Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0011297

Physical Location & Seq

Genomic Coordinates Forward (+)

22780065 .. 22783086

Gene Structure 3022 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1851 bp

ATGGAATCATCTAACGGTCCAGATTCCACCGATGGGTTGCCATCGATTGACTTTTCGGATTCTCCTCCTTCTCTTCCCACCATCTGTACGGACGTCAATGTCGTCCCTGAGCACGAAAGGAATGAGCTTCAGCAGTCGATATCAAATCTTGAAGGAGAGATTGCACAGTTGAAGCAGAAGCAGAAGTTCTTGGATGAGAAGAGGAGAGAATCTTTGAACAAGATTATAGACATCAAAGGTAGTATTCGAGTGTTCTGTCGGGTCCGACCGTTCCTAGTGATCCACAGGAGAAGAATCCGGGAACCCATTTCGGCCGCATCGGAGAAGATTGCGGTTAAGACAGCTGGAACGAGAAAAGAATTTGATTTTGATAAGGTTTTTCCACAAGAAACCAGTCAAGAAAATGTGTTTGCTGAGGTGGAACCAATTCTCAGATCTGCACTTGATGGCCACAATGTGTGTATTTTTGCTCACGGCCAAACTGGCACGGGCAAAACATATACCATGGATGGAACTAGTGAGGAGCCCGGGATAATTCCTCGAGCTCTCAAGGAGCTTTTCTGTCAAGCCTCCTTGGATAGCTCGTCCTCTATTTCCTTTTCAATGAGCATGTTGGAAGTTTACATGGGCAATCTCAGAGATCTACTGTCCCCAAAACCCGCCAACAGAACATACGAAACTGTAACAAGATGCAATCTCAACATTCAAACAGATCCGAAGGGAATGGTGGAAATCGAGGGTCTTACAGAAGTGCAAATTGTCGATTTTGCTAAAGCTAGGTGGTGGTATAATCGGGGGAGGCGAGTTAGATCAACTTCTTGGACGAATGTGAATGAGGCGTCTAGCAGGTCTCACTGCTTAACGAGGATAACCATATTTCGGAATGGGGATGCCCTGGAAGCTAAAAGGGAAGTGGGCAAACTGTGGATGGTTGATCTTGGAGGAAGCGAACGGTTGCTTAAAACGGGGGCAACTGGACTTACACTTGATGAGGGAAGGGCCATAAACCTTTCTCTTTCCGCTTTGGGTGATGTCATTGCTGCTCTACGAAGGAAGCGAGGCCATGTGCCTTACAGGAACAGCAAGCTGACACAAATCCTGAAAGACTCCTTAGGTCACGGTTCAAAGGTTTTGATGCTTGTACATGTCAGCCCCTGTGAAGAAGATGTTGGGGAGACGATTTGCTCTTTGAGTTTCGCCAAGAGAGCTAGAGCTATAGAGTGTAACAGGGAATTATCAGAGGACTTGAAGATGCACAGGGAAAAAAGGGTGAAGGAACTCGAAGAAGACATGAGAGAAGCTGAAGAAGAGTGCCATAAAGTTAAAAATCAGATACAAAAGGTCGATTTGCTGCTACGCAAAAATATTAACCTCTTTGCAGCCACGTATGGACATTTGAAAGATGAGAAGGCCCCCATTAGTCCCAAAGAAGTTTCGAGGGAAGTTATAGAAACGCCACGAAAGCCAGAGAAAGGAAGCAGAGGGAACAACATTTCAAACTCTTTGCCTCGGTTCATGACTTCCACTGCAGCCAGTCGACAAAGGCAAAGCTCTGCAGAAAGAGATACAGTCGGTAAGGCAAGGAGTTTGAGATCCATGGCCAGAAGTTCAATCCAATTTACAGCTTCACAGTCAATGAGTTACTCAGACCCTCAAATCAAAGCAATTTTACAAAACTCGAGTAGGAAATCACGATATGCGGAGGAAGCAGCAGATACTTTACCGGCAGAGAGCCCGAAGTGCAATGGCTCGGAATCATCCAAGACGCGAAGCAAGTTGGTCACTTCGTCCGATCCAAACTTGAGAACTATGCTTGGTCGTCATAGAAGAAGGATGTCTGATCTAATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003008 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005813 GO:0005815 GO:0005856 GO:0005871 GO:0005875 GO:0005911 GO:0005912 GO:0005913 GO:0005915 GO:0006928 GO:0007017 GO:0007018 GO:0007155 GO:0007600 GO:0007601 GO:0008150 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022610 GO:0030054 GO:0032501 GO:0032991 GO:0034330 GO:0034331 GO:0034332 GO:0034334 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043296 GO:0043954 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045216 GO:0045217 GO:0045218 GO:0050877 GO:0050953 GO:0070161 GO:0071840 GO:0090136 GO:0098609

