pycom12g00920
ERF Family

DnaJ Hsp40 cysteine-rich domain superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014243
Physical Location & Seq
Forward (+)
803903 .. 805876
1974 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGGGTTCTGCTTCTGCTGCTGCAAATATGGCGTCTTTGATTACTTTCGTCCCTGTTTCTTCGACAAGATTGAGTGTCCATGACAAGTTTTCATTTGGGTTTCATCACAAACGTGGTGGGTCGATCGCCAAATTTTCTGCCGCCATCTCTCCCAACGGCTCTGTTTCTCCTTCTTCTTCACATGGGTCTGGGTGCTCAGCTGGAGATGCGGATAAACAAAGAAGTAGTCTTGCATCTCTGTTCTGTTATGACAAGTCTGTACCGGAAGAGAGAATTGAGAAGCCTGTTGGGATATCCTTGACTGAGAAATTCATTGGAAACAAACCACGATGCATTGATTGTGAAGCCAAAGGTGCAGTCCTTTGCACCACTTGTTCTGGTTCAGGCCTGTATGTTGATTCAATATTGGAGAGCCAGGGTATTATTGTCAAAGTTCGCTGCCTAGGTTGCGGGGGAAGTGGCAACATTATGTGTGGAGAATGTGGTGGCCGCGGGCATCGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

17.25

Weight (kDa)

