pycom111g03240

Paired amphipathic helix protein Sin3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0019127
Physical Location & Seq
Reverse (-)
1956873 .. 1958470
1598 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGGCTAATGGTTTTGAAAGCAATGGGCGATCCCGATTACCTGGCGGCGGAGGAGGCGGTGATTCTCAGAAATTGACAACCGCCGATGCTCTTTTGTTTCTCAAACAGGTGAAGGATGCCTTTTATGACAAAGAAGAAAATTATGAGAAATTTCTCGAGATCATGAAAGATTTCAAGGCTAAAAGGGCTGTCACAGAAGATGTGGTTGCACGAGTGAAGGAATTATTCAAAGGGCATAAGGATTTAATTTTGGGATTTAATCCCTTCTTACCGGAGGGTTATCAGATAACCGCGGATGATGACGAAGGTGCAACTTCGCTGAAACAGACTGTTGATGAAGATGAAGAAGCTGCTGACTCTATAAACTTGACAAACGAGGACAACGGGGAGTGCAGTATCTGCTTGGGGAGACTGAGTGCTGGGTACATTTCACTGGATTGCTCCCACCGCTTCCATGCTAAATGTTTTAGGACCTGGTGGAGCAATAACCCGACGTGCCCATATTGCAGACTTCGGGTCAACGAGAATGGAGAGAAGGATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04139 ko04919 ko05016 ko05202 map04139 map04919 map05016 map05202
Pfam Domains
Protein Families
PAH Sin3_corepress Sin3a_C

Protein Analysis

182

Amino Acids

20.2

Weight (kDa)

