pycom12397g00100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0019809
Physical Location & Seq
Forward (+)
47656 .. 49267
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTCACTGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAAGGCGAAGAAGGCCCCCTTCCGGGGGGGCCCGAGCCTCCCAAAAGGTAACGGAGGCGTGCAAAGGTTTCCTCGGGCCAGACGGAGATTGGCCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTGCAAGACCCACCCGTCGAGCAGGGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACTCTAGGGATAACAGGTCGTCTTATTCAAAACCCCAATCCAATTATGAAATCCCTTCTCTCCCACTTCACACCTCGGAACGCACCGTTCTTATATAGAGAGAGGCGCTTTCACATCTTCTTAACCGGAAACGGCTGGGGAGAGGAAAGGTTCCTTTTTTTTAGGGTACTCCCGGGAACAGATCCAGTGGAGACGGGGTGGGGCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

19.26

Weight (kDa)

11.32

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019809)

Species Orthologous Gene IDs
pyrus_communis pycom01g11770 pycom12397g00100
rosa_multiflora Rmu_sc0002678.1_g000001 Rmu_sc0003576.1_g000002 Rmu_sc0009780.1_g000004
rosa_rugosa Rorug01G0444200 Rorug04G0209300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 240
Acc16I TGCGCA 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 32
AccB1I GGYRCC 1 cut(s) 280
AciI CCGC 1 cut(s) 16
AclWI GGATC 2 cut(s) 266, 494
AfaI GTAC 1 cut(s) 486
AfiI CCNNNNNNNGG 6 cut(s) 30, 71, 125, 126, 127, 128
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 2 cut(s) 29, 64
AluBI AGCT 3 cut(s) 53, 96, 222
AluI AGCT 3 cut(s) 53, 96, 222
Alw26I GTCTC 1 cut(s) 503
AlwI GGATC 2 cut(s) 266, 494
Ama87I CYCGRG 4 cut(s) 135, 176, 199, 490
AoxI GGCC 8 cut(s) 80, 116, 132, 179, 194, 262, 294, 520
ApaI GGGCCC 1 cut(s) 136
ApeKI GCWGC 1 cut(s) 93
AspLEI GCGC 2 cut(s) 93, 426
AspS9I GGNCC 8 cut(s) 80, 117, 132, 133, 179, 195, 294, 520
AsuC2I CCSGG 3 cut(s) 127, 491, 492
AvaI CYCGRG 4 cut(s) 135, 176, 199, 490
BaeGI GKGCMC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 105
BceAI ACGGC 2 cut(s) 281, 466
BciT130I CCWGG 2 cut(s) 31, 66
BcnI CCSGG 3 cut(s) 127, 491, 492
BcoDI GTCTC 1 cut(s) 503
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 524
BfoI RGCGCY 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 32
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
Bme1390I CCNGG 5 cut(s) 31, 66, 127, 491, 492
BmeT110I CYCGRG 4 cut(s) 135, 176, 199, 490
BmgT120I GGNCC 8 cut(s) 80, 117, 132, 133, 179, 195, 294, 520
BmiI GGNNCC 6 cut(s) 119, 133, 134, 282, 470, 521
BmrFI CCNGG 5 cut(s) 31, 66, 127, 491, 492
BmsI GCATC 1 cut(s) 3
BoxI GACNNNNGTC 1 cut(s) 257
Bpu10I CCTNAGC 1 cut(s) 97
BpuMI CCSGG 3 cut(s) 127, 491, 492
BsaJI CCNNGG 6 cut(s) 64, 65, 126, 175, 392, 490
BsaWI WCCGGW 1 cut(s) 443
BsaXI ACNNNNNCTCC 1 cut(s) 500
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 71, 125, 126, 127, 128
