163, 1096, 4399, 4778, 5949
BseYI CCCAGC 4 cut(s) 2044, 2530, 4110, 4280
BsgI GTGCAG 6 cut(s) 457, 584, 1287, 3834, 4083, 5462
Bsh1236I CGCG 1 cut(s) 3888
BshNI GGYRCC 3 cut(s) 1790, 4773, 5946
BshVI ATCGAT 1 cut(s) 5493
BsiHKAI GWGCWC 4 cut(s) 163, 710, 1096, 4704
BsiHKCI CYCGRG 2 cut(s) 1929, 3487
BslFI GGGAC 6 cut(s) 41, 3683, 4102, 4217, 4446, 5784
BsmFI GGGAC 6 cut(s) 41, 3683, 4102, 4217, 4446, 5784
BsmI GAATGC 5 cut(s) 386, 1521, 2266, 2857, 3487
Bso31I GGTCTC 3 cut(s) 301, 3882, 5929
BsoBI CYCGRG 2 cut(s) 1929, 3487
Bsp119I TTCGAA 1 cut(s) 5099
Bsp1286I GDGCHC 7 cut(s) 163, 710, 1096, 4399, 4704, 4778, 5949
Bsp13I TCCGGA 2 cut(s) 5074, 5122
Bsp1407I TGTACA 1 cut(s) 964
Bsp19I CCATGG 1 cut(s) 3231
BspDI ATCGAT 1 cut(s) 5493
BspEI TCCGGA 2 cut(s) 5074, 5122
BspFNI CGCG 1 cut(s) 3888
BspHI TCATGA 3 cut(s) 1855, 2824, 3880
BspLI GGNNCC 8 cut(s) 1636, 1792, 4069, 4346, 4434, 4775, 5073, 5948
BspMAI CTGCAG 2 cut(s) 1993, 2527
BspMI ACCTGC 4 cut(s) 277, 361, 3032, 3688
BspOI GCTAGC 1 cut(s) 5646
BspQI GCTCTTC 2 cut(s) 1426, 1711
BspT104I TTCGAA 1 cut(s) 5099
BspT107I GGYRCC 3 cut(s) 1790, 4773, 5946
BspTI CTTAAG 1 cut(s) 2248
BspTNI GGTCTC 3 cut(s) 301, 3882, 5929
BsrBI CCGCTC 1 cut(s) 3463
BsrDI GCAATG 4 cut(s) 1735, 2549, 5653, 5931
BsrFI RCCGGY 1 cut(s) 5743
BsrGI TGTACA 1 cut(s) 964
BssAI RCCGGY 1 cut(s) 5743
BssNI GRCGYC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 3375
BssT1I CCWWGG 5 cut(s) 952, 2022, 2715, 3231, 5220
Bst2BI CACGAG 1 cut(s) 3375
Bst6I CTCTTC 6 cut(s) 100, 436, 1426, 1711, 3140, 5304
BstACI GRCGYC 1 cut(s) 34
BstAFI CTTAAG 1 cut(s) 2248
BstAUI TGTACA 1 cut(s) 964
BstBAI YACGTR 2 cut(s) 1166, 5807
BstBI TTCGAA 1 cut(s) 5099
BstDSI CCRYGG 2 cut(s) 1276, 3231
BstEII GGTNACC 1 cut(s) 4313
BstENI CCTNNNNNAGG 4 cut(s) 195, 3684, 4643, 5728
BstFNI CGCG 1 cut(s) 3888
BstH2I RGCGCY 1 cut(s) 733
BstHHI GCGC 3 cut(s) 732, 2780, 2848
BstNSI RCATGY 3 cut(s) 49, 3857, 6013
BstPI GGTNACC 1 cut(s) 4313
BstSFI CTRYAG 4 cut(s) 78, 1989, 2523, 4339
BstSLI GKGCMC 5 cut(s) 163, 1096, 4399, 4778, 5949
BstSNI TACGTA 1 cut(s) 1166
BstUI CGCG 1 cut(s) 3888
BstV2I GAAGAC 5 cut(s) 316, 1434, 1727, 4827, 5361
BstX2I RGATCY 3 cut(s) 1957, 3580, 4799
BstYI RGATCY 3 cut(s) 1957, 3580, 4799
Bsu15I ATCGAT 1 cut(s) 5493
Bsu36I CCTNAGG 1 cut(s) 177
BsuI GTATCC 2 cut(s) 1489, 3357
BsuTUI ATCGAT 1 cut(s) 5493
BtgI CCRYGG 2 cut(s) 1276, 3231
BtsI GCAGTG 3 cut(s) 567, 2304, 4132
BveI ACCTGC 4 cut(s) 277, 361, 3032, 3688
CaiI CAGNNNCTG 4 cut(s) 308, 935, 3500, 3668
CciI TCATGA 3 cut(s) 1855, 2824, 3880
CfoI GCGC 3 cut(s) 732, 2780, 2848
Cfr10I RCCGGY 1 cut(s) 5743
Cfr9I CCCGGG 1 cut(s) 1929
ClaI ATCGAT 1 cut(s) 5493
CseI GACGC 3 cut(s) 23, 144, 3299