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

617

Amino Acids

69.11

Weight (kDa)

9.22

Isoelectric Point (pI)

61.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	66 - 216	1.9e-36	Microtubule binding
Kinesin	PF00225	105 - 406	1.5e-99	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011297)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27950 AT5G27950
fragaria_vesca	FvH4_6g03950 FvH4_6g03950 FvH4_6g03950
malus_domestica	MD12G1234000.v1.1
prunus_persica	Prupe.6G337000_v2.0.a1 Prupe.6G337000_v2.0.a1
pyrus_communis	pycom12g21640
rosa_chinensis	RchiOBHm_Chr3g0452501
rosa_laevigata	RLG00000025597
rosa_multiflora	Rmu_co8493577.1_g000001 Rmu_sc0011680.1_g000003
rosa_roxburghii	Rroxscaffold_6G00429530
rosa_rugosa	Rorug02G0641200
rosa_samantha	Rh3AG043100 Rh3BG044800 Rh3CG043400 Rh3DG044200
rosa_wichuraiana	Rw3G003340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	98
AatII	GACGTC	1 cut(s)	96
Acc36I	ACCTGC	1 cut(s)	837
AccI	GTMKAC	1 cut(s)	1537
AccII	CGCG	1 cut(s)	1768
AciI	CCGC	5 cut(s)	315, 332, 660, 1020, 1700
AclWI	GGATC	4 cut(s)	274, 707, 1587, 1787
AcoI	YGGCCR	4 cut(s)	312, 448, 475, 1599
AcsI	RAATTY	1 cut(s)	359
AcuI	CTGAAG	2 cut(s)	113, 1323
AcyI	GRCGYC	2 cut(s)	93, 839
AdeI	CACNNNGTG	1 cut(s)	457
AfaI	GTAC	2 cut(s)	88, 1143
AfiI	CCNNNNNNNGG	3 cut(s)	33, 879, 1472
AflIII	ACRYGT	1 cut(s)	1144
AhlI	ACTAGT	1 cut(s)	515
AjnI	CCWGG	1 cut(s)	894
AjuI	GAANNNNNNNTTGG	2 cut(s)	1152, 1184
Alw21I	GWGCWC	2 cut(s)	114, 547
Alw26I	GTCTC	2 cut(s)	855, 1169
AlwI	GGATC	4 cut(s)	274, 707, 1587, 1787
AlwNI	CAGNNNCTG	1 cut(s)	1155
Ama87I	CYCGRG	3 cut(s)	527, 540, 1678
AoxI	GGCC	7 cut(s)	312, 448, 475, 999, 1060, 1410, 1599
ApeKI	GCWGC	5 cut(s)	1040, 1351, 1379, 1529, 1709
ApoI	RAATTY	1 cut(s)	359
Asp700I	GAANNNNTTC	1 cut(s)	426
AspS9I	GGNCC	4 cut(s)	17, 262, 999, 1411
AsuC2I	CCSGG	3 cut(s)	299, 528, 529
AsuHPI	GGTGA	2 cut(s)	1040, 1282
AvaI	CYCGRG	3 cut(s)	527, 540, 1678
AvaII	GGWCC	2 cut(s)	17, 262
AxyI	CCTNAGG	1 cut(s)	1111
BalI	TGGCCA	2 cut(s)	450, 1601
BanII	GRGCYC	3 cut(s)	528, 547, 1736
BbsI	GAAGAC	1 cut(s)	1293
Bbv12I	GWGCWC	2 cut(s)	114, 547
BbvCI	CCTCAGC	1 cut(s)	414
BbvI	GCAGC	5 cut(s)	1027, 1338, 1391, 1541, 1721
BccI	CCATC	6 cut(s)	26, 49, 89, 440, 503, 922
