Rh6CG477100

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0022551
Physical Location & Seq
Forward (+)
64554689 .. 64555150
462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG477100.1

Sequence Viewer

Length: 462 bp
ATGAAGAAACTGAGAAAGGAAAAAAAGAGAGAGAGAGAACCGGCAAACCAGGCCTTTTGCAACAGAAAGCTCATAGGAGCCAGACACTTCACTCGCGGCAGCAATTTTACTTCCGCAAGAGACCAGGGCGAGGAGGACAGTTCCGGCCACGGCACACACGTGGCGTCAATCATCGCTGGACGACATGTCCCAAACGCCTCCTTCCCCGGCTTTGCTCAAGGTGTTGCATCAGGAGTTGCTCCAAACGCAAGACTTGCCATGTACAGAATTGCGCGCGGGAGCAGGAGACTGAGTCTAACTTGCTGGCTGCCCGTAGAAGACGGTTGCGACGTCATATCTTTGTCATTGTCATTAGACGACGGCGACCTCCGCCCTTATCACAAGGACCCGATTGCTATAGCTGCATTGTCCGCAACCCATATGCCTTGTTTCGGCCTCCGCAAGCAACGGCGACCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

153

Amino Acids

16.81

Weight (kDa)

10.18

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 31 - 132 5.2e-11 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022551)

