pycom12397g00220

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0020230
Physical Location & Seq
Reverse (-)
118760 .. 119722
963 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12397g00220.1

Sequence Viewer

Length: 963 bp
ATGCAAACTAAAAATACCTTTTCTTGGATAAAGAAAGAGATTACTCGATCCATTTCCGTATCGCTCATGATATATATACTAACCCAGGCACCCCTTTCAAATGCATATCCCATTTTTGCACAGCAGGGTTATGAAAATCCACGAGAAGCGACTGGCCGTATTGTATGTGCCAATTGCCATTTAGCTAATAAACCCGTGGATATCGAGGTTCCACAAGCGGTACTTCCTGATACTGTATTTGAAGCAGTTGTTCGAATTCCTTATGATCTGCAACTGAAACAAGTTCTTGCTAATGGTAAAAAAGGAGCTTTGAATGTCGGGGCTGTTCTGATTTTACCCGAGGGGTTTGAATTAGCCCCTCCCGATCGTATTTCGCCCGAGATTAAAGAAAAGATAGGAAATCTGTCTTTTCAGAGCTATCGTCCCACTAAAAAAAATATTCTTGTGATAGGTCCGGTTCCTGGTCAGAAATATAGTGAAATCACCTTTCCTATTCTTTCCCCGGACCCCGCTACTAAGAAAGATGTGCACTTCTTAAAATATCCCATATATGTAGGCGGGAACAGGGGAAGGGGTCAGATTTATCCCGACGGGAGCAAGAGTAACAATAATGTTTATAATGCTACAGCAGCAGGTATAGTAAGCAAAATAATACGAAAAGAAAAAGGGGGGTACGAAATAACCATAGCGGATGCATCGGATGGACGTCAAGTGGTTGATATTATCCCTCCAGGACCGGAACTTCTTGTTTCAGAGGGCGAATCCATCAAACTTGATCAACCATTAACGAGTAATCCTAATGTGGGTGGATTTGGTCAGGGAGATGCGGAAATAGTACTTCAAGATCCATTACGTGTCCAAGGTCTTTTGCTCTTCTTGGCATCTGTTATTTTGGCACAAATCTTTTTGGTTCTTAAAAAGAAACAGTTTGAGAAGGTTCAATTGTCCGAAATGAATTTCTAG

Protein Analysis

321

Amino Acids

35.08

Weight (kDa)

