Rw6G027900
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0019828
Physical Location & Seq
Forward (+)
51869509 .. 51870539
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027900.1

Sequence Viewer

Length: 939 bp
ATGGGTGCAGGATACTGTGATGTTGCCATAAGTGTGACAGCTGAAGAGGCACCACGAGGAATTTCTGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGACGAAATCAAATCAAAGGAGTTACTTCGAGTTGCAACACAGAATGCAATCCAGAGTCTCTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTAGTGGACCGAGAAGAATTTGAGGAAGTTAATCGTGCGGTATTTAATAAGTGTGTTAGCCTCATAATCCAGTGTTTGCATGATGCAAAGAAAGAGGAGCTTTACGAAGGGATGAACCCATTGGAAGCTGCTGTCTCTGGAGCAGCACTACAAGGATACTTAGCTTCAGGTGTTCATGATCCTTTTGAATCCATGGATGTGCTATCTATTCAGATCACCCCTCTCTCGATTGGAATTCAAGCTGATGGAAACAACTTTGTGCCGATCATACCTAGAAACACCACAATACCAGCCCAAAGAGAGATGCTTTTCACAACATCCCATGATAATCAAGCCGAAGCACTTATAATTGTTTATGAAGGTGATGGCAAGAAGCTGGAAGAAAATCATTTGCTGGGATACTGCAAAATTGAGGGAATTCCTCCAGCAACCAAGGGAGTTCCACAAATACGAGTAATGATGGACATTGATGCTTCAAACGTGCTGAGAGTTTTGGCTGCTGTGTTGATGCCAGGTTCTCATCTTCCAATAAATCCTGTCCTGGACGTGAGGATGCCAACGGTTGTCGATGATCATGCCTGGTGGGCGGAAGCCTTGCACAGAACTTATGGTACTACTCAAGGCTTGGTATCAGTGAAGAGGATCTAG

Protein Analysis

312

Amino Acids

33.93

Weight (kDa)

4.93

Isoelectric Point (pI)

24.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 130 - 266 9.6e-30 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019828)

