pycom13g16470
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0019828
Physical Location & Seq
Forward (+)
12259993 .. 12260556
564 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16470.1

Sequence Viewer

Length: 564 bp
ATGAGCATTTGTAAAAAGGAGGAGCTGTACAAAGGGATGAACCAGTTGGAAGCTGCTGTCACTGGAGCGGCACTAGAAGGAGCGGTGGCATCAGGTCTTAGCGATCCCTTTGGTAGTTTGGACTTGCTTACCATTCAAGCCACCCCTCTCGCCATTGGGATTCGAGCTGATGGAAACAACTTTGTGCCTATCATACCTAGAAACACAGCAATGCCAGCACAAAAAGATATGATTTTCACAACTGCCCATGATAATCAAACTGAAGCACTAATAATCGTCCACGAAGGCGAGGGGAAGAAGGTGGAAGAGAACCATTTGCTGGGGTATTTCAAGATCACTGGAATTCCACCATCCCCGAAAGGAGTTCCGGAAATAAGAATAATTCTGGACATCGATGCATCGAGCGTGCTGAGAGTTTTATCGGGGGTTTTAATGCCGGGGTCTCATCAGCCGGTAAATCCAGTCATGGGAGTGAGGATGCCAACAGTTGATGATGGTCACGGCTGGTGTGCCCAAGCCCTACACAGAACTTATGGTTCTACTCTCGACTTGGTTACAGTGTAG

Protein Analysis

188

Amino Acids

19.93

Weight (kDa)

5.43

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 7 - 142 3.5e-30 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019828)