8.83

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Apocytochr_F_N PF16639 36 - 190 3.6e-79 Apocytochrome F, N-terminal
Apocytochr_F_C PF01333 202 - 320 4.7e-51 Apocytochrome F, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020230)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00540
malus_domestica MD09G1098000.v1.1 MD12G1014500.v1.1
pyrus_communis pycom12397g00220
rosa_chinensis RchiOBHm_CPg0502071
rosa_rugosa Rorug02G0490700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AatII GACGTC 1 cut(s) 709
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 88
AciI CCGC 5 cut(s) 218, 510, 558, 689, 827
AclWI GGATC 2 cut(s) 42, 839
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 2 cut(s) 255, 955
AcyI GRCGYC 1 cut(s) 706
AfaI GTAC 3 cut(s) 222, 674, 837
AfiI CCNNNNNNNGG 3 cut(s) 24, 461, 803
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 6 cut(s) 99, 242, 313, 350, 842, 941
AjnI CCWGG 3 cut(s) 84, 460, 730
AluBI AGCT 3 cut(s) 185, 308, 417
AluI AGCT 3 cut(s) 185, 308, 417
Alw21I GWGCWC 1 cut(s) 531
Alw44I GTGCAC 1 cut(s) 527
AlwI GGATC 2 cut(s) 42, 839
Ama87I CYCGRG 2 cut(s) 338, 377
AoxI GGCC 1 cut(s) 154
ApaLI GTGCAC 1 cut(s) 527
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 2 cut(s) 255, 955
AspS9I GGNCC 3 cut(s) 452, 505, 734
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 1 cut(s) 475
AsuII TTCGAA 1 cut(s) 253
AvaI CYCGRG 2 cut(s) 338, 377
AvaII GGWCC 3 cut(s) 452, 505, 734
BaeGI GKGCMC 1 cut(s) 531
BanI GGYRCC 1 cut(s) 88
BauI CACGAG 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 1 cut(s) 641
BccI CCATC 2 cut(s) 695, 773
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 3 cut(s) 86, 462, 732
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 1 cut(s) 503
BfaI CTAG 1 cut(s) 961
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 623
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 4 cut(s) 86, 462, 503, 732
Bme18I GGWCC 3 cut(s) 452, 505, 734
BmeT110I CYCGRG 2 cut(s) 338, 377
BmgT120I GGNCC 3 cut(s) 452, 505, 734
BmiI GGNNCC 4 cut(s) 90, 210, 459, 507
BmrFI CCNGG 4 cut(s) 86, 462, 503, 732
BmsI GCATC 4 cut(s) 682, 704, 814, 890
BpmI CTGGAG 1 cut(s) 714
Bpu14I TTCGAA 1 cut(s) 253
BpuMI CCSGG 1 cut(s) 503
BsaAI YACGTR 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 706
BsaJI CCNNGG 5 cut(s) 84, 195, 339, 501, 859
BsaWI WCCGGW 2 cut(s) 454, 736
BsaXI ACNNNNNCTCC 2 cut(s) 586, 616
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 461, 803
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 3 cut(s) 86, 462, 732
BseDI CCNNGG 5 cut(s) 84, 195, 339, 501, 859
BseGI GGATG 2 cut(s) 697, 706
BseLI CCNNNNNNNGG 3 cut(s) 24, 461, 803
BseNI ACTGG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 1 cut(s) 641
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 156
BshNI GGYRCC 1 cut(s) 88
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 531
BsiHKCI CYCGRG 2 cut(s) 338, 377
BsiSI CCGG 3 cut(s) 455, 503, 737
BslFI GGGAC 1 cut(s) 408
BslI CCNNNNNNNGG 3 cut(s) 24, 461, 803
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 1 cut(s) 156
BsoBI CYCGRG 2 cut(s) 338, 377
Bsp119I TTCGAA 1 cut(s) 253
Bsp1286I GDGCHC 1 cut(s) 531
Bsp143I GATC 5 cut(s) 47, 265, 364, 775, 844
BspACI CCGC 5 cut(s) 218, 510, 558, 689, 827
BspANI GGCC 1 cut(s) 156
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 4 cut(s) 90, 210, 459, 507
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 2 cut(s) 42, 839
BspQI GCTCTTC 1 cut(s) 878
BspT104I TTCGAA 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 88
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 5 cut(s) 84, 195, 339, 501, 859
BssMI GATC 5 cut(s) 47, 265, 364, 775, 844
BssNI GRCGYC 1 cut(s) 706
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 859
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 3 cut(s) 86, 462, 732
Bst4CI ACNGT 2 cut(s) 235, 927
Bst6I CTCTTC 1 cut(s) 878
BstACI GRCGYC 1 cut(s) 706
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 253
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 2 cut(s) 697, 706
BstKTI GATC 5 cut(s) 50, 268, 367, 778, 847
BstMBI GATC 5 cut(s) 47, 265, 364, 775, 844
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstNI CCWGG 3 cut(s) 86, 462, 732
BstSCI CCNGG 4 cut(s) 84, 460, 501, 730
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 531
BstV1I GCAGC 1 cut(s) 641
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
BsuRI GGCC 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 2 cut(s) 697, 706
BveI ACCTGC 1 cut(s) 623