Species Orthologous Gene IDs
pyrus_communis pycom13g16470
rosa_chinensis RchiOBHm_Chr6g0290311
rosa_multiflora Rmu_sc0015642.1_g000001
rosa_roxburghii Rroxscaffold_7G00177170
rosa_samantha Rh6AG322000 Rh6CG335200
rosa_wichuraiana Rw6G027900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 2 cut(s) 329, 878
AclWI GGATC 1 cut(s) 464
AcsI RAATTY 5 cut(s) 60, 241, 308, 525, 708
AcuI CTGAAG 3 cut(s) 63, 98, 441
AfaI GTAC 1 cut(s) 904
AgsI TTSAA 4 cut(s) 251, 479, 530, 768
AjiI CACGTC 1 cut(s) 838
AjnI CCWGG 3 cut(s) 802, 831, 869
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 6 cut(s) 41, 391, 419, 455, 533, 667
AluI AGCT 6 cut(s) 41, 391, 419, 455, 533, 667
Alw26I GTCTC 2 cut(s) 236, 430
AlwI GGATC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 419, 434, 788
ApoI RAATTY 5 cut(s) 60, 241, 308, 525, 708
AspS9I GGNCC 1 cut(s) 298
AsuHPI GGTGA 2 cut(s) 499, 665
AvaII GGWCC 1 cut(s) 298
BanI GGYRCC 1 cut(s) 49
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 406, 446, 775
BccI CCATC 3 cut(s) 530, 650, 745
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 3 cut(s) 804, 833, 871
BciVI GTATCC 3 cut(s) 5, 440, 683
BclI TGATCA 1 cut(s) 862
BcoDI GTCTC 2 cut(s) 236, 430
BfaI CTAG 2 cut(s) 564, 937
BfuI GTATCC 3 cut(s) 5, 440, 683
BglI GCCNNNNNGGC 1 cut(s) 875
BisI GCNGC 3 cut(s) 420, 435, 789
BlsI GCNGC 3 cut(s) 421, 436, 790
Bme1390I CCNGG 3 cut(s) 804, 833, 871
Bme18I GGWCC 1 cut(s) 298
BmgBI CACGTC 1 cut(s) 838
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 3 cut(s) 804, 833, 871
BmsI GCATC 6 cut(s) 114, 364, 585, 751, 789, 834
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 2 cut(s) 450, 699
BpuEI CTTGAG 1 cut(s) 894
BsaBI GATNNNNATC 1 cut(s) 281
BsaJI CCNNGG 2 cut(s) 483, 723
Bse1I ACTGG 1 cut(s) 361
Bse8I GATNNNNATC 1 cut(s) 281
BseBI CCWGG 3 cut(s) 804, 833, 871
BseDI CCNNGG 2 cut(s) 483, 723
BseGI GGATG 5 cut(s) 282, 408, 493, 608, 849
BseJI GATNNNNATC 1 cut(s) 281
BseMII CTCAG 1 cut(s) 767
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 2 cut(s) 114, 401
BseXI GCAGC 3 cut(s) 406, 446, 775
BseYI CCCAGC 1 cut(s) 685
BsgI GTGCAG 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 49
BsmAI GTCTC 2 cut(s) 236, 430
BsmI GAATGC 1 cut(s) 223
Bsp143I GATC 5 cut(s) 469, 504, 555, 862, 933
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 2 cut(s) 329, 878
BspCNI CTCAG 1 cut(s) 768
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 2 cut(s) 483, 723
BssMI GATC 5 cut(s) 469, 504, 555, 862, 933
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 483, 723
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 3 cut(s) 804, 833, 871
Bst4CI ACNGT 2 cut(s) 17, 853
Bst6I CTCTTC 2 cut(s) 39, 923
BstDEI CTNAG 2 cut(s) 451, 776
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 5 cut(s) 282, 408, 493, 608, 849
BstKTI GATC 5 cut(s) 472, 507, 558, 865, 936
BstMAI GTCTC 2 cut(s) 236, 430
BstMBI GATC 5 cut(s) 469, 504, 555, 862, 933
BstMWI GCNNNNNNNGC 2 cut(s) 47, 875
BstNI CCWGG 3 cut(s) 804, 833, 871
BstSCI CCNGG 3 cut(s) 802, 831, 869
BstV1I GCAGC 3 cut(s) 406, 446, 775
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 933
BstYI RGATCY 1 cut(s) 933
BsuI GTATCC 3 cut(s) 5, 440, 683
BtgI CCRYGG 1 cut(s) 483
BtrI CACGTC 1 cut(s) 838
BtsCI GGATG 5 cut(s) 282, 408, 493, 608, 849
BtsIMutI CAGTG 2 cut(s) 368, 930
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 298
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 903
CviAII CATG 6 cut(s) 167, 371, 467, 484, 614, 866
CviQI GTAC 1 cut(s) 903
DdeI CTNAG 2 cut(s) 451, 776
DpnI GATC 5 cut(s) 471, 506, 557, 864, 935
DpnII GATC 5 cut(s) 469, 504, 555, 862, 933
Eam1104I CTCTTC 2 cut(s) 39, 923
EarI CTCTTC 2 cut(s) 39, 923
EciI GGCGGA 1 cut(s) 893
Eco130I CCWWGG 2 cut(s) 483, 723
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 3 cut(s) 63, 98, 441
EcoRI GAATTC 2 cut(s) 525, 708
EcoRII CCWGG 3 cut(s) 802, 831, 869
