pycom13g04220

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014445
Physical Location & Seq
Reverse (-)
2839093 .. 2840839
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 735 bp
ATGGCGACAGTCACACCTTCACTAGTTCCACTCCTCTTCTTTGCCAGAATCTCAGGGCTTGCAGTTGCAGCTCTGGTCATTTTCTGGGCTTTCTCCTTCAAGTCCAGCTTCCTTCCTCACTCCTCCCCACAAGAACACCAAGCCCTCATCTATTCAGCTCTTCATCCTCTGTTCATGGTCATTGGGTTTATTCTCATTAGTGGAGAAGCAATTTTGATCCATAGGTGGTTGCCTGGTTCAAGGAGTTTGAAGAAATCAGTGCATTTGTGTCTTCAAGGCATGGCTTTGGCTTCTGGGATAGTTGGGATTTGGACTAAATTTCAAGGGAAGAAGGGCATTGTGGCTAATTTCTATAGTTTGCATTCTTGGATGGGGATGATCTGCATCCTCTTGTTTGGAGCTCAGTGGTTGATGGGGTTCCTGAGCTTCTGGCATCGAGGGGAAGTGCGCTCGGTGAGGCTAAGGGTGTTGCCGTGGCACATCTTCCTTGGACTCTACACGTACGGTCTGGCCGTGGCAACTGCAGAAACGGGGCTTTTGGAGAAGTTGACATTCTTACAAACGAAGAGGAATGTGTCTAAACACTGTACCGAGTCGATGGTTGTGAATAGTTTGGGACTGGGGCTGGCATTGGTCAGCGGCATTGTAATTTTGGCTGCAGTGTCGCCTAAATACCAAGCCCTGCAAAGTAAACTCATTTACTCATCAGACAGCAAAAAGTCCATGTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

245

Amino Acids

26.89

Weight (kDa)