8.72

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD2_CRD PF25436 111 - 168 2.2e-06 BSD2 cysteine rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014243)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17840
fragaria_vesca FvH4_1g29880
malus_domestica MD12G1011900.v1.1 MD14G1009500.v1.1
prunus_persica Prupe.7G015800_v2.0.a1
pyrus_communis pycom12g00920
rosa_chinensis RchiOBHm_Chr3g0496031
rosa_laevigata RLG00000022571
rosa_multiflora Rmu_sc0000607.1_g000055 Rmu_sc0005781.1_g000028
rosa_roxburghii Rroxscaffold_6G00389640
rosa_rugosa Rorug03G0279200
rosa_samantha Rh3AG325900 Rh3BG362300 Rh3CG358300 Rh3DG361700
rosa_wichuraiana Rw3G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 492
AciI CCGC 5 cut(s) 141, 209, 450, 490, 492
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 2 cut(s) 131, 308
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 1 cut(s) 261
AgsI TTSAA 1 cut(s) 402
AjnI CCWGG 1 cut(s) 414
AleI CACNNNNGTG 1 cut(s) 111
AluBI AGCT 1 cut(s) 200
AluI AGCT 1 cut(s) 200
Alw21I GWGCWC 1 cut(s) 197
AoxI GGCC 2 cut(s) 385, 487
ApeKI GCWGC 3 cut(s) 17, 20, 438
ApoI RAATTY 2 cut(s) 131, 308
ArsI GACNNNNNNTTYG 6 cut(s) 17, 49, 211, 243, 342, 374
AspA2I CCTAGG 1 cut(s) 442
AvrII CCTAGG 1 cut(s) 442
Bbv12I GWGCWC 1 cut(s) 197
BbvI GCAGC 3 cut(s) 4, 7, 425
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 416
BfaI CTAG 1 cut(s) 443
BisI GCNGC 5 cut(s) 18, 21, 141, 439, 490
BlnI CCTAGG 1 cut(s) 442
BlpI GCTNAGC 1 cut(s) 196
BlsI GCNGC 5 cut(s) 19, 22, 142, 440, 491
Bme1390I CCNGG 1 cut(s) 416
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 3 cut(s) 196, 242, 320
BpmI CTGGAG 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 196
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 415, 442, 490
BsaWI WCCGGW 1 cut(s) 262
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 3 cut(s) 415, 442, 490
BseMII CTCAG 2 cut(s) 210, 294
BseXI GCAGC 3 cut(s) 4, 7, 425
BsgI GTGCAG 1 cut(s) 375
Bsh1236I CGCG 1 cut(s) 492
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 387, 489
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 2 cut(s) 387, 489
Bsp1286I GDGCHC 1 cut(s) 197
Bsp143I GATC 1 cut(s) 123
Bsp1720I GCTNAGC 1 cut(s) 196
BspACI CCGC 5 cut(s) 141, 209, 450, 490, 492
BspANI GGCC 2 cut(s) 387, 489
BspCNI CTCAG 2 cut(s) 209, 295
BspFNI CGCG 1 cut(s) 492
BssECI CCNNGG 3 cut(s) 415, 442, 490
BssMI GATC 1 cut(s) 123
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 416
Bst6I CTCTTC 1 cut(s) 261
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 2 cut(s) 196, 303
BstDSI CCRYGG 1 cut(s) 490
BstFNI CGCG 1 cut(s) 492
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 17, 29, 206, 353, 447
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 414
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 3 cut(s) 4, 7, 425
BsuRI GGCC 2 cut(s) 387, 489
BtgI CCRYGG 1 cut(s) 490
Cac8I GCNNGC 1 cut(s) 494
Cfr42I CCGCGG 1 cut(s) 493
CseI GACGC 1 cut(s) 21
Csp6I GTAC 1 cut(s) 260
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 2 cut(s) 80, 182
CviJI RGCY 7 cut(s) 159, 200, 283, 347, 387, 414, 489
CviKI_1 RGCY 7 cut(s) 159, 200, 283, 347, 387, 414, 489
CviQI GTAC 1 cut(s) 260
DdeI CTNAG 2 cut(s) 196, 303
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
EaeI YGGCCR 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 442
Eco147I AGGCCT 1 cut(s) 387
Eco32I GATATC 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 414
EcoRV GATATC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 2 cut(s) 83, 185
FaiI YATR 6 cut(s) 29, 81, 183, 249, 393, 470
FaqI GGGAC 1 cut(s) 35
FatI CATG 2 cut(s) 79, 181
FauI CCCGC 2 cut(s) 443, 485
Fnu4HI GCNGC 5 cut(s) 18, 21, 141, 439, 490
Fsp4HI GCNGC 5 cut(s) 18, 21, 141, 439, 490
FspBI CTAG 1 cut(s) 443
GluI GCNGC 5 cut(s) 18, 21, 141, 439, 490
GsuI CTGGAG 1 cut(s) 222
HaeIII GGCC 2 cut(s) 387, 489
HapII CCGG 1 cut(s) 263
HgaI GACGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 2 cut(s) 83, 185
HinfI GANTC 1 cut(s) 398
HpaII CCGG 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 180
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 5 cut(s) 23, 233, 333, 356, 366
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 29, 206, 353, 447
HpyF3I CTNAG 2 cut(s) 196, 303
HpySE526I ACGT 1 cut(s) 112
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 2 cut(s) 83, 185
KspI CCGCGG 1 cut(s) 493
Kzo9I GATC 1 cut(s) 123
Lsp1109I GCAGC 3 cut(s) 4, 7, 425
LweI GCATC 3 cut(s) 196, 242, 320
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 112
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 4 cut(s) 51, 165, 168, 278
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 3 cut(s) 131, 273, 308
MnlI CCTC 1 cut(s) 494
Mph1103I ATGCAT 1 cut(s) 335
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 200, 492
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 5 cut(s) 17, 29, 206, 353, 447
NdeII GATC 1 cut(s) 123
NlaIII CATG 2 cut(s) 83, 185
NsiI ATGCAT 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 111
PceI AGGCCT 1 cut(s) 387
PfeI GAWTC 1 cut(s) 398
PkrI GCNGC 5 cut(s) 19, 22, 142, 440, 491
Ple19I CGATCG 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PvuI CGATCG 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 261
RsaNI GTAC 1 cut(s) 260
RseI CAYNNNNRTG 1 cut(s) 111
SacII CCGCGG 1 cut(s) 493
SatI GCNGC 5 cut(s) 18, 21, 141, 439, 490
Sau3AI GATC 1 cut(s) 123
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 197
SetI ASST 4 cut(s) 115, 202, 355, 448
SfaNI GCATC 3 cut(s) 196, 242, 320
Sfr303I CCGCGG 1 cut(s) 493
SgrBI CCGCGG 1 cut(s) 493
SmiMI CAYNNNNRTG 1 cut(s) 111
Sse9I AATT 3 cut(s) 131, 273, 308
SseBI AGGCCT 1 cut(s) 387
SsiI CCGC 5 cut(s) 141, 209, 450, 490, 492
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 443
StuI AGGCCT 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 442
TaiI ACGT 1 cut(s) 115
TaqI TCGA 3 cut(s) 62, 122, 499
TasI AATT 3 cut(s) 131, 273, 308
TauI GCSGC 2 cut(s) 143, 492
TfiI GAWTC 1 cut(s) 398
TseI GCWGC 3 cut(s) 17, 20, 438
TspDTI ATGAA 3 cut(s) 81, 92, 301
XapI RAATTY 2 cut(s) 131, 308
XmaJI CCTAGG 1 cut(s) 442
XspI CTAG 1 cut(s) 443
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.