RchiOBHm_Chr6g0306571

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0022551
Physical Location & Seq
Forward (+)
65227543 .. 65229037
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27559

Sequence Viewer

Length: 762 bp
ATGGGACTGCGTTATAGGTATACTCAAGAGATTTTGGATCGAAACTTGGGTTGGGCCAGCCCGTACCCTGCTTCGACGTCATTGTCGGTGTGGTCGACACTGGAATATGGCCGGAGCACAGCAGCTTCCACAACCACGGCCTGGGACCAATCCCCTCGCGTTGGAAAGGTAACTGTGTCCCCGGAGATTCGGAGAATCCGGCAGCCTTTTGCAACAGATCGAAAGCTCATAGGAGCCAGCCAGATACTTGACTCGCGCGCGGCAGCAATTTTACTTCCGCAAGAGACCAGGGCGAGGAGGACAGTTCCGGCCGGCCACGGCACACACGTGGCGTCAATCATCGCTGGACGACATGTCCCAAACGCCTCTTTCCTCGGCTTTGCTCAGGGTGTTGCATCAGGAGTTGCTCCAAACGCAAGAGCTGCCATGTACAGAATTGCGCGCGGCAGCAGGAGAGTGAGTCTAACTTGCTGGCTGCCCGTAGAAGACGGTTGCGACGTCATATCTTTGTCATTGTCATTAGACGACGGCGACCTCCGCCCTTATCACAAGGACCCGATTGATATAGCTGCATTGCTCGGGAACGGTCGGATCCTTACTGGCTCTTCTCTCTACAATGGAACTTGGCCGGAGAACACGTGCATGTCTCTTGTTGCAGGCCTAATCTCCGGCGGCTTCACCGGAAAGGAAACATATCCGTTTGCAATCACGTACATCGACGATAGCAATTGTGAAGAATATCATCGGGATGGTAAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

253

Amino Acids

27.45

Weight (kDa)

8.84

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 104 - 184 5.1e-07 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022551)

