RCHIOBHM_CPG0502071

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0020230
Physical Location & Seq
Reverse (-)
48529 .. 50178
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15695

Sequence Viewer

Length: 963 bp
ATGCAAACTAAAAAAACCTTTTCGTCGATAAAGAAAGAGATTACTCGATCCATTTCCCTATCGCTCATGATATATATAATAACTCAGGCACCCATTTCAAATGCCTATCCCATTTTTGCACAGCAGGGTTATGAAAATCCACGAGAAGCAACGGGCCGTATTGTATGTGCCAATTGCCATTTAGCTAATAAACCCGTGGATATCGAGGTTCCACAAGCGGTACTTCCGGATACTGTATTTGAAGCAGTTGTTCGAATTCCCTATGATCTGCAAGTGAAACAAGTTCTTGCTAATGGTAAAAAAGGCGCTTTGAATGTGGGGGCTGTTCTTATTTTACCCGAGGGGTTTGAACTAGCCCCGCCCGATCGTATTTCGCCCGAGATTAAAGAAAAGATAGGAAATCTGTCTTTTCAAAGTTACCGCCCTACTAAAAAAAATATTCTTGTGATAGGTCCTGTTCCTGGTCAGAAATATAGTGAAATCACCTTTCCTATTCTTTCCCCGGACCCCGCTACTAAGAAAGATGTTCACTTCTTAAAATATCCCATATACGTAGGTGGGAACAGAGGAAGGGGTCAGATTTATCCCGACGGGAGCAAGAGTAACAATAATGTTTATAATGCTACAGCAGCAGGTATAGTAAGCAAAATCATACGAAAAGAAAAGGGGGGATACGAAATAACCATAGTGGAGGCATCGGGTGGGCGTCAAGTGGTTGATATTATCCCTCCAGGGCCGGAACTTCTTGTTTCTGAGGGCGAATCCATCAAACTTGATCAACCATTAACGAGTAATCCTAATGTGGGCGGATTTGGTCAGGGGGATGCAGAAGTAGTACTTCAAGATCCATTACGTGTCCAAGGCCTTTTGCTCTTCTTGGCATCTGTTATTTTTGCACAAATCTTTTTGGTTCTTAAAAAGAAACAGTTTGAGAAAGTTCAATTGTCCGAAATGAATTTCTAG

Protein Analysis

320

Amino Acids

35.0

Weight (kDa)

