Rroxscaffold_7G00161660

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0022551
Physical Location & Seq
Reverse (-)
4367335 .. 4367966
632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161660.1

Sequence Viewer

Length: 573 bp
ATGATGGCCTTCTTAACCGGTCTCATTCCGGCTTCGACGTCATTGTCGGTGTGGTCGACACTGGAATATGACCGAAGCACAGCAGCTTCCACAACCACGGCCTGGGACCAATCCCCTCGCGTTGGAAAGATAACTGTGTCCCAGGAGATTGGGAGAATCCGGCAACCTTTTGAAACAGATCGAAAGCTCAGAGGAGCCAGATATATATATACTTCACTCGCGGCAGCAATTTTACTTCCGCAAGAGACCAGGGCGATCGAGGAGGACAGTTCCGGCCACGGCACACACGTGGGCACGTGGCGTCAATCATCGCTGGACGACATGTCCCAAACGCCTCCTTCCTCGGCTTTGCTCAAGGTGTTGCATCAGGAGTTGCTCCAAACGCAAGACTTGACACACGGTTGCGACGTCATATCTTTGCCATTGTCATTAGACGACGGCGACCTCCGCCCTTATCACAAGGACCCGATTGCTGTAGCTGCATTGTCCGCAACGTACCCATGCCAGAAGGGCATCCTTGTTTCGGCCTCCGCAAGCAACGGCGGCCCACGTGAGAAGAGCGTGCATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

190

Amino Acids

20.56

Weight (kDa)

6.04

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022551)