Species Orthologous Gene IDs
pyrus_communis pycom11g07670
rosa_chinensis RchiOBHm_Chr6g0306571
rosa_roxburghii Rroxscaffold_7G00161660
rosa_samantha Rh6CG477100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 82
AatII GACGTC 2 cut(s) 80, 501
AccB7I CCANNNNNTGG 1 cut(s) 141
AccI GTMKAC 2 cut(s) 20, 95
AccII CGCG 6 cut(s) 159, 256, 258, 260, 442, 444
AciI CCGC 5 cut(s) 260, 278, 444, 538, 672
AclWI GGATC 3 cut(s) 45, 586, 599
AcoI YGGCCR 4 cut(s) 109, 309, 313, 626
AcvI CACGTG 2 cut(s) 328, 639
AcyI GRCGYC 3 cut(s) 77, 332, 498
AfaI GTAC 3 cut(s) 65, 431, 713
AfiI CCNNNNNNNGG 3 cut(s) 141, 161, 294
AflIII ACRYGT 3 cut(s) 325, 352, 636
AhdI GACNNNNNGTC 1 cut(s) 353
AjnI CCWGG 2 cut(s) 140, 287
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 4 cut(s) 125, 226, 422, 569
AluI AGCT 4 cut(s) 125, 226, 422, 569
Alw21I GWGCWC 1 cut(s) 119
Alw26I GTCTC 2 cut(s) 278, 651
AlwI GGATC 3 cut(s) 45, 586, 599
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 7 cut(s) 54, 109, 138, 309, 313, 626, 658
ApeKI GCWGC 7 cut(s) 122, 202, 263, 422, 447, 475, 569
AspLEI GCGC 4 cut(s) 258, 260, 442, 444
AspS9I GGNCC 3 cut(s) 54, 145, 553
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 670
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 2 cut(s) 145, 553
BamHI GGATCC 1 cut(s) 591
BbrPI CACGTG 2 cut(s) 328, 639
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 119
BbvI GCAGC 7 cut(s) 134, 214, 275, 409, 459, 462, 556
BccI CCATC 1 cut(s) 743
BceAI ACGGC 3 cut(s) 153, 334, 544
BciT130I CCWGG 2 cut(s) 142, 289
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 278, 651
Bme1390I CCNGG 3 cut(s) 142, 182, 289
Bme18I GGWCC 2 cut(s) 145, 553
BmeRI GACNNNNNGTC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 3 cut(s) 54, 145, 553
BmiI GGNNCC 4 cut(s) 146, 235, 555, 593
BmrFI CCNGG 3 cut(s) 142, 182, 289
BmsI GCATC 1 cut(s) 404
BpiI GAAGAC 1 cut(s) 492
Bpu10I CCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 182
BsaAI YACGTR 3 cut(s) 328, 639, 711
BsaHI GRCGYC 3 cut(s) 77, 332, 498
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 6 cut(s) 135, 141, 180, 288, 316, 373
BsaWI WCCGGW 1 cut(s) 680
BsaXI ACNNNNNCTCC 4 cut(s) 445, 475, 623, 653
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 161, 294
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 105, 604
Bse3DI GCAATG 1 cut(s) 572
BseBI CCWGG 2 cut(s) 142, 289
BseDI CCNNGG 6 cut(s) 135, 141, 180, 288, 316, 373
BseGI GGATG 1 cut(s) 754
BseLI CCNNNNNNNGG 3 cut(s) 141, 161, 294
BseMI GCAATG 1 cut(s) 572
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 2 cut(s) 105, 604
BsePI GCGCGC 2 cut(s) 256, 440
BseRI GAGGAG 1 cut(s) 310
BseX3I CGGCCG 1 cut(s) 309
BseXI GCAGC 7 cut(s) 134, 214, 275, 409, 459, 462, 556
Bsh1236I CGCG 6 cut(s) 159, 256, 258, 260, 442, 444
Bsh1285I CGRYCG 2 cut(s) 312, 589
BshFI GGCC 7 cut(s) 56, 111, 140, 311, 315, 628, 660
BsiEI CGRYCG 2 cut(s) 312, 589
BsiHKAI GWGCWC 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 8 cut(s) 112, 182, 199, 308, 312, 629, 669, 681
BslFI GGGAC 4 cut(s) 18, 158, 163, 341
BslI CCNNNNNNNGG 3 cut(s) 141, 161, 294
BsmAI GTCTC 2 cut(s) 278, 651
BsmFI GGGAC 4 cut(s) 18, 158, 163, 341
BsnI GGCC 7 cut(s) 56, 111, 140, 311, 315, 628, 660
Bso31I GGTCTC 1 cut(s) 278
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 1 cut(s) 119
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 3 cut(s) 37, 217, 591
BspACI CCGC 5 cut(s) 260, 278, 444, 538, 672
BspANI GGCC 7 cut(s) 56, 111, 140, 311, 315, 628, 660
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 6 cut(s) 159, 256, 258, 260, 442, 444