9.08

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Apocytochr_F_N PF16639 36 - 190 1.8e-79 Apocytochrome F, N-terminal
Apocytochr_F_C PF01333 202 - 320 5e-50 Apocytochrome F, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020230)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00540
malus_domestica MD09G1098000.v1.1 MD12G1014500.v1.1
pyrus_communis pycom12397g00220
rosa_chinensis RchiOBHm_CPg0502071
rosa_rugosa Rorug02G0490700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 1 cut(s) 88
AccIII TCCGGA 1 cut(s) 226
AciI CCGC 5 cut(s) 218, 359, 421, 510, 807
AclWI GGATC 2 cut(s) 42, 839
AcsI RAATTY 2 cut(s) 255, 955
AcyI GRCGYC 1 cut(s) 706
AfaI GTAC 2 cut(s) 222, 837
AfiI CCNNNNNNNGG 2 cut(s) 461, 803
AflIII ACRYGT 1 cut(s) 853
AgsI TTSAA 7 cut(s) 99, 242, 313, 350, 413, 842, 941
AjnI CCWGG 2 cut(s) 460, 730
AluBI AGCT 1 cut(s) 185
AluI AGCT 1 cut(s) 185
AlwI GGATC 2 cut(s) 42, 839
Ama87I CYCGRG 2 cut(s) 338, 377
Aor13HI TCCGGA 1 cut(s) 226
AoxI GGCC 3 cut(s) 154, 734, 862
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 2 cut(s) 255, 955
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 4 cut(s) 154, 452, 505, 734
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 1 cut(s) 475
AsuII TTCGAA 1 cut(s) 253
AvaI CYCGRG 2 cut(s) 338, 377
AvaII GGWCC 2 cut(s) 452, 505
BanI GGYRCC 1 cut(s) 88
BauI CACGAG 1 cut(s) 141
BbvI GCAGC 1 cut(s) 641
BccI CCATC 1 cut(s) 773
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 2 cut(s) 462, 732
BciVI GTATCC 2 cut(s) 223, 665
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 1 cut(s) 503
BfaI CTAG 2 cut(s) 353, 961
BfmI CTRYAG 1 cut(s) 624
BfoI RGCGCY 1 cut(s) 309
BfuAI ACCTGC 1 cut(s) 623
BfuI GTATCC 2 cut(s) 223, 665
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
BmcAI AGTACT 1 cut(s) 837
Bme1390I CCNGG 3 cut(s) 462, 503, 732
Bme18I GGWCC 2 cut(s) 452, 505
BmeT110I CYCGRG 2 cut(s) 338, 377
BmgT120I GGNCC 4 cut(s) 154, 452, 505, 734
BmiI GGNNCC 3 cut(s) 90, 210, 507
BmrFI CCNGG 3 cut(s) 462, 503, 732
BmsI GCATC 3 cut(s) 704, 814, 890
BpmI CTGGAG 1 cut(s) 714
Bpu14I TTCGAA 1 cut(s) 253
BpuMI CCSGG 1 cut(s) 503
BsaAI YACGTR 2 cut(s) 553, 854
BsaHI GRCGYC 1 cut(s) 706
BsaJI CCNNGG 5 cut(s) 195, 339, 501, 731, 859
BsaWI WCCGGW 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 586, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 803
BseAI TCCGGA 1 cut(s) 226
BseBI CCWGG 2 cut(s) 462, 732
BseDI CCNNGG 5 cut(s) 195, 339, 501, 731, 859
BseGI GGATG 1 cut(s) 829
BseLI CCNNNNNNNGG 2 cut(s) 461, 803
BseMII CTCAG 2 cut(s) 98, 744
BseXI GCAGC 1 cut(s) 641
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 3 cut(s) 156, 736, 864
BshNI GGYRCC 1 cut(s) 88
BsiEI CGRYCG 1 cut(s) 367
BsiHKCI CYCGRG 2 cut(s) 338, 377
BsiSI CCGG 3 cut(s) 227, 503, 737
BslI CCNNNNNNNGG 2 cut(s) 461, 803
BsnI GGCC 3 cut(s) 156, 736, 864
BsoBI CYCGRG 2 cut(s) 338, 377
Bsp119I TTCGAA 1 cut(s) 253
Bsp13I TCCGGA 1 cut(s) 226
Bsp143I GATC 5 cut(s) 47, 265, 364, 775, 844
BspACI CCGC 5 cut(s) 218, 359, 421, 510, 807
BspANI GGCC 3 cut(s) 156, 736, 864