CsiI ACCWGGT 1 cut(s) 4435
CspCI CAANNNNNGTGG 2 cut(s) 1611, 1646
DraI TTTAAA 2 cut(s) 4795, 5680
DriI GACNNNNNGTC 1 cut(s) 3348
EaeI YGGCCR 3 cut(s) 760, 901, 1915
Eam1104I CTCTTC 6 cut(s) 100, 436, 1426, 1711, 3140, 5304
Eam1105I GACNNNNNGTC 1 cut(s) 3348
EarI CTCTTC 6 cut(s) 100, 436, 1426, 1711, 3140, 5304
Ecl136II GAGCTC 2 cut(s) 708, 4702
Eco105I TACGTA 1 cut(s) 1166
Eco130I CCWWGG 5 cut(s) 952, 2022, 2715, 3231, 5220
Eco24I GRGCYC 2 cut(s) 710, 4704
Eco31I GGTCTC 3 cut(s) 301, 3882, 5929
Eco32I GATATC 2 cut(s) 423, 3940
Eco47I GGWCC 6 cut(s) 599, 1775, 2198, 4328, 4433, 5412
Eco47III AGCGCT 1 cut(s) 731
Eco53kI GAGCTC 2 cut(s) 708, 4702
Eco81I CCTNAGG 1 cut(s) 177
Eco88I CYCGRG 2 cut(s) 1929, 3487
Eco91I GGTNACC 1 cut(s) 4313
EcoICRI GAGCTC 2 cut(s) 708, 4702
EcoNI CCTNNNNNAGG 4 cut(s) 195, 3684, 4643, 5728
EcoO109I RGGNCCY 2 cut(s) 4345, 4537
EcoO65I GGTNACC 1 cut(s) 4313
EcoRI GAATTC 4 cut(s) 2410, 3120, 3311, 5101
EcoRV GATATC 2 cut(s) 423, 3940
EcoT14I CCWWGG 5 cut(s) 952, 2022, 2715, 3231, 5220
EcoT22I ATGCAT 2 cut(s) 6, 555
EcoT38I GRGCYC 2 cut(s) 710, 4704
ErhI CCWWGG 5 cut(s) 952, 2022, 2715, 3231, 5220
FaqI GGGAC 6 cut(s) 41, 3683, 4102, 4217, 4446, 5784
FauI CCCGC 2 cut(s) 2547, 5705
FauNDI CATATG 3 cut(s) 2236, 2746, 4372
FbaI TGATCA 2 cut(s) 700, 1615
FblI GTMKAC 1 cut(s) 828
FriOI GRGCYC 2 cut(s) 710, 4704
GlaI GCGC 3 cut(s) 731, 2779, 2847
GsaI CCCAGC 4 cut(s) 2048, 2534, 4114, 4284
GsuI CTGGAG 9 cut(s) 2119, 2337, 2379, 2628, 3606, 3915, 4027, 4800, 5796
HaeII RGCGCY 1 cut(s) 733
HgaI GACGC 3 cut(s) 23, 144, 3299
HhaI GCGC 3 cut(s) 732, 2780, 2848
Hin1I GRCGYC 1 cut(s) 34
Hin6I GCGC 3 cut(s) 730, 2778, 2846
HinP1I GCGC 3 cut(s) 730, 2778, 2846
HincII GTYRAC 5 cut(s) 480, 1213, 1461, 1873, 2492
HindII GTYRAC 5 cut(s) 480, 1213, 1461, 1873, 2492
HindIII AAGCTT 1 cut(s) 281
HpaI GTTAAC 1 cut(s) 480
Hpy99I CGWCG 2 cut(s) 39, 42
HpyCH4IV ACGT 5 cut(s) 724, 1165, 4684, 5289, 5806
HpySE526I ACGT 5 cut(s) 724, 1165, 4684, 5289, 5806
Hsp92I GRCGYC 1 cut(s) 34
HspAI GCGC 3 cut(s) 730, 2778, 2846
Kpn2I TCCGGA 2 cut(s) 5074, 5122
KpnI GGTACC 1 cut(s) 1794
Ksp22I TGATCA 2 cut(s) 700, 1615
KspAI GTTAAC 1 cut(s) 480
LguI GCTCTTC 2 cut(s) 1426, 1711
MabI ACCWGGT 1 cut(s) 4435
MaeII ACGT 5 cut(s) 724, 1165, 4684, 5289, 5806
MbiI CCGCTC 1 cut(s) 3463
MfeI CAATTG 1 cut(s) 5692
MflI RGATCY 3 cut(s) 1957, 3580, 4799
MhlI GDGCHC 7 cut(s) 163, 710, 1096, 4399, 4704, 4778, 5949
MlsI TGGCCA 1 cut(s) 762
MluNI TGGCCA 1 cut(s) 762
MmeI TCCRAC 6 cut(s) 90, 111, 130, 880, 3411, 4779
Mox20I TGGCCA 1 cut(s) 762
Mph1103I ATGCAT 2 cut(s) 6, 555
MroI TCCGGA 2 cut(s) 5074, 5122
MroXI GAANNNNTTC 2 cut(s) 999, 5138
MscI TGGCCA 1 cut(s) 762