Bse1I ACTGG 1 cut(s) 503
BseBI CCWGG 2 cut(s) 31, 66
BseDI CCNNGG 6 cut(s) 64, 65, 126, 175, 392, 490
BseLI CCNNNNNNNGG 6 cut(s) 30, 71, 125, 126, 127, 128
BseNI ACTGG 1 cut(s) 503
BseSI GKGCMC 1 cut(s) 136
BseXI GCAGC 1 cut(s) 105
BseYI CCCAGC 1 cut(s) 453
BsgI GTGCAG 1 cut(s) 10
BshFI GGCC 8 cut(s) 82, 118, 134, 181, 196, 264, 296, 522
BshNI GGYRCC 1 cut(s) 280
BsiHKCI CYCGRG 4 cut(s) 135, 176, 199, 490
BsiSI CCGG 3 cut(s) 126, 444, 491
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 6 cut(s) 30, 71, 125, 126, 127, 128
BsmAI GTCTC 1 cut(s) 503
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 8 cut(s) 82, 118, 134, 181, 196, 264, 296, 522
BsoBI CYCGRG 4 cut(s) 135, 176, 199, 490
Bsp120I GGGCCC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 271, 499
BspACI CCGC 1 cut(s) 16
BspANI GGCC 8 cut(s) 82, 118, 134, 181, 196, 264, 296, 522
BspLI GGNNCC 6 cut(s) 119, 133, 134, 282, 470, 521
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 2 cut(s) 266, 494
BspT107I GGYRCC 1 cut(s) 280
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 6 cut(s) 64, 65, 126, 175, 392, 490
BssMI GATC 2 cut(s) 271, 499
Bst2UI CCWGG 2 cut(s) 31, 66
Bst4CI ACNGT 3 cut(s) 60, 280, 405
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 2 cut(s) 97, 266
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 93, 426
BstKTI GATC 2 cut(s) 274, 502
BstMAI GTCTC 1 cut(s) 503
BstMBI GATC 2 cut(s) 271, 499
BstMWI GCNNNNNNNGC 2 cut(s) 88, 115
BstNI CCWGG 2 cut(s) 31, 66
BstPAI GACNNNNGTC 1 cut(s) 257
BstSCI CCNGG 5 cut(s) 29, 64, 125, 489, 490
BstSFI CTRYAG 1 cut(s) 524
BstSLI GKGCMC 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 8 cut(s) 82, 118, 134, 181, 196, 264, 296, 522
BtsIMutI CAGTG 2 cut(s) 56, 510
BveI ACCTGC 1 cut(s) 32
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 2 cut(s) 93, 426
Cfr13I GGNCC 8 cut(s) 80, 117, 132, 133, 179, 195, 294, 520
Cfr9I CCCGGG 1 cut(s) 490
Csp6I GTAC 1 cut(s) 485
CviQI GTAC 1 cut(s) 485
DdeI CTNAG 2 cut(s) 97, 266
DpnI GATC 2 cut(s) 273, 501
DpnII GATC 2 cut(s) 271, 499
DrdI GACNNNNNNGTC 1 cut(s) 240
DseDI GACNNNNNNGTC 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 136
Eco88I CYCGRG 4 cut(s) 135, 176, 199, 490
EcoO109I RGGNCCY 3 cut(s) 117, 132, 520
EcoRII CCWGG 2 cut(s) 29, 64
EcoT38I GRGCYC 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 503
FaiI YATR 4 cut(s) 48, 365, 412, 414
FalI AAGNNNNNCTT 4 cut(s) 106, 138, 249, 281
FaqI GGGAC 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 94
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 1 cut(s) 94
FspBI CTAG 1 cut(s) 323
FspI TGCGCA 1 cut(s) 92
GlaI GCGC 2 cut(s) 92, 425
GluI GCNGC 1 cut(s) 94
GsaI CCCAGC 1 cut(s) 457