Species Orthologous Gene IDs
pyrus_communis pycom11g07670
rosa_chinensis RchiOBHm_Chr6g0306571
rosa_roxburghii Rroxscaffold_7G00161660
rosa_samantha Rh6CG477100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 333
AccII CGCG 3 cut(s) 96, 274, 276
AciI CCGC 6 cut(s) 96, 114, 276, 370, 411, 439
AcoI YGGCCR 1 cut(s) 145
AcvI CACGTG 2 cut(s) 160, 459
AcyI GRCGYC 2 cut(s) 164, 330
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 2 cut(s) 130, 431
AflIII ACRYGT 2 cut(s) 157, 184
AhdI GACNNNNNGTC 1 cut(s) 185
AjnI CCWGG 2 cut(s) 48, 123
AleI CACNNNNGTG 1 cut(s) 158
AluBI AGCT 2 cut(s) 70, 401
AluI AGCT 2 cut(s) 70, 401
Alw26I GTCTC 2 cut(s) 114, 280
AoxI GGCC 3 cut(s) 51, 145, 433
ApeKI GCWGC 3 cut(s) 99, 307, 401
AspLEI GCGC 2 cut(s) 274, 276
AspS9I GGNCC 1 cut(s) 385
AsuC2I CCSGG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 385
BbrPI CACGTG 2 cut(s) 160, 459
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 3 cut(s) 111, 294, 388
BceAI ACGGC 2 cut(s) 166, 376
BciT130I CCWGG 2 cut(s) 50, 125
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 114, 280
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 4 cut(s) 97, 100, 308, 402
BlsI GCNGC 4 cut(s) 98, 101, 309, 403
Bme1390I CCNGG 3 cut(s) 50, 125, 207
Bme18I GGWCC 1 cut(s) 385
BmeRI GACNNNNNGTC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 2 cut(s) 79, 387
BmrFI CCNGG 3 cut(s) 50, 125, 207
BmsI GCATC 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 324
BpuEI CTTGAG 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 207
BsaAI YACGTR 2 cut(s) 160, 459
BsaHI GRCGYC 2 cut(s) 164, 330
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 124, 148, 205
BsaXI ACNNNNNCTCC 2 cut(s) 277, 307
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 431
Bse118I RCCGGY 1 cut(s) 40
BseBI CCWGG 2 cut(s) 50, 125
BseDI CCNNGG 3 cut(s) 124, 148, 205
BseLI CCNNNNNNNGG 2 cut(s) 130, 431
BseMII CTCAG 1 cut(s) 281
BsePI GCGCGC 1 cut(s) 272
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 3 cut(s) 111, 294, 388
Bsh1236I CGCG 3 cut(s) 96, 274, 276
BshFI GGCC 3 cut(s) 53, 147, 435
BsiSI CCGG 3 cut(s) 41, 144, 207
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 130, 431
BsmAI GTCTC 2 cut(s) 114, 280
BsmFI GGGAC 1 cut(s) 173
BsnI GGCC 3 cut(s) 53, 147, 435
Bso31I GGTCTC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 261
BspACI CCGC 6 cut(s) 96, 114, 276, 370, 411, 439
BspANI GGCC 3 cut(s) 53, 147, 435
BspCNI CTCAG 1 cut(s) 282
BspFNI CGCG 3 cut(s) 96, 274, 276
BspLI GGNNCC 2 cut(s) 79, 387
BspTNI GGTCTC 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 40
BsrGI TGTACA 1 cut(s) 261
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 3 cut(s) 124, 148, 205
BssHII GCGCGC 1 cut(s) 272
BssNI GRCGYC 2 cut(s) 164, 330
Bst2UI CCWGG 2 cut(s) 50, 125
Bst4CI ACNGT 2 cut(s) 140, 323
BstACI GRCGYC 2 cut(s) 164, 330
BstAPI GCANNNNNTGC 1 cut(s) 254
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 2 cut(s) 160, 459
BstC8I GCNNGC 3 cut(s) 274, 305, 443
BstDEI CTNAG 2 cut(s) 11, 290
BstDSI CCRYGG 1 cut(s) 148
BstFNI CGCG 3 cut(s) 96, 274, 276
BstHHI GCGC 2 cut(s) 274, 276
BstMAI GTCTC 2 cut(s) 114, 280
BstMWI GCNNNNNNNGC 6 cut(s) 50, 245, 254, 369, 401, 410
BstNI CCWGG 2 cut(s) 50, 125
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 3 cut(s) 48, 123, 205
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 3 cut(s) 96, 274, 276
BstV1I GCAGC 3 cut(s) 111, 294, 388
BstV2I GAAGAC 1 cut(s) 324
BsuRI GGCC 3 cut(s) 53, 147, 435
BtgI CCRYGG 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 157
Cac8I GCNNGC 3 cut(s) 274, 305, 443
CfoI GCGC 2 cut(s) 274, 276
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 385
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 262
CviAII CATG 2 cut(s) 185, 259
