Rh6AG322000
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0019828
Physical Location & Seq
Forward (+)
52218160 .. 52218615
456 bp
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UTR
Exon/CDS
Intron
Rh6AG322000.1

Sequence Viewer

Length: 456 bp
ATGGATGTGCTATCTATTCAGATCACCCCTCTCTCGATTGGAATTCGAGCTGATGGAAACAACTTTGTGCCGATCATACCTAGAAACACCACAATACCAGCCCAAAGAGAGATGCTTTTCACAACATCCCATGATAATCAAGCCGAAGCACTTATAATTGTTTATGAAGGTGATGGCAAGAAGCTGCAAGAAAATCATTTGCTGGGATACTGCAAAATTGAGGGAATTCCTCCAGCAACCAAGGGAGTTCCACAAATACGAGTAATGATGGACATTGATGCTTCAAATGTGCTGAGAGTTTTGGCTGCTGTGTTGATGCCAGGTTCTCATCTTCCAATAAATCCTGTCCTGGACGTGAGGATGCCAACGGTTGACGATGGTCATGCCTGGTGCGCGGAAGCCTTGCACAGAACTTATGGTGCTACTCAAGACTTGGTATCAATGAAGAGGATCTAG

Protein Analysis

151

Amino Acids

16.58

Weight (kDa)

5.9

Isoelectric Point (pI)

25.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 2 - 106 7.6e-25 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019828)

