pycom11g07670

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0022551
Physical Location & Seq
Reverse (-)
5881962 .. 5882743
782 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g07670.1

Sequence Viewer

Length: 690 bp
ATGATAATACATGTACCTTTATATATTCACAAGCTCATTGGAGCTCGATTTTACACAGAAGACTCTGCAACGGATGCATCAGGCCATGGAACCCACACTGCCTCAACGGCAGCTGGGAATGCTGTAAAGGACGTAAGCTTTTATGGGCTAGCACAAGGGACTGCAAGAGGAGGGGTTCCTGCTGCAAGAATTGCAGCATATAAAGTCTGCACTGCTCGAGCATGTAAGGCACACGATATTTTGGCTGCTTTTGACGATGCTATTGCTGATGGAGTCGACAGCATTACAATTTCTATTTCAGTTGGTTCTGTACAAGAGTTTTATGGGGATCCTGTTGCCATTGGTGCTTTCCATGCAAAGGAGAAAGGGATACTAACCGCAAACTCTGCAGGCAACACTGGTCCTTCAGCTAATACTGTATCAAGTCTGGCTCCATGGATACTGACAGTCGCAGCAAGTAGCATAGATCGATGGATCATCGACGAGGTTGTTTTTGGAAATGGAACAACAGTACTCGTGTCATCAATTTCCCTCATCCAATACAATATAATGTCGGGAACCTCCATGTCTTGCCCACACAATGCTGGTGTAGCTGCTTATGTTAAAAGCTTCCACCCTGATTGGTCTCCGGCAGCCATCAAATCATCTCTTATGACTACCGGTATTTACTATTTCTTGATTAATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

230

Amino Acids

23.85

Weight (kDa)

6.27

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 19 - 220 1e-23 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022551)