BceAI	ACGGC	1 cut(s)	490
BciT130I	CCWGG	1 cut(s)	896
BcnI	CCSGG	3 cut(s)	299, 528, 529
BcoDI	GTCTC	2 cut(s)	855, 1169
BcuI	ACTAGT	1 cut(s)	515
BfaI	CTAG	5 cut(s)	275, 516, 777, 843, 1209
BfmI	CTRYAG	3 cut(s)	1215, 1527, 1554
BfuAI	ACCTGC	1 cut(s)	837
BglI	GCCNNNNNGGC	1 cut(s)	483
BglII	AGATCT	2 cut(s)	434, 640
BisI	GCNGC	6 cut(s)	315, 1041, 1352, 1380, 1530, 1710
BlsI	GCNGC	6 cut(s)	316, 1042, 1353, 1381, 1531, 1711
Bme1390I	CCNGG	4 cut(s)	299, 528, 529, 896
Bme18I	GGWCC	2 cut(s)	17, 262
BmeT110I	CYCGRG	3 cut(s)	527, 540, 1678
BmgT120I	GGNCC	4 cut(s)	17, 262, 999, 1411
BmiI	GGNNCC	5 cut(s)	263, 303, 423, 525, 1413
BmrFI	CCNGG	4 cut(s)	299, 528, 529, 896
BmsI	GCATC	5 cut(s)	326, 680, 880, 1125, 1242
BpiI	GAAGAC	1 cut(s)	1293
Bpu10I	CCTNAGC	2 cut(s)	108, 414
BpuEI	CTTGAG	2 cut(s)	533, 1822
BpuMI	CCSGG	3 cut(s)	299, 528, 529
Bsa29I	ATCGAT	1 cut(s)	44
BsaAI	YACGTR	1 cut(s)	1386
BsaHI	GRCGYC	2 cut(s)	93, 839
BsaI	GGTCTC	1 cut(s)	855
BsaJI	CCNNGG	6 cut(s)	504, 527, 573, 894, 1508, 1596
Bsc4I	CCNNNNNNNGG	3 cut(s)	33, 879, 1472
Bse118I	RCCGGY	1 cut(s)	1723
Bse1I	ACTGG	4 cut(s)	393, 487, 979, 1533
Bse21I	CCTNAGG	1 cut(s)	1111
Bse3DI	GCAATG	2 cut(s)	1035, 1750
BseBI	CCWGG	1 cut(s)	896
BseCI	ATCGAT	1 cut(s)	44
BseDI	CCNNGG	6 cut(s)	504, 527, 573, 894, 1508, 1596
BseGI	GGATG	6 cut(s)	199, 514, 895, 933, 1757, 1839
BseLI	CCNNNNNNNGG	3 cut(s)	33, 879, 1472
BseMI	GCAATG	2 cut(s)	1035, 1750
BseMII	CTCAG	5 cut(s)	99, 405, 445, 649, 1659
BseNI	ACTGG	4 cut(s)	393, 487, 979, 1533
BseRI	GAGGAG	3 cut(s)	54, 217, 536
BseX3I	CGGCCG	1 cut(s)	312
BseXI	GCAGC	5 cut(s)	1027, 1338, 1391, 1541, 1721
BsgI	GTGCAG	1 cut(s)	423
Bsh1236I	CGCG	1 cut(s)	1768
Bsh1285I	CGRYCG	2 cut(s)	269, 315
BshFI	GGCC	7 cut(s)	314, 450, 477, 1001, 1062, 1412, 1601
BshVI	ATCGAT	1 cut(s)	44
BsiEI	CGRYCG	2 cut(s)	269, 315
BsiHKAI	GWGCWC	2 cut(s)	114, 547
BsiHKCI	CYCGRG	3 cut(s)	527, 540, 1678
BsiSI	CCGG	3 cut(s)	298, 528, 1724
BslFI	GGGAC	3 cut(s)	89, 634, 1407
BslI	CCNNNNNNNGG	3 cut(s)	33, 879, 1472
BsmAI	GTCTC	2 cut(s)	855, 1169
BsmBI	CGTCTC	1 cut(s)	1169
BsmFI	GGGAC	3 cut(s)	89, 634, 1407
BsnI	GGCC	7 cut(s)	314, 450, 477, 1001, 1062, 1412, 1601
Bso31I	GGTCTC	1 cut(s)	855
BsoBI	CYCGRG	3 cut(s)	527, 540, 1678
Bsp1286I	GDGCHC	4 cut(s)	114, 528, 547, 1736