Species Orthologous Gene IDs
pyrus_communis pycom11g07670
rosa_chinensis RchiOBHm_Chr6g0306571
rosa_roxburghii Rroxscaffold_7G00161660
rosa_samantha Rh6CG477100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AatII GACGTC 2 cut(s) 41, 411
AccB7I CCANNNNNTGG 1 cut(s) 102
AccI GTMKAC 1 cut(s) 56
AccII CGCG 2 cut(s) 120, 221
AciI CCGC 6 cut(s) 221, 239, 448, 489, 531, 543
AcoI YGGCCR 1 cut(s) 274
AcvI CACGTG 3 cut(s) 289, 297, 551
AcyI GRCGYC 3 cut(s) 38, 301, 408
AfaI GTAC 1 cut(s) 497
AfiI CCNNNNNNNGG 3 cut(s) 102, 122, 523
AflIII ACRYGT 2 cut(s) 286, 321
AgeI ACCGGT 1 cut(s) 17
AgsI TTSAA 1 cut(s) 173
AhdI GACNNNNNGTC 1 cut(s) 322
AjnI CCWGG 3 cut(s) 101, 141, 248
AleI CACNNNNGTG 1 cut(s) 287
AluBI AGCT 3 cut(s) 86, 187, 479
AluI AGCT 3 cut(s) 86, 187, 479
Alw26I GTCTC 2 cut(s) 26, 239
AoxI GGCC 5 cut(s) 6, 99, 274, 525, 544
ApeKI GCWGC 3 cut(s) 83, 224, 479
AsiGI ACCGGT 1 cut(s) 17
AspS9I GGNCC 3 cut(s) 106, 463, 545
AvaII GGWCC 2 cut(s) 106, 463
BaeGI GKGCMC 1 cut(s) 296
BbrPI CACGTG 3 cut(s) 289, 297, 551
BbvI GCAGC 3 cut(s) 95, 236, 466
BceAI ACGGC 4 cut(s) 114, 295, 454, 556
BciT130I CCWGG 3 cut(s) 103, 143, 250
BcoDI GTCTC 2 cut(s) 26, 239
BfmI CTRYAG 1 cut(s) 474
BglI GCCNNNNNGGC 1 cut(s) 510
BisI GCNGC 5 cut(s) 84, 222, 225, 480, 544
BlsI GCNGC 5 cut(s) 85, 223, 226, 481, 545
Bme1390I CCNGG 3 cut(s) 103, 143, 250
Bme18I GGWCC 2 cut(s) 106, 463
BmeRI GACNNNNNGTC 1 cut(s) 322
BmgT120I GGNCC 3 cut(s) 106, 463, 545
BmiI GGNNCC 3 cut(s) 107, 196, 465
BmrFI CCNGG 3 cut(s) 103, 143, 250
BmsI GCATC 2 cut(s) 373, 522
BpuEI CTTGAG 1 cut(s) 338
BsaAI YACGTR 3 cut(s) 289, 297, 551
BsaHI GRCGYC 3 cut(s) 38, 301, 408
BsaI GGTCTC 2 cut(s) 26, 239
BsaJI CCNNGG 6 cut(s) 96, 102, 141, 249, 277, 342
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 122, 523
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 3 cut(s) 103, 143, 250
BseDI CCNNGG 6 cut(s) 96, 102, 141, 249, 277, 342
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 3 cut(s) 102, 122, 523
BseMII CTCAG 1 cut(s) 202
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 2 cut(s) 207, 275
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 3 cut(s) 95, 236, 466
Bsh1236I CGCG 2 cut(s) 120, 221
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 5 cut(s) 8, 101, 276, 527, 546
BshTI ACCGGT 1 cut(s) 17
BsiEI CGRYCG 1 cut(s) 258
BsiSI CCGG 4 cut(s) 18, 29, 160, 273
BslFI GGGAC 3 cut(s) 119, 124, 310
BslI CCNNNNNNNGG 3 cut(s) 102, 122, 523
BsmAI GTCTC 2 cut(s) 26, 239
BsmFI GGGAC 3 cut(s) 119, 124, 310
BsnI GGCC 5 cut(s) 8, 101, 276, 527, 546
Bso31I GGTCTC 2 cut(s) 26, 239
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 2 cut(s) 178, 255
BspACI CCGC 6 cut(s) 221, 239, 448, 489, 531, 543
BspANI GGCC 5 cut(s) 8, 101, 276, 527, 546
BspCNI CTCAG 1 cut(s) 201
BspFNI CGCG 2 cut(s) 120, 221
BspLI GGNNCC 3 cut(s) 107, 196, 465
BspQI GCTCTTC 1 cut(s) 551
BspTNI GGTCTC 2 cut(s) 26, 239
BsrFI RCCGGY 1 cut(s) 17
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 6 cut(s) 96, 102, 141, 249, 277, 342
BssMI GATC 2 cut(s) 178, 255
BssNI GRCGYC 3 cut(s) 38, 301, 408
Bst2UI CCWGG 3 cut(s) 103, 143, 250
Bst4CI ACNGT 3 cut(s) 136, 269, 401
Bst6I CTCTTC 1 cut(s) 551
BstACI GRCGYC 3 cut(s) 38, 301, 408
BstBAI YACGTR 3 cut(s) 289, 297, 551
BstC8I GCNNGC 3 cut(s) 535, 563, 567
BstDEI CTNAG 1 cut(s) 188
BstDSI CCRYGG 2 cut(s) 96, 277
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 2 cut(s) 120, 221
BstKTI GATC 2 cut(s) 181, 258
BstMAI GTCTC 2 cut(s) 26, 239
BstMBI GATC 2 cut(s) 178, 255
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 6 cut(s) 382, 447, 479, 488, 510, 543
BstNI CCWGG 3 cut(s) 103, 143, 250
BstNSI RCATGY 2 cut(s) 325, 569
BstSCI CCNGG 3 cut(s) 101, 141, 248
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 2 cut(s) 120, 221
BstV1I GCAGC 3 cut(s) 95, 236, 466
BstXI CCANNNNNNTGG 1 cut(s) 149
BsuRI GGCC 5 cut(s) 8, 101, 276, 527, 546
BtgI CCRYGG 2 cut(s) 96, 277
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 513
BtsIMutI CAGTG 1 cut(s) 59
Cac8I GCNNGC 3 cut(s) 535, 563, 567
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 106, 463, 545
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 496
CspAI ACCGGT 1 cut(s) 17
CviAII CATG 3 cut(s) 322, 501, 566
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 188
