pycom13g05520

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015607
Physical Location & Seq
Reverse (-)
3662148 .. 3663418
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGCCGCCGTCGGCCTCAGAAACCCTACTTCTCATCACCATTTTCTTCTTGCTTCCTCTCGACACCCTCTCATCCGTCATCCACGCAGGCCATGCCATCCCTCTCACCGGTATGTGTGAGGACGCATGCGGTGCAATCCCAGTAAAGTTCCCATTTGGTACCGGATTTGGTTGCGGCCACCCTGAATTTGTCAGATACATCAAATGCAGCTCAGGAACACTGCAATTCTCCACCGGAACGGGCATCTACACCATTTCCTCCATAGACTACAATAGCAGCACCATCATTGTCACAGATCCACTCATGTCAACATGTTCCTCCATGCAAAATTCAGGTAGTTTTAGCTTGGACAAGTCCAGTCCTTTCACTATAGGAGAGCAAAACATCTTCGTTTTGCTCGGCTGCTCAACGACATCCCCGGTGTTTGATCCAAACGAAGACTTGTGTGACACTGGATCCGGCACCCGGGTGTGTAGAGGTTTGTACTCTTGCAAGGGGGTGGCCGGCATTGGACTGCCACAGAATGCGCCGATTTCCACCTGTTGTGTTTATGATTCTGAACTCGTGGTTGGCTCAGGTTATGCATTGGATCTTCCAAAGCTCCAGTGCTCTTCTTATACATCAGTTTACGAGTTTGGAGACGAAGGCGATCCGACGAAATGGAAATTTGGGATTTCTTTGCAATATAATGATTCTTACCACACAAATAAATGCAAGGATTGTGAAACAAGTGGAGGGATTTGTGGATTTGCTGGTTTGGATCAGTCATTTGCTTGTATTTGCAGGAGTGGTGTTAACACCACCACAAATTGTTTTGGGCGAGGGTATGCTTGGAGTGGGGCAGTGGAACTGGATTTTCAAGTTAACACCATTATTGGAGGACTTTTGCTCTCGTGGGCGTTGCTATTTATATATAATCAGCAGATAGAGCTTCAAGACCTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

316

Amino Acids

33.66

Weight (kDa)