CviJI RGCY 8 cut(s) 53, 70, 80, 147, 210, 307, 401, 435
CviKI_1 RGCY 8 cut(s) 53, 70, 80, 147, 210, 307, 401, 435
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 2 cut(s) 11, 290
DriI GACNNNNNGTC 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 145
Eam1105I GACNNNNNGTC 1 cut(s) 185
EciI GGCGGA 1 cut(s) 359
Eco147I AGGCCT 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 385
Eco72I CACGTG 2 cut(s) 160, 459
EcoO109I RGGNCCY 1 cut(s) 385
EcoRII CCWGG 2 cut(s) 48, 123
FaeI CATG 2 cut(s) 188, 262
FaiI YATR 7 cut(s) 74, 186, 260, 335, 398, 420, 422
FaqI GGGAC 1 cut(s) 173
FatI CATG 2 cut(s) 184, 258
FauI CCCGC 1 cut(s) 269
FauNDI CATATG 1 cut(s) 420
Fnu4HI GCNGC 4 cut(s) 97, 100, 308, 402
Fsp4HI GCNGC 4 cut(s) 97, 100, 308, 402
GlaI GCGC 2 cut(s) 273, 275
GluI GCNGC 4 cut(s) 97, 100, 308, 402
HaeIII GGCC 3 cut(s) 53, 147, 435
HapII CCGG 3 cut(s) 41, 144, 207
HgaI GACGC 1 cut(s) 153
HhaI GCGC 2 cut(s) 274, 276
Hin1I GRCGYC 2 cut(s) 164, 330
Hin1II CATG 2 cut(s) 188, 262
Hin6I GCGC 2 cut(s) 272, 274
HinP1I GCGC 2 cut(s) 272, 274
HinfI GANTC 1 cut(s) 292
HpaII CCGG 3 cut(s) 41, 144, 207
Hpy188III TCNNGA 1 cut(s) 231
Hpy99I CGWCG 2 cut(s) 332, 362
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 140, 323
HpyCH4IV ACGT 3 cut(s) 159, 330, 458
HpyCH4V TGCA 3 cut(s) 60, 227, 404
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 245, 254, 369, 401, 410
HpyF3I CTNAG 2 cut(s) 11, 290
HpySE526I ACGT 3 cut(s) 159, 330, 458
Hsp92I GRCGYC 2 cut(s) 164, 330
Hsp92II CATG 2 cut(s) 188, 262
HspAI GCGC 2 cut(s) 272, 274
LmnI GCTCC 3 cut(s) 77, 244, 279
Lsp1109I GCAGC 3 cut(s) 111, 294, 388
LweI GCATC 1 cut(s) 236
MaeII ACGT 3 cut(s) 159, 330, 458
MboII GAAGA 2 cut(s) 16, 329
MluCI AATT 2 cut(s) 103, 267
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 5 cut(s) 124, 127, 208, 377, 446
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 3 cut(s) 41, 144, 207
MspR9I CCNGG 3 cut(s) 50, 125, 207
MvaI CCWGG 2 cut(s) 50, 125
MvnI CGCG 3 cut(s) 96, 274, 276
MwoI GCNNNNNNNGC 6 cut(s) 50, 245, 254, 369, 401, 410
NciI CCSGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 420
NlaIII CATG 2 cut(s) 188, 262
NlaIV GGNNCC 2 cut(s) 79, 387
NspI RCATGY 1 cut(s) 188
OliI CACNNNNGTG 1 cut(s) 158
PauI GCGCGC 1 cut(s) 272
PceI AGGCCT 1 cut(s) 53
PciI ACATGT 1 cut(s) 184
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 327
PflFI GACNNNGTC 1 cut(s) 291
PkrI GCNGC 4 cut(s) 98, 101, 309, 403
PleI GAGTC 1 cut(s) 300
PmaCI CACGTG 2 cut(s) 160, 459
PmlI CACGTG 2 cut(s) 160, 459
PpsI GAGTC 1 cut(s) 300
Ppu21I YACGTR 2 cut(s) 160, 459
PpuMI RGGWCCY 1 cut(s) 385
PscI ACATGT 1 cut(s) 184
Psp5II RGGWCCY 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 48, 123
PspCI CACGTG 2 cut(s) 160, 459
PspGI CCWGG 2 cut(s) 48, 123
PspN4I GGNNCC 2 cut(s) 79, 387
PspPI GGNCC 1 cut(s) 385
PspPPI RGGWCCY 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 291
PteI GCGCGC 1 cut(s) 272
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 158
SatI GCNGC 4 cut(s) 97, 100, 308, 402
Sau96I GGNCC 1 cut(s) 385
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 3 cut(s) 50, 125, 207
SetI ASST 7 cut(s) 72, 162, 223, 333, 369, 403, 461
SfaNI GCATC 1 cut(s) 236
SfcI CTRYAG 1 cut(s) 396
SinI GGWCC 1 cut(s) 385
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
Sse9I AATT 2 cut(s) 103, 267
SseBI AGGCCT 1 cut(s) 53
SsiI CCGC 6 cut(s) 96, 114, 276, 370, 411, 439
StuI AGGCCT 1 cut(s) 53
StyD4I CCNGG 3 cut(s) 48, 123, 205
TaaI ACNGT 2 cut(s) 140, 323
TaiI ACGT 3 cut(s) 162, 333, 461
TasI AATT 2 cut(s) 103, 267
TatI WGTACW 1 cut(s) 261
TauI GCSGC 1 cut(s) 99
TseI GCWGC 3 cut(s) 99, 307, 401
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 385
XceI RCATGY 1 cut(s) 188
ZraI GACGTC 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.