Rroxscaffold_7G00177170
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0019828
Physical Location & Seq
Reverse (-)
16702344 .. 16705996
3653 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00177170.1

Sequence Viewer

Length: 573 bp
ATGAGCGTATGCAAGAAAGAGGAGCTTTACGAAGGGATGAACCCATTGGAATCTGCTGTTTCTGGAGCAGCACTACAAGGATATGTAGCTTCAGGTGTTCATGATCCATTTGAATCCGTGGATGTGCTATCTATTCAGGTCACACCTCTCTCGATTGGAATTCAAGCTGATGGAAACAACTTTGTGCCGATCAAACCTAGAAACACCACAATACCAGCCCAAAGAGAGATGCTTTTCACAACATCCCATGATAATCAAGCCAAAGCACTTGTAATTGTTTATGAAGGTGATGGCAAGAAGCTGGAAGAAAATCATTTGCTGGGATACTTCAAAATTGAGGGAATTCCTCCAGCAACCAAGGGAGTTCCACAAATTCGAGTAATGATGGACATTGATGCTTCAAACGTGCTGAGAGTTTTGGCTGCTGTGTTGATGCCAGGTTCTCATCTTCCAATAAATCCTGTCCTGGACGTGAGGATGCCAACGGTTGACGATGGTCATGCCTGGTGCGCGGAAGCCTTGCACAGAACTTATGGTGCTACTCAAGACTTGGTATCAGTGAAGAGGATTTAG

Protein Analysis

190

Amino Acids

20.63

Weight (kDa)

5.46

Isoelectric Point (pI)

25.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 7 - 144 4.3e-31 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019828)