BspLI GGNNCC 4 cut(s) 146, 235, 555, 593
BspPI GGATC 3 cut(s) 45, 586, 599
BspQI GCTCTTC 1 cut(s) 610
BspTNI GGTCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 572
BsrFI RCCGGY 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 2 cut(s) 105, 604
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 6 cut(s) 135, 141, 180, 288, 316, 373
BssHII GCGCGC 2 cut(s) 256, 440
BssMI GATC 3 cut(s) 37, 217, 591
BssNAI GTATAC 1 cut(s) 21
BssNI GRCGYC 3 cut(s) 77, 332, 498
Bst1107I GTATAC 1 cut(s) 21
Bst2UI CCWGG 2 cut(s) 142, 289
Bst4CI ACNGT 4 cut(s) 175, 304, 491, 587
Bst6I CTCTTC 1 cut(s) 610
BstACI GRCGYC 3 cut(s) 77, 332, 498
BstAPI GCANNNNNTGC 1 cut(s) 422
BstAUI TGTACA 1 cut(s) 429
BstBAI YACGTR 3 cut(s) 328, 639, 711
BstC8I GCNNGC 7 cut(s) 58, 238, 258, 313, 442, 473, 658
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 2 cut(s) 135, 316
BstF5I GGATG 1 cut(s) 754
BstFNI CGCG 6 cut(s) 159, 256, 258, 260, 442, 444
BstHHI GCGC 4 cut(s) 258, 260, 442, 444
BstKTI GATC 3 cut(s) 40, 220, 594
BstMAI GTCTC 2 cut(s) 278, 651
BstMBI GATC 3 cut(s) 37, 217, 591
BstMCI CGRYCG 2 cut(s) 312, 589
BstMWI GCNNNNNNNGC 3 cut(s) 413, 422, 537
BstNI CCWGG 2 cut(s) 142, 289
BstNSI RCATGY 2 cut(s) 356, 646
BstSCI CCNGG 3 cut(s) 140, 180, 287
BstUI CGCG 6 cut(s) 159, 256, 258, 260, 442, 444
BstV1I GCAGC 7 cut(s) 134, 214, 275, 409, 459, 462, 556
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 591
BstYI RGATCY 1 cut(s) 591
BstZ17I GTATAC 1 cut(s) 21
BstZI CGGCCG 1 cut(s) 309
BsuRI GGCC 7 cut(s) 56, 111, 140, 311, 315, 628, 660
BtgI CCRYGG 2 cut(s) 135, 316
BtgZI GCGATG 1 cut(s) 325
BtsCI GGATG 1 cut(s) 754
BtsIMutI CAGTG 1 cut(s) 98
Cac8I GCNNGC 7 cut(s) 58, 238, 258, 313, 442, 473, 658
CfoI GCGC 4 cut(s) 258, 260, 442, 444
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 54, 145, 553
CseI GACGC 1 cut(s) 321
Csp6I GTAC 3 cut(s) 64, 430, 712
CviAII CATG 3 cut(s) 353, 427, 643
CviQI GTAC 3 cut(s) 64, 430, 712
DdeI CTNAG 1 cut(s) 384
DpnI GATC 3 cut(s) 39, 219, 593
DpnII GATC 3 cut(s) 37, 217, 591
DrdI GACNNNNNNGTC 1 cut(s) 82
DriI GACNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 4 cut(s) 109, 309, 313, 626
EagI CGGCCG 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 610
Eam1105I GACNNNNNGTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 610
EciI GGCGGA 1 cut(s) 527
EclXI CGGCCG 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 278
Eco47I GGWCC 2 cut(s) 145, 553
Eco52I CGGCCG 1 cut(s) 309
Eco72I CACGTG 2 cut(s) 328, 639
Eco88I CYCGRG 1 cut(s) 578
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 2 cut(s) 140, 287
FaeI CATG 3 cut(s) 356, 430, 646
FaqI GGGAC 4 cut(s) 18, 158, 163, 341
FatI CATG 3 cut(s) 352, 426, 642
FblI GTMKAC 2 cut(s) 20, 95
FseI GGCCGGCC 1 cut(s) 315
GlaI GCGC 4 cut(s) 257, 259, 441, 443
HaeIII GGCC 7 cut(s) 56, 111, 140, 311, 315, 628, 660
HapII CCGG 8 cut(s) 112, 182, 199, 308, 312, 629, 669, 681
HgaI GACGC 1 cut(s) 321
HhaI GCGC 4 cut(s) 258, 260, 442, 444
Hin1I GRCGYC 3 cut(s) 77, 332, 498
Hin1II CATG 3 cut(s) 356, 430, 646
Hin6I GCGC 4 cut(s) 256, 258, 440, 442
HinP1I GCGC 4 cut(s) 256, 258, 440, 442
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HinfI GANTC 4 cut(s) 187, 195, 251, 460
HpaII CCGG 8 cut(s) 112, 182, 199, 308, 312, 629, 669, 681
HphI GGTGA 1 cut(s) 670
Hpy166II GTNNAC 2 cut(s) 21, 96
Hpy188I TCNGA 2 cut(s) 192, 591
Hpy188III TCNNGA 4 cut(s) 26, 399, 580, 746
Hpy8I GTNNAC 2 cut(s) 21, 96
Hpy99I CGWCG 4 cut(s) 79, 500, 530, 722
HpyCH4III ACNGT 4 cut(s) 175, 304, 491, 587
