pycom12g18290

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0006981
Physical Location & Seq
Forward (+)
20473637 .. 20474715
1079 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 594 bp
ATGGTTGGAATGCTTGCCGACCCAAAGGAGGCTGACGGGTTCGCTGGAGTGTTTCCAGAGCACAAATACGAAATTGTGAAGAAGTTGCAGGAAAGAAAGCACATTTGTGGAATGACTGGAGATGGTGTCAATGATGCCCCTGCACTGAAGAAGGCAGATATCGGAATTGCTGTTGCTGATGCTACAGATGCTGCACGAAGTGCTTCCGACATTGTTCTTACAGAACCCGGATTGAGTGTCATCATTAGTGCAGTGTTGACCAGTAGAGCTATTTTCCAGAGAATGAAGAATTACACTATCTATGCAGTTTCTATCACCATTCGTATTGTGTTCGGTTTCATGTTCATTGCTCTCATATGGAAGTTCGACTTCTCACCCTTCATGGTTTTGATTATTGCCATATTGAACGATGGCACAATTATGACAATCTCAAAGGATAGAGTAAAGCCATCTCCCCTGCCTGATAGCTGGAAATTGAAAGAGATTTTTGCTACTGGGATTGTGCTTGGAGGTTACTTGGCACTGATGACTGTTATCTTCTTCTGGTTAATCAAAGAAACCGACTTCTTCTCTGACTCATTAACCTTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

198

Amino Acids

21.7

Weight (kDa)

6.11

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 11 - 53 3.8e-06 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 200
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 2 cut(s) 406, 478
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 2 cut(s) 269, 468
AluI AGCT 2 cut(s) 269, 468
Alw21I GWGCWC 1 cut(s) 63
AlwNI CAGNNNCTG 1 cut(s) 191
ApeKI GCWGC 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 202
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 307, 366
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 178
BccI CCATC 3 cut(s) 116, 404, 457
BcnI CCSGG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 183
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
Bme1390I CCNGG 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 504
BmsI GCATC 3 cut(s) 124, 169, 178
BmuI ACTGGG 1 cut(s) 504
BpmI CTGGAG 2 cut(s) 66, 138
BpuMI CCSGG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 585
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse1I ACTGG 3 cut(s) 121, 261, 499
Bse3DI GCAATG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 585
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMI GCAATG 1 cut(s) 345
BseNI ACTGG 3 cut(s) 121, 261, 499
BseXI GCAGC 1 cut(s) 178
BsgI GTGCAG 3 cut(s) 126, 177, 270
BsiHKAI GWGCWC 1 cut(s) 63
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 28
BsmI GAATGC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 63
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 3 cut(s) 121, 261, 499
BssECI CCNNGG 1 cut(s) 585
BssT1I CCWWGG 1 cut(s) 585
Bst4CI ACNGT 1 cut(s) 532
BstAPI GCANNNNNTGC 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 188, 200
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 3 cut(s) 143, 258, 521
Cac8I GCNNGC 1 cut(s) 15
CaiI CAGNNNCTG 1 cut(s) 191
CviAII CATG 2 cut(s) 340, 382
CviJI RGCY 4 cut(s) 32, 269, 448, 468
CviKI_1 RGCY 4 cut(s) 32, 269, 448, 468
DraIII CACNNNGTG 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 585
Eco32I GATATC 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 167
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 585
FaeI CATG 2 cut(s) 343, 385
FaiI YATR 7 cut(s) 303, 341, 356, 358, 383, 401, 422
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FatI CATG 2 cut(s) 339, 381
FauNDI CATATG 1 cut(s) 356
Fnu4HI GCNGC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 192
GluI GCNGC 1 cut(s) 192
GsuI CTGGAG 2 cut(s) 66, 138
HapII CCGG 1 cut(s) 228
Hin1II CATG 2 cut(s) 343, 385
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 1 cut(s) 575
HpaII CCGG 1 cut(s) 228
HphI GGTGA 2 cut(s) 307, 366
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 3 cut(s) 164, 208, 574
Hpy188III TCNNGA 2 cut(s) 56, 277
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 2 cut(s) 145, 388
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4V TGCA 5 cut(s) 88, 143, 194, 251, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 200
Hsp92II CATG 2 cut(s) 343, 385
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 3 cut(s) 124, 169, 178
MaeIII GTNAC 1 cut(s) 512
MboII GAAGA 6 cut(s) 91, 160, 298, 529, 532, 559
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 5 cut(s) 72, 165, 289, 417, 473
MlyI GAGTC 1 cut(s) 569
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 2 cut(s) 22, 503
MroXI GAANNNNTTC 1 cut(s) 202
MseI TTAA 2 cut(s) 548, 581
MslI CAYNNNNRTG 2 cut(s) 105, 419
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 188, 200
NciI CCSGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 356
NlaIII CATG 2 cut(s) 343, 385
OliI CACNNNNGTG 1 cut(s) 105
PctI GAATGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 1 cut(s) 569
PpsI GAGTC 1 cut(s) 569
PstNI CAGNNNCTG 1 cut(s) 191
RseI CAYNNNNRTG 2 cut(s) 105, 419
SaqAI TTAA 2 cut(s) 548, 581
SatI GCNGC 1 cut(s) 192
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 63
SetI ASST 4 cut(s) 271, 470, 514, 587
SfaNI GCATC 3 cut(s) 124, 169, 178
SfcI CTRYAG 1 cut(s) 183
SmiMI CAYNNNNRTG 2 cut(s) 105, 419
Sse9I AATT 5 cut(s) 72, 165, 289, 417, 473
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 1 cut(s) 532
TaqI TCGA 1 cut(s) 366
TasI AATT 5 cut(s) 72, 165, 289, 417, 473
Tru1I TTAA 2 cut(s) 548, 581
Tru9I TTAA 2 cut(s) 548, 581
TscAI CASTG 3 cut(s) 150, 258, 528
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 4 cut(s) 299, 328, 334, 370
TspRI CASTG 3 cut(s) 150, 258, 528
XmnI GAANNNNTTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.