pycom12g20560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0018452
Physical Location & Seq
Forward (+)
22031276 .. 22031804
529 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 486 bp
ATGTCTGTTACAAGTAGAAGTAAATTGACGTGTGTGAATATAATCTTTCGTTACGTATTCCCTTTACAACTCCTCCCTTTAAAAACTTGCCAACTTTCTCTACACGCCCTATCTCTCTCCTCTCTATCCTCTCTCCCCTTTCTCTCTCTCTCCTCTCTCTCTCAACCAAAAGAACCCCAAAAGTTGGATTTTATACTCTCCACATGTTTGCTTAAAGAGAAGCTGAAGGGAGACAAAGACATCATTTTTGGATCGACAGAAGCCATGTTCATCACAAGGAATCAAGTCATTGGCCTTCTTTTGATCTTCTTGGGCATCTGCAGCTTACACGACAGCAGCGGCGTTTCCGGAGCTAGATGCTTAAACGAGAAGGTGGAAAATCTGGAGAAGCCTCAAGTTTTCAACTCCCCGGCTGATGATGAAGGTTTTTTTGCCACAATCAACAGACAAGTGCCTTCTTCTCCTGACCCATTGCACAATAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

17.8

Weight (kDa)

8.31

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018452)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05720
malus_domestica MD04G1206200.v1.1
prunus_persica Prupe.6G326900_v2.0.a1
pyrus_communis pycom04g18240 pycom12g20560
rosa_chinensis RchiOBHm_Chr3g0454611
rosa_roxburghii Rroxscaffold_6G00424930
rosa_rugosa Rorug03G0000600
rosa_samantha Rh3AG060500 Rh3DG062200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 184
AccIII TCCGGA 1 cut(s) 347
AciI CCGC 1 cut(s) 339
AclWI GGATC 1 cut(s) 259
AcuI CTGAAG 1 cut(s) 245
AfiI CCNNNNNNNGG 1 cut(s) 184
AflIII ACRYGT 2 cut(s) 29, 203
AgsI TTSAA 1 cut(s) 403
AjiI CACGTC 1 cut(s) 30
AluBI AGCT 3 cut(s) 223, 324, 353
AluI AGCT 3 cut(s) 223, 324, 353
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 1 cut(s) 259
Aor13HI TCCGGA 1 cut(s) 347
AoxI GGCC 1 cut(s) 292
ApeKI GCWGC 2 cut(s) 321, 336
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AsuC2I CCSGG 1 cut(s) 410
BbvI GCAGC 2 cut(s) 333, 348
BcnI CCSGG 1 cut(s) 410
BcoDI GTCTC 1 cut(s) 225
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 319
BisI GCNGC 3 cut(s) 322, 337, 340
BlsI GCNGC 3 cut(s) 323, 338, 341
Bme1390I CCNGG 1 cut(s) 410
BmgBI CACGTC 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 410
BmsI GCATC 2 cut(s) 324, 347
BpmI CTGGAG 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 410
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 408
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 184
Bse3DI GCAATG 1 cut(s) 470
BseAI TCCGGA 1 cut(s) 347
BseDI CCNNGG 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 184
BseMI GCAATG 1 cut(s) 470
BseRI GAGGAG 3 cut(s) 62, 109, 142
BseXI GCAGC 2 cut(s) 333, 348
BshFI GGCC 1 cut(s) 294
BsiSI CCGG 2 cut(s) 348, 410
BslI CCNNNNNNNGG 1 cut(s) 184
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 1 cut(s) 294
Bsp13I TCCGGA 1 cut(s) 347
Bsp143I GATC 2 cut(s) 251, 303
BspACI CCGC 1 cut(s) 339
BspANI GGCC 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 347
BspMAI CTGCAG 1 cut(s) 323
BspPI GGATC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 470
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 2 cut(s) 251, 303
BstBAI YACGTR 1 cut(s) 55