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 8 cut(s) 82, 118, 134, 181, 196, 264, 296, 522
HapII CCGG 3 cut(s) 126, 444, 491
HhaI GCGC 2 cut(s) 93, 426
Hin6I GCGC 2 cut(s) 91, 424
HinP1I GCGC 2 cut(s) 91, 424
HindIII AAGCTT 1 cut(s) 51
HpaII CCGG 3 cut(s) 126, 444, 491
Hpy188I TCNGA 2 cut(s) 276, 396
Hpy99I CGWCG 3 cut(s) 246, 280, 302
HpyAV CCTTC 6 cut(s) 99, 108, 132, 209, 285, 383
HpyCH4III ACNGT 3 cut(s) 60, 280, 405
HpyCH4V TGCA 4 cut(s) 27, 165, 206, 230
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 115
HpyF3I CTNAG 2 cut(s) 97, 266
HspAI GCGC 2 cut(s) 91, 424
Kzo9I GATC 2 cut(s) 271, 499
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 1 cut(s) 105
LweI GCATC 1 cut(s) 3
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 151, 316
MalI GATC 2 cut(s) 273, 501
MboI GATC 2 cut(s) 271, 499
MboII GAAGA 3 cut(s) 22, 123, 427
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 136
MluCI AATT 1 cut(s) 360
MmeI TCCRAC 1 cut(s) 299
MnlI CCTC 7 cut(s) 151, 151, 185, 208, 402, 414, 454
MseI TTAA 1 cut(s) 440
MspI CCGG 3 cut(s) 126, 444, 491
MspR9I CCNGG 5 cut(s) 31, 66, 127, 491, 492
MvaI CCWGG 2 cut(s) 31, 66
MwoI GCNNNNNNNGC 2 cut(s) 88, 115
NciI CCSGG 3 cut(s) 127, 491, 492
NdeII GATC 2 cut(s) 271, 499
NlaIV GGNNCC 6 cut(s) 119, 133, 134, 282, 470, 521
NmeAIII GCCGAG 1 cut(s) 309
NsbI TGCGCA 1 cut(s) 92
PaeR7I CTCGAG 1 cut(s) 199
PasI CCCWGGG 1 cut(s) 65
PkrI GCNGC 1 cut(s) 95
PshAI GACNNNNGTC 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 29, 64
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 2 cut(s) 29, 64
PspN4I GGNNCC 6 cut(s) 119, 133, 134, 282, 470, 521
PspOMI GGGCCC 1 cut(s) 132
PspPI GGNCC 8 cut(s) 80, 117, 132, 133, 179, 195, 294, 520
PspXI VCTCGAGB 1 cut(s) 199
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 1 cut(s) 486
RsaNI GTAC 1 cut(s) 485
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 2 cut(s) 271, 499
Sau96I GGNCC 8 cut(s) 80, 117, 132, 133, 179, 195, 294, 520
ScrFI CCNGG 5 cut(s) 31, 66, 127, 491, 492
SduI GDGCHC 1 cut(s) 136
SfaNI GCATC 1 cut(s) 3
SfcI CTRYAG 1 cut(s) 524
Sfr274I CTCGAG 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 199
SmaI CCCGGG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 1 cut(s) 360
SsiI CCGC 1 cut(s) 16
SspMI CTAG 1 cut(s) 323
StyD4I CCNGG 5 cut(s) 29, 64, 125, 489, 490
TaaI ACNGT 3 cut(s) 60, 280, 405
TaqI TCGA 2 cut(s) 200, 244
TasI AATT 1 cut(s) 360
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 2 cut(s) 63, 510
TseI GCWGC 1 cut(s) 93
TspDTI ATGAA 2 cut(s) 63, 380
TspGWI ACGGA 3 cut(s) 170, 201, 322
TspMI CCCGGG 1 cut(s) 490
TspRI CASTG 2 cut(s) 63, 510
XcmI CCANNNNNNNNNTGG 1 cut(s) 189
XhoI CTCGAG 1 cut(s) 199
XmaI CCCGGG 1 cut(s) 490
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.