pycom12g12740

Putative zinc- or iron-chelating domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015510
Physical Location & Seq
Reverse (-)
15175203 .. 15176091
889 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12740.4

Sequence Viewer

Length: 513 bp
ATGTCGAACGCGGTGGCTTTGCACTGTCTTGGCATAGTGAGCATGGCGCGCCGGCCACGCCAGAAGGCCAAGGAAAAGAAAACCCGGGTCCCGAATCCGACACCGCCGACGAGTGTCGGGTTCGGAGCCAAAAGGAAGGAGCAGAAGTGGCAGTGCGTGGAGGGCTGCGGAGCTTGCTGCAAACTCGACAAAGGCCCCTCCTTTGCCACCCCGGAAGAGATTTTCACCAACCCTTCCGACATCGAGCTTTACAGAAGCATGGTCGGAGAAGATGGGTGGTGTGTGAATTTTGATAAAAGCACGCGAAAGTGTTCGATTTACGACGAACGTCCATATTTTTGCCGTGTGGAGGCAGAGGTGTTTCAGTCCTTGTATGGGTTTAGTGAGAAAAAATTCAACAAGGAGGCCTGCAGGAGCTGCAGGGACACAATTAAATCAGTTTACGGTCCCCATTCCAAAGAGTTGGTTAACTTCAAGAGTGCAGTAGAGAGCTCCAGCAGCACTACTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CxxCxxCC

Protein Analysis

171

Amino Acids

19.07

Weight (kDa)