HpyCH4IV ACGT 5 cut(s) 77, 327, 498, 638, 710
HpyCH4V TGCA 6 cut(s) 212, 395, 572, 642, 656, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 422, 537
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 5 cut(s) 77, 327, 498, 638, 710
Hsp92I GRCGYC 3 cut(s) 77, 332, 498
Hsp92II CATG 3 cut(s) 356, 430, 646
HspAI GCGC 4 cut(s) 256, 258, 440, 442
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
Kzo9I GATC 3 cut(s) 37, 217, 591
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 3 cut(s) 114, 233, 412
Lsp1109I GCAGC 7 cut(s) 134, 214, 275, 409, 459, 462, 556
LweI GCATC 1 cut(s) 404
MaeII ACGT 5 cut(s) 77, 327, 498, 638, 710
MaeIII GTNAC 1 cut(s) 169
MalI GATC 3 cut(s) 39, 219, 593
MauBI CGCGCGCG 1 cut(s) 256
MboI GATC 3 cut(s) 37, 217, 591
MboII GAAGA 3 cut(s) 497, 597, 746
MfeI CAATTG 1 cut(s) 727
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 3 cut(s) 267, 435, 727
MlyI GAGTC 2 cut(s) 245, 469
MmeI TCCRAC 2 cut(s) 142, 569
MnlI CCTC 6 cut(s) 165, 288, 291, 376, 383, 545
MroNI GCCGGC 1 cut(s) 311
MslI CAYNNNNRTG 3 cut(s) 326, 641, 747
MspI CCGG 8 cut(s) 112, 182, 199, 308, 312, 629, 669, 681
MspR9I CCNGG 3 cut(s) 142, 182, 289
MunI CAATTG 1 cut(s) 727
MvaI CCWGG 2 cut(s) 142, 289
MvnI CGCG 6 cut(s) 159, 256, 258, 260, 442, 444
MwoI GCNNNNNNNGC 3 cut(s) 413, 422, 537
NaeI GCCGGC 1 cut(s) 313
NciI CCSGG 1 cut(s) 182
NdeII GATC 3 cut(s) 37, 217, 591
NgoMIV GCCGGC 1 cut(s) 311
NlaIII CATG 3 cut(s) 356, 430, 646
NlaIV GGNNCC 4 cut(s) 146, 235, 555, 593
NmeAIII GCCGAG 1 cut(s) 354
NspI RCATGY 2 cut(s) 356, 646
OliI CACNNNNGTG 1 cut(s) 326
PauI GCGCGC 2 cut(s) 256, 440
PceI AGGCCT 1 cut(s) 660
PciI ACATGT 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 610
PcsI WCGNNNNNNNCGW 3 cut(s) 92, 324, 495
PdiI GCCGGC 1 cut(s) 313
PfeI GAWTC 2 cut(s) 187, 195
PflMI CCANNNNNTGG 1 cut(s) 141
PleI GAGTC 2 cut(s) 245, 468
PmaCI CACGTG 2 cut(s) 328, 639
PmlI CACGTG 2 cut(s) 328, 639
PpsI GAGTC 2 cut(s) 245, 468
Ppu21I YACGTR 3 cut(s) 328, 639, 711
PpuMI RGGWCCY 1 cut(s) 553
PscI ACATGT 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 2 cut(s) 140, 287
PspCI CACGTG 2 cut(s) 328, 639
PspGI CCWGG 2 cut(s) 140, 287
PspN4I GGNNCC 4 cut(s) 146, 235, 555, 593
PspPI GGNCC 3 cut(s) 54, 145, 553
PspPPI RGGWCCY 1 cut(s) 553
PsuI RGATCY 1 cut(s) 591
PteI GCGCGC 2 cut(s) 256, 440
RigI GGCCGGCC 1 cut(s) 315
RsaI GTAC 3 cut(s) 65, 431, 713
RsaNI GTAC 3 cut(s) 64, 430, 712
RseI CAYNNNNRTG 3 cut(s) 326, 641, 747
SalI GTCGAC 1 cut(s) 94
SapI GCTCTTC 1 cut(s) 610
Sau3AI GATC 3 cut(s) 37, 217, 591
Sau96I GGNCC 3 cut(s) 54, 145, 553
SchI GAGTC 2 cut(s) 245, 469
ScrFI CCNGG 3 cut(s) 142, 182, 289
SduI GDGCHC 1 cut(s) 119
SfaNI GCATC 1 cut(s) 404
SinI GGWCC 2 cut(s) 145, 553
SmiMI CAYNNNNRTG 3 cut(s) 326, 641, 747
SmlI CTYRAG 1 cut(s) 24
SmoI CTYRAG 1 cut(s) 24
Sse9I AATT 3 cut(s) 267, 435, 727
SseBI AGGCCT 1 cut(s) 660
SsiI CCGC 5 cut(s) 260, 278, 444, 538, 672
StuI AGGCCT 1 cut(s) 660
StyD4I CCNGG 3 cut(s) 140, 180, 287
TaaI ACNGT 4 cut(s) 175, 304, 491, 587
TaiI ACGT 5 cut(s) 80, 330, 501, 641, 713
TaqI TCGA 5 cut(s) 40, 74, 95, 220, 717
TasI AATT 3 cut(s) 267, 435, 727
TatI WGTACW 1 cut(s) 429
TauI GCSGC 3 cut(s) 263, 447, 675
TfiI GAWTC 2 cut(s) 187, 195
TscAI CASTG 1 cut(s) 105
TseI GCWGC 7 cut(s) 122, 202, 263, 422, 447, 475, 569
TspGWI ACGGA 1 cut(s) 687
TspRI CASTG 1 cut(s) 105
Van91I CCANNNNNTGG 1 cut(s) 141
VpaK11BI GGWCC 2 cut(s) 145, 553
XceI RCATGY 2 cut(s) 356, 646
XmiI GTMKAC 2 cut(s) 20, 95
ZraI GACGTC 2 cut(s) 78, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.