4.47

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 99 4.5e-10 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 224 - 263 3.4e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015607)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30030 FvH4_4g30030
malus_domestica MD13G1062800.v1.1
prunus_persica Prupe.1G286900_v2.0.a1
pyrus_communis pycom13g05520
rosa_chinensis RchiOBHm_Chr4g0438321
rosa_laevigata RLG00000006370
rosa_multiflora Rmu_sc0011129.1_g000001
rosa_roxburghii Rroxscaffold_5G00379410
rosa_rugosa Rorug04G0309000
rosa_samantha Rh4AG360100 Rh4BG371100 Rh4CG385100 Rh4DG366000
rosa_wichuraiana Rw0G006470 Rw0G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 158
AccB1I GGYRCC 2 cut(s) 158, 461
AciI CCGC 3 cut(s) 5, 129, 174
AclWI GGATC 7 cut(s) 290, 422, 450, 463, 597, 644, 768
AcoI YGGCCR 2 cut(s) 175, 501
AcsI RAATTY 3 cut(s) 185, 328, 665
AfaI GTAC 2 cut(s) 160, 485
AfiI CCNNNNNNNGG 2 cut(s) 107, 465
AflIII ACRYGT 1 cut(s) 311
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 2 cut(s) 860, 935
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 4 cut(s) 210, 345, 601, 931
AluI AGCT 4 cut(s) 210, 345, 601, 931
Alw21I GWGCWC 1 cut(s) 611
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 7 cut(s) 290, 422, 450, 463, 597, 644, 768
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 4 cut(s) 12, 88, 175, 501
ApeKI GCWGC 3 cut(s) 207, 276, 402
ApoI RAATTY 3 cut(s) 185, 328, 665
AsiGI ACCGGT 1 cut(s) 107
Asp718I GGTACC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 529
AsuC2I CCSGG 3 cut(s) 419, 466, 467
AsuHPI GGTGA 2 cut(s) 28, 97
AvaI CYCGRG 1 cut(s) 465
BamHI GGATCC 1 cut(s) 455
BanI GGYRCC 2 cut(s) 158, 461
BauI CACGAG 2 cut(s) 563, 892
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 611
BbvI GCAGC 3 cut(s) 219, 288, 389
BccI CCATC 2 cut(s) 104, 290
BcnI CCSGG 3 cut(s) 419, 466, 467
BcoDI GTCTC 1 cut(s) 633
BfmI CTRYAG 2 cut(s) 369, 944
BisI GCNGC 5 cut(s) 5, 175, 208, 277, 403
BlsI GCNGC 5 cut(s) 6, 176, 209, 278, 404
Bme1390I CCNGG 3 cut(s) 419, 466, 467
BmeT110I CYCGRG 1 cut(s) 465
BmiI GGNNCC 3 cut(s) 160, 457, 463
BmrFI CCNGG 3 cut(s) 419, 466, 467
BmrI ACTGGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 587
Bpu10I CCTNAGC 2 cut(s) 211, 574
BpuMI CCSGG 3 cut(s) 419, 466, 467
BsaJI CCNNGG 2 cut(s) 417, 465
BsaWI WCCGGW 3 cut(s) 107, 161, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 465
Bse118I RCCGGY 2 cut(s) 107, 503
Bse1I ACTGG 5 cut(s) 140, 357, 457, 604, 855
BseDI CCNNGG 2 cut(s) 417, 465
BseGI GGATG 4 cut(s) 71, 78, 96, 413
BseLI CCNNNNNNNGG 2 cut(s) 107, 465
BseMII CTCAG 3 cut(s) 30, 225, 588
BseNI ACTGG 5 cut(s) 140, 357, 457, 604, 855
BseXI GCAGC 3 cut(s) 219, 288, 389
BshFI GGCC 4 cut(s) 14, 90, 177, 503
BshNI GGYRCC 2 cut(s) 158, 461
BshTI ACCGGT 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 611
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 7 cut(s) 108, 162, 234, 419, 459, 466, 504
BslI CCNNNNNNNGG 2 cut(s) 107, 465
BsmAI GTCTC 1 cut(s) 633