Species Orthologous Gene IDs
pyrus_communis pycom11g07670
rosa_chinensis RchiOBHm_Chr6g0306571
rosa_roxburghii Rroxscaffold_7G00161660
rosa_samantha Rh6CG477100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 276
AciI CCGC 1 cut(s) 378
AclWI GGATC 3 cut(s) 323, 336, 482
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 3 cut(s) 15, 312, 513
AfiI CCNNNNNNNGG 1 cut(s) 358
AflIII ACRYGT 1 cut(s) 10
AgeI ACCGGT 1 cut(s) 659
AluBI AGCT 7 cut(s) 34, 44, 113, 138, 410, 593, 609
AluI AGCT 7 cut(s) 34, 44, 113, 138, 410, 593, 609
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 630
AlwI GGATC 3 cut(s) 323, 336, 482
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 7 cut(s) 110, 182, 194, 245, 452, 593, 632
AseI ATTAAT 1 cut(s) 681
AsiGI ACCGGT 1 cut(s) 659
AspS9I GGNCC 1 cut(s) 401
AsuNHI GCTAGC 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 216
AvaII GGWCC 1 cut(s) 401
BamHI GGATCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 46
BauI CACGAG 1 cut(s) 515
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 7 cut(s) 122, 169, 206, 232, 464, 580, 644
BccI CCATC 3 cut(s) 263, 465, 644
BceAI ACGGC 1 cut(s) 123
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciVI GTATCC 2 cut(s) 363, 432
BcoDI GTCTC 1 cut(s) 630
BfaI CTAG 1 cut(s) 149
BfmI CTRYAG 1 cut(s) 387
BfuI GTATCC 2 cut(s) 363, 432
BglI GCCNNNNNGGC 1 cut(s) 107
BisI GCNGC 7 cut(s) 111, 183, 195, 246, 453, 594, 633
BlsI GCNGC 7 cut(s) 112, 184, 196, 247, 454, 595, 634
BmcAI AGTACT 1 cut(s) 513
Bme18I GGWCC 1 cut(s) 401
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 5 cut(s) 91, 177, 330, 432, 559
BmsI GCATC 3 cut(s) 64, 86, 247
BmtI GCTAGC 1 cut(s) 152
BpiI GAAGAC 1 cut(s) 66
Bsa29I ATCGAT 1 cut(s) 469
BsaI GGTCTC 1 cut(s) 630
BsaJI CCNNGG 2 cut(s) 85, 434
BsaWI WCCGGW 1 cut(s) 659
Bsc4I CCNNNNNNNGG 1 cut(s) 358
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 403
BseCI ATCGAT 1 cut(s) 469
BseDI CCNNGG 2 cut(s) 85, 434
BseGI GGATG 2 cut(s) 79, 534
BseLI CCNNNNNNNGG 1 cut(s) 358
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 7 cut(s) 122, 169, 206, 232, 464, 580, 644
BseYI CCCAGC 1 cut(s) 113
BsgI GTGCAG 1 cut(s) 193
BshFI GGCC 1 cut(s) 84
BshTI ACCGGT 1 cut(s) 659
BshVI ATCGAT 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 629, 660
BslFI GGGAC 1 cut(s) 172
BslI CCNNNNNNNGG 1 cut(s) 358
BsmAI GTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 172
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 1 cut(s) 84
Bso31I GGTCTC 1 cut(s) 630
BsoBI CYCGRG 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 310
Bsp143I GATC 3 cut(s) 328, 466, 474
Bsp19I CCATGG 2 cut(s) 85, 434
BspACI CCGC 1 cut(s) 378
BspANI GGCC 1 cut(s) 84
BspDI ATCGAT 1 cut(s) 469
BspLI GGNNCC 5 cut(s) 91, 177, 330, 432, 559
BspMAI CTGCAG 1 cut(s) 391
BspOI GCTAGC 1 cut(s) 152
BspPI GGATC 3 cut(s) 323, 336, 482
BspTNI GGTCTC 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 310
BsrI ACTGG 1 cut(s) 403
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 85, 434
BssMI GATC 3 cut(s) 328, 466, 474
BssSI CACGAG 1 cut(s) 515
BssT1I CCWWGG 2 cut(s) 85, 434
Bst2BI CACGAG 1 cut(s) 515
Bst4CI ACNGT 3 cut(s) 418, 448, 511
BstAPI GCANNNNNTGC 3 cut(s) 74, 191, 386
BstAUI TGTACA 1 cut(s) 310
BstC8I GCNNGC 2 cut(s) 150, 391
BstDSI CCRYGG 2 cut(s) 85, 434
BstF5I GGATG 2 cut(s) 79, 534
BstKTI GATC 3 cut(s) 331, 469, 477
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 3 cut(s) 328, 466, 474
BstMWI GCNNNNNNNGC 9 cut(s) 74, 107, 119, 191, 227, 344, 353, 386, 590
BstNSI RCATGY 2 cut(s) 14, 225
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 7 cut(s) 122, 169, 206, 232, 464, 580, 644
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 469
BsuI GTATCC 2 cut(s) 363, 432
BsuRI GGCC 1 cut(s) 84
BsuTUI ATCGAT 1 cut(s) 469
BtgI CCRYGG 2 cut(s) 85, 434
BtsCI GGATG 2 cut(s) 79, 534
BtsI GCAGTG 2 cut(s) 96, 210
BtsIMutI CAGTG 3 cut(s) 96, 210, 396
Cac8I GCNNGC 2 cut(s) 150, 391
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 1 cut(s) 401
ClaI ATCGAT 1 cut(s) 469
Csp6I GTAC 3 cut(s) 14, 311, 512
CspAI ACCGGT 1 cut(s) 659
CspCI CAANNNNNGTGG 2 cut(s) 602, 637
CviAII CATG 6 cut(s) 11, 86, 222, 353, 435, 565
CviQI GTAC 3 cut(s) 14, 311, 512