Species Orthologous Gene IDs
pyrus_communis pycom13g16470
rosa_chinensis RchiOBHm_Chr6g0290311
rosa_multiflora Rmu_sc0015642.1_g000001
rosa_roxburghii Rroxscaffold_7G00177170
rosa_samantha Rh6AG322000 Rh6CG335200
rosa_wichuraiana Rw6G027900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 319
AccBSI CCGCTC 2 cut(s) 68, 83
AccIII TCCGGA 1 cut(s) 367
AciI CCGC 2 cut(s) 68, 83
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 282
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 319, 467
AgsI TTSAA 2 cut(s) 137, 331
AluBI AGCT 3 cut(s) 25, 53, 167
AluI AGCT 3 cut(s) 25, 53, 167
Alw26I GTCTC 1 cut(s) 447
AlwI GGATC 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 367
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 514
BbvI GCAGC 1 cut(s) 40
BccI CCATC 3 cut(s) 164, 358, 488
BceAI ACGGC 1 cut(s) 517
BcnI CCSGG 1 cut(s) 438
BcoDI GTCTC 1 cut(s) 447
BfaI CTAG 2 cut(s) 74, 198
BisI GCNGC 2 cut(s) 54, 69
BlsI GCNGC 2 cut(s) 55, 70
Bme1390I CCNGG 1 cut(s) 438
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 4 cut(s) 98, 385, 407, 468
BpmI CTGGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 438
Bsa29I ATCGAT 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 437
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 467
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 4 cut(s) 43, 67, 343, 461
Bse3DI GCAATG 1 cut(s) 216
BseAI TCCGGA 1 cut(s) 367
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 3 cut(s) 42, 350, 483
BseLI CCNNNNNNNGG 2 cut(s) 319, 467
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 4 cut(s) 43, 67, 343, 461
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 514
BseXI GCAGC 1 cut(s) 40
BseYI CCCAGC 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 393
BsiSI CCGG 3 cut(s) 368, 437, 452
BslI CCNNNNNNNGG 2 cut(s) 319, 467
BsmAI GTCTC 1 cut(s) 447
Bso31I GGTCTC 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 514
Bsp13I TCCGGA 1 cut(s) 367
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 2 cut(s) 103, 333
BspACI CCGC 2 cut(s) 68, 83
BspCNI CTCAG 1 cut(s) 402
BspDI ATCGAT 1 cut(s) 393
BspEI TCCGGA 1 cut(s) 367
BspPI GGATC 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 447
BsrBI CCGCTC 2 cut(s) 68, 83
BsrDI GCAATG 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 451
BsrGI TGTACA 1 cut(s) 27
BsrI ACTGG 4 cut(s) 43, 67, 343, 461
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 2 cut(s) 103, 333
Bst4CI ACNGT 2 cut(s) 487, 559
Bst6I CTCTTC 1 cut(s) 300
BstAUI TGTACA 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 216, 407
BstDEI CTNAG 2 cut(s) 98, 410
BstF5I GGATG 3 cut(s) 42, 350, 483
BstKTI GATC 2 cut(s) 106, 336
BstMAI GTCTC 1 cut(s) 447
BstMBI GATC 2 cut(s) 103, 333
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 436
BstSLI GKGCMC 1 cut(s) 514
BstV1I GCAGC 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 393
BsuTUI ATCGAT 1 cut(s) 393
BtsCI GGATG 3 cut(s) 42, 350, 483
BtsIMutI CAGTG 3 cut(s) 60, 336, 564
Cac8I GCNNGC 2 cut(s) 216, 407
Cfr10I RCCGGY 1 cut(s) 451
ClaI ATCGAT 1 cut(s) 393
Csp6I GTAC 1 cut(s) 28
CviAII CATG 2 cut(s) 248, 466
CviJI RGCY 7 cut(s) 25, 53, 140, 167, 451, 504, 518
CviKI_1 RGCY 7 cut(s) 25, 53, 140, 167, 451, 504, 518
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 2 cut(s) 98, 410
DpnI GATC 2 cut(s) 105, 335
DpnII GATC 2 cut(s) 103, 333
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco31I GGTCTC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 400
FaeI CATG 2 cut(s) 251, 469
FaiI YATR 5 cut(s) 194, 230, 249, 467, 534
FatI CATG 2 cut(s) 247, 465
Fnu4HI GCNGC 2 cut(s) 54, 69
FokI GGATG 3 cut(s) 49, 337, 490
Fsp4HI GCNGC 2 cut(s) 54, 69
FspBI CTAG 2 cut(s) 74, 198
GluI GCNGC 2 cut(s) 54, 69
GsaI CCCAGC 1 cut(s) 323
GsuI CTGGAG 1 cut(s) 84
HapII CCGG 3 cut(s) 368, 437, 452
Hin1II CATG 2 cut(s) 251, 469
HinfI GANTC 1 cut(s) 160
HpaII CCGG 3 cut(s) 368, 437, 452
Hpy166II GTNNAC 1 cut(s) 280
Hpy188III TCNNGA 4 cut(s) 331, 368, 386, 545
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 3 cut(s) 71, 278, 292
HpyCH4III ACNGT 2 cut(s) 487, 559
HpyCH4V TGCA 1 cut(s) 398
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 2 cut(s) 98, 410
Hsp92II CATG 2 cut(s) 251, 469
Kpn2I TCCGGA 1 cut(s) 367
Kzo9I GATC 2 cut(s) 103, 333
LmnI GCTCC 3 cut(s) 22, 65, 80
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 4 cut(s) 98, 385, 407, 468
MaeI CTAG 2 cut(s) 74, 198
MaeIII GTNAC 3 cut(s) 58, 497, 553
MalI GATC 2 cut(s) 105, 335
MbiI CCGCTC 2 cut(s) 68, 83
MboI GATC 2 cut(s) 103, 333
MboII GAAGA 2 cut(s) 307, 317
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 2 cut(s) 342, 381
MmeI TCCRAC 1 cut(s) 27
MnlI CCTC 4 cut(s) 13, 156, 283, 468
Mph1103I ATGCAT 1 cut(s) 400
MroI TCCGGA 1 cut(s) 367
MseI TTAA 1 cut(s) 431
MslI CAYNNNNRTG 2 cut(s) 209, 470
MspI CCGG 3 cut(s) 368, 437, 452
MspR9I CCNGG 1 cut(s) 438
MwoI GCNNNNNNNGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 438
NdeII GATC 2 cut(s) 103, 333
NlaIII CATG 2 cut(s) 251, 469
NmuCI GTSAC 2 cut(s) 58, 497
NsiI ATGCAT 1 cut(s) 400
PfeI GAWTC 1 cut(s) 160
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 2 cut(s) 55, 70
PspFI CCCAGC 1 cut(s) 319
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 2 cut(s) 209, 470
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 2 cut(s) 54, 69
Sau3AI GATC 2 cut(s) 103, 333
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 514
SetI ASST 6 cut(s) 27, 55, 97, 169, 199, 303
SfaNI GCATC 4 cut(s) 98, 385, 407, 468
SmiMI CAYNNNNRTG 2 cut(s) 209, 470
Sse9I AATT 2 cut(s) 342, 381
SsiI CCGC 2 cut(s) 68, 83
SspMI CTAG 2 cut(s) 74, 198
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 487, 559
TaqI TCGA 4 cut(s) 163, 393, 401, 546
TasI AATT 2 cut(s) 342, 381
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 3 cut(s) 67, 343, 564
TseFI GTSAC 2 cut(s) 58, 497
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 2 cut(s) 58, 497
TspDTI ATGAA 1 cut(s) 53
TspRI CASTG 3 cut(s) 67, 343, 564
Van91I CCANNNNNTGG 1 cut(s) 319
XapI RAATTY 1 cut(s) 342
XspI CTAG 2 cut(s) 74, 198
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.