BsmBI CGTCTC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 4 cut(s) 14, 90, 177, 503
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 611
Bsp143I GATC 6 cut(s) 295, 427, 455, 589, 649, 760
BspACI CCGC 3 cut(s) 5, 129, 174
BspANI GGCC 4 cut(s) 14, 90, 177, 503
BspCNI CTCAG 3 cut(s) 29, 224, 587
BspLI GGNNCC 3 cut(s) 160, 457, 463
BspPI GGATC 7 cut(s) 290, 422, 450, 463, 597, 644, 768
BspQI GCTCTTC 1 cut(s) 616
BspT107I GGYRCC 2 cut(s) 158, 461
BsrFI RCCGGY 2 cut(s) 107, 503
BsrI ACTGG 5 cut(s) 140, 357, 457, 604, 855
BssAI RCCGGY 2 cut(s) 107, 503
BssECI CCNNGG 2 cut(s) 417, 465
BssMI GATC 6 cut(s) 295, 427, 455, 589, 649, 760
BssSI CACGAG 2 cut(s) 563, 892
Bst2BI CACGAG 2 cut(s) 563, 892
Bst6I CTCTTC 1 cut(s) 616
BstAPI GCANNNNNTGC 2 cut(s) 92, 131
BstC8I GCNNGC 3 cut(s) 88, 127, 505
BstDEI CTNAG 3 cut(s) 16, 211, 574
BstF5I GGATG 4 cut(s) 71, 78, 96, 413
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 6 cut(s) 298, 430, 458, 592, 652, 763
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 6 cut(s) 295, 427, 455, 589, 649, 760
BstMWI GCNNNNNNNGC 3 cut(s) 92, 131, 928
BstNSI RCATGY 2 cut(s) 129, 315
BstSCI CCNGG 3 cut(s) 417, 464, 465
BstSFI CTRYAG 2 cut(s) 369, 944
BstV1I GCAGC 3 cut(s) 219, 288, 389
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 3 cut(s) 295, 455, 589
BstYI RGATCY 3 cut(s) 295, 455, 589
BsuRI GGCC 4 cut(s) 14, 90, 177, 503
BtsCI GGATG 4 cut(s) 71, 78, 96, 413
BtsI GCAGTG 2 cut(s) 218, 849
BtsIMutI CAGTG 4 cut(s) 218, 450, 611, 849
Cac8I GCNNGC 3 cut(s) 88, 127, 505
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 2 cut(s) 107, 503
Cfr9I CCCGGG 1 cut(s) 465
CseI GACGC 1 cut(s) 131
Csp6I GTAC 2 cut(s) 159, 484
CspAI ACCGGT 1 cut(s) 107
CviAII CATG 5 cut(s) 92, 126, 304, 312, 322
CviQI GTAC 2 cut(s) 159, 484
DdeI CTNAG 3 cut(s) 16, 211, 574
DpnI GATC 6 cut(s) 297, 429, 457, 591, 651, 762
DpnII GATC 6 cut(s) 295, 427, 455, 589, 649, 760
EaeI YGGCCR 2 cut(s) 175, 501
Eam1104I CTCTTC 1 cut(s) 616
EarI CTCTTC 1 cut(s) 616
Eco88I CYCGRG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 586
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 5 cut(s) 95, 129, 307, 315, 325
FatI CATG 5 cut(s) 91, 125, 303, 311, 321
Fnu4HI GCNGC 5 cut(s) 5, 175, 208, 277, 403
FokI GGATG 4 cut(s) 58, 65, 83, 400
Fsp4HI GCNGC 5 cut(s) 5, 175, 208, 277, 403
GlaI GCGC 1 cut(s) 528
GluI GCNGC 5 cut(s) 5, 175, 208, 277, 403
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 4 cut(s) 14, 90, 177, 503
HapII CCGG 7 cut(s) 108, 162, 234, 419, 459, 466, 504
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 529
Hin1II CATG 5 cut(s) 95, 129, 307, 315, 325
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HincII GTYRAC 3 cut(s) 309, 796, 865
HindII GTYRAC 3 cut(s) 309, 796, 865
HinfI GANTC 2 cut(s) 554, 692
HpaI GTTAAC 2 cut(s) 796, 865
HpaII CCGG 7 cut(s) 108, 162, 234, 419, 459, 466, 504
HphI GGTGA 2 cut(s) 28, 97
Hpy166II GTNNAC 4 cut(s) 309, 628, 796, 865
Hpy188I TCNGA 4 cut(s) 19, 194, 559, 654
Hpy188III TCNNGA 3 cut(s) 59, 213, 935
Hpy8I GTNNAC 4 cut(s) 309, 628, 796, 865
Hpy99I CGWCG 2 cut(s) 13, 658