9.0

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015510)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G02710 AT5G02710 AT5G02710
fragaria_vesca FvH4_6g14780 FvH4_6g14780
malus_domestica MD12G1131600.v1.1
prunus_persica Prupe.6G246000_v2.0.a1
pyrus_communis pycom12g12740
rosa_chinensis RchiOBHm_Chr3g0468011
rosa_laevigata RLG00000024460
rosa_multiflora Rmu_sc0006706.1_g000006
rosa_roxburghii Rroxscaffold_6G00413210
rosa_rugosa Rorug03G0091300 Rorug03G0091400
rosa_samantha Rh3BG161300
rosa_wichuraiana Rw3G013230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 11, 49, 304
AciI CCGC 3 cut(s) 11, 104, 168
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 2 cut(s) 286, 392
AfiI CCNNNNNNNGG 2 cut(s) 349, 375
AgsI TTSAA 2 cut(s) 397, 475
AhlI ACTAGT 1 cut(s) 506
AluBI AGCT 4 cut(s) 173, 247, 417, 492
AluI AGCT 4 cut(s) 173, 247, 417, 492
Alw21I GWGCWC 1 cut(s) 494
AlwNI CAGNNNCTG 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 84
AoxI GGCC 4 cut(s) 53, 66, 193, 405
ApeKI GCWGC 4 cut(s) 165, 177, 417, 498
ApoI RAATTY 2 cut(s) 286, 392
AscI GGCGCGCC 1 cut(s) 47
Asp700I GAANNNNTTC 2 cut(s) 310, 392
AspLEI GCGC 2 cut(s) 49, 51
AspS9I GGNCC 3 cut(s) 88, 194, 446
AsuC2I CCSGG 3 cut(s) 85, 86, 212
AsuHPI GGTGA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 2 cut(s) 88, 446
BanII GRGCYC 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 494
BbvI GCAGC 3 cut(s) 152, 164, 404
BccI CCATC 1 cut(s) 266
BceAI ACGGC 1 cut(s) 327
BcnI CCSGG 3 cut(s) 85, 86, 212
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 2 cut(s) 409, 418
BisI GCNGC 4 cut(s) 166, 178, 418, 499
BlsI GCNGC 4 cut(s) 167, 179, 419, 500
Bme1390I CCNGG 3 cut(s) 85, 86, 212
Bme18I GGWCC 2 cut(s) 88, 446
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 3 cut(s) 88, 194, 446
BmiI GGNNCC 5 cut(s) 89, 90, 127, 196, 448
BmrFI CCNGG 3 cut(s) 85, 86, 212
BoxI GACNNNNGTC 2 cut(s) 113, 327
BpmI CTGGAG 1 cut(s) 478
BpuMI CCSGG 3 cut(s) 85, 86, 212
BsaJI CCNNGG 3 cut(s) 69, 84, 210
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 375
Bse118I RCCGGY 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 69, 84, 210
BseLI CCNNNNNNNGG 2 cut(s) 349, 375
BsePI GCGCGC 1 cut(s) 47
BseXI GCAGC 3 cut(s) 152, 164, 404
BsgI GTGCAG 1 cut(s) 501
Bsh1236I CGCG 3 cut(s) 11, 49, 304
BshFI GGCC 4 cut(s) 55, 68, 195, 407
BsiHKAI GWGCWC 1 cut(s) 494
BsiHKCI CYCGRG 1 cut(s) 84
BsiSI CCGG 3 cut(s) 52, 85, 212
BslFI GGGAC 3 cut(s) 74, 432, 437
BslI CCNNNNNNNGG 2 cut(s) 349, 375
BsmFI GGGAC 3 cut(s) 74, 432, 437
BsnI GGCC 4 cut(s) 55, 68, 195, 407
BsoBI CYCGRG 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 494
BspACI CCGC 3 cut(s) 11, 104, 168
BspANI GGCC 4 cut(s) 55, 68, 195, 407
BspFNI CGCG 3 cut(s) 11, 49, 304
BspLI GGNNCC 5 cut(s) 89, 90, 127, 196, 448
BspMAI CTGCAG 2 cut(s) 413, 422
BsrFI RCCGGY 1 cut(s) 51
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 69, 84, 210
BssHII GCGCGC 1 cut(s) 47
BssT1I CCWWGG 1 cut(s) 69
Bst4CI ACNGT 2 cut(s) 26, 446
Bst6I CTCTTC 1 cut(s) 210
BstAPI GCANNNNNTGC 1 cut(s) 417
BstC8I GCNNGC 5 cut(s) 49, 53, 175, 302, 409
BstFNI CGCG 3 cut(s) 11, 49, 304
BstHHI GCGC 2 cut(s) 49, 51
BstMWI GCNNNNNNNGC 8 cut(s) 39, 48, 57, 148, 162, 174, 417, 498
BstPAI GACNNNNGTC 2 cut(s) 113, 327
BstSCI CCNGG 3 cut(s) 83, 84, 210
BstSFI CTRYAG 2 cut(s) 409, 418
BstUI CGCG 3 cut(s) 11, 49, 304
BstV1I GCAGC 3 cut(s) 152, 164, 404
BstXI CCANNNNNNTGG 1 cut(s) 463
BsuRI GGCC 4 cut(s) 55, 68, 195, 407
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 2 cut(s) 22, 158
Cac8I GCNNGC 5 cut(s) 49, 53, 175, 302, 409
CaiI CAGNNNCTG 1 cut(s) 417
CfoI GCGC 2 cut(s) 49, 51
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 3 cut(s) 88, 194, 446
Cfr9I CCCGGG 1 cut(s) 84
CviAII CATG 2 cut(s) 43, 259
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 69
Eco147I AGGCCT 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 494
Eco47I GGWCC 2 cut(s) 88, 446
Eco53kI GAGCTC 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 492