DpnI GATC 2 cut(s) 180, 257
DpnII GATC 2 cut(s) 178, 255
DrdI GACNNNNNNGTC 1 cut(s) 43
DriI GACNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 551
Eam1105I GACNNNNNGTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 551
EciI GGCGGA 1 cut(s) 437
Eco31I GGTCTC 2 cut(s) 26, 239
Eco47I GGWCC 2 cut(s) 106, 463
Eco72I CACGTG 3 cut(s) 289, 297, 551
EcoO109I RGGNCCY 1 cut(s) 463
EcoRII CCWGG 3 cut(s) 101, 141, 248
FaeI CATG 3 cut(s) 325, 504, 569
FaiI YATR 9 cut(s) 69, 204, 206, 208, 210, 323, 413, 502, 567
FaqI GGGAC 3 cut(s) 119, 124, 310
FatI CATG 3 cut(s) 321, 500, 565
FblI GTMKAC 1 cut(s) 56
Fnu4HI GCNGC 5 cut(s) 84, 222, 225, 480, 544
FokI GGATG 1 cut(s) 500
Fsp4HI GCNGC 5 cut(s) 84, 222, 225, 480, 544
GluI GCNGC 5 cut(s) 84, 222, 225, 480, 544
HaeIII GGCC 5 cut(s) 8, 101, 276, 527, 546
HapII CCGG 4 cut(s) 18, 29, 160, 273
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 3 cut(s) 38, 301, 408
Hin1II CATG 3 cut(s) 325, 504, 569
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 1 cut(s) 156
HpaII CCGG 4 cut(s) 18, 29, 160, 273
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 1 cut(s) 368
Hpy8I GTNNAC 1 cut(s) 57
Hpy99I CGWCG 3 cut(s) 40, 410, 440
HpyAV CCTTC 3 cut(s) 19, 348, 502
HpyCH4III ACNGT 3 cut(s) 136, 269, 401
HpyCH4IV ACGT 6 cut(s) 38, 288, 296, 408, 494, 550
HpyCH4V TGCA 3 cut(s) 364, 482, 565
HpyF10VI GCNNNNNNNGC 6 cut(s) 382, 447, 479, 488, 510, 543
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 6 cut(s) 38, 288, 296, 408, 494, 550
Hsp92I GRCGYC 3 cut(s) 38, 301, 408
Hsp92II CATG 3 cut(s) 325, 504, 569
Kzo9I GATC 2 cut(s) 178, 255
LguI GCTCTTC 1 cut(s) 551
LmnI GCTCC 2 cut(s) 194, 381
Lsp1109I GCAGC 3 cut(s) 95, 236, 466
LweI GCATC 2 cut(s) 373, 522
MaeII ACGT 6 cut(s) 38, 288, 296, 408, 494, 550
MalI GATC 2 cut(s) 180, 257
MboI GATC 2 cut(s) 178, 255
MboII GAAGA 1 cut(s) 568
MhlI GDGCHC 1 cut(s) 296
MluCI AATT 1 cut(s) 228
MmeI TCCRAC 1 cut(s) 103
MnlI CCTC 8 cut(s) 126, 185, 253, 256, 345, 352, 455, 538
MseI TTAA 2 cut(s) 14, 571
MslI CAYNNNNRTG 1 cut(s) 287
MspI CCGG 4 cut(s) 18, 29, 160, 273
MspR9I CCNGG 3 cut(s) 103, 143, 250
MvaI CCWGG 3 cut(s) 103, 143, 250
MvnI CGCG 2 cut(s) 120, 221
MwoI GCNNNNNNNGC 6 cut(s) 382, 447, 479, 488, 510, 543
NdeII GATC 2 cut(s) 178, 255
NlaIII CATG 3 cut(s) 325, 504, 569
NlaIV GGNNCC 3 cut(s) 107, 196, 465
NmeAIII GCCGAG 1 cut(s) 323
NspI RCATGY 2 cut(s) 325, 569
OliI CACNNNNGTG 1 cut(s) 287
PaeI GCATGC 1 cut(s) 569
PciI ACATGT 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 551
PcsI WCGNNNNNNNCGW 3 cut(s) 53, 285, 405
PfeI GAWTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 17
PkrI GCNGC 5 cut(s) 85, 223, 226, 481, 545
Ple19I CGATCG 1 cut(s) 258
PmaCI CACGTG 3 cut(s) 289, 297, 551
PmlI CACGTG 3 cut(s) 289, 297, 551
Ppu21I YACGTR 3 cut(s) 289, 297, 551
PpuMI RGGWCCY 1 cut(s) 463
PscI ACATGT 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 3 cut(s) 101, 141, 248
PspCI CACGTG 3 cut(s) 289, 297, 551
PspGI CCWGG 3 cut(s) 101, 141, 248
PspN4I GGNNCC 3 cut(s) 107, 196, 465
PspPI GGNCC 3 cut(s) 106, 463, 545
PspPPI RGGWCCY 1 cut(s) 463
PvuI CGATCG 1 cut(s) 258
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 287
SalI GTCGAC 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 551
SaqAI TTAA 2 cut(s) 14, 571
SatI GCNGC 5 cut(s) 84, 222, 225, 480, 544
Sau3AI GATC 2 cut(s) 178, 255
Sau96I GGNCC 3 cut(s) 106, 463, 545
ScrFI CCNGG 3 cut(s) 103, 143, 250
SduI GDGCHC 1 cut(s) 296
SfaNI GCATC 2 cut(s) 373, 522
SfcI CTRYAG 1 cut(s) 474
SinI GGWCC 2 cut(s) 106, 463
SmiMI CAYNNNNRTG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 569
Sse9I AATT 1 cut(s) 228
SsiI CCGC 6 cut(s) 221, 239, 448, 489, 531, 543
StyD4I CCNGG 3 cut(s) 101, 141, 248
TaaI ACNGT 3 cut(s) 136, 269, 401
TaiI ACGT 6 cut(s) 41, 291, 299, 411, 497, 553
TaqI TCGA 4 cut(s) 35, 56, 181, 258
TaqII GACCGA 1 cut(s) 87
TasI AATT 1 cut(s) 228
TauI GCSGC 2 cut(s) 224, 546
TfiI GAWTC 1 cut(s) 156
Tru1I TTAA 2 cut(s) 14, 571
Tru9I TTAA 2 cut(s) 14, 571
TscAI CASTG 1 cut(s) 66
TseI GCWGC 3 cut(s) 83, 224, 479
TspRI CASTG 1 cut(s) 66
Van91I CCANNNNNTGG 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 106, 463
XceI RCATGY 2 cut(s) 325, 569
XmiI GTMKAC 1 cut(s) 56
ZraI GACGTC 2 cut(s) 39, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.