Species Orthologous Gene IDs
pyrus_communis pycom13g16470
rosa_chinensis RchiOBHm_Chr6g0290311
rosa_multiflora Rmu_sc0015642.1_g000001
rosa_roxburghii Rroxscaffold_7G00177170
rosa_samantha Rh6AG322000 Rh6CG335200
rosa_wichuraiana Rw6G027900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 512
AciI CCGC 1 cut(s) 512
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 3 cut(s) 159, 342, 372
AcuI CTGAAG 1 cut(s) 75
AgsI TTSAA 4 cut(s) 113, 164, 331, 402
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 3 cut(s) 436, 465, 503
AluBI AGCT 4 cut(s) 25, 89, 167, 301
AluI AGCT 4 cut(s) 25, 89, 167, 301
AlwI GGATC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 68, 422
ApoI RAATTY 3 cut(s) 159, 342, 372
AspLEI GCGC 1 cut(s) 512
AsuHPI GGTGA 1 cut(s) 299
BbvI GCAGC 2 cut(s) 80, 409
BccI CCATC 4 cut(s) 164, 284, 379, 488
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 3 cut(s) 438, 467, 505
BciVI GTATCC 1 cut(s) 317
BfaI CTAG 1 cut(s) 198
BfuI GTATCC 1 cut(s) 317
BisI GCNGC 2 cut(s) 69, 423
BlsI GCNGC 2 cut(s) 70, 424
Bme1390I CCNGG 3 cut(s) 438, 467, 505
BmgBI CACGTC 1 cut(s) 472
BmrFI CCNGG 3 cut(s) 438, 467, 505
BmsI GCATC 4 cut(s) 219, 385, 423, 468
BoxI GACNNNNGTC 1 cut(s) 495
BpmI CTGGAG 2 cut(s) 84, 333
BpuEI CTTGAG 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 117, 357
BseBI CCWGG 3 cut(s) 438, 467, 505
BseDI CCNNGG 2 cut(s) 117, 357
BseGI GGATG 4 cut(s) 42, 127, 242, 483
BseMII CTCAG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 80, 409
BseYI CCCAGC 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 512
Bsp143I GATC 2 cut(s) 103, 189
BspACI CCGC 1 cut(s) 512
BspCNI CTCAG 1 cut(s) 402
BspFNI CGCG 1 cut(s) 512
BspHI TCATGA 1 cut(s) 100
BspPI GGATC 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 117, 357
BssMI GATC 2 cut(s) 103, 189
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 3 cut(s) 438, 467, 505
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 1 cut(s) 557
BstDEI CTNAG 1 cut(s) 410
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 4 cut(s) 42, 127, 242, 483
BstFNI CGCG 1 cut(s) 512
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 2 cut(s) 106, 192
BstMBI GATC 2 cut(s) 103, 189
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 3 cut(s) 438, 467, 505
BstPAI GACNNNNGTC 1 cut(s) 495
BstSCI CCNGG 3 cut(s) 436, 465, 503
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 2 cut(s) 80, 409
BsuI GTATCC 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 117
BtrI CACGTC 1 cut(s) 472
BtsCI GGATG 4 cut(s) 42, 127, 242, 483
BtsIMutI CAGTG 1 cut(s) 564
CciI TCATGA 1 cut(s) 100
CfoI GCGC 1 cut(s) 512
CviAII CATG 3 cut(s) 101, 248, 500
CviJI RGCY 8 cut(s) 25, 89, 167, 218, 260, 301, 422, 518
CviKI_1 RGCY 8 cut(s) 25, 89, 167, 218, 260, 301, 422, 518
DdeI CTNAG 1 cut(s) 410
DpnI GATC 2 cut(s) 105, 191
DpnII GATC 2 cut(s) 103, 189
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Eco130I CCWWGG 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 75
EcoRI GAATTC 2 cut(s) 159, 342
EcoRII CCWGG 3 cut(s) 436, 465, 503
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 3 cut(s) 104, 251, 503
FaiI YATR 7 cut(s) 10, 84, 102, 249, 282, 501, 534
FalI AAGNNNNNCTT 2 cut(s) 9, 41
FatI CATG 3 cut(s) 100, 247, 499
Fnu4HI GCNGC 2 cut(s) 69, 423
FokI GGATG 4 cut(s) 49, 134, 229, 490
Fsp4HI GCNGC 2 cut(s) 69, 423
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 511
GluI GCNGC 2 cut(s) 69, 423
GsaI CCCAGC 1 cut(s) 323
GsuI CTGGAG 2 cut(s) 84, 333
HhaI GCGC 1 cut(s) 512
Hin1II CATG 3 cut(s) 104, 251, 503
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 2 cut(s) 50, 113
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 1 cut(s) 490
Hpy188III TCNNGA 4 cut(s) 63, 101, 151, 545
Hpy8I GTNNAC 1 cut(s) 490
HpyAV CCTTC 2 cut(s) 26, 278
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 2 cut(s) 405, 471
HpyCH4V TGCA 2 cut(s) 12, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 1 cut(s) 410
HpySE526I ACGT 2 cut(s) 405, 471
Hsp92II CATG 3 cut(s) 104, 251, 503
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 2 cut(s) 103, 189
LmnI GCTCC 2 cut(s) 22, 65
Lsp1109I GCAGC 2 cut(s) 80, 409
LweI GCATC 4 cut(s) 219, 385, 423, 468
MaeI CTAG 1 cut(s) 198
MaeII ACGT 2 cut(s) 405, 471
MaeIII GTNAC 1 cut(s) 139
MalI GATC 2 cut(s) 105, 191
MboI GATC 2 cut(s) 103, 189
MboII GAAGA 2 cut(s) 317, 440
MluCI AATT 5 cut(s) 159, 273, 333, 342, 372
MnlI CCTC 6 cut(s) 13, 156, 331, 357, 468, 558
MspR9I CCNGG 3 cut(s) 438, 467, 505
MvaI CCWGG 3 cut(s) 438, 467, 505
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 509
NdeII GATC 2 cut(s) 103, 189
NlaIII CATG 3 cut(s) 104, 251, 503
NmuCI GTSAC 1 cut(s) 139
PagI TCATGA 1 cut(s) 100
PfeI GAWTC 2 cut(s) 50, 113
PfoI TCCNGGA 1 cut(s) 465
PkrI GCNGC 2 cut(s) 70, 424
PshAI GACNNNNGTC 1 cut(s) 495
Psp6I CCWGG 3 cut(s) 436, 465, 503
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 3 cut(s) 436, 465, 503
SatI GCNGC 2 cut(s) 69, 423
Sau3AI GATC 2 cut(s) 103, 189
ScrFI CCNGG 3 cut(s) 438, 467, 505
SfaNI GCATC 4 cut(s) 219, 385, 423, 468
SmlI CTYRAG 1 cut(s) 543
SmoI CTYRAG 1 cut(s) 543
Sse9I AATT 5 cut(s) 159, 273, 333, 342, 372
SsiI CCGC 1 cut(s) 512
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 3 cut(s) 436, 465, 503
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 2 cut(s) 408, 474
TaqI TCGA 2 cut(s) 152, 376
TasI AATT 5 cut(s) 159, 273, 333, 342, 372
TfiI GAWTC 2 cut(s) 50, 113
TscAI CASTG 1 cut(s) 564
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 2 cut(s) 68, 422
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 3 cut(s) 53, 89, 297
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 1 cut(s) 564
XapI RAATTY 3 cut(s) 159, 342, 372
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.