DpnI GATC 3 cut(s) 330, 468, 476
DpnII GATC 3 cut(s) 328, 466, 474
Ecl136II GAGCTC 1 cut(s) 44
Eco130I CCWWGG 2 cut(s) 85, 434
Eco24I GRGCYC 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 630
Eco47I GGWCC 1 cut(s) 401
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 44
EcoT14I CCWWGG 2 cut(s) 85, 434
EcoT22I ATGCAT 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 46
ErhI CCWWGG 2 cut(s) 85, 434
FaeI CATG 6 cut(s) 14, 89, 225, 356, 438, 568
FaqI GGGAC 1 cut(s) 172
FatI CATG 6 cut(s) 10, 85, 221, 352, 434, 564
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 7 cut(s) 111, 183, 195, 246, 453, 594, 633
FokI GGATG 2 cut(s) 86, 521
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 7 cut(s) 111, 183, 195, 246, 453, 594, 633
FspBI CTAG 1 cut(s) 149
GluI GCNGC 7 cut(s) 111, 183, 195, 246, 453, 594, 633
GsaI CCCAGC 1 cut(s) 117
HaeIII GGCC 1 cut(s) 84
HapII CCGG 2 cut(s) 629, 660
Hin1II CATG 6 cut(s) 14, 89, 225, 356, 438, 568
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 2 cut(s) 136, 607
HinfI GANTC 2 cut(s) 62, 273
HpaII CCGG 2 cut(s) 629, 660
Hpy166II GTNNAC 1 cut(s) 277
Hpy188III TCNNGA 2 cut(s) 555, 676
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 1 cut(s) 414
HpyCH4III ACNGT 3 cut(s) 418, 448, 511
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 8 cut(s) 68, 77, 164, 185, 194, 210, 356, 389
HpyF10VI GCNNNNNNNGC 9 cut(s) 74, 107, 119, 191, 227, 344, 353, 386, 590
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 6 cut(s) 14, 89, 225, 356, 438, 568
Kzo9I GATC 3 cut(s) 328, 466, 474
LmnI GCTCC 2 cut(s) 41, 436
Lsp1109I GCAGC 7 cut(s) 122, 169, 206, 232, 464, 580, 644
LweI GCATC 3 cut(s) 64, 86, 247
MaeI CTAG 1 cut(s) 149
MaeII ACGT 1 cut(s) 132
MalI GATC 3 cut(s) 330, 468, 476
MboI GATC 3 cut(s) 328, 466, 474
MboII GAAGA 1 cut(s) 71
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 3 cut(s) 189, 288, 525
MlyI GAGTC 2 cut(s) 56, 282
MnlI CCTC 6 cut(s) 112, 161, 164, 478, 542, 571
Mph1103I ATGCAT 1 cut(s) 79
MseI TTAA 2 cut(s) 603, 681
MspA1I CMGCKG 1 cut(s) 113
MspI CCGG 2 cut(s) 629, 660
Mva1269I GAATGC 1 cut(s) 124
MwoI GCNNNNNNNGC 9 cut(s) 74, 107, 119, 191, 227, 344, 353, 386, 590
NcoI CCATGG 2 cut(s) 85, 434
NdeII GATC 3 cut(s) 328, 466, 474
NheI GCTAGC 1 cut(s) 148
NlaIII CATG 6 cut(s) 14, 89, 225, 356, 438, 568
NlaIV GGNNCC 5 cut(s) 91, 177, 330, 432, 559
NsiI ATGCAT 1 cut(s) 79
NspI RCATGY 2 cut(s) 14, 225
PaeR7I CTCGAG 1 cut(s) 216
PciI ACATGT 1 cut(s) 10
PctI GAATGC 1 cut(s) 124
PinAI ACCGGT 1 cut(s) 659
PkrI GCNGC 7 cut(s) 112, 184, 196, 247, 454, 595, 634
PleI GAGTC 2 cut(s) 56, 281
PpsI GAGTC 2 cut(s) 56, 281
PscI ACATGT 1 cut(s) 10
PshBI ATTAAT 1 cut(s) 681
Psp124BI GAGCTC 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 113
PspN4I GGNNCC 5 cut(s) 91, 177, 330, 432, 559
PspPI GGNCC 1 cut(s) 401
PspXI VCTCGAGB 1 cut(s) 216
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 113
RsaI GTAC 3 cut(s) 15, 312, 513
RsaNI GTAC 3 cut(s) 14, 311, 512
SacI GAGCTC 1 cut(s) 46
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 603, 681
SatI GCNGC 7 cut(s) 111, 183, 195, 246, 453, 594, 633
Sau3AI GATC 3 cut(s) 328, 466, 474
Sau96I GGNCC 1 cut(s) 401
ScaI AGTACT 1 cut(s) 513
SchI GAGTC 2 cut(s) 56, 282
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 3 cut(s) 64, 86, 247
SfcI CTRYAG 1 cut(s) 387
Sfr274I CTCGAG 1 cut(s) 216
SinI GGWCC 1 cut(s) 401
SlaI CTCGAG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
Sse9I AATT 3 cut(s) 189, 288, 525
SsiI CCGC 1 cut(s) 378
SspMI CTAG 1 cut(s) 149
SstI GAGCTC 1 cut(s) 46
StyI CCWWGG 2 cut(s) 85, 434
TaaI ACNGT 3 cut(s) 418, 448, 511
TaiI ACGT 1 cut(s) 135
TaqI TCGA 5 cut(s) 46, 217, 276, 469, 480
TasI AATT 3 cut(s) 189, 288, 525
TatI WGTACW 2 cut(s) 310, 511
Tru1I TTAA 2 cut(s) 603, 681
Tru9I TTAA 2 cut(s) 603, 681
TscAI CASTG 3 cut(s) 103, 217, 403
TseI GCWGC 7 cut(s) 110, 182, 194, 245, 452, 593, 632
TspGWI ACGGA 1 cut(s) 86
TspRI CASTG 3 cut(s) 103, 217, 403
VpaK11BI GGWCC 1 cut(s) 401
VspI ATTAAT 1 cut(s) 681
XceI RCATGY 2 cut(s) 14, 225
XhoI CTCGAG 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 513
Zsp2I ATGCAT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.