CciI TCATGA 1 cut(s) 66
Cfr13I GGNCC 3 cut(s) 452, 505, 734
Csp6I GTAC 3 cut(s) 221, 673, 836
CviAII CATG 1 cut(s) 67
CviJI RGCY 6 cut(s) 156, 185, 308, 323, 356, 417
CviKI_1 RGCY 6 cut(s) 156, 185, 308, 323, 356, 417
CviQI GTAC 3 cut(s) 221, 673, 836
DdeI CTNAG 1 cut(s) 516
DpnI GATC 5 cut(s) 49, 267, 366, 777, 846
DpnII GATC 5 cut(s) 47, 265, 364, 775, 844
EaeI YGGCCR 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 878
EarI CTCTTC 1 cut(s) 878
Eco130I CCWWGG 1 cut(s) 859
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 3 cut(s) 452, 505, 734
Eco88I CYCGRG 2 cut(s) 338, 377
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 3 cut(s) 84, 460, 730
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 859
EcoT22I ATGCAT 2 cut(s) 106, 697
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 1 cut(s) 70
FalI AAGNNNNNCTT 2 cut(s) 207, 239
FaqI GGGAC 1 cut(s) 408
FatI CATG 1 cut(s) 66
FauI CCCGC 2 cut(s) 517, 551
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 2 cut(s) 704, 713
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 1 cut(s) 961
GluI GCNGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 1 cut(s) 156
HapII CCGG 3 cut(s) 455, 503, 737
Hin1I GRCGYC 1 cut(s) 706
Hin1II CATG 1 cut(s) 70
HinfI GANTC 1 cut(s) 761
HpaII CCGG 3 cut(s) 455, 503, 737
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 7 cut(s) 330, 414, 468, 579, 700, 754, 949
Hpy188III TCNNGA 5 cut(s) 67, 227, 362, 587, 842
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 2 cut(s) 564, 928
HpyCH4III ACNGT 2 cut(s) 235, 927
HpyCH4IV ACGT 2 cut(s) 706, 853
HpyCH4V TGCA 6 cut(s) 4, 104, 119, 271, 529, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 706, 853
Hsp92I GRCGYC 1 cut(s) 706
Hsp92II CATG 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 5 cut(s) 47, 265, 364, 775, 844
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 2 cut(s) 305, 594
Lsp1109I GCAGC 1 cut(s) 641
LweI GCATC 4 cut(s) 682, 704, 814, 890
MaeI CTAG 1 cut(s) 961
MaeII ACGT 2 cut(s) 706, 853
MaeIII GTNAC 1 cut(s) 602
MalI GATC 5 cut(s) 49, 267, 366, 777, 846
MboI GATC 5 cut(s) 47, 265, 364, 775, 844
MboII GAAGA 1 cut(s) 865
MfeI CAATTG 2 cut(s) 172, 941
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 1 cut(s) 531
MluCI AATT 5 cut(s) 172, 255, 350, 941, 955
MnlI CCTC 5 cut(s) 199, 334, 369, 738, 748
Mph1103I ATGCAT 2 cut(s) 106, 697
MseI TTAA 4 cut(s) 384, 536, 785, 915
MspI CCGG 3 cut(s) 455, 503, 737
MspR9I CCNGG 4 cut(s) 86, 462, 503, 732
MunI CAATTG 2 cut(s) 172, 941
MvaI CCWGG 3 cut(s) 86, 462, 732
MwoI GCNNNNNNNGC 1 cut(s) 629
NciI CCSGG 1 cut(s) 503
NdeII GATC 5 cut(s) 47, 265, 364, 775, 844
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 4 cut(s) 90, 210, 459, 507
NsiI ATGCAT 2 cut(s) 106, 697
NspV TTCGAA 1 cut(s) 253
PagI TCATGA 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 878
PfeI GAWTC 1 cut(s) 761
PfoI TCCNGGA 1 cut(s) 730
PkrI GCNGC 1 cut(s) 631
Ple19I CGATCG 1 cut(s) 367
Ppu21I YACGTR 1 cut(s) 854
PsiI TTATAA 1 cut(s) 618
Psp6I CCWGG 3 cut(s) 84, 460, 730
PspGI CCWGG 3 cut(s) 84, 460, 730
PspN4I GGNNCC 4 cut(s) 90, 210, 459, 507
PspPI GGNCC 3 cut(s) 452, 505, 734
PsuI RGATCY 1 cut(s) 844
PvuI CGATCG 1 cut(s) 367
RsaI GTAC 3 cut(s) 222, 674, 837
RsaNI GTAC 3 cut(s) 221, 673, 836
SapI GCTCTTC 1 cut(s) 878
SaqAI TTAA 4 cut(s) 384, 536, 785, 915
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 5 cut(s) 47, 265, 364, 775, 844
Sau96I GGNCC 3 cut(s) 452, 505, 734
ScaI AGTACT 1 cut(s) 837
ScrFI CCNGG 4 cut(s) 86, 462, 503, 732
SduI GDGCHC 1 cut(s) 531
SfaNI GCATC 4 cut(s) 682, 704, 814, 890
SfcI CTRYAG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 253
SinI GGWCC 3 cut(s) 452, 505, 734
Sse9I AATT 5 cut(s) 172, 255, 350, 941, 955
SsiI CCGC 5 cut(s) 218, 510, 558, 689, 827
SspI AATATT 1 cut(s) 439
SspMI CTAG 1 cut(s) 961
StyD4I CCNGG 4 cut(s) 84, 460, 501, 730
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 2 cut(s) 235, 927
TaiI ACGT 2 cut(s) 709, 856
TaqI TCGA 3 cut(s) 46, 204, 253
TasI AATT 5 cut(s) 172, 255, 350, 941, 955
TatI WGTACW 1 cut(s) 835
TfiI GAWTC 1 cut(s) 761
Tru1I TTAA 4 cut(s) 384, 536, 785, 915
Tru9I TTAA 4 cut(s) 384, 536, 785, 915
TseI GCWGC 1 cut(s) 629
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 46
VneI GTGCAC 1 cut(s) 527
VpaK11BI GGWCC 3 cut(s) 452, 505, 734
XapI RAATTY 2 cut(s) 255, 955
XspI CTAG 1 cut(s) 961
ZraI GACGTC 1 cut(s) 707
ZrmI AGTACT 1 cut(s) 837
Zsp2I ATGCAT 2 cut(s) 106, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.