pycom12g04860

PMR5 N terminal Domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0021279
Physical Location & Seq
Forward (+)
4789909 .. 4790160
252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04860.1

Sequence Viewer

Length: 252 bp
ATGAAAACCCATGTCACATATCTCAATATTACAAGAATGACAGATTTTCGGAAGGATGGCCACCCATCAATCTACCGGAAGCAAAAACTGTCCGAGGAAGAAAGGAGATCACCTACAAGCTTCCAAGACTGCAGCCATTGGTGCCTTCCTGGTGTTCCAGACGCTTGGAATGAGGTTCTCTATGCCGAGCTCCTGGTAAAGCTGTACCAAAATAAGCAGCAGCTACTGCAACAGCAGAAGAAGAGAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

84

Amino Acids

9.95

Weight (kDa)

9.43

Isoelectric Point (pI)

73.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 1 - 62 3.2e-25 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021279)

Species Orthologous Gene IDs
pyrus_communis pycom12g04860
rosa_chinensis RchiOBHm_Chr3g0462711
rosa_roxburghii Rroxscaffold_6G00417260
rosa_samantha Rh3BG120500 Rh4DG328900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 58
AfaI GTAC 1 cut(s) 206
AjnI CCWGG 2 cut(s) 148, 192
AluBI AGCT 4 cut(s) 120, 190, 202, 223
AluI AGCT 4 cut(s) 120, 190, 202, 223
Alw21I GWGCWC 1 cut(s) 192
AlwNI CAGNNNCTG 1 cut(s) 226
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 3 cut(s) 132, 217, 220
AsuHPI GGTGA 1 cut(s) 102
BalI TGGCCA 1 cut(s) 60
BanI GGYRCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 144, 229, 232
BccI CCATC 2 cut(s) 50, 73
BciT130I CCWGG 2 cut(s) 150, 194
BfmI CTRYAG 1 cut(s) 130
BisI GCNGC 3 cut(s) 133, 218, 221
BlsI GCNGC 3 cut(s) 134, 219, 222
Bme1390I CCNGG 2 cut(s) 150, 194
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 150, 194
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 75
BseBI CCWGG 2 cut(s) 150, 194
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 61
BseXI GCAGC 3 cut(s) 144, 229, 232
BshFI GGCC 1 cut(s) 60
BshNI GGYRCC 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 76
BsnI GGCC 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 1 cut(s) 107
BspANI GGCC 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 143
BspMAI CTGCAG 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 150, 194
Bst4CI ACNGT 1 cut(s) 90
Bst6I CTCTTC 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 226
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 1 cut(s) 110
BstMBI GATC 1 cut(s) 107
BstMWI GCNNNNNNNGC 2 cut(s) 141, 226
BstNI CCWGG 2 cut(s) 150, 194
BstSCI CCNGG 2 cut(s) 148, 192
BstSFI CTRYAG 1 cut(s) 130
BstV1I GCAGC 3 cut(s) 144, 229, 232
BstXI CCANNNNNNTGG 1 cut(s) 165
BsuRI GGCC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 61
CaiI CAGNNNCTG 1 cut(s) 226
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 11
CviJI RGCY 6 cut(s) 60, 120, 135, 190, 202, 223
CviKI_1 RGCY 6 cut(s) 60, 120, 135, 190, 202, 223
CviQI GTAC 1 cut(s) 205
DpnI GATC 1 cut(s) 109
DpnII GATC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 148, 192
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 1 cut(s) 14
FaiI YATR 4 cut(s) 12, 19, 183, 250
FatI CATG 1 cut(s) 10
Fnu4HI GCNGC 3 cut(s) 133, 218, 221
FokI GGATG 1 cut(s) 68
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 3 cut(s) 133, 218, 221
GluI GCNGC 3 cut(s) 133, 218, 221
HaeIII GGCC 1 cut(s) 60
HapII CCGG 1 cut(s) 76
HgaI GACGC 1 cut(s) 170
Hin1II CATG 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 118
HpaII CCGG 1 cut(s) 76
HphI GGTGA 1 cut(s) 102
Hpy188I TCNGA 2 cut(s) 51, 94
Hpy188III TCNNGA 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 46, 155
HpyCH4III ACNGT 1 cut(s) 90
HpyCH4V TGCA 2 cut(s) 132, 229
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 226
Hsp92II CATG 1 cut(s) 14
Kzo9I GATC 1 cut(s) 107
LmnI GCTCC 1 cut(s) 195
LpnPI CCDG 6 cut(s) 89, 135, 162, 171, 179, 206
Lsp1109I GCAGC 3 cut(s) 144, 229, 232
MaeIII GTNAC 1 cut(s) 13
MalI GATC 1 cut(s) 109
MboI GATC 1 cut(s) 107
MboII GAAGA 2 cut(s) 110, 250
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 60
MluNI TGGCCA 1 cut(s) 60
MnlI CCTC 2 cut(s) 88, 166
Mox20I TGGCCA 1 cut(s) 60
MscI TGGCCA 1 cut(s) 60
Msp20I TGGCCA 1 cut(s) 60
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 150, 194
MvaI CCWGG 2 cut(s) 150, 194
MwoI GCNNNNNNNGC 2 cut(s) 141, 226
NdeII GATC 1 cut(s) 107
NlaIII CATG 1 cut(s) 14
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 13
PkrI GCNGC 3 cut(s) 134, 219, 222
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 148, 192
PspGI CCWGG 2 cut(s) 148, 192
PspN4I GGNNCC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 226
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SacI GAGCTC 1 cut(s) 192
SatI GCNGC 3 cut(s) 133, 218, 221
Sau3AI GATC 1 cut(s) 107
ScrFI CCNGG 2 cut(s) 150, 194
SduI GDGCHC 1 cut(s) 192
SetI ASST 6 cut(s) 115, 122, 177, 192, 204, 225
SfcI CTRYAG 1 cut(s) 130
SspI AATATT 1 cut(s) 28
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 2 cut(s) 148, 192
TaaI ACNGT 1 cut(s) 90
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 3 cut(s) 132, 217, 220
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.