4.9

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAH PF02671 46 - 90 5e-17 Paired amphipathic helix repeat
zf-C3HC4_3 PF13920 128 - 173 6.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 130 - 170 2.6e-11 Ring finger domain
zf-C3HC4_2 PF13923 130 - 169 4.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 130 - 155 2.2e-06 RING-like zinc finger
zf-C3HC4 PF00097 131 - 169 1.3e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15025
malus_domestica MD09G1041400.v1.1 MD15G1355900.v1.1
prunus_persica Prupe.3G276200_v2.0.a1 Prupe.3G276200_v2.0.a1
pyrus_communis pycom111g03240
rosa_laevigata RLG00000021909
rosa_samantha Rh2AG616300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 293
AciI CCGC 7 cut(s) 45, 48, 57, 81, 291, 293, 448
AclWI GGATC 1 cut(s) 24
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 3 cut(s) 17, 175, 229
AhdI GACNNNNNGTC 1 cut(s) 515
AjiI CACGTC 1 cut(s) 495
AjnI CCWGG 2 cut(s) 40, 473
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw26I GTCTC 1 cut(s) 403
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 399
Ama87I CYCGRG 1 cut(s) 155
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 170
AspS9I GGNCC 1 cut(s) 471
AsuHPI GGTGA 2 cut(s) 71, 121
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 1 cut(s) 471
BaeGI GKGCMC 1 cut(s) 500
BauI CACGAG 1 cut(s) 210
BbvI GCAGC 1 cut(s) 337
BciT130I CCWGG 2 cut(s) 42, 475
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 1 cut(s) 544
BisI GCNGC 2 cut(s) 46, 351
BlsI GCNGC 2 cut(s) 47, 352
Bme1390I CCNGG 2 cut(s) 42, 475
Bme18I GGWCC 1 cut(s) 471
BmeRI GACNNNNNGTC 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 471
BmrFI CCNGG 2 cut(s) 42, 475
BmsI GCATC 3 cut(s) 76, 106, 529
BsaJI CCNNGG 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 28
BseBI CCWGG 2 cut(s) 42, 475
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 121, 301, 544
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 80, 404
BseNI ACTGG 1 cut(s) 438
BseRI GAGGAG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 500
BseXI GCAGC 1 cut(s) 337
BseYI CCCAGC 1 cut(s) 419
BsgI GTGCAG 1 cut(s) 412
Bsh1236I CGCG 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 500
Bsp143I GATC 2 cut(s) 29, 159
BspACI CCGC 7 cut(s) 45, 48, 57, 81, 291, 293, 448
BspCNI CTCAG 2 cut(s) 79, 405
BspFNI CGCG 1 cut(s) 293
BspHI TCATGA 1 cut(s) 162
BspPI GGATC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 28
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 2 cut(s) 29, 159
BssSI CACGAG 1 cut(s) 210
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 42, 475
Bst4CI ACNGT 1 cut(s) 331
BstAPI GCANNNNNTGC 1 cut(s) 399
BstDEI CTNAG 2 cut(s) 66, 413
BstDSI CCRYGG 1 cut(s) 291
BstF5I GGATG 3 cut(s) 121, 301, 544
BstFNI CGCG 1 cut(s) 293
BstKTI GATC 2 cut(s) 32, 162
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 2 cut(s) 29, 159
BstMWI GCNNNNNNNGC 5 cut(s) 54, 185, 399, 447, 504
BstNI CCWGG 2 cut(s) 42, 475
BstSCI CCNGG 2 cut(s) 40, 473
BstSLI GKGCMC 1 cut(s) 500
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 291
BtrI CACGTC 1 cut(s) 495
BtsCI GGATG 3 cut(s) 121, 301, 544
BtsIMutI CAGTG 1 cut(s) 431
CaiI CAGNNNCTG 1 cut(s) 399
CciI TCATGA 1 cut(s) 162
Cfr13I GGNCC 1 cut(s) 471
Cfr42I CCGCGG 1 cut(s) 294
CsiI ACCWGGT 1 cut(s) 473
Csp6I GTAC 1 cut(s) 424
CviAII CATG 2 cut(s) 163, 455
CviJI RGCY 4 cut(s) 5, 179, 188, 350
CviKI_1 RGCY 4 cut(s) 5, 179, 188, 350
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 2 cut(s) 66, 413
DpnI GATC 2 cut(s) 31, 161
DpnII GATC 2 cut(s) 29, 159
DriI GACNNNNNGTC 1 cut(s) 515
Eam1105I GACNNNNNGTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 40, 473
FaeI CATG 2 cut(s) 166, 458
FaiI YATR 7 cut(s) 126, 144, 164, 237, 362, 456, 502
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FatI CATG 2 cut(s) 162, 454
Fnu4HI GCNGC 2 cut(s) 46, 351
FokI GGATG 2 cut(s) 128, 308
Fsp4HI GCNGC 2 cut(s) 46, 351
FspBI CTAG 1 cut(s) 544
GluI GCNGC 2 cut(s) 46, 351
GsaI CCCAGC 1 cut(s) 423
HapII CCGG 1 cut(s) 272
Hin1II CATG 2 cut(s) 166, 458
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 2 cut(s) 62, 356
HpaII CCGG 1 cut(s) 272
HphI GGTGA 2 cut(s) 71, 121
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 2 cut(s) 69, 285
Hpy188III TCNNGA 4 cut(s) 33, 155, 157, 163
Hpy8I GTNNAC 1 cut(s) 520
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 5 cut(s) 106, 211, 274, 299, 529
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 4 cut(s) 209, 311, 393, 507
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 185, 399, 447, 504
HpyF3I CTNAG 2 cut(s) 66, 413
HpySE526I ACGT 1 cut(s) 494
Hsp92II CATG 2 cut(s) 166, 458
KspI CCGCGG 1 cut(s) 294
Kzo9I GATC 2 cut(s) 29, 159
LmnI GCTCC 2 cut(s) 446, 480
LpnPI CCDG 8 cut(s) 27, 54, 92, 285, 405, 419, 460, 487
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 3 cut(s) 76, 106, 529
MabI ACCWGGT 1 cut(s) 473
MaeI CTAG 1 cut(s) 544
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 1 cut(s) 190
MalI GATC 2 cut(s) 31, 161
MboI GATC 2 cut(s) 29, 159
MboII GAAGA 4 cut(s) 146, 209, 350, 356
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 5 cut(s) 71, 139, 149, 221, 246
MlyI GAGTC 1 cut(s) 350
MnlI CCTC 4 cut(s) 44, 47, 268, 370
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 2 cut(s) 245, 258
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 2 cut(s) 42, 475
MvaI CCWGG 2 cut(s) 42, 475
MvnI CGCG 1 cut(s) 293
MwoI GCNNNNNNNGC 5 cut(s) 54, 185, 399, 447, 504
NdeII GATC 2 cut(s) 29, 159
NlaIII CATG 2 cut(s) 166, 458
NmuCI GTSAC 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 155
PagI TCATGA 1 cut(s) 162
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 47, 352
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
PpuMI RGGWCCY 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 40, 473
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 2 cut(s) 40, 473
PspPI GGNCC 1 cut(s) 471
PspPPI RGGWCCY 1 cut(s) 471
PstNI CAGNNNCTG 1 cut(s) 399
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
SacII CCGCGG 1 cut(s) 294
SaqAI TTAA 2 cut(s) 245, 258
SatI GCNGC 2 cut(s) 46, 351
Sau3AI GATC 2 cut(s) 29, 159
Sau96I GGNCC 1 cut(s) 471
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 2 cut(s) 42, 475
SduI GDGCHC 1 cut(s) 500
SetI ASST 6 cut(s) 43, 111, 310, 352, 476, 497
SexAI ACCWGGT 1 cut(s) 473
SfaNI GCATC 3 cut(s) 76, 106, 529
Sfr274I CTCGAG 1 cut(s) 155
Sfr303I CCGCGG 1 cut(s) 294
SgrBI CCGCGG 1 cut(s) 294
SinI GGWCC 1 cut(s) 471
SlaI CTCGAG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 5 cut(s) 71, 139, 149, 221, 246
SsiI CCGC 7 cut(s) 45, 48, 57, 81, 291, 293, 448
SspMI CTAG 1 cut(s) 544
StyD4I CCNGG 2 cut(s) 40, 473
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 1 cut(s) 497
TaqI TCGA 1 cut(s) 156
TasI AATT 5 cut(s) 71, 139, 149, 221, 246
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 1 cut(s) 62
Tru1I TTAA 2 cut(s) 245, 258
Tru9I TTAA 2 cut(s) 245, 258
TscAI CASTG 1 cut(s) 438
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 179, 351, 357
TspRI CASTG 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.