10.06

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 54 - 185 8.7e-41 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014445)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26100
fragaria_vesca FvH4_4g28220 FvH4_4g28220 FvH4_4g28220 FvH4_4g28220
malus_domestica MD13G1048100.v1.1
prunus_persica Prupe.1G304000_v2.0.a1
pyrus_communis pycom13g04220
rosa_chinensis RchiOBHm_Chr4g0436011
rosa_laevigata RLG00000006542
rosa_roxburghii Rroxscaffold_5G00377160
rosa_rugosa Rorug04G0291200
rosa_samantha Rh4AG345500 Rh4BG354500 Rh4CG369000 Rh4DG348200
rosa_wichuraiana Rw4G030210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 639
AclWI GGATC 1 cut(s) 211
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 2 cut(s) 503, 589
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 5 cut(s) 100, 240, 250, 275, 323
AhlI ACTAGT 1 cut(s) 22
AjnI CCWGG 1 cut(s) 232
AluBI AGCT 5 cut(s) 71, 108, 158, 401, 426
AluI AGCT 5 cut(s) 71, 108, 158, 401, 426
Alw21I GWGCWC 1 cut(s) 403
AlwI GGATC 1 cut(s) 211
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 2 cut(s) 68, 656
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 466
BanII GRGCYC 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 263, 720
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 2 cut(s) 80, 643
BccI CCATC 3 cut(s) 364, 406, 592
BceAI ACGGC 2 cut(s) 457, 497
BciT130I CCWGG 1 cut(s) 234
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 3 cut(s) 352, 522, 657
BisI GCNGC 3 cut(s) 69, 640, 657
BlsI GCNGC 3 cut(s) 70, 641, 658
Bme1390I CCNGG 1 cut(s) 234
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 1 cut(s) 234
BmrI ACTGGG 1 cut(s) 629
BmsI GCATC 2 cut(s) 393, 442
BmuI ACTGGG 1 cut(s) 629
BpiI GAAGAC 2 cut(s) 263, 720
Bpu10I CCTNAGC 2 cut(s) 422, 461
BsaAI YACGTR 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 473, 487, 513
Bse1I ACTGG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 383
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 473, 487, 513
BseGI GGATG 4 cut(s) 163, 375, 381, 384
BseJI GATNNNNATC 1 cut(s) 383
BseMII CTCAG 3 cut(s) 66, 413, 416
BseNI ACTGG 1 cut(s) 624
BseRI GAGGAG 2 cut(s) 23, 112
BseXI GCAGC 2 cut(s) 80, 643
BshFI GGCC 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 403
BsiWI CGTACG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 630
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 512
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 2 cut(s) 216, 378
BspACI CCGC 1 cut(s) 639
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 3 cut(s) 65, 414, 415
BspLI GGNNCC 1 cut(s) 419
BspMAI CTGCAG 2 cut(s) 526, 661
BspPI GGATC 1 cut(s) 211
BspQI GCTCTTC 1 cut(s) 165
BsrI ACTGG 1 cut(s) 624
BssECI CCNNGG 3 cut(s) 473, 487, 513
BssMI GATC 2 cut(s) 216, 378
BssT1I CCWWGG 1 cut(s) 487
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 3 cut(s) 10, 506, 587
Bst6I CTCTTC 3 cut(s) 41, 165, 560
BstBAI YACGTR 1 cut(s) 501
BstC8I GCNNGC 2 cut(s) 60, 627
BstDEI CTNAG 5 cut(s) 52, 402, 422, 461, 732
BstDSI CCRYGG 2 cut(s) 473, 513
BstF5I GGATG 4 cut(s) 163, 375, 381, 384
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 2 cut(s) 219, 381
BstMBI GATC 2 cut(s) 216, 378
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 232
BstSFI CTRYAG 3 cut(s) 352, 522, 657
BstV1I GCAGC 2 cut(s) 80, 643
BstV2I GAAGAC 2 cut(s) 263, 720
BsuRI GGCC 1 cut(s) 512
BtgI CCRYGG 2 cut(s) 473, 513
BtsCI GGATG 4 cut(s) 163, 375, 381, 384
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 4 cut(s) 264, 410, 583, 666
Cac8I GCNNGC 2 cut(s) 60, 627
CfoI GCGC 1 cut(s) 450
Csp6I GTAC 2 cut(s) 502, 588
CviAII CATG 3 cut(s) 175, 280, 724
CviQI GTAC 2 cut(s) 502, 588
DdeI CTNAG 5 cut(s) 52, 402, 422, 461, 732
DpnI GATC 2 cut(s) 218, 380
DpnII GATC 2 cut(s) 216, 378
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 3 cut(s) 41, 165, 560
EarI CTCTTC 3 cut(s) 41, 165, 560
Ecl136II GAGCTC 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 487
Eco24I GRGCYC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 487
FaeI CATG 3 cut(s) 178, 283, 727
FaiI YATR 5 cut(s) 176, 222, 281, 354, 725
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 1 cut(s) 630
FatI CATG 3 cut(s) 174, 279, 723
Fnu4HI GCNGC 3 cut(s) 69, 640, 657
FokI GGATG 4 cut(s) 150, 371, 382, 388
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 3 cut(s) 69, 640, 657
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 449
GluI GCNGC 3 cut(s) 69, 640, 657
HaeIII GGCC 1 cut(s) 512
HhaI GCGC 1 cut(s) 450
Hin1II CATG 3 cut(s) 178, 283, 727
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 3 cut(s) 48, 492, 593
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 2 cut(s) 549, 692
Hpy188I TCNGA 1 cut(s) 709
Hpy188III TCNNGA 1 cut(s) 421
Hpy8I GTNNAC 2 cut(s) 549, 692
HpyAV CCTTC 4 cut(s) 27, 106, 122, 325
HpyCH4III ACNGT 3 cut(s) 10, 506, 587
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 8 cut(s) 62, 68, 262, 361, 384, 524, 659, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 5 cut(s) 52, 402, 422, 461, 732
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 3 cut(s) 178, 283, 727
HspAI GCGC 1 cut(s) 448
Kzo9I GATC 2 cut(s) 216, 378
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 1 cut(s) 398
Lsp1109I GCAGC 2 cut(s) 80, 643
LweI GCATC 2 cut(s) 393, 442
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 500
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 218, 380
MboI GATC 2 cut(s) 216, 378
MboII GAAGA 8 cut(s) 28, 152, 262, 263, 340, 475, 577, 720
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 4 cut(s) 210, 317, 346, 648
MlyI GAGTC 2 cut(s) 486, 602
MnlI CCTC 9 cut(s) 44, 126, 133, 155, 177, 398, 431, 450, 561
MspA1I CMGCKG 1 cut(s) 639
MspR9I CCNGG 1 cut(s) 234
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 68
NdeII GATC 2 cut(s) 216, 378
NlaIII CATG 3 cut(s) 178, 283, 727
NlaIV GGNNCC 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PctI GAATGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 48
Pfl23II CGTACG 1 cut(s) 501
PkrI GCNGC 3 cut(s) 70, 641, 658
PleI GAGTC 2 cut(s) 486, 601
PpsI GAGTC 2 cut(s) 486, 601
Ppu21I YACGTR 1 cut(s) 501
Psp124BI GAGCTC 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PspLI CGTACG 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 419
PstI CTGCAG 2 cut(s) 526, 661
RsaI GTAC 2 cut(s) 503, 589
RsaNI GTAC 2 cut(s) 502, 588
SacI GAGCTC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 165
SatI GCNGC 3 cut(s) 69, 640, 657
Sau3AI GATC 2 cut(s) 216, 378
SchI GAGTC 2 cut(s) 486, 602
ScrFI CCNGG 1 cut(s) 234
SduI GDGCHC 1 cut(s) 403
SetI ASST 8 cut(s) 19, 73, 110, 160, 227, 403, 428, 503
SfaNI GCATC 2 cut(s) 393, 442
SfcI CTRYAG 3 cut(s) 352, 522, 657
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 4 cut(s) 210, 317, 346, 648
SsiI CCGC 1 cut(s) 639
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 1 cut(s) 232
StyI CCWWGG 1 cut(s) 487
TaaI ACNGT 3 cut(s) 10, 506, 587
TaiI ACGT 1 cut(s) 503
TaqI TCGA 2 cut(s) 436, 596
TasI AATT 4 cut(s) 210, 317, 346, 648
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 1 cut(s) 48
TscAI CASTG 4 cut(s) 264, 410, 590, 666
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 68, 656
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 152, 163
TspRI CASTG 4 cut(s) 264, 410, 590, 666
XapI RAATTY 1 cut(s) 317
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.