BspCNI CTCAG 2 cut(s) 97, 745
BspEI TCCGGA 1 cut(s) 226
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 90, 210, 507
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 2 cut(s) 42, 839
BspQI GCTCTTC 1 cut(s) 878
BspT104I TTCGAA 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 88
BssECI CCNNGG 5 cut(s) 195, 339, 501, 731, 859
BssMI GATC 5 cut(s) 47, 265, 364, 775, 844
BssNI GRCGYC 1 cut(s) 706
BssSI CACGAG 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 859
Bst2BI CACGAG 1 cut(s) 141
Bst2UI CCWGG 2 cut(s) 462, 732
Bst4CI ACNGT 2 cut(s) 235, 927
Bst6I CTCTTC 1 cut(s) 878
BstACI GRCGYC 1 cut(s) 706
BstBAI YACGTR 2 cut(s) 553, 854
BstBI TTCGAA 1 cut(s) 253
BstDEI CTNAG 3 cut(s) 84, 516, 753
BstDSI CCRYGG 1 cut(s) 195
BstF5I GGATG 1 cut(s) 829
BstH2I RGCGCY 1 cut(s) 309
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 5 cut(s) 50, 268, 367, 778, 847
BstMBI GATC 5 cut(s) 47, 265, 364, 775, 844
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstNI CCWGG 2 cut(s) 462, 732
BstSCI CCNGG 3 cut(s) 460, 501, 730
BstSFI CTRYAG 1 cut(s) 624
BstSNI TACGTA 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 641
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
BsuI GTATCC 2 cut(s) 223, 665
BsuRI GGCC 3 cut(s) 156, 736, 864
BtgI CCRYGG 1 cut(s) 195
BtsCI GGATG 1 cut(s) 829
BveI ACCTGC 1 cut(s) 623
CciI TCATGA 1 cut(s) 66
CfoI GCGC 1 cut(s) 308
Cfr13I GGNCC 4 cut(s) 154, 452, 505, 734
CseI GACGC 1 cut(s) 695
Csp6I GTAC 2 cut(s) 221, 836
CviAII CATG 1 cut(s) 67
CviJI RGCY 6 cut(s) 156, 185, 323, 356, 736, 864
CviKI_1 RGCY 6 cut(s) 156, 185, 323, 356, 736, 864
CviQI GTAC 2 cut(s) 221, 836
DdeI CTNAG 3 cut(s) 84, 516, 753
DpnI GATC 5 cut(s) 49, 267, 366, 777, 846
DpnII GATC 5 cut(s) 47, 265, 364, 775, 844
Eam1104I CTCTTC 1 cut(s) 878
EarI CTCTTC 1 cut(s) 878
EciI GGCGGA 1 cut(s) 822
Eco105I TACGTA 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 859
Eco147I AGGCCT 1 cut(s) 864
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 452, 505
Eco88I CYCGRG 2 cut(s) 338, 377
EcoO109I RGGNCCY 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 2 cut(s) 460, 730
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 859
ErhI CCWWGG 1 cut(s) 859
FaeI CATG 1 cut(s) 70
FalI AAGNNNNNCTT 4 cut(s) 207, 239, 822, 854
FatI CATG 1 cut(s) 66
FauI CCCGC 2 cut(s) 366, 517
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 1 cut(s) 836
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 2 cut(s) 353, 961
GlaI GCGC 1 cut(s) 307
GluI GCNGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 714
HaeII RGCGCY 1 cut(s) 309
HaeIII GGCC 3 cut(s) 156, 736, 864
HapII CCGG 3 cut(s) 227, 503, 737
HgaI GACGC 1 cut(s) 695
HhaI GCGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 706
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HinfI GANTC 1 cut(s) 761
HpaII CCGG 3 cut(s) 227, 503, 737
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 4 cut(s) 468, 579, 754, 949
Hpy188III TCNNGA 4 cut(s) 67, 227, 587, 842
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 2 cut(s) 28, 593
