Rh4DG328900

GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0021279
Physical Location & Seq
Reverse (-)
55230091 .. 55230611
521 bp
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UTR
Exon/CDS
Intron
Rh4DG328900.1

Sequence Viewer

Length: 399 bp
ATGAACATCAATCCCAACCGGACTCGGGTCTTCTTCCGAGGATATGCATATTCCCATTACAGTAATGGGCAATGGAACTCAGGAGGACATTGCCTCAAAGAAACAGAACCAATCTTTAACGAAGCTCATTTATCGAAGTATCCTTCGAAAATGAGAGCTGTAGAGTCTGTACTTCAAGGAATGAAAACTCCGGTGACGTATTTAAACATAAGTAGGCTTACAGATTGCAGAAAAGATGCCCACCCTTCCATTTACAGAATGGAATACAAGACAAAAGAAGAAGAAATTGCTGCTGAACGATCTCAGGATTGCAACCATTGGTGCTTGCCGGGTGTTCCGGATACTTGGAACGAATTGCTATATGCCTCGTTTTTGAAGGACGGATGGGAATCAGGCTAG

Protein Analysis

132

Amino Acids

15.34

Weight (kDa)

6.95

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 2 - 122 2.5e-41 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021279)

Species Orthologous Gene IDs
pyrus_communis pycom12g04860
rosa_chinensis RchiOBHm_Chr3g0462711
rosa_roxburghii Rroxscaffold_6G00417260
rosa_samantha Rh3BG120500 Rh4DG328900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 337
AfaI GTAC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 25
AgsI TTSAA 2 cut(s) 176, 376
AluBI AGCT 2 cut(s) 125, 158
AluI AGCT 2 cut(s) 125, 158
Ama87I CYCGRG 1 cut(s) 24
Aor13HI TCCGGA 1 cut(s) 337
ApeKI GCWGC 1 cut(s) 290
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 1 cut(s) 205
AsuII TTCGAA 1 cut(s) 146
AvaI CYCGRG 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 22
BbvI GCAGC 1 cut(s) 277
BccI CCATC 1 cut(s) 378
BciVI GTATCC 2 cut(s) 150, 334
BcnI CCSGG 1 cut(s) 330
BfaI CTAG 1 cut(s) 397
BfmI CTRYAG 1 cut(s) 159
BfuI GTATCC 2 cut(s) 150, 334
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 330
BmeT110I CYCGRG 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 1 cut(s) 226
BoxI GACNNNNGTC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 22
Bpu14I TTCGAA 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 37
BsaWI WCCGGW 3 cut(s) 18, 190, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 25
Bse3DI GCAATG 2 cut(s) 77, 88
Bse8I GATNNNNATC 1 cut(s) 388
BseAI TCCGGA 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 37
BseGI GGATG 1 cut(s) 389
BseJI GATNNNNATC 1 cut(s) 388
BseLI CCNNNNNNNGG 1 cut(s) 25
BseMI GCAATG 2 cut(s) 77, 88
BseMII CTCAG 2 cut(s) 93, 317
BseXI GCAGC 1 cut(s) 277
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 4 cut(s) 19, 191, 329, 338
BslI CCNNNNNNNGG 1 cut(s) 25
BsoBI CYCGRG 1 cut(s) 24
Bsp119I TTCGAA 1 cut(s) 146
Bsp13I TCCGGA 1 cut(s) 337
Bsp143I GATC 1 cut(s) 299
BspCNI CTCAG 2 cut(s) 92, 316
BspEI TCCGGA 1 cut(s) 337
BspT104I TTCGAA 1 cut(s) 146
BsrDI GCAATG 2 cut(s) 77, 88
BssECI CCNNGG 1 cut(s) 37
BssMI GATC 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 62
BstBI TTCGAA 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 79, 303
BstF5I GGATG 1 cut(s) 389
BstKTI GATC 1 cut(s) 302
BstMBI GATC 1 cut(s) 299
BstPAI GACNNNNGTC 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 22
BsuI GTATCC 2 cut(s) 150, 334
BtsCI GGATG 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 170
CviJI RGCY 4 cut(s) 125, 158, 217, 396
CviKI_1 RGCY 4 cut(s) 125, 158, 217, 396
CviQI GTAC 1 cut(s) 170
DdeI CTNAG 2 cut(s) 79, 303
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
DraI TTTAAA 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 24
EcoT22I ATGCAT 1 cut(s) 49
FaiI YATR 5 cut(s) 45, 49, 209, 361, 363
Fnu4HI GCNGC 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 397
GluI GCNGC 1 cut(s) 291
HapII CCGG 4 cut(s) 19, 191, 329, 338
HinfI GANTC 3 cut(s) 22, 164, 389
HpaII CCGG 4 cut(s) 19, 191, 329, 338
HphI GGTGA 1 cut(s) 205
Hpy188I TCNGA 1 cut(s) 38
Hpy188III TCNNGA 3 cut(s) 81, 305, 338
HpyAV CCTTC 3 cut(s) 153, 255, 370
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 197
HpyCH4V TGCA 3 cut(s) 47, 228, 312
HpyF3I CTNAG 2 cut(s) 79, 303
HpySE526I ACGT 1 cut(s) 197
Kpn2I TCCGGA 1 cut(s) 337
Kzo9I GATC 1 cut(s) 299
LpnPI CCDG 7 cut(s) 32, 66, 204, 290, 342, 351, 378
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 226
MaeI CTAG 1 cut(s) 397
MaeII ACGT 1 cut(s) 197
MaeIII GTNAC 1 cut(s) 193
MalI GATC 1 cut(s) 301
MboI GATC 1 cut(s) 299
MboII GAAGA 4 cut(s) 22, 25, 290, 293
MluCI AATT 2 cut(s) 285, 353
MlyI GAGTC 2 cut(s) 16, 173
MnlI CCTC 4 cut(s) 32, 77, 104, 376
Mph1103I ATGCAT 1 cut(s) 49
MroI TCCGGA 1 cut(s) 337
MseI TTAA 2 cut(s) 117, 203
MspI CCGG 4 cut(s) 19, 191, 329, 338
MspR9I CCNGG 1 cut(s) 330
NciI CCSGG 1 cut(s) 330
NdeII GATC 1 cut(s) 299
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 49
NspV TTCGAA 1 cut(s) 146
PfeI GAWTC 1 cut(s) 389
PkrI GCNGC 1 cut(s) 292
PleI GAGTC 2 cut(s) 16, 172
PpsI GAGTC 2 cut(s) 16, 172
PshAI GACNNNNGTC 1 cut(s) 26
RsaI GTAC 1 cut(s) 171
RsaNI GTAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 117, 203
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 1 cut(s) 299
SchI GAGTC 2 cut(s) 16, 173
ScrFI CCNGG 1 cut(s) 330
SetI ASST 3 cut(s) 127, 160, 200
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 146
Sse9I AATT 2 cut(s) 285, 353
SspMI CTAG 1 cut(s) 397
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 1 cut(s) 200
TaqI TCGA 2 cut(s) 134, 146
TasI AATT 2 cut(s) 285, 353
TatI WGTACW 1 cut(s) 169
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 2 cut(s) 117, 203
Tru9I TTAA 2 cut(s) 117, 203
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 17, 197
TspGWI ACGGA 1 cut(s) 396
XcmI CCANNNNNNNNNTGG 2 cut(s) 62, 256
XspI CTAG 1 cut(s) 397
Zsp2I ATGCAT 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.