EcoT14I CCWWGG 2 cut(s) 483, 723
ErhI CCWWGG 2 cut(s) 483, 723
FaeI CATG 6 cut(s) 170, 374, 470, 487, 617, 869
FalI AAGNNNNNCTT 4 cut(s) 96, 128, 375, 407
FatI CATG 6 cut(s) 166, 370, 466, 483, 613, 865
FbaI TGATCA 1 cut(s) 862
Fnu4HI GCNGC 3 cut(s) 420, 435, 789
FokI GGATG 5 cut(s) 289, 415, 500, 595, 856
Fsp4HI GCNGC 3 cut(s) 420, 435, 789
FspBI CTAG 2 cut(s) 564, 937
GluI GCNGC 3 cut(s) 420, 435, 789
GsaI CCCAGC 1 cut(s) 689
GsuI CTGGAG 2 cut(s) 450, 699
HgaI GACGC 1 cut(s) 116
Hin1II CATG 6 cut(s) 170, 374, 470, 487, 617, 869
HincII GTYRAC 2 cut(s) 175, 262
HindII GTYRAC 2 cut(s) 175, 262
HinfI GANTC 3 cut(s) 151, 229, 479
HphI GGTGA 2 cut(s) 499, 665
Hpy166II GTNNAC 3 cut(s) 175, 262, 298
Hpy188I TCNGA 3 cut(s) 67, 117, 504
Hpy188III TCNNGA 4 cut(s) 226, 429, 467, 517
Hpy8I GTNNAC 3 cut(s) 175, 262, 298
HpyAV CCTTC 2 cut(s) 392, 644
HpyCH4III ACNGT 2 cut(s) 17, 853
HpyCH4IV ACGT 2 cut(s) 771, 837
HpyCH4V TGCA 9 cut(s) 8, 146, 209, 221, 288, 370, 377, 696, 889
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 875
HpyF3I CTNAG 2 cut(s) 451, 776
HpySE526I ACGT 2 cut(s) 771, 837
Hsp92II CATG 6 cut(s) 170, 374, 470, 487, 617, 869
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 5 cut(s) 469, 504, 555, 862, 933
LmnI GCTCC 2 cut(s) 388, 431
Lsp1109I GCAGC 3 cut(s) 406, 446, 775
LweI GCATC 6 cut(s) 114, 364, 585, 751, 789, 834
MaeI CTAG 2 cut(s) 564, 937
MaeII ACGT 2 cut(s) 771, 837
MaeIII GTNAC 2 cut(s) 34, 195
MalI GATC 5 cut(s) 471, 506, 557, 864, 935
MboI GATC 5 cut(s) 469, 504, 555, 862, 933
MboII GAAGA 5 cut(s) 56, 116, 317, 683, 806
MflI RGATCY 1 cut(s) 933
MluCI AATT 9 cut(s) 60, 72, 241, 252, 308, 525, 639, 699, 708
MlyI GAGTC 1 cut(s) 238
MseI TTAA 3 cut(s) 80, 321, 336
MslI CAYNNNNRTG 2 cut(s) 32, 488
MspA1I CMGCKG 1 cut(s) 41
MspR9I CCNGG 3 cut(s) 804, 833, 871
Mva1269I GAATGC 1 cut(s) 223
MvaI CCWGG 3 cut(s) 804, 833, 871
MwoI GCNNNNNNNGC 2 cut(s) 47, 875
NcoI CCATGG 1 cut(s) 483
NdeII GATC 5 cut(s) 469, 504, 555, 862, 933
NlaIII CATG 6 cut(s) 170, 374, 470, 487, 617, 869
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 34
PagI TCATGA 1 cut(s) 466
PctI GAATGC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 151, 479
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 3 cut(s) 421, 436, 790
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PsiI TTATAA 1 cut(s) 638
Psp6I CCWGG 3 cut(s) 802, 831, 869
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 3 cut(s) 802, 831, 869
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 1 cut(s) 298
PsrI GAACNNNNNNTAC 2 cut(s) 886, 918
PsuI RGATCY 1 cut(s) 933
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 904
RsaNI GTAC 1 cut(s) 903
RseI CAYNNNNRTG 2 cut(s) 32, 488
SaqAI TTAA 3 cut(s) 80, 321, 336
SatI GCNGC 3 cut(s) 420, 435, 789
Sau3AI GATC 5 cut(s) 469, 504, 555, 862, 933
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 3 cut(s) 804, 833, 871
SfaNI GCATC 6 cut(s) 114, 364, 585, 751, 789, 834
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 2 cut(s) 32, 488
SmlI CTYRAG 1 cut(s) 909
SmoI CTYRAG 1 cut(s) 909
Sse9I AATT 9 cut(s) 60, 72, 241, 252, 308, 525, 639, 699, 708
SsiI CCGC 2 cut(s) 329, 878
SspMI CTAG 2 cut(s) 564, 937
StyD4I CCNGG 3 cut(s) 802, 831, 869
StyI CCWWGG 2 cut(s) 483, 723
TaaI ACNGT 2 cut(s) 17, 853
TaiI ACGT 2 cut(s) 774, 840
TaqI TCGA 3 cut(s) 202, 518, 858
TaqII GACCGA 1 cut(s) 315
TasI AATT 9 cut(s) 60, 72, 241, 252, 308, 525, 639, 699, 708
TfiI GAWTC 2 cut(s) 151, 479
Tru1I TTAA 3 cut(s) 80, 321, 336
Tru9I TTAA 3 cut(s) 80, 321, 336
TscAI CASTG 2 cut(s) 368, 930
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 3 cut(s) 419, 434, 788
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 293, 419, 455, 663
TspRI CASTG 2 cut(s) 368, 930
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 5 cut(s) 60, 241, 308, 525, 708
XspI CTAG 2 cut(s) 564, 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.