Bsp1407I	TGTACA	1 cut(s)	1141
Bsp143I	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
Bsp19I	CCATGG	2 cut(s)	504, 1596
BspACI	CCGC	5 cut(s)	315, 332, 660, 1020, 1700
BspANI	GGCC	7 cut(s)	314, 450, 477, 1001, 1062, 1412, 1601
BspCNI	CTCAG	5 cut(s)	100, 406, 444, 648, 1658
BspDI	ATCGAT	1 cut(s)	44
BspFNI	CGCG	1 cut(s)	1768
BspHI	TCATGA	1 cut(s)	1515
BspLI	GGNNCC	5 cut(s)	263, 303, 423, 525, 1413
BspMAI	CTGCAG	2 cut(s)	1531, 1558
BspMI	ACCTGC	1 cut(s)	837
BspPI	GGATC	4 cut(s)	274, 707, 1587, 1787
BspTNI	GGTCTC	1 cut(s)	855
BsrDI	GCAATG	2 cut(s)	1035, 1750
BsrFI	RCCGGY	1 cut(s)	1723
BsrGI	TGTACA	1 cut(s)	1141
BsrI	ACTGG	4 cut(s)	393, 487, 979, 1533
BssAI	RCCGGY	1 cut(s)	1723
BssECI	CCNNGG	6 cut(s)	504, 527, 573, 894, 1508, 1596
BssMI	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
BssNI	GRCGYC	2 cut(s)	93, 839
BssT1I	CCWWGG	3 cut(s)	504, 573, 1596
Bst2UI	CCWGG	1 cut(s)	896
Bst6I	CTCTTC	3 cut(s)	78, 194, 1302
BstACI	GRCGYC	2 cut(s)	93, 839
BstAUI	TGTACA	1 cut(s)	1141
BstBAI	YACGTR	1 cut(s)	1386
BstC8I	GCNNGC	1 cut(s)	1085
BstDEI	CTNAG	6 cut(s)	108, 414, 431, 635, 1111, 1645
BstDSI	CCRYGG	2 cut(s)	504, 1596
BstF5I	GGATG	6 cut(s)	199, 514, 895, 933, 1757, 1839
BstFNI	CGCG	1 cut(s)	1768
BstKTI	GATC	9 cut(s)	282, 437, 643, 715, 812, 937, 1595, 1795, 1843
BstMAI	GTCTC	2 cut(s)	855, 1169
BstMBI	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
BstMCI	CGRYCG	2 cut(s)	269, 315
BstMWI	GCNNNNNNNGC	6 cut(s)	483, 899, 1205, 1462, 1706, 1740
BstNI	CCWGG	1 cut(s)	896
BstNSI	RCATGY	2 cut(s)	613, 1148
BstSCI	CCNGG	4 cut(s)	297, 526, 527, 894
BstSFI	CTRYAG	3 cut(s)	1215, 1527, 1554
BstUI	CGCG	1 cut(s)	1768
BstV1I	GCAGC	5 cut(s)	1027, 1338, 1391, 1541, 1721
BstV2I	GAAGAC	1 cut(s)	1293
BstX2I	RGATCY	4 cut(s)	434, 640, 712, 1592
BstYI	RGATCY	4 cut(s)	434, 640, 712, 1592
BstZI	CGGCCG	1 cut(s)	312
Bsu15I	ATCGAT	1 cut(s)	44
Bsu36I	CCTNAGG	1 cut(s)	1111
BsuRI	GGCC	7 cut(s)	314, 450, 477, 1001, 1062, 1412, 1601
BsuTUI	ATCGAT	1 cut(s)	44
BtgI	CCRYGG	2 cut(s)	504, 1596
BtsCI	GGATG	6 cut(s)	199, 514, 895, 933, 1757, 1839
BtsI	GCAGTG	2 cut(s)	853, 1524
BtsIMutI	CAGTG	2 cut(s)	853, 1524
BveI	ACCTGC	1 cut(s)	837
Cac8I	GCNNGC	1 cut(s)	1085
CaiI	CAGNNNCTG	1 cut(s)	1155
CciI	TCATGA	1 cut(s)	1515
Cfr10I	RCCGGY	1 cut(s)	1723