MslI CAYNNNNRTG 4 cut(s) 342, 433, 2392, 5399
Msp20I TGGCCA 1 cut(s) 762
MspA1I CMGCKG 2 cut(s) 17, 2633
MspCI CTTAAG 1 cut(s) 2248
MunI CAATTG 1 cut(s) 5692
Mva1269I GAATGC 5 cut(s) 386, 1521, 2266, 2857, 3487
MvnI CGCG 1 cut(s) 3888
NciI CCSGG 6 cut(s) 1930, 1931, 4071, 4555, 5520, 6020
NcoI CCATGG 1 cut(s) 3231
NdeI CATATG 3 cut(s) 2236, 2746, 4372
NheI GCTAGC 1 cut(s) 5642
NlaIV GGNNCC 8 cut(s) 1636, 1792, 4069, 4346, 4434, 4775, 5073, 5948
NsiI ATGCAT 2 cut(s) 6, 555
NspI RCATGY 3 cut(s) 49, 3857, 6013
NspV TTCGAA 1 cut(s) 5099
PacI TTAATTAA 1 cut(s) 738
PaeI GCATGC 1 cut(s) 6013
PagI TCATGA 3 cut(s) 1855, 2824, 3880
PaqCI CACCTGC 1 cut(s) 3688
PciI ACATGT 2 cut(s) 45, 3853
PciSI GCTCTTC 2 cut(s) 1426, 1711
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 5111
PctI GAATGC 5 cut(s) 386, 1521, 2266, 2857, 3487
PdmI GAANNNNTTC 2 cut(s) 999, 5138
PfoI TCCNGGA 3 cut(s) 2200, 3583, 4204
Ppu21I YACGTR 2 cut(s) 1166, 5807
PscI ACATGT 2 cut(s) 45, 3853
PshBI ATTAAT 2 cut(s) 498, 4035
Psp124BI GAGCTC 2 cut(s) 710, 4704
PspEI GGTNACC 1 cut(s) 4313
PspFI CCCAGC 4 cut(s) 2044, 2530, 4110, 4280
PspN4I GGNNCC 8 cut(s) 1636, 1792, 4069, 4346, 4434, 4775, 5073, 5948
PstI CTGCAG 2 cut(s) 1993, 2527
PstNI CAGNNNCTG 4 cut(s) 308, 935, 3500, 3668
PsuI RGATCY 3 cut(s) 1957, 3580, 4799
PvuII CAGCTG 2 cut(s) 17, 2633
RseI CAYNNNNRTG 4 cut(s) 342, 433, 2392, 5399
SacI GAGCTC 2 cut(s) 710, 4704
SapI GCTCTTC 2 cut(s) 1426, 1711
ScaI AGTACT 1 cut(s) 2056
SduI GDGCHC 7 cut(s) 163, 710, 1096, 4399, 4704, 4778, 5949
SexAI ACCWGGT 1 cut(s) 4435
SfcI CTRYAG 4 cut(s) 78, 1989, 2523, 4339
SfuI TTCGAA 1 cut(s) 5099
SinI GGWCC 6 cut(s) 599, 1775, 2198, 4328, 4433, 5412
SmaI CCCGGG 1 cut(s) 1931
SmiI ATTTAAAT 1 cut(s) 5680
SmiMI CAYNNNNRTG 4 cut(s) 342, 433, 2392, 5399
SmlI CTYRAG 3 cut(s) 1663, 2248, 4703
SmoI CTYRAG 3 cut(s) 1663, 2248, 4703
SnaBI TACGTA 1 cut(s) 1166
SphI GCATGC 1 cut(s) 6013
SspI AATATT 3 cut(s) 909, 3079, 5626
SstI GAGCTC 2 cut(s) 710, 4704
StyI CCWWGG 5 cut(s) 952, 2022, 2715, 3231, 5220
SwaI ATTTAAAT 1 cut(s) 5680
TaiI ACGT 5 cut(s) 727, 1168, 4687, 5292, 5809
TatI WGTACW 5 cut(s) 964, 2054, 4350, 4565, 4863
TauI GCSGC 8 cut(s) 1704, 1920, 2357, 2896, 3466, 3780, 4750, 5432
TspGWI ACGGA 6 cut(s) 363, 591, 1378, 1585, 2569, 4594
TspMI CCCGGG 1 cut(s) 1929
Vha464I CTTAAG 1 cut(s) 2248
VneI GTGCAC 2 cut(s) 159, 1092
VpaK11BI GGWCC 6 cut(s) 599, 1775, 2198, 4328, 4433, 5412
VspI ATTAAT 2 cut(s) 498, 4035
XagI CCTNNNNNAGG 4 cut(s) 195, 3684, 4643, 5728
XceI RCATGY 3 cut(s) 49, 3857, 6013
XmaI CCCGGG 1 cut(s) 1929
XmiI GTMKAC 1 cut(s) 828
XmnI GAANNNNTTC 2 cut(s) 999, 5138
ZrmI AGTACT 1 cut(s) 2056
Zsp2I ATGCAT 2 cut(s) 6, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.