Species Orthologous Gene IDs
pyrus_communis pycom13g16470
rosa_chinensis RchiOBHm_Chr6g0290311
rosa_multiflora Rmu_sc0015642.1_g000001
rosa_roxburghii Rroxscaffold_7G00177170
rosa_samantha Rh6AG322000 Rh6CG335200
rosa_wichuraiana Rw6G027900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 155
AccII CGCG 1 cut(s) 395
AciI CCGC 1 cut(s) 395
AcsI RAATTY 2 cut(s) 42, 225
AgsI TTSAA 1 cut(s) 285
AjiI CACGTC 1 cut(s) 355
AjnI CCWGG 3 cut(s) 319, 348, 386
AluBI AGCT 2 cut(s) 50, 184
AluI AGCT 2 cut(s) 50, 184
ApeKI GCWGC 2 cut(s) 184, 305
ApoI RAATTY 2 cut(s) 42, 225
AspLEI GCGC 1 cut(s) 395
AsuHPI GGTGA 2 cut(s) 16, 182
BbvI GCAGC 2 cut(s) 171, 292
BccI CCATC 4 cut(s) 47, 167, 262, 371
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 3 cut(s) 321, 350, 388
BciVI GTATCC 1 cut(s) 200
BfaI CTAG 2 cut(s) 81, 454
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 2 cut(s) 185, 306
BlsI GCNGC 2 cut(s) 186, 307
Bme1390I CCNGG 3 cut(s) 321, 350, 388
BmgBI CACGTC 1 cut(s) 355
BmrFI CCNGG 3 cut(s) 321, 350, 388
BmsI GCATC 4 cut(s) 102, 268, 306, 351
BoxI GACNNNNGTC 1 cut(s) 378
BpmI CTGGAG 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 240
BseBI CCWGG 3 cut(s) 321, 350, 388
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 3 cut(s) 10, 125, 366
BseMII CTCAG 1 cut(s) 284
BseXI GCAGC 2 cut(s) 171, 292
BseYI CCCAGC 1 cut(s) 202
Bsh1236I CGCG 1 cut(s) 395
Bsp143I GATC 3 cut(s) 21, 72, 450
BspACI CCGC 1 cut(s) 395
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 3 cut(s) 21, 72, 450
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 3 cut(s) 321, 350, 388
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 1 cut(s) 440
BstDEI CTNAG 1 cut(s) 293
BstF5I GGATG 3 cut(s) 10, 125, 366
BstFNI CGCG 1 cut(s) 395
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 3 cut(s) 24, 75, 453
BstMBI GATC 3 cut(s) 21, 72, 450
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 3 cut(s) 321, 350, 388
BstPAI GACNNNNGTC 1 cut(s) 378
BstSCI CCNGG 3 cut(s) 319, 348, 386
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 171, 292
BstX2I RGATCY 1 cut(s) 450
BstYI RGATCY 1 cut(s) 450
BsuI GTATCC 1 cut(s) 200
BtrI CACGTC 1 cut(s) 355
BtsCI GGATG 3 cut(s) 10, 125, 366
CfoI GCGC 1 cut(s) 395
CviAII CATG 2 cut(s) 131, 383
CviJI RGCY 6 cut(s) 50, 101, 143, 184, 305, 401
CviKI_1 RGCY 6 cut(s) 50, 101, 143, 184, 305, 401
DdeI CTNAG 1 cut(s) 293
DpnI GATC 3 cut(s) 23, 74, 452
DpnII GATC 3 cut(s) 21, 72, 450
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 240
EcoRI GAATTC 2 cut(s) 42, 225
EcoRII CCWGG 3 cut(s) 319, 348, 386
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 134, 386
FaiI YATR 6 cut(s) 77, 132, 155, 165, 384, 417
FatI CATG 2 cut(s) 130, 382
Fnu4HI GCNGC 2 cut(s) 185, 306
FokI GGATG 3 cut(s) 17, 112, 373
Fsp4HI GCNGC 2 cut(s) 185, 306
FspBI CTAG 2 cut(s) 81, 454
GlaI GCGC 1 cut(s) 394
GluI GCNGC 2 cut(s) 185, 306
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 216
HhaI GCGC 1 cut(s) 395
Hin1II CATG 2 cut(s) 134, 386
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HphI GGTGA 2 cut(s) 16, 182
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 2 cut(s) 34, 428
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 3 cut(s) 187, 213, 406
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 1 cut(s) 293
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 2 cut(s) 134, 386
HspAI GCGC 1 cut(s) 393
Kzo9I GATC 3 cut(s) 21, 72, 450
Lsp1109I GCAGC 2 cut(s) 171, 292
LweI GCATC 4 cut(s) 102, 268, 306, 351
MaeI CTAG 2 cut(s) 81, 454
MaeII ACGT 1 cut(s) 354
MalI GATC 3 cut(s) 23, 74, 452
MboI GATC 3 cut(s) 21, 72, 450
MboII GAAGA 1 cut(s) 323
MflI RGATCY 1 cut(s) 450
MluCI AATT 4 cut(s) 42, 156, 216, 225
MnlI CCTC 5 cut(s) 39, 214, 240, 351, 441
MspR9I CCNGG 3 cut(s) 321, 350, 388
MvaI CCWGG 3 cut(s) 321, 350, 388
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeII GATC 3 cut(s) 21, 72, 450
NlaIII CATG 2 cut(s) 134, 386
PfoI TCCNGGA 1 cut(s) 348
PkrI GCNGC 2 cut(s) 186, 307
PshAI GACNNNNGTC 1 cut(s) 378
PsiI TTATAA 1 cut(s) 155
Psp6I CCWGG 3 cut(s) 319, 348, 386
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 3 cut(s) 319, 348, 386
PsuI RGATCY 1 cut(s) 450
SatI GCNGC 2 cut(s) 185, 306
Sau3AI GATC 3 cut(s) 21, 72, 450
ScrFI CCNGG 3 cut(s) 321, 350, 388
SetI ASST 6 cut(s) 52, 82, 172, 186, 325, 357
SfaNI GCATC 4 cut(s) 102, 268, 306, 351
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 4 cut(s) 42, 156, 216, 225
SsiI CCGC 1 cut(s) 395
SspMI CTAG 2 cut(s) 81, 454
StyD4I CCNGG 3 cut(s) 319, 348, 386
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 1 cut(s) 357
TaqI TCGA 2 cut(s) 35, 46
TasI AATT 4 cut(s) 42, 156, 216, 225
TseI GCWGC 2 cut(s) 184, 305
TspDTI ATGAA 1 cut(s) 180
XapI RAATTY 2 cut(s) 42, 225
XspI CTAG 2 cut(s) 81, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.