Cfr13I	GGNCC	4 cut(s)	17, 262, 999, 1411
Cfr9I	CCCGGG	1 cut(s)	527
ClaI	ATCGAT	1 cut(s)	44
CseI	GACGC	2 cut(s)	828, 1774
Csp6I	GTAC	2 cut(s)	87, 1142
CviAII	CATG	8 cut(s)	505, 610, 625, 1064, 1145, 1291, 1516, 1597
CviQI	GTAC	2 cut(s)	87, 1142
DdeI	CTNAG	6 cut(s)	108, 414, 431, 635, 1111, 1645
DpnI	GATC	9 cut(s)	281, 436, 642, 714, 811, 936, 1594, 1794, 1842
DpnII	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
DraIII	CACNNNGTG	1 cut(s)	457
DrdI	GACNNNNNNGTC	1 cut(s)	98
DseDI	GACNNNNNNGTC	1 cut(s)	98
EaeI	YGGCCR	4 cut(s)	312, 448, 475, 1599
EagI	CGGCCG	1 cut(s)	312
Eam1104I	CTCTTC	3 cut(s)	78, 194, 1302
EarI	CTCTTC	3 cut(s)	78, 194, 1302
Ecl136II	GAGCTC	1 cut(s)	545
EclXI	CGGCCG	1 cut(s)	312
Eco130I	CCWWGG	3 cut(s)	504, 573, 1596
Eco24I	GRGCYC	3 cut(s)	528, 547, 1736
Eco31I	GGTCTC	1 cut(s)	855
Eco32I	GATATC	1 cut(s)	141
Eco47I	GGWCC	2 cut(s)	17, 262
Eco52I	CGGCCG	1 cut(s)	312
Eco53kI	GAGCTC	1 cut(s)	545
Eco57I	CTGAAG	2 cut(s)	113, 1323
Eco81I	CCTNAGG	1 cut(s)	1111
Eco88I	CYCGRG	3 cut(s)	527, 540, 1678
EcoICRI	GAGCTC	1 cut(s)	545
EcoO109I	RGGNCCY	1 cut(s)	1411
EcoRII	CCWGG	1 cut(s)	894
EcoRV	GATATC	1 cut(s)	141
EcoT14I	CCWWGG	3 cut(s)	504, 573, 1596
EcoT38I	GRGCYC	3 cut(s)	528, 547, 1736
ErhI	CCWWGG	3 cut(s)	504, 573, 1596
Esp3I	CGTCTC	1 cut(s)	1169
FaeI	CATG	8 cut(s)	508, 613, 628, 1067, 1148, 1294, 1519, 1600
FaqI	GGGAC	3 cut(s)	89, 634, 1407
FatI	CATG	8 cut(s)	504, 609, 624, 1063, 1144, 1290, 1515, 1596
FauI	CCCGC	1 cut(s)	667
FblI	GTMKAC	1 cut(s)	1537
Fnu4HI	GCNGC	6 cut(s)	315, 1041, 1352, 1380, 1530, 1710
FokI	GGATG	6 cut(s)	206, 521, 902, 940, 1744, 1846
FriOI	GRGCYC	3 cut(s)	528, 547, 1736
Fsp4HI	GCNGC	6 cut(s)	315, 1041, 1352, 1380, 1530, 1710
FspBI	CTAG	5 cut(s)	275, 516, 777, 843, 1209
GluI	GCNGC	6 cut(s)	315, 1041, 1352, 1380, 1530, 1710
HaeIII	GGCC	7 cut(s)	314, 450, 477, 1001, 1062, 1412, 1601
HapII	CCGG	3 cut(s)	298, 528, 1724
HgaI	GACGC	2 cut(s)	828, 1774
Hin1I	GRCGYC	2 cut(s)	93, 839
Hin1II	CATG	8 cut(s)	508, 613, 628, 1067, 1148, 1294, 1519, 1600
HincII	GTYRAC	1 cut(s)	1538
HindII	GTYRAC	1 cut(s)	1538
HinfI	GANTC	7 cut(s)	5, 23, 59, 209, 294, 1106, 1754
HpaII	CCGG	3 cut(s)	298, 528, 1724
HphI	GGTGA	2 cut(s)	1040, 1282
Hpy166II	GTNNAC	2 cut(s)	622, 1538