EcoO109I RGGNCCY 2 cut(s) 88, 194
EcoT14I CCWWGG 1 cut(s) 69
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 2 cut(s) 46, 262
FaiI YATR 5 cut(s) 35, 44, 260, 334, 375
FaqI GGGAC 3 cut(s) 74, 432, 437
FatI CATG 2 cut(s) 42, 258
Fnu4HI GCNGC 4 cut(s) 166, 178, 418, 499
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 4 cut(s) 166, 178, 418, 499
FspBI CTAG 1 cut(s) 507
GlaI GCGC 2 cut(s) 48, 50
GluI GCNGC 4 cut(s) 166, 178, 418, 499
GsuI CTGGAG 1 cut(s) 478
HaeIII GGCC 4 cut(s) 55, 68, 195, 407
HapII CCGG 3 cut(s) 52, 85, 212
HhaI GCGC 2 cut(s) 49, 51
Hin1II CATG 2 cut(s) 46, 262
Hin6I GCGC 2 cut(s) 47, 49
HinP1I GCGC 2 cut(s) 47, 49
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 1 cut(s) 94
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 3 cut(s) 52, 85, 212
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 2 cut(s) 442, 469
Hpy188I TCNGA 4 cut(s) 99, 125, 238, 266
Hpy188III TCNNGA 2 cut(s) 91, 475
Hpy8I GTNNAC 2 cut(s) 442, 469
Hpy99I CGWCG 2 cut(s) 112, 326
HpyAV CCTTC 3 cut(s) 58, 130, 243
HpyCH4III ACNGT 2 cut(s) 26, 446
HpyCH4IV ACGT 1 cut(s) 328
HpyCH4V TGCA 5 cut(s) 22, 180, 411, 420, 482
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 48, 57, 148, 162, 174, 417, 498
HpySE526I ACGT 1 cut(s) 328
Hsp92II CATG 2 cut(s) 46, 262
HspAI GCGC 2 cut(s) 47, 49
KflI GGGWCCC 1 cut(s) 88
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
KspAI GTTAAC 1 cut(s) 469
LmnI GCTCC 5 cut(s) 125, 139, 170, 414, 497
LpnPI CCDG 8 cut(s) 65, 74, 98, 225, 397, 406, 421, 508
Lsp1109I GCAGC 3 cut(s) 152, 164, 404
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 328
MboII GAAGA 2 cut(s) 227, 281
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 3 cut(s) 286, 392, 429
MmeI TCCRAC 3 cut(s) 122, 244, 261
MnlI CCTC 5 cut(s) 154, 208, 343, 349, 397
MroNI GCCGGC 1 cut(s) 51
MroXI GAANNNNTTC 2 cut(s) 310, 392
MseI TTAA 3 cut(s) 432, 468, 511
MspI CCGG 3 cut(s) 52, 85, 212
MspR9I CCNGG 3 cut(s) 85, 86, 212
MvnI CGCG 3 cut(s) 11, 49, 304
MwoI GCNNNNNNNGC 8 cut(s) 39, 48, 57, 148, 162, 174, 417, 498
NaeI GCCGGC 1 cut(s) 53
NciI CCSGG 3 cut(s) 85, 86, 212
NgoMIV GCCGGC 1 cut(s) 51
NlaIII CATG 2 cut(s) 46, 262
NlaIV GGNNCC 5 cut(s) 89, 90, 127, 196, 448
PalAI GGCGCGCC 1 cut(s) 47
PauI GCGCGC 1 cut(s) 47
PceI AGGCCT 1 cut(s) 407
PdiI GCCGGC 1 cut(s) 53
PdmI GAANNNNTTC 2 cut(s) 310, 392
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 4 cut(s) 167, 179, 419, 500
PpuMI RGGWCCY 1 cut(s) 88
PshAI GACNNNNGTC 2 cut(s) 113, 327
Psp124BI GAGCTC 1 cut(s) 494
Psp5II RGGWCCY 1 cut(s) 88
PspN4I GGNNCC 5 cut(s) 89, 90, 127, 196, 448
PspPI GGNCC 3 cut(s) 88, 194, 446
PspPPI RGGWCCY 1 cut(s) 88
PstI CTGCAG 2 cut(s) 413, 422
PstNI CAGNNNCTG 1 cut(s) 417
PteI GCGCGC 1 cut(s) 47
SacI GAGCTC 1 cut(s) 494
SaqAI TTAA 3 cut(s) 432, 468, 511
SatI GCNGC 4 cut(s) 166, 178, 418, 499
Sau96I GGNCC 3 cut(s) 88, 194, 446
SbfI CCTGCAGG 1 cut(s) 413
ScrFI CCNGG 3 cut(s) 85, 86, 212
SdaI CCTGCAGG 1 cut(s) 413
SduI GDGCHC 1 cut(s) 494
SetI ASST 6 cut(s) 175, 249, 331, 360, 419, 494
SfcI CTRYAG 2 cut(s) 409, 418
SgsI GGCGCGCC 1 cut(s) 47
SinI GGWCC 2 cut(s) 88, 446
SmaI CCCGGG 1 cut(s) 86
SpeI ACTAGT 1 cut(s) 506
Sse8387I CCTGCAGG 1 cut(s) 413
Sse9I AATT 3 cut(s) 286, 392, 429
SseBI AGGCCT 1 cut(s) 407
SsiI CCGC 3 cut(s) 11, 104, 168
SspMI CTAG 1 cut(s) 507
SstI GAGCTC 1 cut(s) 494
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 3 cut(s) 83, 84, 210
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 26, 446
TaiI ACGT 1 cut(s) 331
TaqI TCGA 4 cut(s) 5, 186, 243, 314
TasI AATT 3 cut(s) 286, 392, 429
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 3 cut(s) 432, 468, 511
Tru9I TTAA 3 cut(s) 432, 468, 511
TscAI CASTG 2 cut(s) 29, 158
TseI GCWGC 4 cut(s) 165, 177, 417, 498
TspMI CCCGGG 1 cut(s) 84
TspRI CASTG 2 cut(s) 29, 158
VpaK11BI GGWCC 2 cut(s) 88, 446
XapI RAATTY 2 cut(s) 286, 392
XmaI CCCGGG 1 cut(s) 84
XmnI GAANNNNTTC 2 cut(s) 310, 392
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.