HpyAV CCTTC 1 cut(s) 564
HpyCH4III ACNGT 2 cut(s) 235, 927
HpyCH4IV ACGT 2 cut(s) 552, 853
HpyCH4V TGCA 5 cut(s) 4, 119, 271, 827, 896
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpyF3I CTNAG 3 cut(s) 84, 516, 753
HpySE526I ACGT 2 cut(s) 552, 853
Hsp92I GRCGYC 1 cut(s) 706
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 306
Kpn2I TCCGGA 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 5 cut(s) 47, 265, 364, 775, 844
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 1 cut(s) 594
Lsp1109I GCAGC 1 cut(s) 641
LweI GCATC 3 cut(s) 704, 814, 890
MaeI CTAG 2 cut(s) 353, 961
MaeII ACGT 2 cut(s) 552, 853
MaeIII GTNAC 2 cut(s) 416, 602
MalI GATC 5 cut(s) 49, 267, 366, 777, 846
MboI GATC 5 cut(s) 47, 265, 364, 775, 844
MboII GAAGA 1 cut(s) 865
MfeI CAATTG 2 cut(s) 172, 941
MflI RGATCY 1 cut(s) 844
MluCI AATT 4 cut(s) 172, 255, 941, 955
MnlI CCTC 6 cut(s) 199, 334, 560, 685, 738, 748
MroI TCCGGA 1 cut(s) 226
MseI TTAA 4 cut(s) 384, 536, 785, 915
MspI CCGG 3 cut(s) 227, 503, 737
MspR9I CCNGG 3 cut(s) 462, 503, 732
MunI CAATTG 2 cut(s) 172, 941
MvaI CCWGG 2 cut(s) 462, 732
MwoI GCNNNNNNNGC 1 cut(s) 629
NciI CCSGG 1 cut(s) 503
NdeII GATC 5 cut(s) 47, 265, 364, 775, 844
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 3 cut(s) 90, 210, 507
NspV TTCGAA 1 cut(s) 253
PagI TCATGA 1 cut(s) 66
PceI AGGCCT 1 cut(s) 864
PciSI GCTCTTC 1 cut(s) 878
PfeI GAWTC 1 cut(s) 761
PkrI GCNGC 1 cut(s) 631
Ple19I CGATCG 1 cut(s) 367
Ppu21I YACGTR 2 cut(s) 553, 854
PpuMI RGGWCCY 1 cut(s) 452
PsiI TTATAA 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 2 cut(s) 460, 730
PspGI CCWGG 2 cut(s) 460, 730
PspN4I GGNNCC 3 cut(s) 90, 210, 507
PspPI GGNCC 4 cut(s) 154, 452, 505, 734
PspPPI RGGWCCY 1 cut(s) 452
PsuI RGATCY 1 cut(s) 844
PvuI CGATCG 1 cut(s) 367
RsaI GTAC 2 cut(s) 222, 837
RsaNI GTAC 2 cut(s) 221, 836
SapI GCTCTTC 1 cut(s) 878
SaqAI TTAA 4 cut(s) 384, 536, 785, 915
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 5 cut(s) 47, 265, 364, 775, 844
Sau96I GGNCC 4 cut(s) 154, 452, 505, 734
ScaI AGTACT 1 cut(s) 837
ScrFI CCNGG 3 cut(s) 462, 503, 732
SetI ASST 9 cut(s) 20, 187, 210, 454, 488, 555, 559, 637, 856
SfaNI GCATC 3 cut(s) 704, 814, 890
SfcI CTRYAG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 253
SinI GGWCC 2 cut(s) 452, 505
SnaBI TACGTA 1 cut(s) 553
Sse9I AATT 4 cut(s) 172, 255, 941, 955
SseBI AGGCCT 1 cut(s) 864
SsiI CCGC 5 cut(s) 218, 359, 421, 510, 807
SspI AATATT 1 cut(s) 439
SspMI CTAG 2 cut(s) 353, 961
StuI AGGCCT 1 cut(s) 864
StyD4I CCNGG 3 cut(s) 460, 501, 730
StyI CCWWGG 1 cut(s) 859
TaaI ACNGT 2 cut(s) 235, 927
TaiI ACGT 2 cut(s) 555, 856
TaqI TCGA 4 cut(s) 26, 46, 204, 253
TasI AATT 4 cut(s) 172, 255, 941, 955
TatI WGTACW 1 cut(s) 835
TfiI GAWTC 1 cut(s) 761
Tru1I TTAA 4 cut(s) 384, 536, 785, 915
Tru9I TTAA 4 cut(s) 384, 536, 785, 915
TseI GCWGC 1 cut(s) 629
TspDTI ATGAA 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 452, 505
XapI RAATTY 2 cut(s) 255, 955
XspI CTAG 2 cut(s) 353, 961
ZrmI AGTACT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.