Hpy188III	TCNNGA	6 cut(s)	20, 149, 398, 1099, 1516, 1692
Hpy8I	GTNNAC	2 cut(s)	622, 1538
HpyAV	CCTTC	8 cut(s)	78, 146, 712, 990, 1044, 1267, 1402, 1824
HpyCH4IV	ACGT	2 cut(s)	93, 1385
HpyCH4V	TGCA	9 cut(s)	164, 440, 693, 754, 1255, 1379, 1529, 1556, 1743
HpyF10VI	GCNNNNNNNGC	6 cut(s)	483, 899, 1205, 1462, 1706, 1740
HpyF3I	CTNAG	6 cut(s)	108, 414, 431, 635, 1111, 1645
HpySE526I	ACGT	2 cut(s)	93, 1385
Hsp92I	GRCGYC	2 cut(s)	93, 839
Hsp92II	CATG	8 cut(s)	508, 613, 628, 1067, 1148, 1294, 1519, 1600
Kzo9I	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
LmnI	GCTCC	2 cut(s)	523, 553
Lsp1109I	GCAGC	5 cut(s)	1027, 1338, 1391, 1541, 1721
LweI	GCATC	5 cut(s)	326, 680, 880, 1125, 1242
MaeI	CTAG	5 cut(s)	275, 516, 777, 843, 1209
MaeII	ACGT	2 cut(s)	93, 1385
MaeIII	GTNAC	5 cut(s)	682, 1115, 1223, 1640, 1780
MalI	GATC	9 cut(s)	281, 436, 642, 714, 811, 936, 1594, 1794, 1842
MboI	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
MflI	RGATCY	4 cut(s)	434, 640, 712, 1592
MhlI	GDGCHC	4 cut(s)	114, 528, 547, 1736
MlsI	TGGCCA	2 cut(s)	450, 1601
MluCI	AATT	7 cut(s)	359, 426, 534, 756, 1232, 1616, 1665
MluNI	TGGCCA	2 cut(s)	450, 1601
MlyI	GAGTC	1 cut(s)	1100
MmeI	TCCRAC	2 cut(s)	289, 594
Mox20I	TGGCCA	2 cut(s)	450, 1601
MroXI	GAANNNNTTC	1 cut(s)	426
MscI	TGGCCA	2 cut(s)	450, 1601
MseI	TTAA	5 cut(s)	336, 860, 960, 1323, 1368
Msp20I	TGGCCA	2 cut(s)	450, 1601
MspA1I	CMGCKG	1 cut(s)	344
MspI	CCGG	3 cut(s)	298, 528, 1724
MspR9I	CCNGG	4 cut(s)	299, 528, 529, 896
MvaI	CCWGG	1 cut(s)	896
MvnI	CGCG	1 cut(s)	1768
MwoI	GCNNNNNNNGC	6 cut(s)	483, 899, 1205, 1462, 1706, 1740
NciI	CCSGG	3 cut(s)	299, 528, 529
NcoI	CCATGG	2 cut(s)	504, 1596
NdeII	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
NlaIII	CATG	8 cut(s)	508, 613, 628, 1067, 1148, 1294, 1519, 1600
NlaIV	GGNNCC	5 cut(s)	263, 303, 423, 525, 1413
NmuCI	GTSAC	2 cut(s)	1115, 1780
NspI	RCATGY	2 cut(s)	613, 1148
PaeR7I	CTCGAG	2 cut(s)	540, 1678
PagI	TCATGA	1 cut(s)	1515
PciI	ACATGT	1 cut(s)	1144
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 799, 1054
PdmI	GAANNNNTTC	1 cut(s)	426
PfeI	GAWTC	6 cut(s)	5, 23, 59, 209, 294, 1754
PfoI	TCCNGGA	1 cut(s)	297
PkrI	GCNGC	6 cut(s)	316, 1042, 1353, 1381, 1531, 1711
PleI	GAGTC	1 cut(s)	1100
PpsI	GAGTC	1 cut(s)	1100