HpyAV CCTTC 1 cut(s) 638
HpyCH4V TGCA 9 cut(s) 134, 207, 223, 325, 492, 584, 682, 714, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 131, 928
HpyF3I CTNAG 3 cut(s) 16, 211, 574
Hsp92II CATG 5 cut(s) 95, 129, 307, 315, 325
HspAI GCGC 1 cut(s) 527
KpnI GGTACC 1 cut(s) 162
KroI GCCGGC 1 cut(s) 503
KroNI GCCGGC 1 cut(s) 505
KspAI GTTAAC 2 cut(s) 796, 865
Kzo9I GATC 6 cut(s) 295, 427, 455, 589, 649, 760
LguI GCTCTTC 1 cut(s) 616
LmnI GCTCC 1 cut(s) 606
Lsp1109I GCAGC 3 cut(s) 219, 288, 389
LweI GCATC 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 289, 446
MalI GATC 6 cut(s) 297, 429, 457, 591, 651, 762
MboI GATC 6 cut(s) 295, 427, 455, 589, 649, 760
MboII GAAGA 5 cut(s) 37, 379, 449, 584, 603
MflI RGATCY 3 cut(s) 295, 455, 589
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 5 cut(s) 185, 224, 328, 665, 808
MmeI TCCRAC 1 cut(s) 677
Mph1103I ATGCAT 1 cut(s) 586
MroNI GCCGGC 1 cut(s) 503
MseI TTAA 2 cut(s) 795, 864
MslI CAYNNNNRTG 2 cut(s) 110, 467
MspI CCGG 7 cut(s) 108, 162, 234, 419, 459, 466, 504
MspR9I CCNGG 3 cut(s) 419, 466, 467
Mva1269I GAATGC 1 cut(s) 529
MwoI GCNNNNNNNGC 3 cut(s) 92, 131, 928
NaeI GCCGGC 1 cut(s) 505
NciI CCSGG 3 cut(s) 419, 466, 467
NdeII GATC 6 cut(s) 295, 427, 455, 589, 649, 760
NgoMIV GCCGGC 1 cut(s) 503
NlaIII CATG 5 cut(s) 95, 129, 307, 315, 325
NlaIV GGNNCC 3 cut(s) 160, 457, 463
NmeAIII GCCGAG 1 cut(s) 378
NmuCI GTSAC 2 cut(s) 289, 446
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 2 cut(s) 129, 315
OliI CACNNNNGTG 1 cut(s) 467
PaeI GCATGC 1 cut(s) 129
PciI ACATGT 1 cut(s) 311
PciSI GCTCTTC 1 cut(s) 616
PctI GAATGC 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 505
PfeI GAWTC 2 cut(s) 554, 692
PinAI ACCGGT 1 cut(s) 107
PkrI GCNGC 5 cut(s) 6, 176, 209, 278, 404
PscI ACATGT 1 cut(s) 311
PspN4I GGNNCC 3 cut(s) 160, 457, 463
PsuI RGATCY 3 cut(s) 295, 455, 589
RsaI GTAC 2 cut(s) 160, 485
RsaNI GTAC 2 cut(s) 159, 484
RseI CAYNNNNRTG 2 cut(s) 110, 467
SapI GCTCTTC 1 cut(s) 616
SaqAI TTAA 2 cut(s) 795, 864
SatI GCNGC 5 cut(s) 5, 175, 208, 277, 403
Sau3AI GATC 6 cut(s) 295, 427, 455, 589, 649, 760
ScrFI CCNGG 3 cut(s) 419, 466, 467
SduI GDGCHC 1 cut(s) 611
SetI ASST 9 cut(s) 212, 337, 347, 481, 542, 580, 603, 933, 942
SfaNI GCATC 1 cut(s) 252
SfcI CTRYAG 2 cut(s) 369, 944
SmaI CCCGGG 1 cut(s) 467
SmiMI CAYNNNNRTG 2 cut(s) 110, 467
SphI GCATGC 1 cut(s) 129
Sse9I AATT 5 cut(s) 185, 224, 328, 665, 808
SsiI CCGC 3 cut(s) 5, 129, 174
StyD4I CCNGG 3 cut(s) 417, 464, 465
TaqI TCGA 1 cut(s) 60
TasI AATT 5 cut(s) 185, 224, 328, 665, 808
TatI WGTACW 1 cut(s) 483
TauI GCSGC 2 cut(s) 7, 177
TfiI GAWTC 2 cut(s) 554, 692
Tru1I TTAA 2 cut(s) 795, 864
Tru9I TTAA 2 cut(s) 795, 864
TscAI CASTG 4 cut(s) 225, 457, 611, 849
TseFI GTSAC 2 cut(s) 289, 446
TseI GCWGC 3 cut(s) 207, 276, 402
Tsp45I GTSAC 2 cut(s) 289, 446
TspGWI ACGGA 1 cut(s) 64
TspMI CCCGGG 1 cut(s) 465
TspRI CASTG 4 cut(s) 225, 457, 611, 849
XapI RAATTY 3 cut(s) 185, 328, 665
XceI RCATGY 2 cut(s) 129, 315
XmaI CCCGGG 1 cut(s) 465
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.