BstKTI GATC 2 cut(s) 254, 306
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 2 cut(s) 251, 303
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 408
BstSFI CTRYAG 1 cut(s) 319
BstSNI TACGTA 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 333, 348
BsuRI GGCC 1 cut(s) 294
BtrI CACGTC 1 cut(s) 30
CviAII CATG 2 cut(s) 204, 265
CviJI RGCY 7 cut(s) 223, 263, 294, 324, 353, 391, 413
CviKI_1 RGCY 7 cut(s) 223, 263, 294, 324, 353, 391, 413
DpnI GATC 2 cut(s) 253, 305
DpnII GATC 2 cut(s) 251, 303
DraI TTTAAA 1 cut(s) 81
Eco105I TACGTA 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 245
FaeI CATG 2 cut(s) 207, 268
FaiI YATR 4 cut(s) 41, 194, 205, 266
FatI CATG 2 cut(s) 203, 264
Fnu4HI GCNGC 3 cut(s) 322, 337, 340
Fsp4HI GCNGC 3 cut(s) 322, 337, 340
FspBI CTAG 1 cut(s) 354
GluI GCNGC 3 cut(s) 322, 337, 340
GsuI CTGGAG 1 cut(s) 404
HaeIII GGCC 1 cut(s) 294
HapII CCGG 2 cut(s) 348, 410
Hin1II CATG 2 cut(s) 207, 268
HinfI GANTC 1 cut(s) 280
HpaII CCGG 2 cut(s) 348, 410
Hpy188III TCNNGA 3 cut(s) 348, 383, 464
HpyAV CCTTC 5 cut(s) 220, 305, 364, 416, 465
HpyCH4IV ACGT 2 cut(s) 29, 54
HpyCH4V TGCA 2 cut(s) 321, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpySE526I ACGT 2 cut(s) 29, 54
Hsp92II CATG 2 cut(s) 207, 268
Kpn2I TCCGGA 1 cut(s) 347
Kzo9I GATC 2 cut(s) 251, 303
LmnI GCTCC 1 cut(s) 350
LpnPI CCDG 4 cut(s) 361, 368, 423, 477
Lsp1109I GCAGC 2 cut(s) 333, 348
LweI GCATC 2 cut(s) 324, 347
MaeI CTAG 1 cut(s) 354
MaeII ACGT 2 cut(s) 29, 54
MaeIII GTNAC 2 cut(s) 7, 50
MalI GATC 2 cut(s) 253, 305
MboI GATC 2 cut(s) 251, 303
MboII GAAGA 2 cut(s) 298, 450
MluCI AATT 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 5 cut(s) 83, 130, 139, 163, 402
MroI TCCGGA 1 cut(s) 347
MseI TTAA 3 cut(s) 80, 213, 362
MspA1I CMGCKG 1 cut(s) 339
MspI CCGG 2 cut(s) 348, 410
MspR9I CCNGG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 321
NciI CCSGG 1 cut(s) 410
NdeII GATC 2 cut(s) 251, 303
NlaIII CATG 2 cut(s) 207, 268
NspI RCATGY 1 cut(s) 207
PciI ACATGT 1 cut(s) 203
PfeI GAWTC 1 cut(s) 280
PflMI CCANNNNNTGG 1 cut(s) 184
PkrI GCNGC 3 cut(s) 323, 338, 341
Ppu21I YACGTR 1 cut(s) 55
PscI ACATGT 1 cut(s) 203
PstI CTGCAG 1 cut(s) 323
SaqAI TTAA 3 cut(s) 80, 213, 362
SatI GCNGC 3 cut(s) 322, 337, 340
Sau3AI GATC 2 cut(s) 251, 303
ScrFI CCNGG 1 cut(s) 410
SetI ASST 8 cut(s) 32, 57, 225, 326, 355, 375, 427, 485
SfaNI GCATC 2 cut(s) 324, 347
SfcI CTRYAG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
SnaBI TACGTA 1 cut(s) 55
Sse9I AATT 1 cut(s) 23
SsiI CCGC 1 cut(s) 339
SspMI CTAG 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 408
TaiI ACGT 2 cut(s) 32, 57
TaqI TCGA 1 cut(s) 254
TasI AATT 1 cut(s) 23
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 3 cut(s) 80, 213, 362
Tru9I TTAA 3 cut(s) 80, 213, 362
TseI GCWGC 2 cut(s) 321, 336
TspDTI ATGAA 2 cut(s) 259, 435
Van91I CCANNNNNTGG 1 cut(s) 184
XceI RCATGY 1 cut(s) 207
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.