Ppu21I	YACGTR	1 cut(s)	1386
PscI	ACATGT	1 cut(s)	1144
Psp124BI	GAGCTC	1 cut(s)	547
Psp6I	CCWGG	1 cut(s)	894
PspGI	CCWGG	1 cut(s)	894
PspN4I	GGNNCC	5 cut(s)	263, 303, 423, 525, 1413
PspPI	GGNCC	4 cut(s)	17, 262, 999, 1411
PspXI	VCTCGAGB	2 cut(s)	540, 1678
PstI	CTGCAG	2 cut(s)	1531, 1558
PstNI	CAGNNNCTG	1 cut(s)	1155
PsuI	RGATCY	4 cut(s)	434, 640, 712, 1592
PvuII	CAGCTG	1 cut(s)	344
RsaI	GTAC	2 cut(s)	88, 1143
RsaNI	GTAC	2 cut(s)	87, 1142
SacI	GAGCTC	1 cut(s)	547
SalI	GTCGAC	1 cut(s)	1536
SaqAI	TTAA	5 cut(s)	336, 860, 960, 1323, 1368
SatI	GCNGC	6 cut(s)	315, 1041, 1352, 1380, 1530, 1710
Sau3AI	GATC	9 cut(s)	279, 434, 640, 712, 809, 934, 1592, 1792, 1840
Sau96I	GGNCC	4 cut(s)	17, 262, 999, 1411
SchI	GAGTC	1 cut(s)	1100
ScrFI	CCNGG	4 cut(s)	299, 528, 529, 896
SduI	GDGCHC	4 cut(s)	114, 528, 547, 1736
SfaNI	GCATC	5 cut(s)	326, 680, 880, 1125, 1242
SfcI	CTRYAG	3 cut(s)	1215, 1527, 1554
Sfr274I	CTCGAG	2 cut(s)	540, 1678
SinI	GGWCC	2 cut(s)	17, 262
SlaI	CTCGAG	2 cut(s)	540, 1678
SmaI	CCCGGG	1 cut(s)	529
SmlI	CTYRAG	4 cut(s)	540, 548, 1678, 1801
SmoI	CTYRAG	4 cut(s)	540, 548, 1678, 1801
SpeI	ACTAGT	1 cut(s)	515
Sse9I	AATT	7 cut(s)	359, 426, 534, 756, 1232, 1616, 1665
SsiI	CCGC	5 cut(s)	315, 332, 660, 1020, 1700
SspI	AATATT	1 cut(s)	1366
SspMI	CTAG	5 cut(s)	275, 516, 777, 843, 1209
SstI	GAGCTC	1 cut(s)	547
StyD4I	CCNGG	4 cut(s)	297, 526, 527, 894
StyI	CCWWGG	3 cut(s)	504, 573, 1596
TaiI	ACGT	2 cut(s)	96, 1388
TasI	AATT	7 cut(s)	359, 426, 534, 756, 1232, 1616, 1665
TatI	WGTACW	1 cut(s)	1141
TauI	GCSGC	1 cut(s)	317
TfiI	GAWTC	6 cut(s)	5, 23, 59, 209, 294, 1754
Tru1I	TTAA	5 cut(s)	336, 860, 960, 1323, 1368
Tru9I	TTAA	5 cut(s)	336, 860, 960, 1323, 1368
TscAI	CASTG	2 cut(s)	860, 1531
TseFI	GTSAC	2 cut(s)	1115, 1780
TseI	GCWGC	5 cut(s)	1040, 1351, 1379, 1529, 1709
Tsp45I	GTSAC	2 cut(s)	1115, 1780
TspDTI	ATGAA	1 cut(s)	1504
TspGWI	ACGGA	1 cut(s)	104
TspMI	CCCGGG	1 cut(s)	527
TspRI	CASTG	2 cut(s)	860, 1531
VpaK11BI	GGWCC	2 cut(s)	17, 262
XapI	RAATTY	1 cut(s)	359
XceI	RCATGY	2 cut(s)	613, 1148
XhoI	CTCGAG	2 cut(s)	540, 1678
XmaI	CCCGGG	1 cut(s)	527
XmiI	GTMKAC	1 cut(s)	1537
XmnI	GAANNNNTTC	1 cut(s)	426
XspI	CTAG	5 cut(s)	275, 516, 777, 843, 1209
ZraI	GACGTC	1 cut(s)	94

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.